Enhanced 2,3-butanediol production from biodiesel-derived glycerol by engineering of cofactor regeneration and manipulating carbon flux in Bacillus amyloliquefaciens View Full Text


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Article Info

DATE

2015-12

AUTHORS

Taowei Yang, Zhiming Rao, Xian Zhang, Meijuan Xu, Zhenghong Xu, Shang-Tian Yang

ABSTRACT

BACKGROUND: Bacillus amyloliquefaciens B10-127 exhibited an excellent ability for industrial-scale microbial fermentation of 2,3-butanediol (2,3-BD) from biodiesel-derived glycerol. However, the accumulation of by-products (acetoin, acetoin, lactate and succinate) and the 2,3-BD yield remains prohibitively low for commercial production. RESULTS: Several strategies were developed to manipulate the carbon flux to 2,3-BD branch in a designed B. amyloliquefaciens. Firstly, extra copies of NADH/NAD(+) regeneration system were introduced into B. amyloliquefaciens by co-overproduction of glycerol dehydrogenase and acetoin reductase, which resulting in improvement of 2,3-BD production and suppression of by-products accumulation. Subsequently, the transcriptional regulator ALsR under the control of a moderate promoter PbdhA was introduced into B. amyloliquefaciens, which increased carbon flux to 2,3-BD branch. Finally, a three-stage dissolved oxygen control strategy were proposed based on analysis of the characteristic of 2,3-BD fermentation, and a two-stage pH control strategy were proposed based on different pH preferences of ACR for reduction and oxidation. Following these strategies, a high titer (102.3 g/L), yield (0.44 g/g), and productivity (1.16 g/L/h) of 2,3-BD were achieved. CONCLUSIONS: To our knowledge, this is the highest reported 2,3-BD production using biodiesel-derived glycerol as substrate, and this designed B. amyloliquefaciens should be an excellent candidate for producing 2,3-BD on an industrial scale. More... »

PAGES

122

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12934-015-0317-2

    DOI

    http://dx.doi.org/10.1186/s12934-015-0317-2

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1027355025

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26296537


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    275 https://www.grid.ac/institutes/grid.261331.4 schema:alternateName The Ohio State University
    276 schema:name Department of Chemical and Biomolecular Engineering, The Ohio State University, 43210, Columbus, OH, USA
    277 rdf:type schema:Organization
     




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