Large-scale production of tauroursodeoxycholic acid products through fermentation optimization of engineered Escherichia coli cell factory View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Yingpeng Xu, Li Yang, Shujuan Zhao, Zhengtao Wang

ABSTRACT

BACKGROUND: Bear bile powder is a valuable medicinal material characterized by high content of tauroursodeoxycholic acid (TUDCA) at a certain ratio to taurochenodeoxycholic acid (TCDCA). We had created an engineered E. coli harboring two-step bidirectional oxidative and reductive enzyme-catalyzing pathway that could rapidly convert TCDCA to TUDCA at a specific percentage in shake flasks. RESULTS: We reported here the large-scale production of TUDCA containing products by balancing the bidirectional reactions through optimizing fermentation process of the engineered E. coli in fermenters. The fermentation medium was firstly optimized based on M9 medium using response surface methodology, leading to a glycerol and yeast extract modified M9-GY medium benefits for both cell growth and product conversion efficiency. Then isopropylthio-β-galactoside induction and fed-stock stage was successively optimized. Finally, a special deep-tank static process was developed to promote the conversion from TCDCA to TUDCA. Applying the optimal condition, fermentation was performed by separately supplementing 30 g refined chicken bile powder and 35 g crude chicken bile powder as substrates, resulting in 29.35 ± 2.83 g and 30.78 ± 3.04 g powder products containing 35.85 ± 3.85% and 27.14 ± 4.23% of TUDCA at a ratio of 1.49 ± 0.14 and 1.55 ± 0.19 to TCDCA, respectively, after purification and evaporation of the fermentation broth. The recovery yield was 92.84 ± 4.21% and 91.83 ± 2.56%, respectively. CONCLUSION: This study provided a practical and environment friendly industrialized process for producing artificial substitute of bear bile powder from cheap and readily available chicken bile powder using engineered E. coli microbial cell factory. It also put forward an interesting deep-tank static process to promote the enzyme-catalyzing reactions toward target compounds in synthetic biology-based fermentation. More... »

PAGES

34

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12934-019-1076-2

DOI

http://dx.doi.org/10.1186/s12934-019-1076-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112023611

