Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Maria Gruenberg, Clara Antunes Moniz, Natalie Ellen Hofmann, Rahel Wampfler, Cristian Koepfli, Ivo Mueller, Wuelton Marcelo Monteiro, Marcus Lacerda, Gisely Cardoso de Melo, Andrea Kuehn, Andre M. Siqueira, Ingrid Felger

ABSTRACT

A distinctive feature of Plasmodium vivax infections is the overall low parasite density in peripheral blood. Thus, identifying asymptomatic infected individuals in endemic communities requires diagnostic tests with high sensitivity. The detection limits of molecular diagnostic tests are primarily defined by the volume of blood analysed and by the copy number of the amplified molecular marker serving as the template for amplification. By using mitochondrial DNA as the multi-copy template, the detection limit can be improved more than tenfold, compared to standard 18S rRNA targets, thereby allowing detection of lower parasite densities. In a very low transmission area in Brazil, application of a mitochondrial DNA-based assay increased prevalence from 4.9 to 6.5%. The usefulness of molecular tests in malaria epidemiological studies is widely recognized, especially when precise prevalence rates are desired. Of concern, however, is the challenge of demonstrating test accuracy and quality control for samples with very low parasite densities. In this case, chance effects in template distribution around the detection limit constrain reproducibility. Rigorous assessment of false positive and false negative test results is, therefore, required to prevent over- or under-estimation of parasite prevalence in epidemiological studies or when monitoring interventions. More... »

PAGES

55

References to SciGraph publications

  • 2015-12. Loop-mediated isothermal amplification (LAMP) for point-of-care detection of asymptomatic low-density malaria parasite carriers in Zanzibar in MALARIA JOURNAL
  • 2017-12. Very high carriage of gametocytes in asymptomatic low-density Plasmodium falciparum and P. vivax infections in western Thailand in FILARIA JOURNAL
  • 2013-12. A new computational approach redefines the subtelomeric vir superfamily of Plasmodium vivax in BMC GENOMICS
  • 2009-12. Towards high-throughput molecular detection of Plasmodium: new approaches and molecular markers in MALARIA JOURNAL
  • 2012-12. Real-time PCR diagnosis of Plasmodium vivax among blood donors in MALARIA JOURNAL
  • 2009-12. Implementation of a novel PCR based method for detecting malaria parasites from naturally infected mosquitoes in Papua New Guinea in MALARIA JOURNAL
  • 2015-12. An ultrasensitive reverse transcription polymerase chain reaction assay to detect asymptomatic low-density Plasmodium falciparum and Plasmodium vivax infections in small volume blood samples in MALARIA JOURNAL
  • 2017-12. The shape of the iceberg: quantification of submicroscopic Plasmodium falciparum and Plasmodium vivax parasitaemia and gametocytaemia in five low endemic settings in Ethiopia in MALARIA JOURNAL
  • 2017-12. Whole-genome sequencing of a Plasmodium vivax clinical isolate exhibits geographical characteristics and high genetic variation in China-Myanmar border area in BMC GENOMICS
  • 2013-12. A novel, single-amplification PCR targeting mitochondrial genome highly sensitive and specific in diagnosing malaria among returned travellers in Bergen, Norway in MALARIA JOURNAL
  • 2016-12. Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR) in SCIENTIFIC REPORTS
  • 2015-12. A systematic review of sub-microscopic Plasmodium vivax infection in MALARIA JOURNAL
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12936-018-2201-0

    DOI

    http://dx.doi.org/10.1186/s12936-018-2201-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1100688641

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29378609


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Basel", 
              "id": "https://www.grid.ac/institutes/grid.6612.3", 
              "name": [
                "Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland", 
                "University of Basel, Basel, Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gruenberg", 
            "givenName": "Maria", 
            "id": "sg:person.011275340330.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011275340330.13"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Basel", 
              "id": "https://www.grid.ac/institutes/grid.6612.3", 
              "name": [
                "Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland", 
                "University of Basel, Basel, Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Moniz", 
            "givenName": "Clara Antunes", 
            "id": "sg:person.012072720730.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012072720730.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Basel", 
              "id": "https://www.grid.ac/institutes/grid.6612.3", 
              "name": [
                "Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland", 
                "University of Basel, Basel, Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hofmann", 
            "givenName": "Natalie Ellen", 
            "id": "sg:person.01300553246.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300553246.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Basel", 
              "id": "https://www.grid.ac/institutes/grid.6612.3", 
              "name": [
                "Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland", 
                "University of Basel, Basel, Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wampfler", 
            "givenName": "Rahel", 
            "id": "sg:person.01047201020.56", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047201020.56"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Walter and Eliza Hall Institute of Medical Research", 
              "id": "https://www.grid.ac/institutes/grid.1042.7", 
              "name": [
                "Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Koepfli", 
            "givenName": "Cristian", 
            "id": "sg:person.0656477236.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0656477236.27"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Walter and Eliza Hall Institute of Medical Research", 
              "id": "https://www.grid.ac/institutes/grid.1042.7", 
              "name": [
                "Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mueller", 
            "givenName": "Ivo", 
            "id": "sg:person.0763540325.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0763540325.40"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Funda\u00e7\u00e3o de Medicina Tropical", 
              "id": "https://www.grid.ac/institutes/grid.418153.a", 
              "name": [
                "Funda\u00e7\u00e3o de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus, Brazil"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Monteiro", 
            "givenName": "Wuelton Marcelo", 
