Low-grade sulfadoxine–pyrimethamine resistance in Plasmodium falciparum parasites from Lubango, Angola View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-12

AUTHORS

Elsa P. S. Kaingona-Daniel, Larissa Rodrigues Gomes, Bianca E. Gama, Natália K. Almeida-de-Oliveira, Filomeno Fortes, Didier Ménard, Cláudio Tadeu Daniel-Ribeiro, Maria de Fátima Ferreira-da-Cruz

ABSTRACT

BACKGROUND: Malaria is a major parasitic disease, affecting millions of people in endemic areas. Plasmodium falciparum parasites are responsible for the most severe cases and its resistance to anti-malarial drugs is notorious. This is a possible obstacle to the effectiveness of intermittent preventive treatment (IPT) based on sulfadoxine-pyrimethamine (SP) cures administrated to pregnant women (IPTp) during their pregnancy. As this intervention is recommended in Angola since 2006, it has assessed, in this country, the molecular profiles in P. falciparum dhfr and dhps, two polymorphic genes associated to pyrimethamine and sulfadoxine resistance, respectively. METHODS: Blood samples from 52 falciparum patients were collected in Lubango, Angola and pfdhfr and pfdhps polymorphisms were analysed using nested-PCR and DNA sequencing. RESULTS: In the pfdhfr gene, the 108N mutation was almost fixed (98 %), followed by 59R (63 %), 51I (46 %), 50R and 164L (2 %, respectively). No 16V/S mutations were found. The most common double mutant genotype was CNRN (59 + 108; 46 %), followed by CICN (51 + 108; 29 %) whereas IRN (51 + 59 + 108; 15 %), CNRNVL (59 + 108 + 164; 2 %) and RICN (50 + 51 + 108; 2 %) triple mutant genotypes were detected. Investigations of the pfdhps gene showed that the 437G mutation was the most prevalent (97 %). Only two and one samples disclosed the 540E (7 %) and the 436A (3 %), respectively. Single mutant SGKAA (437; 86 %) was higher than SGEAA (437 + 540; 7 %) or AGKAA (436 + 437; 3 %) double mutants genotypes. No polymorphism was detected at codons 581G and 613T/S. Combining pfdhfr and pfdhps alleles two triple mutant haplotypes (double mutant in dhfr and single mutant in dhps) were observed: the ACICNVI/SGKAA in 14 (56 %) samples and the ACNRNVI/SGKAA in five (20 %) samples. One quadruple mutant haplotype was detected (ACIRNVI/SGKAA) in six (24 %) P. falciparum samples. No quintuple pfdhfr-pfdhps mutant was noted. CONCLUSION: pfdhfr and pfdhps gene mutations in isolates from Lubango are suggestive of a low-grade SP resistance and IPT for pregnant women and infant based on SP treatment could be effective. Routine molecular studies targeting polymorphism in these two genes need to be routinely conducted at country level. More... »

PAGES

309

References to SciGraph publications

  • 2011-12. The effectiveness and perception of the use of sulphadoxine-pyrimethamine in intermittent preventive treatment of malaria in pregnancy programme in Offinso district of ashanti region, Ghana in MALARIA JOURNAL
  • 2008-12. Molecular epidemiology of drug-resistant malaria in western Kenya highlands in BMC INFECTIOUS DISEASES
  • 2009-12. A systematic review and meta-analysis of evidence for correlation between molecular markers of parasite resistance and treatment outcome in falciparum malaria in MALARIA JOURNAL
  • 2015-12. Molecular epidemiology of drug-resistant Plasmodium falciparum in Benguela province, Angola in MALARIA JOURNAL
  • 2011-12. Evaluation of prevalence's of pfdhfr and pfdhps mutations in Angola in MALARIA JOURNAL
  • 2010-12. Anti-folate drug resistance in Africa: meta-analysis of reported dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) mutant genotype frequencies in African Plasmodium falciparum parasite populations in MALARIA JOURNAL
  • 1996-08. Malaria diagnosis: standardization of a polymerase chain reaction for the detection of Plasmodium falciparum parasites in individuals with low-grade parasitemia in PARASITOLOGY RESEARCH
  • 2011-12. Molecular markers of antifolate resistance in Plasmodium falciparum isolates from Luanda, Angola in MALARIA JOURNAL
  • 2008-12. Prevalence of pfmdr1, pfcrt, pfdhfr and pfdhps mutations associated with drug resistance, in Luanda, Angola in MALARIA JOURNAL
  • 2006-12. High prevalence of drug-resistance mutations in Plasmodium falciparum and Plasmodium vivax in southern Ethiopia in MALARIA JOURNAL
  • 2010-12. Tracing the origins and signatures of selection of antifolate resistance in island populations of Plasmodium falciparum in BMC INFECTIOUS DISEASES
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12936-016-1358-7