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30736766


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Shanghai University of Traditional Chinese Medicine", 
          "id": "https://www.grid.ac/institutes/grid.412540.6", 
          "name": [
            "The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xu", 
        "givenName": "Yingpeng", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shanghai University of Traditional Chinese Medicine", 
          "id": "https://www.grid.ac/institutes/grid.412540.6", 
          "name": [
            "The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Li", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shanghai University of Traditional Chinese Medicine", 
          "id": "https://www.grid.ac/institutes/grid.412540.6", 
          "name": [
            "The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhao", 
        "givenName": "Shujuan", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shanghai University of Traditional Chinese Medicine", 
          "id": "https://www.grid.ac/institutes/grid.412540.6", 
          "name": [
            "The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Zhengtao", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s00253-007-1227-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000914907", 
          "https://doi.org/10.1007/s00253-007-1227-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-007-1227-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000914907", 
          "https://doi.org/10.1007/s00253-007-1227-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1746-4269-5-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001230731", 
          "https://doi.org/10.1186/1746-4269-5-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s13197-014-1570-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002131177", 
          "https://doi.org/10.1007/s13197-014-1570-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11745-997-0099-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005089007", 
          "https://doi.org/10.1007/s11745-997-0099-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00018-008-7568-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005438904", 
          "https://doi.org/10.1007/s00018-008-7568-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.3095", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006577547", 
          "https://doi.org/10.1038/nbt.3095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12035-016-0145-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009471950", 
          "https://doi.org/10.1007/s12035-016-0145-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12035-016-0145-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009471950", 
          "https://doi.org/10.1007/s12035-016-0145-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bab.1378", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009569561"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1194/jlr.m039834", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010131388"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcatb.2010.12.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011919872"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2859-10-39", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013390138", 
          "https://doi.org/10.1186/1475-2859-10-39"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1631/jzus.b0820038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014461114", 
          "https://doi.org/10.1631/jzus.b0820038"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbiotec.2014.04.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020049009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2016.06.127", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021196096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1557", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022462669", 
          "https://doi.org/10.1038/nbt.1557"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1557", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022462669", 
          "https://doi.org/10.1038/nbt.1557"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mmbr.00025-07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022536129"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-012-4340-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025194941", 
          "https://doi.org/10.1007/s00253-012-4340-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10295-016-1856-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027744197", 
          "https://doi.org/10.1007/s10295-016-1856-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10295-016-1856-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027744197", 
          "https://doi.org/10.1007/s10295-016-1856-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-011-3798-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031588254", 
          "https://doi.org/10.1007/s00253-011-3798-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c3ay41605d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031702810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bmc.2012.01.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031706812"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.copbio.2014.11.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031753111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.copbio.2014.11.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031753111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.copbio.2014.11.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031753111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.copbio.2014.11.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031753111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cmdc.201300059", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034008777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-006-0554-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036673723", 
          "https://doi.org/10.1007/s00253-006-0554-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-006-0554-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036673723", 
          "https://doi.org/10.1007/s00253-006-0554-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2014.01.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037630230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.procbio.2014.12.026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037850350"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.24547", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038665792"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2016/4305074", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039021937"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymben.2016.01.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039476466"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jchromb.2014.09.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047224904"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2009.03.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047853415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep11301", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048371555", 
          "https://doi.org/10.1038/srep11301"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-012-4072-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049072089", 
          "https://doi.org/10.1007/s00253-012-4072-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymben.2014.12.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052909138"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2859-10-104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053595996", 
          "https://doi.org/10.1186/1475-2859-10-104"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/tx800225q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056299890"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/tx800225q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056299890"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081950181", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/03639045.2017.1402922", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092578204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-018-21621-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101008565", 
          "https://doi.org/10.1038/s41598-018-21621-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.19540/j.cnki.cjcmm.20180125.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105155413"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.19540/j.cnki.cjcmm.20180125.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105155413"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biotechadv.2018.07.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105704604"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "BACKGROUND: Bear bile powder is a valuable medicinal material characterized by high content of tauroursodeoxycholic acid (TUDCA) at a certain ratio to taurochenodeoxycholic acid (TCDCA). We had created an engineered E. coli harboring two-step bidirectional oxidative and reductive enzyme-catalyzing pathway that could rapidly convert TCDCA to TUDCA at a specific percentage in shake flasks.\nRESULTS: We reported here the large-scale production of TUDCA containing products by balancing the bidirectional reactions through optimizing fermentation process of the engineered E. coli in fermenters. The fermentation medium was firstly optimized based on M9 medium using response surface methodology, leading to a glycerol and yeast extract modified M9-GY medium benefits for both cell growth and product conversion efficiency. Then isopropylthio-\u03b2-galactoside induction and fed-stock stage was successively optimized. Finally, a special deep-tank static process was developed to promote the conversion from TCDCA to TUDCA. Applying the optimal condition, fermentation was performed by separately supplementing 30\u00a0g refined chicken bile powder and 35\u00a0g crude chicken bile powder as substrates, resulting in 29.35\u2009\u00b1\u20092.83\u00a0g and 30.78\u2009\u00b1\u20093.04\u00a0g powder products containing 35.85\u2009\u00b1\u20093.85% and 27.14\u2009\u00b1\u20094.23% of TUDCA at a ratio of 1.49\u2009\u00b1\u20090.14 and 1.55\u2009\u00b1\u20090.19 to TCDCA, respectively, after purification and evaporation of the fermentation broth. The recovery yield was 92.84\u2009\u00b1\u20094.21% and 91.83\u2009\u00b1\u20092.56%, respectively.\nCONCLUSION: This study provided a practical and environment friendly industrialized process for producing artificial substitute of bear bile powder from cheap and readily available chicken bile powder using engineered E. coli microbial cell factory. It also put forward an interesting deep-tank static process to promote the enzyme-catalyzing reactions toward target compounds in synthetic biology-based fermentation.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s12934-019-1076-2", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1030599", 
        "issn": [
          "1475-2859"
        ], 
        "name": "Microbial Cell Factories", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "18"
      }
    ], 
    "name": "Large-scale production of tauroursodeoxycholic acid products through fermentation optimization of engineered Escherichia coli cell factory", 
    "pagination": "34", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "9a48ecbeed4e6a88b68c5232b3037879011155f7aeec2007ac5e02c3109e0405"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30736766"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101139812"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12934-019-1076-2"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112023611"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12934-019-1076-2", 
      "https://app.dimensions.ai/details/publication/pub.1112023611"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:13", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000338_0000000338/records_47989_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs12934-019-1076-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12934-019-1076-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12934-019-1076-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12934-019-1076-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12934-019-1076-2'


 

This table displays all metadata directly associated to this object as RDF triples.

