Effects and associated transcriptomic landscape changes of methamphetamine on immune cells View Full Text


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Article Info

DATE

2022-06-28

AUTHORS

Deshenyue Kong, Jun-Hong Mao, Hong Li, Jian-Yu Wang, Yu-Yang Li, Xiao-Cong Wu, Guo-Fen Re, Hua-You Luo, Yi-Qun Kuang, Kun-Hua Wang

ABSTRACT

BackgroundMethamphetamine (METH) abuse causes serious health problems, including injury to the immune system, leading to increased incidence of infections and even making withdrawal more difficult. Of course, immune cells, an important part of the immune system, are also injured in methamphetamine abuse. However, due to different research models and the lack of bioinformatics, the mechanism of METH injury to immune cells has not been clarified.MethodsWe examined the response of three common immune cell lines, namely Jurkat, NK-92 and THP-1 cell lines, to methamphetamine by cell viability and apoptosis assay in vitro, and examined their response patterns at the mRNA level by RNA-sequencing. Differential expression analysis of two conditions (control and METH treatment) in three types of immune cells was performed using the DESeq2 R package (1.20.0). And some of the differentially expressed genes were verified by qPCR. We performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of differentially expressed genes by the clusterProfiler R package (3.14.3). And gene enrichment analysis was also performed using MetaScape (www.metascape.org).ResultsThe viability of the three immune cells was differentially affected by methamphetamine, and the rate of NK-cell apoptosis was significantly increased. At the mRNA level, we found disorders of cholesterol metabolism in Jurkat cells, activation of ERK1 and ERK2 cascade in NK-92 cells, and disruption of calcium transport channels in THP-1 cells. In addition, all three cells showed changes in the phospholipid metabolic process.ConclusionsThe results suggest that both innate and adaptive immune cells are affected by METH abuse, and there may be commonalities between different immune cells at the transcriptome level. These results provide new insights into the potential effects by which METH injures the immune cells. More... »

PAGES

144

References to SciGraph publications

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    29 schema:description BackgroundMethamphetamine (METH) abuse causes serious health problems, including injury to the immune system, leading to increased incidence of infections and even making withdrawal more difficult. Of course, immune cells, an important part of the immune system, are also injured in methamphetamine abuse. However, due to different research models and the lack of bioinformatics, the mechanism of METH injury to immune cells has not been clarified.MethodsWe examined the response of three common immune cell lines, namely Jurkat, NK-92 and THP-1 cell lines, to methamphetamine by cell viability and apoptosis assay in vitro, and examined their response patterns at the mRNA level by RNA-sequencing. Differential expression analysis of two conditions (control and METH treatment) in three types of immune cells was performed using the DESeq2 R package (1.20.0). And some of the differentially expressed genes were verified by qPCR. We performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of differentially expressed genes by the clusterProfiler R package (3.14.3). And gene enrichment analysis was also performed using MetaScape (www.metascape.org).ResultsThe viability of the three immune cells was differentially affected by methamphetamine, and the rate of NK-cell apoptosis was significantly increased. At the mRNA level, we found disorders of cholesterol metabolism in Jurkat cells, activation of ERK1 and ERK2 cascade in NK-92 cells, and disruption of calcium transport channels in THP-1 cells. In addition, all three cells showed changes in the phospholipid metabolic process.ConclusionsThe results suggest that both innate and adaptive immune cells are affected by METH abuse, and there may be commonalities between different immune cells at the transcriptome level. These results provide new insights into the potential effects by which METH injures the immune cells.
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    36 DESeq2 R package
    37 ERK1
    38 ERK2
    39 Encyclopedia
    40 Gene Ontology
    41 Jurkat
    42 Jurkat cells
    43 Kyoto Encyclopedia
    44 METH
    45 METH abuse
    46 Metascape
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    48 NK cell apoptosis
    49 NK-92
    50 NK-92 cells
    51 R package
    52 RNA sequencing
    53 THP-1 cell line
    54 THP-1 cells
    55 abuse
    56 activation
    57 activation of ERK1
    58 adaptive immune cells
    59 addition
    60 analysis
    61 apoptosis
    62 bioinformatics
    63 cell lines
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    65 cells
    66 changes
    67 channels
    68 cholesterol metabolism
    69 clusterProfiler R package
    70 commonalities
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    72 different immune cells
    73 different research models
    74 differential expression analysis
    75 disorders
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    77 effect
    78 enrichment analysis
    79 expression analysis
    80 gene enrichment analysis
    81 genes
    82 genome analysis
    83 health problems
    84 immune cell lines
    85 immune cells
    86 immune system
    87 important part
    88 incidence
    89 incidence of infection
    90 infection
    91 injury
    92 insights
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    94 landscape change
    95 levels
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    97 mRNA levels
    98 mechanism
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    101 methamphetamine
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