    DOI

    http://dx.doi.org/10.1186/s12936-016-1358-7

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1029478986

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27267365


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Angola", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Antimalarials", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Dihydropteroate Synthase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Drug Combinations", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Drug Resistance", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Malaria, Falciparum", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mutation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plasmodium falciparum", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymorphism, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protozoan Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pyrimethamine", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Analysis, DNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sulfadoxine", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tetrahydrofolate Dehydrogenase", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Oswaldo Cruz Foundation", 
              "id": "https://www.grid.ac/institutes/grid.418068.3", 
              "name": [
                "Laborat\u00f3rio de Pesquisa em Mal\u00e1ria\u2014Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil", 
                "Centro de Pesquisa, Diagn\u00f3stico e Treinamento em Mal\u00e1ria (CPD-Mal) Fiocruz, Rio de Janeiro, Brazil", 
                "Hospital Central Dr. Ant\u00f3nio Agostinho Neto, Lubango, Angola", 
                "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDESMal/CPLP), Lisbon, Portugal"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kaingona-Daniel", 
            "givenName": "Elsa P. S.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Oswaldo Cruz Foundation", 
              "id": "https://www.grid.ac/institutes/grid.418068.3", 
              "name": [
                "Laborat\u00f3rio de Pesquisa em Mal\u00e1ria\u2014Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil", 
                "Centro de Pesquisa, Diagn\u00f3stico e Treinamento em Mal\u00e1ria (CPD-Mal) Fiocruz, Rio de Janeiro, Brazil", 
                "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDESMal/CPLP), Lisbon, Portugal"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gomes", 
            "givenName": "Larissa Rodrigues", 
            "id": "sg:person.01261510365.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261510365.42"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Instituto Nacional do C\u00e2ncer", 
              "id": "https://www.grid.ac/institutes/grid.419166.d", 
              "name": [
                "Laborat\u00f3rio de Pesquisa em Mal\u00e1ria\u2014Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil", 
                "Laboratory of Oncovirology, Instituto Nacional de C\u00e2ncer, Rio de Janeiro, Brazil"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gama", 
            "givenName": "Bianca E.", 
            "id": "sg:person.01307357647.97", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307357647.97"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Oswaldo Cruz Foundation", 
              "id": "https://www.grid.ac/institutes/grid.418068.3", 
              "name": [
                "Laborat\u00f3rio de Pesquisa em Mal\u00e1ria\u2014Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil", 
                "Centro de Pesquisa, Diagn\u00f3stico e Treinamento em Mal\u00e1ria (CPD-Mal) Fiocruz, Rio de Janeiro, Brazil", 
                "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDESMal/CPLP), Lisbon, Portugal"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Almeida-de-Oliveira", 
            "givenName": "Nat\u00e1lia K.", 
            "id": "sg:person.01237167267.74", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237167267.74"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Angolan National Malaria Control Programme, National Institute of Public Health, Luanda, Angola", 
                "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDESMal/CPLP), Lisbon, Portugal"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Fortes", 
            "givenName": "Filomeno", 
            "id": "sg:person.01003711753.89", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003711753.89"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institut Pasteur du Cambodge", 
              "id": "https://www.grid.ac/institutes/grid.418537.c", 
              "name": [
                "Malaria Molecular Epidemiology Unit, Institut Pasteur in Cambodia, Phnom Penh, Cambodia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "M\u00e9nard", 
            "givenName": "Didier", 
            "id": "sg:person.0755351436.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755351436.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Oswaldo Cruz Foundation", 
              "id": "https://www.grid.ac/institutes/grid.418068.3", 
              "name": [
                "Laborat\u00f3rio de Pesquisa em Mal\u00e1ria\u2014Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil", 
                "Centro de Pesquisa, Diagn\u00f3stico e Treinamento em Mal\u00e1ria (CPD-Mal) Fiocruz, Rio de Janeiro, Brazil", 
                "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDESMal/CPLP), Lisbon, Portugal"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Daniel-Ribeiro", 
            "givenName": "Cl\u00e1udio Tadeu", 
            "id": "sg:person.01220713203.83", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220713203.83"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Oswaldo Cruz Foundation", 
              "id": "https://www.grid.ac/institutes/grid.418068.3", 
              "name": [
                "Laborat\u00f3rio de Pesquisa em Mal\u00e1ria\u2014Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil", 
                "Centro de Pesquisa, Diagn\u00f3stico e Treinamento em Mal\u00e1ria (CPD-Mal) Fiocruz, Rio de Janeiro, Brazil", 
                "Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDESMal/CPLP), Lisbon, Portugal"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ferreira-da-Cruz", 
            "givenName": "Maria de F\u00e1tima", 
            "id": "sg:person.010067171043.79", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010067171043.79"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1086/504687", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002637513"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-10-248", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004333404", 
              "https://doi.org/10.1186/1475-2875-10-248"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-9-247", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004545394", 
              "https://doi.org/10.1186/1475-2875-9-247"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.pt.2013.07.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006679019"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1086/587845", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007387705"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-7-236", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010316876", 
              "https://doi.org/10.1186/1475-2875-7-236"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1086/518512", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013987624"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0004364", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016260428"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-015-0634-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016316151", 
              "https://doi.org/10.1186/s12936-015-0634-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-015-0634-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016316151", 
              "https://doi.org/10.1186/s12936-015-0634-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3201/eid2008.131897", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016346861"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.87.8.3018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018549017"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0140-6736(03)12951-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018879851"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0140-6736(03)12951-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018879851"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-3156.2004.01239.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019298701"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-3156.2007.01904.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023497284"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-3156.2007.01904.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023497284"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1590/s0074-02762000000500020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024617218"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-10-22", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026962984", 
