Occurrence of extended-spectrum beta-lactamase-producing Enterobacteriaceae, microbial loads, and endotoxin levels in dust from laying hen houses in Egypt View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2020-08-24

AUTHORS

Marwa F. E. Ahmed, Hazem Ramadan, Diana Seinige, Corinna Kehrenberg, Amr Abd El-Wahab, Nina Volkmann, Nicole Kemper, Jochen Schulz

ABSTRACT

BackgroundPoultry houses are often highly contaminated with dust, which might contain considerable amounts of microorganisms and endotoxins. The concentrations of microorganisms and endotoxins in dust from laying hen houses in Egypt are unknown. However, to estimate the risks for birds, the environment, and people working in laying hen houses, it is important to gather information about the composition of these dusts. Here we report the microbial loads, the occurrence of antimicrobial-resistant bacteria, and endotoxin concentrations in dust samples from 28 laying hen farms in Dakahliya Governorate, Egypt, and discuss the results relevant to the literature.ResultsPooled settled dust samples (n = 28) were analyzed for total viable counts of bacteria and fungi (CFU/g), the occurrence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae, Salmonella spp., and methicillin-resistant Staphylococcus aureus (MRSA), and endotoxin concentrations (ng/g). The means and standard deviations of total viable counts were 7.10 × 108 ± 2.55 × 109 CFU/g for bacteria and 5.37 × 106 ± 7.26 × 106 CFU/g for fungi. Endotoxin levels varied from 2.9 × 104 to 6.27 × 105 ng/g. None of the tested samples contained Salmonella spp. or MRSA. In contrast, by direct plating, Enterobacteriaceae were found frequently (57%; n = 16), and suspected ESBL-producing Enterobacteriaceae occurred in 21% (n = 6) of the sampled barns. Using an enrichment method, the detection of Enterobacteriaceae and suspected ESBL-producing Enterobacteriaceae increased to 20 and 16 positive barns, respectively. Taking results from both methods into account, Enterobacteriaceae and suspected ESBL-producing Enterobacteriaceae were detected in 23 barns Overall, 100 ESBL suspected isolates (Escherichia coli, n = 64; Enterobacter cloacae, n = 20; and Klebsiella pneumoniae n = 16) were identified to species level by MALDI-TOF MS. Isolates from 20 barns (71% positive barns) were confirmed as ESBL producing Enterobacteriaceae by the broth microdilution test.ConclusionsDust in Egyptian laying hen houses contains high concentrations of microorganisms and endotoxins, which might impair the health of birds and farmers when inhaled. Furthermore, laying hens in Egypt seem to be a reservoir for ESBL-producing Enterobacteriaceae. Thus, farmers are at risk of exposure to ESBL-producing bacteria, and colonized hens might transmit these bacteria into the food chain. More... »

PAGES

301

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12917-020-02510-4

DOI

http://dx.doi.org/10.1186/s12917-020-02510-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1130308310

