Equilibrium of the intracellular redox state for improving cell growth and L-lysine yield of Corynebacterium glutamicum by optimal cofactor swapping. View Full Text


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Article Info

DATE

2019-12

AUTHORS

Jian-Zhong Xu, Hao-Zhe Ruan, Xiu-Lai Chen, Feng Zhang, Weiguo Zhang

ABSTRACT

BACKGROUND: NAD(H/+) and NADP(H/+) are the most important redox cofactors in bacteria. However, the intracellular redox balance is in advantage of the cell growth and production of NAD(P)H-dependent products. RESULTS: In this paper, we rationally engineered glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and isocitrate dehydrogenase (IDH) to switch the nucleotide-cofactor specificity resulting in an increase in final titer [from 85.6 to 121.4 g L-1] and carbon yield [from 0.33 to 0.46 g (g glucose)-1] of L-lysine in strain RGI in fed-batch fermentation. To do this, we firstly analyzed the production performance of original strain JL-6, indicating that the imbalance of intracellular redox was the limiting factor for L-lysine production. Subsequently, we modified the native GAPDH and indicated that recombinant strain RG with nonnative NADP-GAPDH dramatically changed the intracellular levels of NADH and NADPH. However, L-lysine production did not significantly increase because cell growth was harmed at low NADH level. Lastly, the nonnative NAD-IDH was introduced in strain RG to increase the NADH availability and to equilibrate the intracellular redox. The resulted strain RGI showed the stable ratio of NADPH/NADH at about 1.00, which in turn improved cell growth (μmax. = 0.31 h-1) and L-lysine productivity (qLys, max. = 0.53 g g-1 h-1) as compared with strain RG (μmax. = 0.14 h-1 and qLys, max. = 0.42 g g-1 h-1). CONCLUSIONS: This is the first report of balancing the intracellular redox state by switching the nucleotide-cofactor specificity of GAPDH and IDH, thereby improving cell growth and L-lysine production. More... »

PAGES

65

References to SciGraph publications

  • 1999-10. A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2007-10. Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum in MICROBIAL CELL FACTORIES
  • 2015-08. Novel Type II and Monomeric NAD+ Specific Isocitrate Dehydrogenases: Phylogenetic Affinity, Enzymatic Characterization, and Evolutionary Implication in SCIENTIFIC REPORTS
  • 2017-02. Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox metabolism in NATURE BIOTECHNOLOGY
  • 2018-12. Rational modification of tricarboxylic acid cycle for improving l-lysine production in Corynebacterium glutamicum in MICROBIAL CELL FACTORIES
  • 2014-09. Metabolic engineering Corynebacterium glutamicum for the l-lysine production by increasing the flux into l-lysine biosynthetic pathway in AMINO ACIDS
  • 2013-10. Metabolic engineering of Corynebacterium glutamicum for increasing the production of l-ornithine by increasing NADPH availability in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2018-03. Engineering Escherichia coli for glycolic acid production from D-xylose through the Dahms pathway and glyoxylate bypass in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2013-12. Improvement of cell growth and l-lysine production by genetically modified Corynebacterium glutamicum during growth on molasses in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2016-12. Effect of Polyhydroxybutyrate (PHB) storage on l-arginine production in recombinant Corynebacterium crenatum using coenzyme regulation in MICROBIAL CELL FACTORIES
  • 2012-11. Multifunctional Roles of NAD+ and NADH in Astrocytes in NEUROCHEMICAL RESEARCH
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    DOI

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    45 schema:description BACKGROUND: NAD(H/+) and NADP(H/+) are the most important redox cofactors in bacteria. However, the intracellular redox balance is in advantage of the cell growth and production of NAD(P)H-dependent products. RESULTS: In this paper, we rationally engineered glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and isocitrate dehydrogenase (IDH) to switch the nucleotide-cofactor specificity resulting in an increase in final titer [from 85.6 to 121.4 g L-1] and carbon yield [from 0.33 to 0.46 g (g glucose)-1] of L-lysine in strain RGI in fed-batch fermentation. To do this, we firstly analyzed the production performance of original strain JL-6, indicating that the imbalance of intracellular redox was the limiting factor for L-lysine production. Subsequently, we modified the native GAPDH and indicated that recombinant strain RG with nonnative NADP-GAPDH dramatically changed the intracellular levels of NADH and NADPH. However, L-lysine production did not significantly increase because cell growth was harmed at low NADH level. Lastly, the nonnative NAD-IDH was introduced in strain RG to increase the NADH availability and to equilibrate the intracellular redox. The resulted strain RGI showed the stable ratio of NADPH/NADH at about 1.00, which in turn improved cell growth (μmax. = 0.31 h-1) and L-lysine productivity (qLys, max. = 0.53 g g-1 h-1) as compared with strain RG (μmax. = 0.14 h-1 and qLys, max. = 0.42 g g-1 h-1). CONCLUSIONS: This is the first report of balancing the intracellular redox state by switching the nucleotide-cofactor specificity of GAPDH and IDH, thereby improving cell growth and L-lysine production.
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