              "https://doi.org/10.1186/1475-2875-10-22"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1086/342946", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029635400"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-5-54", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030186746", 
              "https://doi.org/10.1186/1475-2875-5-54"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.actatropica.2007.04.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031371734"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1086/588198", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032103986"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.pt.2013.08.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037644778"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/aac.00498-09", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037804573"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.actatropica.2006.07.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037857315"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0012618", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038898955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/aac.47.4.1347-1354.2003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040132193"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s004360050173", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040895283", 
              "https://doi.org/10.1007/s004360050173"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-8-89", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041383647", 
              "https://doi.org/10.1186/1475-2875-8-89"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0166-6851(94)00207-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042312772"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/infdis/jiu836", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042920146"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-3156.2009.02369.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044331506"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-3156.2005.01471.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044901893"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-3156.2005.01471.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044901893"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.87.8.3014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046332862"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2334-8-105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049429367", 
              "https://doi.org/10.1186/1471-2334-8-105"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.meegid.2009.04.015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049881547"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-10-385", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050786193", 
              "https://doi.org/10.1186/1475-2875-10-385"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1432-1033.1994.00397.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051593980"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2334-10-163", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051632152", 
              "https://doi.org/10.1186/1471-2334-10-163"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/00034980120103234", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1058260561"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1098876", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062449701"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4238/vol7-3x-meeting04", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072396808"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/aac.43.9.2205", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1074515067"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4269/ajtmh.2005.73.1131", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1077161643"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4269/ajtmh.2006.74.205", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1077188629"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4269/ajtmh.2009.80.523", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1077882214"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2016-12", 
        "datePublishedReg": "2016-12-01", 
        "description": "BACKGROUND: Malaria is a major parasitic disease, affecting millions of people in endemic areas. Plasmodium falciparum parasites are responsible for the most severe cases and its resistance to anti-malarial drugs is notorious. This is a possible obstacle to the effectiveness of intermittent preventive treatment (IPT) based on sulfadoxine-pyrimethamine (SP) cures administrated to pregnant women (IPTp) during their pregnancy. As this intervention is recommended in Angola since 2006, it has assessed, in this country, the molecular profiles in P. falciparum dhfr and dhps, two polymorphic genes associated to pyrimethamine and sulfadoxine resistance, respectively.\nMETHODS: Blood samples from 52 falciparum patients were collected in Lubango, Angola and pfdhfr and pfdhps polymorphisms were analysed using nested-PCR and DNA sequencing.\nRESULTS: In the pfdhfr gene, the 108N mutation was almost fixed (98\u00a0%), followed by 59R (63\u00a0%), 51I (46\u00a0%), 50R and 164L (2\u00a0%, respectively). No 16V/S mutations were found. The most common double mutant genotype was CNRN (59\u00a0+\u00a0108; 46\u00a0%), followed by CICN (51\u00a0+\u00a0108; 29\u00a0%) whereas IRN (51\u00a0+\u00a059\u00a0+\u00a0108; 15\u00a0%), CNRNVL (59\u00a0+\u00a0108\u00a0+\u00a0164; 2\u00a0%) and RICN (50\u00a0+\u00a051\u00a0+\u00a0108; 2\u00a0%) triple mutant genotypes were detected. Investigations of the pfdhps gene showed that the 437G mutation was the most prevalent (97\u00a0%). Only two and one samples disclosed the 540E (7\u00a0%) and the 436A (3\u00a0%), respectively. Single mutant SGKAA (437; 86\u00a0%) was higher than SGEAA (437\u00a0+\u00a0540; 7\u00a0%) or AGKAA (436\u00a0+\u00a0437; 3\u00a0%) double mutants genotypes. No polymorphism was detected at codons 581G and 613T/S. Combining pfdhfr and pfdhps alleles two triple mutant haplotypes (double mutant in dhfr and single mutant in dhps) were observed: the ACICNVI/SGKAA in 14 (56\u00a0%) samples and the ACNRNVI/SGKAA in five (20\u00a0%) samples. One quadruple mutant haplotype was detected (ACIRNVI/SGKAA) in six (24\u00a0%) P. falciparum samples. No quintuple pfdhfr-pfdhps mutant was noted.\nCONCLUSION: pfdhfr and pfdhps gene mutations in isolates from Lubango are suggestive of a low-grade SP resistance and IPT for pregnant women and infant based on SP treatment could be effective. Routine molecular studies targeting polymorphism in these two genes need to be routinely conducted at country level.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1186/s12936-016-1358-7", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1030597", 
            "issn": [
              "1475-2875"
            ], 
            "name": "Malaria Journal", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "15"
          }
        ], 
        "name": "Low-grade sulfadoxine\u2013pyrimethamine resistance in Plasmodium falciparum parasites from Lubango, Angola", 
        "pagination": "309", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "8c6ee0c493a11382e5849e62e2ecde694e4c8af735ded109f70987141e3e8e7e"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "27267365"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101139802"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s12936-016-1358-7"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1029478986"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s12936-016-1358-7", 
          "https://app.dimensions.ai/details/publication/pub.1029478986"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:38", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000363_0000000363/records_70043_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1186%2Fs12936-016-1358-7"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12936-016-1358-7'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12936-016-1358-7'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12936-016-1358-7'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12936-016-1358-7'