            "id": "sg:person.01141171601.76", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01141171601.76"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of the State of Amazonas", 
              "id": "https://www.grid.ac/institutes/grid.412290.c", 
              "name": [
                "Funda\u00e7\u00e3o de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus, Brazil", 
                "Universidade do Estado do Amazonas, Manaus, Brazil"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lacerda", 
            "givenName": "Marcus", 
            "id": "sg:person.016300655744.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016300655744.42"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of the State of Amazonas", 
              "id": "https://www.grid.ac/institutes/grid.412290.c", 
              "name": [
                "Funda\u00e7\u00e3o de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus, Brazil", 
                "Universidade do Estado do Amazonas, Manaus, Brazil"
              ], 
              "type": "Organization"
            }, 
            "familyName": "de Melo", 
            "givenName": "Gisely Cardoso", 
            "id": "sg:person.01060624443.99", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01060624443.99"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Funda\u00e7\u00e3o de Medicina Tropical", 
              "id": "https://www.grid.ac/institutes/grid.418153.a", 
              "name": [
                "Funda\u00e7\u00e3o de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus, Brazil"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kuehn", 
            "givenName": "Andrea", 
            "id": "sg:person.01070635024.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070635024.03"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Oswaldo Cruz Foundation", 
              "id": "https://www.grid.ac/institutes/grid.418068.3", 
              "name": [
                "Funda\u00e7\u00e3o de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus, Brazil", 
                "Instituto Nacional de Infectologia, Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Siqueira", 
            "givenName": "Andre M.", 
            "id": "sg:person.01036270546.07", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036270546.07"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Basel", 
              "id": "https://www.grid.ac/institutes/grid.6612.3", 
              "name": [
                "Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland", 
                "University of Basel, Basel, Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Felger", 
            "givenName": "Ingrid", 
            "id": "sg:person.01054613230.24", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054613230.24"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1093/infdis/jiv596", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002328860"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-015-1038-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002358283", 
              "https://doi.org/10.1186/s12936-015-1038-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/infdis/jiu018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005801092"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.actatropica.2009.02.003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006189002"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pntd.0003758", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007551401"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1373/clinchem.2008.112797", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007780642"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jcm.00355-10", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008358746"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/infdis/jit184", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013472229"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-12-26", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016660983", 
              "https://doi.org/10.1186/1475-2875-12-26"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep39183", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017240777", 
              "https://doi.org/10.1038/srep39183"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-015-0573-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018055401", 
              "https://doi.org/10.1186/s12936-015-0573-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-015-0573-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018055401", 
              "https://doi.org/10.1186/s12936-015-0573-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4269/ajtmh.2012.10-0658", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018657036"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-11-345", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018710510", 
              "https://doi.org/10.1186/1475-2875-11-345"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pmed.1001788", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019250716"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pntd.0003413", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020337280"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0076316", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025818681"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0009047", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029826131"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pntd.0002569", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031189229"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-015-0884-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031830037", 
              "https://doi.org/10.1186/s12936-015-0884-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jcm.02117-06", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032274171"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pntd.0004443", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033048585"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2164-14-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034062526", 
              "https://doi.org/10.1186/1471-2164-14-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pntd.0003109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039310095"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jcm.00276-11", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040797150"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-8-86", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042428786", 