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/32838780


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chickens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Resistance, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dust", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Egypt", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endotoxins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enterobacteriaceae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fungi", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Housing, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Occupational Exposure", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "beta-Lactamases", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt", 
          "id": "http://www.grid.ac/institutes/grid.10251.37", 
          "name": [
            "Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany", 
            "Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ahmed", 
        "givenName": "Marwa F. E.", 
        "id": "sg:person.01256560554.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256560554.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt", 
          "id": "http://www.grid.ac/institutes/grid.10251.37", 
          "name": [
            "Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ramadan", 
        "givenName": "Hazem", 
        "id": "sg:person.015647337701.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015647337701.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany", 
          "id": "http://www.grid.ac/institutes/grid.412970.9", 
          "name": [
            "Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Seinige", 
        "givenName": "Diana", 
        "id": "sg:person.01147410276.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147410276.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute for Veterinary Food Science, Justus-Liebig-University Giessen, Giessen, Germany", 
          "id": "http://www.grid.ac/institutes/grid.8664.c", 
          "name": [
            "Institute for Veterinary Food Science, Justus-Liebig-University Giessen, Giessen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kehrenberg", 
        "givenName": "Corinna", 
        "id": "sg:person.01274256460.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274256460.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nutrition and Nutritional Deficiency Diseases Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt", 
          "id": "http://www.grid.ac/institutes/grid.10251.37", 
          "name": [
            "Nutrition and Nutritional Deficiency Diseases Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Abd El-Wahab", 
        "givenName": "Amr", 
        "id": "sg:person.0623453504.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623453504.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany", 
          "id": "http://www.grid.ac/institutes/grid.412970.9", 
          "name": [
            "Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Volkmann", 
        "givenName": "Nina", 
        "id": "sg:person.012370607274.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012370607274.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany", 
          "id": "http://www.grid.ac/institutes/grid.412970.9", 
          "name": [
            "Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kemper", 
        "givenName": "Nicole", 
        "id": "sg:person.0772053320.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772053320.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany", 
          "id": "http://www.grid.ac/institutes/grid.412970.9", 
          "name": [
            "Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schulz", 
        "givenName": "Jochen", 
        "id": "sg:person.01054434355.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054434355.17"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1016/j.bjm.2015.11.034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023775585", 
          "https://doi.org/10.1016/j.bjm.2015.11.034"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s000110050751", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039940893", 
          "https://doi.org/10.1007/s000110050751"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10453-010-9158-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006670909", 
          "https://doi.org/10.1007/s10453-010-9158-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-019-41528-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1112983726", 
          "https://doi.org/10.1038/s41598-019-41528-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10661-014-4218-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031457142", 
          "https://doi.org/10.1007/s10661-014-4218-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1745-6673-4-13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008648744", 
          "https://doi.org/10.1186/1745-6673-4-13"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/jes.2014.85", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039009223", 
          "https://doi.org/10.1038/jes.2014.85"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.2478/s13382-012-0032-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037030575", 
          "https://doi.org/10.2478/s13382-012-0032-8"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2020-08-24", 
    "datePublishedReg": "2020-08-24", 