     

    This table displays all metadata directly associated to this object as RDF triples.

    336 TRIPLES      21 PREDICATES      89 URIs      37 LITERALS      25 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s12936-016-1358-7 schema:about N0f20eeab9a8746768d8ccaf4dd2c752a
    2 N270d0e4a29b9442aa8cafc15ce1245b8
    3 N3ce95a2cf05d4983be0d53facd39e228
    4 N3ea805b6c976495cb1253106c33e4123
    5 N471b2e399c244c0e806e64e0b4dd2702
    6 N558d655b3e3e4774bc1426d103424515
    7 N56635904839744ea81b2f6085af198c5
    8 N5dc2f4c3f1ff4f86a2fc25d1286b5bc3
    9 N642b465af44e45b39f2c757e04a29ae2
    10 N688fef562bf14db1a80aa3605a8fa8ff
    11 N7cbdb3abac984b09a90d1ec50106281d
    12 Nc2613312f9614e838fe95dce3a2b37fc
    13 Ne00f86ba06b94c9aa103058bf89401ce
    14 Ne40b5b7aed6d43618a7cbb05647cbb33
    15 Nf292ca71cd094ba48dbf0542909bf28e
    16 Nfa9a694ad6d34216b8365e76742312c6
    17 anzsrc-for:06
    18 anzsrc-for:0604
    19 schema:author N039beb785cf849189307b1beea330feb
    20 schema:citation sg:pub.10.1007/s004360050173
    21 sg:pub.10.1186/1471-2334-10-163
    22 sg:pub.10.1186/1471-2334-8-105
    23 sg:pub.10.1186/1475-2875-10-22
    24 sg:pub.10.1186/1475-2875-10-248
    25 sg:pub.10.1186/1475-2875-10-385
    26 sg:pub.10.1186/1475-2875-5-54
    27 sg:pub.10.1186/1475-2875-7-236
    28 sg:pub.10.1186/1475-2875-8-89
    29 sg:pub.10.1186/1475-2875-9-247
    30 sg:pub.10.1186/s12936-015-0634-2
    31 https://doi.org/10.1016/0166-6851(94)00207-4
    32 https://doi.org/10.1016/j.actatropica.2006.07.002
    33 https://doi.org/10.1016/j.actatropica.2007.04.006
    34 https://doi.org/10.1016/j.meegid.2009.04.015
    35 https://doi.org/10.1016/j.pt.2013.07.008
    36 https://doi.org/10.1016/j.pt.2013.08.002
    37 https://doi.org/10.1016/s0140-6736(03)12951-0
    38 https://doi.org/10.1073/pnas.87.8.3014
    39 https://doi.org/10.1073/pnas.87.8.3018
    40 https://doi.org/10.1080/00034980120103234
    41 https://doi.org/10.1086/342946
    42 https://doi.org/10.1086/504687
    43 https://doi.org/10.1086/518512
    44 https://doi.org/10.1086/587845
    45 https://doi.org/10.1086/588198
    46 https://doi.org/10.1093/infdis/jiu836
    47 https://doi.org/10.1111/j.1365-3156.2004.01239.x
    48 https://doi.org/10.1111/j.1365-3156.2005.01471.x
    49 https://doi.org/10.1111/j.1365-3156.2007.01904.x
    50 https://doi.org/10.1111/j.1365-3156.2009.02369.x
    51 https://doi.org/10.1111/j.1432-1033.1994.00397.x
    52 https://doi.org/10.1126/science.1098876
    53 https://doi.org/10.1128/aac.00498-09
    54 https://doi.org/10.1128/aac.43.9.2205
    55 https://doi.org/10.1128/aac.47.4.1347-1354.2003
    56 https://doi.org/10.1371/journal.pone.0004364
    57 https://doi.org/10.1371/journal.pone.0012618
    58 https://doi.org/10.1590/s0074-02762000000500020
    59 https://doi.org/10.3201/eid2008.131897
    60 https://doi.org/10.4238/vol7-3x-meeting04
    61 https://doi.org/10.4269/ajtmh.2005.73.1131
    62 https://doi.org/10.4269/ajtmh.2006.74.205
    63 https://doi.org/10.4269/ajtmh.2009.80.523
    64 schema:datePublished 2016-12
    65 schema:datePublishedReg 2016-12-01
    66 schema:description BACKGROUND: Malaria is a major parasitic disease, affecting millions of people in endemic areas. Plasmodium falciparum parasites are responsible for the most severe cases and its resistance to anti-malarial drugs is notorious. This is a possible obstacle to the effectiveness of intermittent preventive treatment (IPT) based on sulfadoxine-pyrimethamine (SP) cures administrated to pregnant women (IPTp) during their pregnancy. As this intervention is recommended in Angola since 2006, it has assessed, in this country, the molecular profiles in P. falciparum dhfr and dhps, two polymorphic genes associated to pyrimethamine and sulfadoxine resistance, respectively. METHODS: Blood samples from 52 falciparum patients were collected in Lubango, Angola and pfdhfr and pfdhps polymorphisms were analysed using nested-PCR and DNA sequencing. RESULTS: In the pfdhfr gene, the 108N mutation was almost fixed (98 %), followed by 59R (63 %), 51I (46 %), 50R and 164L (2 %, respectively). No 16V/S mutations were found. The most common double mutant genotype was CNRN (59 + 108; 46 %), followed by CICN (51 + 108; 29 %) whereas IRN (51 + 59 + 108; 15 %), CNRNVL (59 + 108 + 164; 2 %) and RICN (50 + 51 + 108; 