              "https://doi.org/10.1186/1475-2875-8-86"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.parint.2014.09.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042764453"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4269/ajtmh.12-0581", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043535433"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4269/ajtmh.12-0581", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043535433"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4269/ajtmh.12-0581", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043535433"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/jmbi.1997.1038", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044752686"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jcm.02603-10", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045134677"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jcm.01057-14", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046052215"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ijpara.2016.10.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046365439"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ijpara.2016.10.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046365439"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0054986", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047837429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/cmr.00051-10", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048527307"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0126747", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050847668"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-8-182", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052530795", 
              "https://doi.org/10.1186/1475-2875-8-182"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.12688/wellcomeopenres.9876.1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1064613012"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.12688/wellcomeopenres.9876.1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1064613012"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4238/2012.september.25.9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072391223"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2144/0000113902", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1078629379"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1996.tb00401.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1082875658"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083073473", 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12864-017-3523-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083687280", 
              "https://doi.org/10.1186/s12864-017-3523-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12864-017-3523-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083687280", 
              "https://doi.org/10.1186/s12864-017-3523-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-017-1749-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084251200", 
              "https://doi.org/10.1186/s12936-017-1749-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-017-1749-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084251200", 
              "https://doi.org/10.1186/s12936-017-1749-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pntd.0005516", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084509258"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pntd.0005753", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090852960"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.7554/elife.23708", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091408682"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/infdis/jix507", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091867109"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4269/ajtmh.17-0225", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092162093"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13071-017-2407-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092342957", 
              "https://doi.org/10.1186/s13071-017-2407-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pmed.1002452", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1093089239"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2018-12", 
        "datePublishedReg": "2018-12-01", 
        "description": "A distinctive feature of Plasmodium vivax infections is the overall low parasite density in peripheral blood. Thus, identifying asymptomatic infected individuals in endemic communities requires diagnostic tests with high sensitivity. The detection limits of molecular diagnostic tests are primarily defined by the volume of blood analysed and by the copy number of the amplified molecular marker serving as the template for amplification. By using mitochondrial DNA as the multi-copy template, the detection limit can be improved more than tenfold, compared to standard 18S rRNA targets, thereby allowing detection of lower parasite densities. In a very low transmission area in Brazil, application of a mitochondrial DNA-based\u00a0assay increased prevalence from 4.9 to 6.5%. The usefulness of molecular tests in malaria epidemiological studies is widely recognized, especially when precise prevalence rates are desired. Of concern, however, is the challenge of demonstrating test accuracy and quality control for samples with very low parasite densities. In this case, chance effects in template distribution around the detection limit constrain reproducibility. Rigorous assessment of false positive and false negative test results is, therefore, required to prevent over- or under-estimation of parasite prevalence in epidemiological studies or when monitoring interventions.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1186/s12936-018-2201-0", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.5239743", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.6661937", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1030597", 
            "issn": [
              "1475-2875"
            ], 
            "name": "Malaria Journal", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "17"
          }
        ], 
        "name": "Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions", 
        "pagination": "55", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "491b3e6d256340c32b83bb5c6578b3453cec46a300fba025c662c0b09bdf9213"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "29378609"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101139802"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s12936-018-2201-0"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1100688641"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s12936-018-2201-0", 
          "https://app.dimensions.ai/details/publication/pub.1100688641"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T01:55", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000493.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1186/s12936-018-2201-0"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12936-018-2201-0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12936-018-2201-0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12936-018-2201-0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12936-018-2201-0'


     

    This table displays all metadata directly associated to this object as RDF triples.