    "description": "BackgroundPoultry houses are often highly contaminated with dust, which might contain considerable amounts of microorganisms and endotoxins. The concentrations of microorganisms and endotoxins in dust from laying hen houses in Egypt are unknown. However, to estimate the risks for birds, the environment, and people working in laying hen houses, it is important to gather information about the composition of these dusts. Here we report the microbial loads, the occurrence of antimicrobial-resistant bacteria, and endotoxin concentrations in dust samples from 28 laying hen farms in Dakahliya Governorate, Egypt, and discuss the results relevant to the literature.ResultsPooled settled dust samples (n\u2009=\u200928) were analyzed for total viable counts of bacteria and fungi (CFU/g), the occurrence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae, Salmonella spp., and methicillin-resistant Staphylococcus aureus (MRSA), and endotoxin concentrations (ng/g). The means and standard deviations of total viable counts were 7.10\u2009\u00d7\u2009108\u2009\u00b1\u20092.55\u2009\u00d7\u2009109\u2009CFU/g for bacteria and 5.37\u2009\u00d7\u2009106\u2009\u00b1\u20097.26\u2009\u00d7\u2009106\u2009CFU/g for fungi. Endotoxin levels varied from 2.9\u2009\u00d7\u2009104 to 6.27\u2009\u00d7\u2009105\u2009ng/g. None of the tested samples contained Salmonella spp. or MRSA. In contrast, by direct plating, Enterobacteriaceae were found frequently (57%; n\u2009=\u200916), and suspected ESBL-producing Enterobacteriaceae occurred in 21% (n\u2009=\u20096) of the sampled barns. Using an enrichment method, the detection of Enterobacteriaceae and suspected ESBL-producing Enterobacteriaceae increased to 20 and 16 positive barns, respectively. Taking results from both methods into account, Enterobacteriaceae and suspected ESBL-producing Enterobacteriaceae were detected in 23 barns Overall, 100 ESBL suspected isolates (Escherichia coli, n\u2009=\u200964; Enterobacter cloacae, n\u2009=\u200920; and Klebsiella pneumoniae n\u2009=\u200916) were identified to species level by MALDI-TOF MS. Isolates from 20 barns (71% positive barns) were confirmed as ESBL producing Enterobacteriaceae by the broth microdilution test.ConclusionsDust in Egyptian laying hen houses contains high concentrations of microorganisms and endotoxins, which might impair the health of birds and farmers when inhaled. Furthermore, laying hens in Egypt seem to be a reservoir for ESBL-producing Enterobacteriaceae. Thus, farmers are at risk of exposure to ESBL-producing bacteria, and colonized hens might transmit these bacteria into the food chain.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/s12917-020-02510-4", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1035490", 
        "issn": [
          "1746-6148"
        ], 
        "name": "BMC Veterinary Research", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "16"
      }
    ], 
    "keywords": [
      "methicillin-resistant Staphylococcus aureus", 
      "ESBL-producing Enterobacteriaceae", 
      "endotoxin levels", 
      "broth microdilution test", 
      "risk of exposure", 
      "Salmonella spp", 
      "antimicrobial-resistant bacteria", 
      "endotoxin concentrations", 
      "ESBL", 
      "microdilution test", 
      "endotoxin", 
      "Enterobacteriaceae", 
      "Staphylococcus aureus", 
      "detection of Enterobacteriaceae", 
      "dust samples", 
      "CFU/", 
      "risk", 
      "viable counts", 
      "count", 
      "health of birds", 
      "hens", 
      "microbial load", 
      "isolates", 
      "total viable count", 
      "levels", 
      "hen farms", 
      "direct plating", 
      "Overall", 
      "high concentrations", 
      "occurrence", 
      "concentration", 
      "bacteria", 
      "aureus", 
      "health", 
      "exposure", 
      "Dakahliya Governorate", 
      "MALDI-TOF MS", 
      "samples", 
      "spp", 
      "hen houses", 
      "species level", 
      "microorganisms", 
      "contrast", 
      "test", 
      "Egypt", 
      "people", 
      "standard deviation", 
      "birds", 
      "results", 
      "Governorate", 
      "MS", 
      "literature", 
      "plating", 
      "barn", 
      "enrichment method", 
      "detection", 
      "food chain", 
      "considerable amount", 
      "dust", 
      "concentration of microorganisms", 
      "method", 
      "fungi", 
      "information", 
      "load", 
      "means", 
      "house", 
      "amount", 
      "deviation", 
      "farms", 
      "reservoir", 
      "composition", 
      "chain", 
      "environment", 
      "account", 
      "farmers"
    ], 
    "name": "Occurrence of extended-spectrum beta-lactamase-producing Enterobacteriaceae, microbial loads, and endotoxin levels in dust from laying hen houses in Egypt", 
    "pagination": "301", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1130308310"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12917-020-02510-4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "32838780"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12917-020-02510-4", 
      "https://app.dimensions.ai/details/publication/pub.1130308310"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-20T07:36", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_833.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/s12917-020-02510-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12917-020-02510-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12917-020-02510-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12917-020-02510-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12917-020-02510-4'