2 %) triple mutant genotypes were detected. Investigations of the pfdhps gene showed that the 437G mutation was the most prevalent (97 %). Only two and one samples disclosed the 540E (7 %) and the 436A (3 %), respectively. Single mutant SGKAA (437; 86 %) was higher than SGEAA (437 + 540; 7 %) or AGKAA (436 + 437; 3 %) double mutants genotypes. No polymorphism was detected at codons 581G and 613T/S. Combining pfdhfr and pfdhps alleles two triple mutant haplotypes (double mutant in dhfr and single mutant in dhps) were observed: the ACICNVI/SGKAA in 14 (56 %) samples and the ACNRNVI/SGKAA in five (20 %) samples. One quadruple mutant haplotype was detected (ACIRNVI/SGKAA) in six (24 %) P. falciparum samples. No quintuple pfdhfr-pfdhps mutant was noted. CONCLUSION: pfdhfr and pfdhps gene mutations in isolates from Lubango are suggestive of a low-grade SP resistance and IPT for pregnant women and infant based on SP treatment could be effective. Routine molecular studies targeting polymorphism in these two genes need to be routinely conducted at country level.
    67 schema:genre research_article
    68 schema:inLanguage en
    69 schema:isAccessibleForFree true
    70 schema:isPartOf N0a1bae2e20ed414289cfa13b8719ab7b
    71 Nb649c30a11ab4bc29583fd62f31a2e8b
    72 sg:journal.1030597
    73 schema:name Low-grade sulfadoxine–pyrimethamine resistance in Plasmodium falciparum parasites from Lubango, Angola
    74 schema:pagination 309
    75 schema:productId N3cb26d3e310f426fb8e54b83ba70de3e
    76 N4e768290e7a6422991fc1568683df776
    77 N67d861b5f2e64891bb3e09a78cf9ffe6
    78 N69770eb17345477fab75b48538a38ca0
    79 Nbab0c9b3a3314ccca90e552d92755b4c
    80 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029478986
    81 https://doi.org/10.1186/s12936-016-1358-7
    82 schema:sdDatePublished 2019-04-11T12:38
    83 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    84 schema:sdPublisher N1f3de3c3de2c4cc3af5645f90964fd40
    85 schema:url https://link.springer.com/10.1186%2Fs12936-016-1358-7
    86 sgo:license sg:explorer/license/
    87 sgo:sdDataset articles
    88 rdf:type schema:ScholarlyArticle
    89 N014cf89bba494c618a7bc82d065a7140 rdf:first sg:person.0755351436.01
    90 rdf:rest Nfdaa0110f81e43038593be50679607bf
    91 N039beb785cf849189307b1beea330feb rdf:first N26a57d035f994c6c85a64d54067148e6
    92 rdf:rest N0c37e4aecd6c4438a941a2e2e402be56
    93 N09ae4c09ee57408fb554af9df15e41bf rdf:first sg:person.010067171043.79
    94 rdf:rest rdf:nil
    95 N0a1bae2e20ed414289cfa13b8719ab7b schema:issueNumber 1
    96 rdf:type schema:PublicationIssue
    97 N0c37e4aecd6c4438a941a2e2e402be56 rdf:first sg:person.01261510365.42
    98 rdf:rest Ndce70b07b23642c7b504764acc644e75
    99 N0f20eeab9a8746768d8ccaf4dd2c752a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Mutation
    101 rdf:type schema:DefinedTerm
    102 N1f3de3c3de2c4cc3af5645f90964fd40 schema:name Springer Nature - SN SciGraph project
    103 rdf:type schema:Organization
    104 N26a57d035f994c6c85a64d54067148e6 schema:affiliation https://www.grid.ac/institutes/grid.418068.3
    105 schema:familyName Kaingona-Daniel
    106 schema:givenName Elsa P. S.
    107 rdf:type schema:Person
    108 N270d0e4a29b9442aa8cafc15ce1245b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    109 schema:name Tetrahydrofolate Dehydrogenase
    110 rdf:type schema:DefinedTerm
    111 N3cb26d3e310f426fb8e54b83ba70de3e schema:name dimensions_id
    112 schema:value pub.1029478986
    113 rdf:type schema:PropertyValue
    114 N3ce95a2cf05d4983be0d53facd39e228 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    115 schema:name Dihydropteroate Synthase
    116 rdf:type schema:DefinedTerm
    117 N3ea805b6c976495cb1253106c33e4123 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name Sulfadoxine
    119 rdf:type schema:DefinedTerm
    120 N471b2e399c244c0e806e64e0b4dd2702 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Plasmodium falciparum
    122 rdf:type schema:DefinedTerm
    123 N4e768290e7a6422991fc1568683df776 schema:name pubmed_id
    124 schema:value 27267365
    125 rdf:type schema:PropertyValue
    126 N558d655b3e3e4774bc1426d103424515 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Polymorphism, Genetic
    128 rdf:type schema:DefinedTerm
    129 N56635904839744ea81b2f6085af198c5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Humans