    322 TRIPLES      21 PREDICATES      78 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s12936-018-2201-0 schema:about anzsrc-for:11
    2 anzsrc-for:1108
    3 schema:author Na4805731f20c42b3afec96dbb7dc516a
    4 schema:citation sg:pub.10.1038/srep39183
    5 sg:pub.10.1186/1471-2164-14-8
    6 sg:pub.10.1186/1475-2875-11-345
    7 sg:pub.10.1186/1475-2875-12-26
    8 sg:pub.10.1186/1475-2875-8-182
    9 sg:pub.10.1186/1475-2875-8-86
    10 sg:pub.10.1186/s12864-017-3523-y
    11 sg:pub.10.1186/s12936-015-0573-y
    12 sg:pub.10.1186/s12936-015-0884-z
    13 sg:pub.10.1186/s12936-015-1038-z
    14 sg:pub.10.1186/s12936-017-1749-4
    15 sg:pub.10.1186/s13071-017-2407-y
    16 https://app.dimensions.ai/details/publication/pub.1083073473
    17 https://doi.org/10.1002/j.1460-2075.1996.tb00401.x
    18 https://doi.org/10.1006/jmbi.1997.1038
    19 https://doi.org/10.1016/j.actatropica.2009.02.003
    20 https://doi.org/10.1016/j.ijpara.2016.10.006
    21 https://doi.org/10.1016/j.parint.2014.09.006
    22 https://doi.org/10.1093/infdis/jit184
    23 https://doi.org/10.1093/infdis/jiu018
    24 https://doi.org/10.1093/infdis/jiv596
    25 https://doi.org/10.1093/infdis/jix507
    26 https://doi.org/10.1128/cmr.00051-10
    27 https://doi.org/10.1128/jcm.00276-11
    28 https://doi.org/10.1128/jcm.00355-10
    29 https://doi.org/10.1128/jcm.01057-14
    30 https://doi.org/10.1128/jcm.02117-06
    31 https://doi.org/10.1128/jcm.02603-10
    32 https://doi.org/10.12688/wellcomeopenres.9876.1
    33 https://doi.org/10.1371/journal.pmed.1001788
    34 https://doi.org/10.1371/journal.pmed.1002452
    35 https://doi.org/10.1371/journal.pntd.0002569
    36 https://doi.org/10.1371/journal.pntd.0003109
    37 https://doi.org/10.1371/journal.pntd.0003413
    38 https://doi.org/10.1371/journal.pntd.0003758
    39 https://doi.org/10.1371/journal.pntd.0004443
    40 https://doi.org/10.1371/journal.pntd.0005516
    41 https://doi.org/10.1371/journal.pntd.0005753
    42 https://doi.org/10.1371/journal.pone.0009047
    43 https://doi.org/10.1371/journal.pone.0054986
    44 https://doi.org/10.1371/journal.pone.0076316
    45 https://doi.org/10.1371/journal.pone.0126747
    46 https://doi.org/10.1373/clinchem.2008.112797
    47 https://doi.org/10.2144/0000113902
    48 https://doi.org/10.4238/2012.september.25.9
    49 https://doi.org/10.4269/ajtmh.12-0581
    50 https://doi.org/10.4269/ajtmh.17-0225
    51 https://doi.org/10.4269/ajtmh.2012.10-0658
    52 https://doi.org/10.7554/elife.23708
    53 schema:datePublished 2018-12
    54 schema:datePublishedReg 2018-12-01
    55 schema:description A distinctive feature of Plasmodium vivax infections is the overall low parasite density in peripheral blood. Thus, identifying asymptomatic infected individuals in endemic communities requires diagnostic tests with high sensitivity. The detection limits of molecular diagnostic tests are primarily defined by the volume of blood analysed and by the copy number of the amplified molecular marker serving as the template for amplification. By using mitochondrial DNA as the multi-copy template, the detection limit can be improved more than tenfold, compared to standard 18S rRNA targets, thereby allowing detection of lower parasite densities. In a very low transmission area in Brazil, application of a mitochondrial DNA-based assay increased prevalence from 4.9 to 6.5%. The usefulness of molecular tests in malaria epidemiological studies is widely recognized, especially when precise prevalence rates are desired. Of concern, however, is the challenge of demonstrating test accuracy and quality control for samples with very low parasite densities. In this case, chance effects in template distribution around the detection limit constrain reproducibility. Rigorous assessment of false positive and false negative test results is, therefore, required to prevent over- or under-estimation of parasite prevalence in epidemiological studies or when monitoring interventions.