 

This table displays all metadata directly associated to this object as RDF triples.

276 TRIPLES      22 PREDICATES      121 URIs      105 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12917-020-02510-4 schema:about N4f836d7041cd40c88e8e18461a0c6a87
2 N5975ee7aa1dd4bf2be41643b1dc3782e
3 N6306cf77c0bd450f981e414e703bcab3
4 N6a142a31edea4031a47e8bb52fa2e608
5 N71a120f3f60d41d3916bd89018763df6
6 N7e0e181959d94c1b9fb0e74e091ee891
7 N7f3e8b4660fa4ba29a07830fa2b5cac2
8 N7f48520845eb4158bae154cd2eb251ff
9 N8159feaa03444a36b14fd4724128c72f
10 N97ffcaed5d9448139bbda428b8a2ea5f
11 Na75c92b1a7084a2db9021aa3990ffe4b
12 Nb4bd1c44744846919070636c5671b403
13 anzsrc-for:06
14 anzsrc-for:0605
15 schema:author Nfaf9ae23ef244f3ca1584dd50f628113
16 schema:citation sg:pub.10.1007/s000110050751
17 sg:pub.10.1007/s10453-010-9158-y
18 sg:pub.10.1007/s10661-014-4218-3
19 sg:pub.10.1016/j.bjm.2015.11.034
20 sg:pub.10.1038/jes.2014.85
21 sg:pub.10.1038/s41598-019-41528-z
22 sg:pub.10.1186/1745-6673-4-13
23 sg:pub.10.2478/s13382-012-0032-8
24 schema:datePublished 2020-08-24
25 schema:datePublishedReg 2020-08-24
26 schema:description BackgroundPoultry houses are often highly contaminated with dust, which might contain considerable amounts of microorganisms and endotoxins. The concentrations of microorganisms and endotoxins in dust from laying hen houses in Egypt are unknown. However, to estimate the risks for birds, the environment, and people working in laying hen houses, it is important to gather information about the composition of these dusts. Here we report the microbial loads, the occurrence of antimicrobial-resistant bacteria, and endotoxin concentrations in dust samples from 28 laying hen farms in Dakahliya Governorate, Egypt, and discuss the results relevant to the literature.ResultsPooled settled dust samples (n = 28) were analyzed for total viable counts of bacteria and fungi (CFU/g), the occurrence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae, Salmonella spp., and methicillin-resistant Staphylococcus aureus (MRSA), and endotoxin concentrations (ng/g). The means and standard deviations of total viable counts were 7.10 × 108 ± 2.55 × 109 CFU/g for bacteria and 5.37 × 106 ± 7.26 × 106 CFU/g for fungi. Endotoxin levels varied from 2.9 × 104 to 6.27 × 105 ng/g. None of the tested samples contained Salmonella spp. or MRSA. In contrast, by direct plating, Enterobacteriaceae were found frequently (57%; n = 16), and suspected ESBL-producing Enterobacteriaceae occurred in 21% (n = 6) of the sampled barns. Using an enrichment method, the detection of Enterobacteriaceae and suspected ESBL-producing Enterobacteriaceae increased to 20 and 16 positive barns, respectively. Taking results from both methods into account, Enterobacteriaceae and suspected ESBL-producing Enterobacteriaceae were detected in 23 barns Overall, 100 ESBL suspected isolates (Escherichia coli, n = 64; Enterobacter cloacae, n = 20; and Klebsiella pneumoniae n = 16) were identified to species level by MALDI-TOF MS. Isolates from 20 barns (71% positive barns) were confirmed as ESBL producing Enterobacteriaceae by the broth microdilution test.ConclusionsDust in Egyptian laying hen houses contains high concentrations of microorganisms and endotoxins, which might impair the health of birds and farmers when inhaled. Furthermore, laying hens in Egypt seem to be a reservoir for ESBL-producing Enterobacteriaceae. Thus, farmers are at risk of exposure to ESBL-producing bacteria, and colonized hens might transmit these bacteria into the food chain.
27 schema:genre article