    131 rdf:type schema:DefinedTerm
    132 N5dc2f4c3f1ff4f86a2fc25d1286b5bc3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Malaria, Falciparum
    134 rdf:type schema:DefinedTerm
    135 N642b465af44e45b39f2c757e04a29ae2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    136 schema:name Antimalarials
    137 rdf:type schema:DefinedTerm
    138 N67d861b5f2e64891bb3e09a78cf9ffe6 schema:name readcube_id
    139 schema:value 8c6ee0c493a11382e5849e62e2ecde694e4c8af735ded109f70987141e3e8e7e
    140 rdf:type schema:PropertyValue
    141 N688fef562bf14db1a80aa3605a8fa8ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    142 schema:name Sequence Analysis, DNA
    143 rdf:type schema:DefinedTerm
    144 N69770eb17345477fab75b48538a38ca0 schema:name doi
    145 schema:value 10.1186/s12936-016-1358-7
    146 rdf:type schema:PropertyValue
    147 N76dc8fa510aa412b983541a8d232b19d rdf:first sg:person.01003711753.89
    148 rdf:rest N014cf89bba494c618a7bc82d065a7140
    149 N7cbdb3abac984b09a90d1ec50106281d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Drug Combinations
    151 rdf:type schema:DefinedTerm
    152 Na7cad0f3251e46359f30de45cc918ebf rdf:first sg:person.01237167267.74
    153 rdf:rest N76dc8fa510aa412b983541a8d232b19d
    154 Nb649c30a11ab4bc29583fd62f31a2e8b schema:volumeNumber 15
    155 rdf:type schema:PublicationVolume
    156 Nbab0c9b3a3314ccca90e552d92755b4c schema:name nlm_unique_id
    157 schema:value 101139802
    158 rdf:type schema:PropertyValue
    159 Nc2613312f9614e838fe95dce3a2b37fc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    160 schema:name Drug Resistance
    161 rdf:type schema:DefinedTerm
    162 Nc96338e27a83484e86baa46dd4778c57 schema:name Angolan National Malaria Control Programme, National Institute of Public Health, Luanda, Angola
    163 Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDESMal/CPLP), Lisbon, Portugal
    164 rdf:type schema:Organization
    165 Ndce70b07b23642c7b504764acc644e75 rdf:first sg:person.01307357647.97
    166 rdf:rest Na7cad0f3251e46359f30de45cc918ebf
    167 Ne00f86ba06b94c9aa103058bf89401ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    168 schema:name Angola
    169 rdf:type schema:DefinedTerm
    170 Ne40b5b7aed6d43618a7cbb05647cbb33 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    171 schema:name Protozoan Proteins
    172 rdf:type schema:DefinedTerm
    173 Nf292ca71cd094ba48dbf0542909bf28e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    174 schema:name Polymerase Chain Reaction
    175 rdf:type schema:DefinedTerm
    176 Nfa9a694ad6d34216b8365e76742312c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    177 schema:name Pyrimethamine
    178 rdf:type schema:DefinedTerm
    179 Nfdaa0110f81e43038593be50679607bf rdf:first sg:person.01220713203.83
    180 rdf:rest N09ae4c09ee57408fb554af9df15e41bf
    181 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    182 schema:name Biological Sciences
    183 rdf:type schema:DefinedTerm
    184 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    185 schema:name Genetics
    186 rdf:type schema:DefinedTerm
    187 sg:journal.1030597 schema:issn 1475-2875
    188 schema:name Malaria Journal
    189 rdf:type schema:Periodical
    190 sg:person.01003711753.89 schema:affiliation Nc96338e27a83484e86baa46dd4778c57
    191 schema:familyName Fortes
    192 schema:givenName Filomeno
    193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003711753.89
    194 rdf:type schema:Person
    195 sg:person.010067171043.79 schema:affiliation https://www.grid.ac/institutes/grid.418068.3
    196 schema:familyName Ferreira-da-Cruz
    197 schema:givenName Maria de Fátima
    198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010067171043.79
    199 rdf:type schema:Person
    200 sg:person.01220713203.83 schema:affiliation https://www.grid.ac/institutes/grid.418068.3
    201 schema:familyName Daniel-Ribeiro
    202 schema:givenName Cláudio Tadeu
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220713203.83
    204 rdf:type schema:Person
    205 sg:person.01237167267.74 schema:affiliation https://www.grid.ac/institutes/grid.418068.3
    206 schema:familyName Almeida-de-Oliveira
    207 schema:givenName Natália K.
    208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237167267.74
