Construction of DGLA producing cell factory by genetic modification of Mucor circinelloides. View Full Text


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Article Info

DATE

2019-12

AUTHORS

Md Ahsanul Kabir Khan, Junhuan Yang, Syed Ammar Hussain, Huaiyuan Zhang, Li Liang, Victoriano Garre, Yuanda Song

ABSTRACT

BACKGROUND: Dihomo-gamma linolenic acid (DGLA, 20:3, n-6) is the elongated product of Gamma linolenic acid (GLA, 18:3, n-6) catalyzed by the enzyme delta-6 elongase (D6E) or gamma linolenic acid elongase (GLELO). Construction of engineered oleaginous microbes have been attracting significant interest to produce DGLA because of its nutritional value and medicinal applications. Mucor circinelloides is a GLA producing filamentous fungus which can be a useful tool to produce DGLA. We have, therefore, overexpressed the D6E (GLELO) gene in this fungus to construct DGLA producing cell factory. RESULT: To produce DGLA in M. circinelloides, homologous overexpression of D6E (GLELO) gene was analyzed. When the gene was overexpressed in M. circinelloides CBS277.49, up to 5.72% DGLA was produced in this strain. CONCLUSION: To our knowledge, this is the first report describing the overexpression of D6E (GLELO) gene in M. circinelloides to construct DGLA producing cell factory. A new scope for further research has been established by this work for improved production of DGLA in this fungus, specifically in its high lipid-producing strain, WJ11. More... »

PAGES

64

References to SciGraph publications

  • 2008-12. Functional analysis of a fatty acid elongase from arachidonic acid-producing Mortierella alpina 1S-4 in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2015-09. Identification of a critical determinant that enables efficient fatty acid synthesis in oleaginous fungi in SCIENTIFIC REPORTS
  • 2012-12. Multiple roles of dihomo-γ-linolenic acid against proliferation diseases in LIPIDS IN HEALTH AND DISEASE
  • 2005-01. Molecular evidence that the rate-limiting step for the biosynthesis of arachidonic acid in Mortierella alpina is at the level of an elongase in LIPIDS
  • 2008-12. Essential fatty acids and their metabolites could function as endogenous HMG-CoA reductase and ACE enzyme inhibitors, anti-arrhythmic, anti-hypertensive, anti-atherosclerotic, anti-inflammatory, cytoprotective, and cardioprotective molecules in LIPIDS IN HEALTH AND DISEASE
  • 2013-04. Malic enzyme activity is not the only bottleneck for lipid accumulation in the oleaginous fungus Mucor circinelloides in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2016-12. Proteomics analysis of high lipid-producing strain Mucor circinelloides WJ11: an explanation for the mechanism of lipid accumulation at the proteomic level in MICROBIAL CELL FACTORIES
  • 2017-12. Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases in MICROBIAL CELL FACTORIES
  • 2014-02. Proteomics to reveal metabolic network shifts towards lipid accumulation following nitrogen deprivation in the diatom Phaeodactylum tricornutum in JOURNAL OF APPLIED PHYCOLOGY
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    http://scigraph.springernature.com/pub.10.1186/s12934-019-1110-4

    DOI

    http://dx.doi.org/10.1186/s12934-019-1110-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1113181809

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30943965


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    37 schema:description BACKGROUND: Dihomo-gamma linolenic acid (DGLA, 20:3, n-6) is the elongated product of Gamma linolenic acid (GLA, 18:3, n-6) catalyzed by the enzyme delta-6 elongase (D6E) or gamma linolenic acid elongase (GLELO). Construction of engineered oleaginous microbes have been attracting significant interest to produce DGLA because of its nutritional value and medicinal applications. Mucor circinelloides is a GLA producing filamentous fungus which can be a useful tool to produce DGLA. We have, therefore, overexpressed the D6E (GLELO) gene in this fungus to construct DGLA producing cell factory. RESULT: To produce DGLA in M. circinelloides, homologous overexpression of D6E (GLELO) gene was analyzed. When the gene was overexpressed in M. circinelloides CBS277.49, up to 5.72% DGLA was produced in this strain. CONCLUSION: To our knowledge, this is the first report describing the overexpression of D6E (GLELO) gene in M. circinelloides to construct DGLA producing cell factory. A new scope for further research has been established by this work for improved production of DGLA in this fungus, specifically in its high lipid-producing strain, WJ11.
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