225 TRIPLES      21 PREDICATES      70 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12934-019-1076-2 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N1d8b52c97dd14e50b6e7113e68e9a804
4 schema:citation sg:pub.10.1007/s00018-008-7568-6
5 sg:pub.10.1007/s00253-006-0554-8
6 sg:pub.10.1007/s00253-007-1227-y
7 sg:pub.10.1007/s00253-011-3798-x
8 sg:pub.10.1007/s00253-012-4072-6
9 sg:pub.10.1007/s00253-012-4340-5
10 sg:pub.10.1007/s10295-016-1856-1
11 sg:pub.10.1007/s11745-997-0099-8
12 sg:pub.10.1007/s12035-016-0145-3
13 sg:pub.10.1007/s13197-014-1570-9
14 sg:pub.10.1038/nbt.1557
15 sg:pub.10.1038/nbt.3095
16 sg:pub.10.1038/s41598-018-21621-5
17 sg:pub.10.1038/srep11301
18 sg:pub.10.1186/1475-2859-10-104
19 sg:pub.10.1186/1475-2859-10-39
20 sg:pub.10.1186/1746-4269-5-2
21 sg:pub.10.1631/jzus.b0820038
22 https://app.dimensions.ai/details/publication/pub.1081950181
23 https://doi.org/10.1002/bab.1378
24 https://doi.org/10.1002/bit.24547
25 https://doi.org/10.1002/cmdc.201300059
26 https://doi.org/10.1016/j.biortech.2009.03.013
27 https://doi.org/10.1016/j.biortech.2014.01.021
28 https://doi.org/10.1016/j.biortech.2016.06.127
29 https://doi.org/10.1016/j.biotechadv.2018.07.006
30 https://doi.org/10.1016/j.bmc.2012.01.002
31 https://doi.org/10.1016/j.copbio.2014.11.013
32 https://doi.org/10.1016/j.jbiotec.2014.04.025
33 https://doi.org/10.1016/j.jchromb.2014.09.028
34 https://doi.org/10.1016/j.molcatb.2010.12.012
35 https://doi.org/10.1016/j.procbio.2014.12.026
36 https://doi.org/10.1016/j.ymben.2014.12.002
37 https://doi.org/10.1016/j.ymben.2016.01.006
38 https://doi.org/10.1021/tx800225q
39 https://doi.org/10.1039/c3ay41605d
40 https://doi.org/10.1080/03639045.2017.1402922
41 https://doi.org/10.1128/mmbr.00025-07
42 https://doi.org/10.1155/2016/4305074
43 https://doi.org/10.1194/jlr.m039834
44 https://doi.org/10.19540/j.cnki.cjcmm.20180125.001
45 schema:datePublished 2019-12
46 schema:datePublishedReg 2019-12-01
47 schema:description BACKGROUND: Bear bile powder is a valuable medicinal material characterized by high content of tauroursodeoxycholic acid (TUDCA) at a certain ratio to taurochenodeoxycholic acid (TCDCA). We had created an engineered E. coli harboring two-step bidirectional oxidative and reductive enzyme-catalyzing pathway that could rapidly convert TCDCA to TUDCA at a specific percentage in shake flasks. RESULTS: We reported here the large-scale production of TUDCA containing products by balancing the bidirectional reactions through optimizing fermentation process of the engineered E. coli in fermenters. The fermentation medium was firstly optimized based on M9 medium using response surface methodology, leading to a glycerol and yeast extract modified M9-GY medium benefits for both cell growth and product conversion efficiency. Then isopropylthio-β-galactoside induction and fed-stock stage was successively optimized. Finally, a special deep-tank static process was developed to promote the conversion from TCDCA to TUDCA. Applying the optimal condition, fermentation was performed by separately supplementing 30 g refined chicken bile powder and 35 g crude chicken bile powder as substrates, resulting in 29.35 ± 2.83 g and 30.78 ± 3.04 g powder products containing 35.85 ± 3.85% and 27.14 ± 4.23% of TUDCA at a ratio of 1.49 ± 0.14 and 1.55 ± 0.19 to TCDCA, respectively, after purification and evaporation of the fermentation broth. The recovery yield was 92.84 ± 4.21% and 91.83 ± 2.56%, respectively. CONCLUSION: This study provided a practical and environment friendly industrialized process for producing artificial substitute of bear bile powder from cheap and readily available chicken bile powder using engineered E. coli microbial cell factory. It also put forward an interesting deep-tank static process to promote the enzyme-catalyzing reactions toward target compounds in synthetic biology-based fermentation.
48 schema:genre research_article
49 schema:inLanguage en
50 schema:isAccessibleForFree true
51 schema:isPartOf N16199e289c0e40aca5e1bf6175cbd59c
52 N697d35f3f7bd40f2939763aab83b24b2
53 sg:journal.1030599
54 schema:name Large-scale production of tauroursodeoxycholic acid products through fermentation optimization of engineered Escherichia coli cell factory
55 schema:pagination 34
56 schema:productId N201850e1d2f64bb7a63071ab582f03c0
57 N6c0eb7717ce4438aa4cb6a9704f62615
58 N92cc992f7d6743e1a75cfca5577df0ce
59 Ndaa86c3518984d018bec3838d2a4c895
60 Nfc44713a7ab4479c8d7a78cddb9a2718
61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112023611
62 https://doi.org/10.1186/s12934-019-1076-2
63 schema:sdDatePublished 2019-04-11T09:13