    209 rdf:type schema:Person
    210 sg:person.01261510365.42 schema:affiliation https://www.grid.ac/institutes/grid.418068.3
    211 schema:familyName Gomes
    212 schema:givenName Larissa Rodrigues
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261510365.42
    214 rdf:type schema:Person
    215 sg:person.01307357647.97 schema:affiliation https://www.grid.ac/institutes/grid.419166.d
    216 schema:familyName Gama
    217 schema:givenName Bianca E.
    218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307357647.97
    219 rdf:type schema:Person
    220 sg:person.0755351436.01 schema:affiliation https://www.grid.ac/institutes/grid.418537.c
    221 schema:familyName Ménard
    222 schema:givenName Didier
    223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755351436.01
    224 rdf:type schema:Person
    225 sg:pub.10.1007/s004360050173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040895283
    226 https://doi.org/10.1007/s004360050173
    227 rdf:type schema:CreativeWork
    228 sg:pub.10.1186/1471-2334-10-163 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051632152
    229 https://doi.org/10.1186/1471-2334-10-163
    230 rdf:type schema:CreativeWork
    231 sg:pub.10.1186/1471-2334-8-105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049429367
    232 https://doi.org/10.1186/1471-2334-8-105
    233 rdf:type schema:CreativeWork
    234 sg:pub.10.1186/1475-2875-10-22 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026962984
    235 https://doi.org/10.1186/1475-2875-10-22
    236 rdf:type schema:CreativeWork
    237 sg:pub.10.1186/1475-2875-10-248 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004333404
    238 https://doi.org/10.1186/1475-2875-10-248
    239 rdf:type schema:CreativeWork
    240 sg:pub.10.1186/1475-2875-10-385 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050786193
    241 https://doi.org/10.1186/1475-2875-10-385
    242 rdf:type schema:CreativeWork
    243 sg:pub.10.1186/1475-2875-5-54 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030186746
    244 https://doi.org/10.1186/1475-2875-5-54
    245 rdf:type schema:CreativeWork
    246 sg:pub.10.1186/1475-2875-7-236 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010316876
    247 https://doi.org/10.1186/1475-2875-7-236
    248 rdf:type schema:CreativeWork
    249 sg:pub.10.1186/1475-2875-8-89 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041383647
    250 https://doi.org/10.1186/1475-2875-8-89
    251 rdf:type schema:CreativeWork
    252 sg:pub.10.1186/1475-2875-9-247 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004545394
    253 https://doi.org/10.1186/1475-2875-9-247
    254 rdf:type schema:CreativeWork
    255 sg:pub.10.1186/s12936-015-0634-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016316151
    256 https://doi.org/10.1186/s12936-015-0634-2
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1016/0166-6851(94)00207-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042312772
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1016/j.actatropica.2006.07.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037857315
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1016/j.actatropica.2007.04.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031371734
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1016/j.meegid.2009.04.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049881547
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1016/j.pt.2013.07.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006679019
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1016/j.pt.2013.08.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037644778
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1016/s0140-6736(03)12951-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018879851
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1073/pnas.87.8.3014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046332862
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1073/pnas.87.8.3018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018549017
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1080/00034980120103234 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058260561
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.1086/342946 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029635400