    56 schema:genre research_article
    57 schema:inLanguage en
    58 schema:isAccessibleForFree true
    59 schema:isPartOf N33c50ce263b24efba2c2f241585db822
    60 N9e9983d30d2a4013a650e238524ca303
    61 sg:journal.1030597
    62 schema:name Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions
    63 schema:pagination 55
    64 schema:productId Na28d3b6c17cf46e583d28a49b4e27d99
    65 Nb412a9b0a4e04cf5ac49140a4026ce1b
    66 Nb4994c5d4ea84fee9d0aa5b8a25b2453
    67 Ne5edabf790204d8b9d476a40ea85a3eb
    68 Nf2c093df48704487b509b4a1f0b96e30
    69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100688641
    70 https://doi.org/10.1186/s12936-018-2201-0
    71 schema:sdDatePublished 2019-04-11T01:55
    72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    73 schema:sdPublisher Ncd1a08c79ac943b0b8117c7e74276154
    74 schema:url http://link.springer.com/10.1186/s12936-018-2201-0
    75 sgo:license sg:explorer/license/
    76 sgo:sdDataset articles
    77 rdf:type schema:ScholarlyArticle
    78 N33c50ce263b24efba2c2f241585db822 schema:volumeNumber 17
    79 rdf:type schema:PublicationVolume
    80 N3c2fdea194034059b3d717d3de41df44 rdf:first sg:person.016300655744.42
    81 rdf:rest Nebe11f5d65fe45f58f9bae52ae819cf1
    82 N487032c3713b4d2192b5504d429ceb29 rdf:first sg:person.0763540325.40
    83 rdf:rest Ne25d561701c544fbaf151a945ef31942
    84 N56d10248521f48caa3778b8a64f1bb2f rdf:first sg:person.01054613230.24
    85 rdf:rest rdf:nil
    86 N73a26276f8a74ceab1f3ecefc72ada7d rdf:first sg:person.01070635024.03
    87 rdf:rest Nddea0e6c0bfd4bc396149644baa4ebdd
    88 N9b555920d66544a8b75c74b9119290a0 rdf:first sg:person.012072720730.63
    89 rdf:rest Nf7d3d0bcf4cc4d78998cedcbcf5acc76
    90 N9e9983d30d2a4013a650e238524ca303 schema:issueNumber 1
    91 rdf:type schema:PublicationIssue
    92 Na28d3b6c17cf46e583d28a49b4e27d99 schema:name readcube_id
    93 schema:value 491b3e6d256340c32b83bb5c6578b3453cec46a300fba025c662c0b09bdf9213
    94 rdf:type schema:PropertyValue
    95 Na4805731f20c42b3afec96dbb7dc516a rdf:first sg:person.011275340330.13
    96 rdf:rest N9b555920d66544a8b75c74b9119290a0
    97 Nb412a9b0a4e04cf5ac49140a4026ce1b schema:name doi
    98 schema:value 10.1186/s12936-018-2201-0
    99 rdf:type schema:PropertyValue
    100 Nb4994c5d4ea84fee9d0aa5b8a25b2453 schema:name dimensions_id
    101 schema:value pub.1100688641
    102 rdf:type schema:PropertyValue
    103 Ncd1a08c79ac943b0b8117c7e74276154 schema:name Springer Nature - SN SciGraph project
    104 rdf:type schema:Organization
    105 Nda92ea793a9a4412a7fbcb2960838dd3 rdf:first sg:person.01047201020.56
    106 rdf:rest Nf2c243be670644d2b584701a2cda03ba
    107 Nddea0e6c0bfd4bc396149644baa4ebdd rdf:first sg:person.01036270546.07
    108 rdf:rest N56d10248521f48caa3778b8a64f1bb2f
    109 Ne25d561701c544fbaf151a945ef31942 rdf:first sg:person.01141171601.76
    110 rdf:rest N3c2fdea194034059b3d717d3de41df44
    111 Ne5edabf790204d8b9d476a40ea85a3eb schema:name nlm_unique_id
    112 schema:value 101139802
    113 rdf:type schema:PropertyValue
    114 Nebe11f5d65fe45f58f9bae52ae819cf1 rdf:first sg:person.01060624443.99
    115 rdf:rest N73a26276f8a74ceab1f3ecefc72ada7d
    116 Nf2c093df48704487b509b4a1f0b96e30 schema:name pubmed_id
    117 schema:value 29378609
    118 rdf:type schema:PropertyValue
    119 Nf2c243be670644d2b584701a2cda03ba rdf:first sg:person.0656477236.27
    120 rdf:rest N487032c3713b4d2192b5504d429ceb29
    121 Nf7d3d0bcf4cc4d78998cedcbcf5acc76 rdf:first sg:person.01300553246.35
    122 rdf:rest Nda92ea793a9a4412a7fbcb2960838dd3
    123 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    124 schema:name Medical and Health Sciences
    125 rdf:type schema:DefinedTerm
    126 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
    127 schema:name Medical Microbiology
    128 rdf:type schema:DefinedTerm
    129 sg:grant.5239743 http://pending.schema.org/fundedItem sg:pub.10.1186/s12936-018-2201-0
    130 rdf:type schema:MonetaryGrant
    131 sg:grant.6661937 http://pending.schema.org/fundedItem sg:pub.10.1186/s12936-018-2201-0
    132 rdf:type schema:MonetaryGrant