28 schema:inLanguage en
29 schema:isAccessibleForFree true
30 schema:isPartOf N430f7026e93345669959092f462b9619
31 N663fb406aa7342d8a36045bc3abcf90d
32 sg:journal.1035490
33 schema:keywords CFU/
34 Dakahliya Governorate
35 ESBL
36 ESBL-producing Enterobacteriaceae
37 Egypt
38 Enterobacteriaceae
39 Governorate
40 MALDI-TOF MS
41 MS
42 Overall
43 Salmonella spp
44 Staphylococcus aureus
45 account
46 amount
47 antimicrobial-resistant bacteria
48 aureus
49 bacteria
50 barn
51 birds
52 broth microdilution test
53 chain
54 composition
55 concentration
56 concentration of microorganisms
57 considerable amount
58 contrast
59 count
60 detection
61 detection of Enterobacteriaceae
62 deviation
63 direct plating
64 dust
65 dust samples
66 endotoxin
67 endotoxin concentrations
68 endotoxin levels
69 enrichment method
70 environment
71 exposure
72 farmers
73 farms
74 food chain
75 fungi
76 health
77 health of birds
78 hen farms
79 hen houses
80 hens
81 high concentrations
82 house
83 information
84 isolates
85 levels
86 literature
87 load
88 means
89 methicillin-resistant Staphylococcus aureus
90 method
91 microbial load
92 microdilution test
93 microorganisms
94 occurrence
95 people
96 plating
97 reservoir
98 results
99 risk
100 risk of exposure
101 samples
102 species level
103 spp
104 standard deviation
105 test
106 total viable count
107 viable counts
108 schema:name Occurrence of extended-spectrum beta-lactamase-producing Enterobacteriaceae, microbial loads, and endotoxin levels in dust from laying hen houses in Egypt
109 schema:pagination 301
110 schema:productId N0d3be372adf34f54b8cc12d7cb23ef6f
111 N2b1c453c6f9e4540a6cfcae9369f9637
112 Nce7fccdab377430b89005a03cde0d1d5
113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1130308310
114 https://doi.org/10.1186/s12917-020-02510-4
115 schema:sdDatePublished 2022-05-20T07:36
116 schema:sdLicense https://scigraph.springernature.com/explorer/license/
117 schema:sdPublisher Nb00f84d40d76451cb7cb95a40a9a5201
118 schema:url https://doi.org/10.1186/s12917-020-02510-4
119 sgo:license sg:explorer/license/
120 sgo:sdDataset articles
121 rdf:type schema:ScholarlyArticle
122 N0a7c16328f6d4a70bac571ab7487d09a rdf:first sg:person.01054434355.17
123 rdf:rest rdf:nil
124 N0d3be372adf34f54b8cc12d7cb23ef6f schema:name dimensions_id
125 schema:value pub.1130308310
126 rdf:type schema:PropertyValue
127 N0f2e533000c14d388985203917a070f6 rdf:first sg:person.0623453504.39
128 rdf:rest N320acbf0abac4fd9b32f0bc5afc46f75
129 N2b1c453c6f9e4540a6cfcae9369f9637 schema:name pubmed_id
130 schema:value 32838780
131 rdf:type schema:PropertyValue
132 N320acbf0abac4fd9b32f0bc5afc46f75 rdf:first sg:person.012370607274.60
133 rdf:rest Nbe2f62a3243b471aa49363488cb11765
134 N430f7026e93345669959092f462b9619 schema:volumeNumber 16
135 rdf:type schema:PublicationVolume
136 N4f836d7041cd40c88e8e18461a0c6a87 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Fungi
138 rdf:type schema:DefinedTerm
139 N5975ee7aa1dd4bf2be41643b1dc3782e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name beta-Lactamases
141 rdf:type schema:DefinedTerm
142 N6306cf77c0bd450f981e414e703bcab3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Enterobacteriaceae
144 rdf:type schema:DefinedTerm
145 N663fb406aa7342d8a36045bc3abcf90d schema:issueNumber 1
146 rdf:type schema:PublicationIssue
147 N6a142a31edea4031a47e8bb52fa2e608 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Housing, Animal
149 rdf:type schema:DefinedTerm
150 N71a120f3f60d41d3916bd89018763df6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Female
152 rdf:type schema:DefinedTerm