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1086/504687 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002637513
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1086/518512 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013987624
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1086/587845 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007387705
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1086/588198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032103986
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1093/infdis/jiu836 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042920146
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1111/j.1365-3156.2004.01239.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019298701
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1111/j.1365-3156.2005.01471.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044901893
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1111/j.1365-3156.2007.01904.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023497284
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1111/j.1365-3156.2009.02369.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044331506
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1111/j.1432-1033.1994.00397.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051593980
    299 rdf:type schema:CreativeWork
    300 https://doi.org/10.1126/science.1098876 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062449701
    301 rdf:type schema:CreativeWork
    302 https://doi.org/10.1128/aac.00498-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037804573
    303 rdf:type schema:CreativeWork
    304 https://doi.org/10.1128/aac.43.9.2205 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074515067
    305 rdf:type schema:CreativeWork
    306 https://doi.org/10.1128/aac.47.4.1347-1354.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040132193
    307 rdf:type schema:CreativeWork
    308 https://doi.org/10.1371/journal.pone.0004364 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016260428
    309 rdf:type schema:CreativeWork
    310 https://doi.org/10.1371/journal.pone.0012618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038898955
    311 rdf:type schema:CreativeWork
    312 https://doi.org/10.1590/s0074-02762000000500020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024617218
    313 rdf:type schema:CreativeWork
    314 https://doi.org/10.3201/eid2008.131897 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016346861
    315 rdf:type schema:CreativeWork
    316 https://doi.org/10.4238/vol7-3x-meeting04 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072396808
    317 rdf:type schema:CreativeWork
    318 https://doi.org/10.4269/ajtmh.2005.73.1131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077161643
    319 rdf:type schema:CreativeWork
    320 https://doi.org/10.4269/ajtmh.2006.74.205 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077188629
    321 rdf:type schema:CreativeWork
    322 https://doi.org/10.4269/ajtmh.2009.80.523 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077882214
    323 rdf:type schema:CreativeWork
    324 https://www.grid.ac/institutes/grid.418068.3 schema:alternateName Oswaldo Cruz Foundation
    325 schema:name Centro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal) Fiocruz, Rio de Janeiro, Brazil
    326 Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDESMal/CPLP), Lisbon, Portugal
    327 Hospital Central Dr. António Agostinho Neto, Lubango, Angola
    328 Laboratório de Pesquisa em Malária—Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil
    329 rdf:type schema:Organization
    330 https://www.grid.ac/institutes/grid.418537.c schema:alternateName Institut Pasteur du Cambodge
    331 schema:name Malaria Molecular Epidemiology Unit, Institut Pasteur in Cambodia, Phnom Penh, Cambodia
    332 rdf:type schema:Organization
    333 https://www.grid.ac/institutes/grid.419166.d schema:alternateName Instituto Nacional do Câncer
    334 schema:name Laboratory of Oncovirology, Instituto Nacional de Câncer, Rio de Janeiro, Brazil
    335 Laboratório de Pesquisa em Malária—Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil
    336 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...