    133 sg:journal.1030597 schema:issn 1475-2875
    134 schema:name Malaria Journal
    135 rdf:type schema:Periodical
    136 sg:person.01036270546.07 schema:affiliation https://www.grid.ac/institutes/grid.418068.3
    137 schema:familyName Siqueira
    138 schema:givenName Andre M.
    139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036270546.07
    140 rdf:type schema:Person
    141 sg:person.01047201020.56 schema:affiliation https://www.grid.ac/institutes/grid.6612.3
    142 schema:familyName Wampfler
    143 schema:givenName Rahel
    144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047201020.56
    145 rdf:type schema:Person
    146 sg:person.01054613230.24 schema:affiliation https://www.grid.ac/institutes/grid.6612.3
    147 schema:familyName Felger
    148 schema:givenName Ingrid
    149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054613230.24
    150 rdf:type schema:Person
    151 sg:person.01060624443.99 schema:affiliation https://www.grid.ac/institutes/grid.412290.c
    152 schema:familyName de Melo
    153 schema:givenName Gisely Cardoso
    154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01060624443.99
    155 rdf:type schema:Person
    156 sg:person.01070635024.03 schema:affiliation https://www.grid.ac/institutes/grid.418153.a
    157 schema:familyName Kuehn
    158 schema:givenName Andrea
    159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070635024.03
    160 rdf:type schema:Person
    161 sg:person.011275340330.13 schema:affiliation https://www.grid.ac/institutes/grid.6612.3
    162 schema:familyName Gruenberg
    163 schema:givenName Maria
    164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011275340330.13
    165 rdf:type schema:Person
    166 sg:person.01141171601.76 schema:affiliation https://www.grid.ac/institutes/grid.418153.a
    167 schema:familyName Monteiro
    168 schema:givenName Wuelton Marcelo
    169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01141171601.76
    170 rdf:type schema:Person
    171 sg:person.012072720730.63 schema:affiliation https://www.grid.ac/institutes/grid.6612.3
    172 schema:familyName Moniz
    173 schema:givenName Clara Antunes
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012072720730.63
    175 rdf:type schema:Person
    176 sg:person.01300553246.35 schema:affiliation https://www.grid.ac/institutes/grid.6612.3
    177 schema:familyName Hofmann
    178 schema:givenName Natalie Ellen
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300553246.35
    180 rdf:type schema:Person
    181 sg:person.016300655744.42 schema:affiliation https://www.grid.ac/institutes/grid.412290.c
    182 schema:familyName Lacerda
    183 schema:givenName Marcus
    184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016300655744.42
    185 rdf:type schema:Person
    186 sg:person.0656477236.27 schema:affiliation https://www.grid.ac/institutes/grid.1042.7
    187 schema:familyName Koepfli
    188 schema:givenName Cristian
    189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0656477236.27
    190 rdf:type schema:Person
    191 sg:person.0763540325.40 schema:affiliation https://www.grid.ac/institutes/grid.1042.7
    192 schema:familyName Mueller
    193 schema:givenName Ivo
    194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0763540325.40
    195 rdf:type schema:Person
    196 sg:pub.10.1038/srep39183 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017240777
    197 https://doi.org/10.1038/srep39183
    198 rdf:type schema:CreativeWork
    199 sg:pub.10.1186/1471-2164-14-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034062526
    200 https://doi.org/10.1186/1471-2164-14-8
    201 rdf:type schema:CreativeWork
    202 sg:pub.10.1186/1475-2875-11-345 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018710510
    203 https://doi.org/10.1186/1475-2875-11-345
    204 rdf:type schema:CreativeWork
    205 sg:pub.10.1186/1475-2875-12-26 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016660983
    206 https://doi.org/10.1186/1475-2875-12-26
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1186/1475-2875-8-182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052530795