64 schema:sdLicense https://scigraph.springernature.com/explorer/license/
65 schema:sdPublisher N9c28aed6e00d458f96d3c60e57e93d41
66 schema:url https://link.springer.com/10.1186%2Fs12934-019-1076-2
67 sgo:license sg:explorer/license/
68 sgo:sdDataset articles
69 rdf:type schema:ScholarlyArticle
70 N16199e289c0e40aca5e1bf6175cbd59c schema:issueNumber 1
71 rdf:type schema:PublicationIssue
72 N1d8b52c97dd14e50b6e7113e68e9a804 rdf:first Nf0a048cee0724aac886225f24e44b424
73 rdf:rest N8513493635b84a7c8ffc80309731a2f3
74 N201850e1d2f64bb7a63071ab582f03c0 schema:name dimensions_id
75 schema:value pub.1112023611
76 rdf:type schema:PropertyValue
77 N41c6d59203a049679bb70a0e5acbe773 rdf:first Na25515fe18414b9cb0bcb649f558d00b
78 rdf:rest rdf:nil
79 N510b23594c7d49bb9e5f0e628c65c227 schema:affiliation https://www.grid.ac/institutes/grid.412540.6
80 schema:familyName Zhao
81 schema:givenName Shujuan
82 rdf:type schema:Person
83 N61c8c02624034dbe9159b880fe222cf2 schema:affiliation https://www.grid.ac/institutes/grid.412540.6
84 schema:familyName Yang
85 schema:givenName Li
86 rdf:type schema:Person
87 N697d35f3f7bd40f2939763aab83b24b2 schema:volumeNumber 18
88 rdf:type schema:PublicationVolume
89 N6c0eb7717ce4438aa4cb6a9704f62615 schema:name readcube_id
90 schema:value 9a48ecbeed4e6a88b68c5232b3037879011155f7aeec2007ac5e02c3109e0405
91 rdf:type schema:PropertyValue
92 N8513493635b84a7c8ffc80309731a2f3 rdf:first N61c8c02624034dbe9159b880fe222cf2
93 rdf:rest Nd84820b4002b42e39605ba12d704b9ea
94 N92cc992f7d6743e1a75cfca5577df0ce schema:name nlm_unique_id
95 schema:value 101139812
96 rdf:type schema:PropertyValue
97 N9c28aed6e00d458f96d3c60e57e93d41 schema:name Springer Nature - SN SciGraph project
98 rdf:type schema:Organization
99 Na25515fe18414b9cb0bcb649f558d00b schema:affiliation https://www.grid.ac/institutes/grid.412540.6
100 schema:familyName Wang
101 schema:givenName Zhengtao
102 rdf:type schema:Person
103 Nd84820b4002b42e39605ba12d704b9ea rdf:first N510b23594c7d49bb9e5f0e628c65c227
104 rdf:rest N41c6d59203a049679bb70a0e5acbe773
105 Ndaa86c3518984d018bec3838d2a4c895 schema:name doi
106 schema:value 10.1186/s12934-019-1076-2
107 rdf:type schema:PropertyValue
108 Nf0a048cee0724aac886225f24e44b424 schema:affiliation https://www.grid.ac/institutes/grid.412540.6
109 schema:familyName Xu
110 schema:givenName Yingpeng
111 rdf:type schema:Person
112 Nfc44713a7ab4479c8d7a78cddb9a2718 schema:name pubmed_id
113 schema:value 30736766
114 rdf:type schema:PropertyValue
115 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
116 schema:name Biological Sciences
117 rdf:type schema:DefinedTerm
118 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
119 schema:name Biochemistry and Cell Biology
120 rdf:type schema:DefinedTerm
121 sg:journal.1030599 schema:issn 1475-2859
122 schema:name Microbial Cell Factories
123 rdf:type schema:Periodical
124 sg:pub.10.1007/s00018-008-7568-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005438904
125 https://doi.org/10.1007/s00018-008-7568-6
126 rdf:type schema:CreativeWork
127 sg:pub.10.1007/s00253-006-0554-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036673723
128 https://doi.org/10.1007/s00253-006-0554-8
129 rdf:type schema:CreativeWork
130 sg:pub.10.1007/s00253-007-1227-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1000914907
131 https://doi.org/10.1007/s00253-007-1227-y
132 rdf:type schema:CreativeWork
133 sg:pub.10.1007/s00253-011-3798-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1031588254
134 https://doi.org/10.1007/s00253-011-3798-x
135 rdf:type schema:CreativeWork
136 sg:pub.10.1007/s00253-012-4072-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049072089
137 https://doi.org/10.1007/s00253-012-4072-6
138 rdf:type schema:CreativeWork
139 sg:pub.10.1007/s00253-012-4340-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025194941
140 https://doi.org/10.1007/s00253-012-4340-5
141 rdf:type schema:CreativeWork
142 sg:pub.10.1007/s10295-016-1856-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027744197
143 https://doi.org/10.1007/s10295-016-1856-1
144 rdf:type schema:CreativeWork
145 sg:pub.10.1007/s11745-997-0099-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005089007
146 https://doi.org/10.1007/s11745-997-0099-8
147 rdf:type schema:CreativeWork
148 sg:pub.10.1007/s12035-016-0145-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009471950
149 https://doi.org/10.1007/s12035-016-0145-3
150 rdf:type schema:CreativeWork
151 sg:pub.10.1007/s13197-014-1570-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002131177
152 https://doi.org/10.1007/s13197-014-1570-9
153 rdf:type schema:CreativeWork
154 sg:pub.10.1038/nbt.1557 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022462669