    209 https://doi.org/10.1186/1475-2875-8-182
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1186/1475-2875-8-86 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042428786
    212 https://doi.org/10.1186/1475-2875-8-86
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1186/s12864-017-3523-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1083687280
    215 https://doi.org/10.1186/s12864-017-3523-y
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1186/s12936-015-0573-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1018055401
    218 https://doi.org/10.1186/s12936-015-0573-y
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1186/s12936-015-0884-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1031830037
    221 https://doi.org/10.1186/s12936-015-0884-z
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1186/s12936-015-1038-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1002358283
    224 https://doi.org/10.1186/s12936-015-1038-z
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1186/s12936-017-1749-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084251200
    227 https://doi.org/10.1186/s12936-017-1749-4
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1186/s13071-017-2407-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1092342957
    230 https://doi.org/10.1186/s13071-017-2407-y
    231 rdf:type schema:CreativeWork
    232 https://app.dimensions.ai/details/publication/pub.1083073473 schema:CreativeWork
    233 https://doi.org/10.1002/j.1460-2075.1996.tb00401.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082875658
    234 rdf:type schema:CreativeWork
    235 https://doi.org/10.1006/jmbi.1997.1038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044752686
    236 rdf:type schema:CreativeWork
    237 https://doi.org/10.1016/j.actatropica.2009.02.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006189002
    238 rdf:type schema:CreativeWork
    239 https://doi.org/10.1016/j.ijpara.2016.10.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046365439
    240 rdf:type schema:CreativeWork
    241 https://doi.org/10.1016/j.parint.2014.09.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042764453
    242 rdf:type schema:CreativeWork
    243 https://doi.org/10.1093/infdis/jit184 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013472229
    244 rdf:type schema:CreativeWork
    245 https://doi.org/10.1093/infdis/jiu018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005801092
    246 rdf:type schema:CreativeWork
    247 https://doi.org/10.1093/infdis/jiv596 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002328860
    248 rdf:type schema:CreativeWork
    249 https://doi.org/10.1093/infdis/jix507 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091867109
    250 rdf:type schema:CreativeWork
    251 https://doi.org/10.1128/cmr.00051-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048527307
    252 rdf:type schema:CreativeWork
    253 https://doi.org/10.1128/jcm.00276-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040797150
    254 rdf:type schema:CreativeWork
    255 https://doi.org/10.1128/jcm.00355-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008358746
    256 rdf:type schema:CreativeWork
    257 https://doi.org/10.1128/jcm.01057-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046052215
    258 rdf:type schema:CreativeWork
    259 https://doi.org/10.1128/jcm.02117-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032274171
    260 rdf:type schema:CreativeWork
    261 https://doi.org/10.1128/jcm.02603-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045134677
    262 rdf:type schema:CreativeWork
    263 https://doi.org/10.12688/wellcomeopenres.9876.1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064613012
    264 rdf:type schema:CreativeWork
    265 https://doi.org/10.1371/journal.pmed.1001788 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019250716
    266 rdf:type schema:CreativeWork
    267 https://doi.org/10.1371/journal.pmed.1002452 schema:sameAs https://app.dimensions.ai/details/publication/pub.1093089239
    268 rdf:type schema:CreativeWork
    269 https://doi.org/10.1371/journal.pntd.0002569 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031189229
    270 rdf:type schema:CreativeWork
    271 https://doi.org/10.1371/journal.pntd.0003109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039310095
    272 rdf:type schema:CreativeWork
    273 https://doi.org/10.1371/journal.pntd.0003413 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020337280
    274 rdf:type schema:CreativeWork
    275 https://doi.org/10.1371/journal.pntd.0003758 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007551401
    276 rdf:type schema:CreativeWork
    277 https://doi.org/10.1371/journal.pntd.0004443 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033048585
    278 rdf:type schema:CreativeWork
    279 https://doi.org/10.1371/journal.pntd.0005516 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084509258
    280 rdf:type schema:CreativeWork
    281 https://doi.org/10.1371/journal.pntd.0005753 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090852960
    282 rdf:type schema:CreativeWork
    283 https://doi.org/10.1371/journal.pone.0009047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029826131
    284 rdf:type schema:CreativeWork
    285 https://doi.org/10.1371/journal.pone.0054986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047837429
    286 rdf:type schema:CreativeWork
    287 https://doi.org/10.1371/journal.pone.0076316 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025818681
    288 rdf:type schema:CreativeWork
    289 https://doi.org/10.1371/journal.pone.0126747 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050847668
    290 rdf:type schema:CreativeWork
    291 https://doi.org/10.1373/clinchem.2008.112797 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007780642
    292 rdf:type schema:CreativeWork
    293 https://doi.org/10.2144/0000113902 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078629379
    294 rdf:type schema:CreativeWork
    295 https://doi.org/10.4238/2012.september.25.9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072391223
    296 rdf:type schema:CreativeWork
    297 https://doi.org/10.4269/ajtmh.12-0581 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043535433
    298 rdf:type schema:CreativeWork
    299 https://doi.org/10.4269/ajtmh.17-0225 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092162093
    300 rdf:type schema:CreativeWork
    301 https://doi.org/10.4269/ajtmh.2012.10-0658 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018657036
    302 rdf:type schema:CreativeWork
    303 https://doi.org/10.7554/elife.23708 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091408682
    304 rdf:type schema:CreativeWork
    305 https://www.grid.ac/institutes/grid.1042.7 schema:alternateName Walter and Eliza Hall Institute of Medical Research
    306 schema:name Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia
    307 rdf:type schema:Organization
    308 https://www.grid.ac/institutes/grid.412290.c schema:alternateName University of the State of Amazonas
    309 schema:name Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus, Brazil
    310 Universidade do Estado do Amazonas, Manaus, Brazil
    311 rdf:type schema:Organization
    312 https://www.grid.ac/institutes/grid.418068.3 schema:alternateName Oswaldo Cruz Foundation
    313 schema:name Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus, Brazil
    314 Instituto Nacional de Infectologia, Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil
    315 rdf:type schema:Organization
    316 https://www.grid.ac/institutes/grid.418153.a schema:alternateName Fundação de Medicina Tropical
    317 schema:name Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus, Brazil
    318 rdf:type schema:Organization
    319 https://www.grid.ac/institutes/grid.6612.3 schema:alternateName University of Basel
    320 schema:name Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland
    321 University of Basel, Basel, Switzerland
    322 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...