155 https://doi.org/10.1038/nbt.1557
156 rdf:type schema:CreativeWork
157 sg:pub.10.1038/nbt.3095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006577547
158 https://doi.org/10.1038/nbt.3095
159 rdf:type schema:CreativeWork
160 sg:pub.10.1038/s41598-018-21621-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101008565
161 https://doi.org/10.1038/s41598-018-21621-5
162 rdf:type schema:CreativeWork
163 sg:pub.10.1038/srep11301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048371555
164 https://doi.org/10.1038/srep11301
165 rdf:type schema:CreativeWork
166 sg:pub.10.1186/1475-2859-10-104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053595996
167 https://doi.org/10.1186/1475-2859-10-104
168 rdf:type schema:CreativeWork
169 sg:pub.10.1186/1475-2859-10-39 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013390138
170 https://doi.org/10.1186/1475-2859-10-39
171 rdf:type schema:CreativeWork
172 sg:pub.10.1186/1746-4269-5-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001230731
173 https://doi.org/10.1186/1746-4269-5-2
174 rdf:type schema:CreativeWork
175 sg:pub.10.1631/jzus.b0820038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014461114
176 https://doi.org/10.1631/jzus.b0820038
177 rdf:type schema:CreativeWork
178 https://app.dimensions.ai/details/publication/pub.1081950181 schema:CreativeWork
179 https://doi.org/10.1002/bab.1378 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009569561
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1002/bit.24547 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038665792
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1002/cmdc.201300059 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034008777
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/j.biortech.2009.03.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047853415
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/j.biortech.2014.01.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037630230
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/j.biortech.2016.06.127 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021196096
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/j.biotechadv.2018.07.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105704604
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/j.bmc.2012.01.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031706812
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/j.copbio.2014.11.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031753111
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/j.jbiotec.2014.04.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020049009
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/j.jchromb.2014.09.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047224904
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/j.molcatb.2010.12.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011919872
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/j.procbio.2014.12.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037850350
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/j.ymben.2014.12.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052909138
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/j.ymben.2016.01.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039476466
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1021/tx800225q schema:sameAs https://app.dimensions.ai/details/publication/pub.1056299890
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1039/c3ay41605d schema:sameAs https://app.dimensions.ai/details/publication/pub.1031702810
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1080/03639045.2017.1402922 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092578204
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1128/mmbr.00025-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022536129
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1155/2016/4305074 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039021937
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1194/jlr.m039834 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010131388
220 rdf:type schema:CreativeWork
221 https://doi.org/10.19540/j.cnki.cjcmm.20180125.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105155413
222 rdf:type schema:CreativeWork
223 https://www.grid.ac/institutes/grid.412540.6 schema:alternateName Shanghai University of Traditional Chinese Medicine
224 schema:name The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People’s Republic of China
225 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...