153 N7e0e181959d94c1b9fb0e74e091ee891 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Occupational Exposure
155 rdf:type schema:DefinedTerm
156 N7f3e8b4660fa4ba29a07830fa2b5cac2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Drug Resistance, Bacterial
158 rdf:type schema:DefinedTerm
159 N7f48520845eb4158bae154cd2eb251ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Endotoxins
161 rdf:type schema:DefinedTerm
162 N8159feaa03444a36b14fd4724128c72f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Dust
164 rdf:type schema:DefinedTerm
165 N918b222fcbf04fa984d1a01debc47275 rdf:first sg:person.015647337701.01
166 rdf:rest N9fd5dccfd510468380f33d6969c139fb
167 N97ffcaed5d9448139bbda428b8a2ea5f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Egypt
169 rdf:type schema:DefinedTerm
170 N9fd5dccfd510468380f33d6969c139fb rdf:first sg:person.01147410276.53
171 rdf:rest Nbad8a8e4ef70403eb61f48330f2af6f5
172 Na75c92b1a7084a2db9021aa3990ffe4b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Chickens
174 rdf:type schema:DefinedTerm
175 Nb00f84d40d76451cb7cb95a40a9a5201 schema:name Springer Nature - SN SciGraph project
176 rdf:type schema:Organization
177 Nb4bd1c44744846919070636c5671b403 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Animals
179 rdf:type schema:DefinedTerm
180 Nbad8a8e4ef70403eb61f48330f2af6f5 rdf:first sg:person.01274256460.10
181 rdf:rest N0f2e533000c14d388985203917a070f6
182 Nbe2f62a3243b471aa49363488cb11765 rdf:first sg:person.0772053320.07
183 rdf:rest N0a7c16328f6d4a70bac571ab7487d09a
184 Nce7fccdab377430b89005a03cde0d1d5 schema:name doi
185 schema:value 10.1186/s12917-020-02510-4
186 rdf:type schema:PropertyValue
187 Nfaf9ae23ef244f3ca1584dd50f628113 rdf:first sg:person.01256560554.23
188 rdf:rest N918b222fcbf04fa984d1a01debc47275
189 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
190 schema:name Biological Sciences
191 rdf:type schema:DefinedTerm
192 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
193 schema:name Microbiology
194 rdf:type schema:DefinedTerm
195 sg:journal.1035490 schema:issn 1746-6148
196 schema:name BMC Veterinary Research
197 schema:publisher Springer Nature
198 rdf:type schema:Periodical
199 sg:person.01054434355.17 schema:affiliation grid-institutes:grid.412970.9
200 schema:familyName Schulz
201 schema:givenName Jochen
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054434355.17
203 rdf:type schema:Person
204 sg:person.01147410276.53 schema:affiliation grid-institutes:grid.412970.9
205 schema:familyName Seinige
206 schema:givenName Diana
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147410276.53
208 rdf:type schema:Person
209 sg:person.012370607274.60 schema:affiliation grid-institutes:grid.412970.9
210 schema:familyName Volkmann
211 schema:givenName Nina
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012370607274.60
213 rdf:type schema:Person
214 sg:person.01256560554.23 schema:affiliation grid-institutes:grid.10251.37
215 schema:familyName Ahmed
216 schema:givenName Marwa F. E.
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256560554.23
218 rdf:type schema:Person
219 sg:person.01274256460.10 schema:affiliation grid-institutes:grid.8664.c
220 schema:familyName Kehrenberg
221 schema:givenName Corinna
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274256460.10
223 rdf:type schema:Person
224 sg:person.015647337701.01 schema:affiliation grid-institutes:grid.10251.37
225 schema:familyName Ramadan
226 schema:givenName Hazem
227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015647337701.01
228 rdf:type schema:Person
229 sg:person.0623453504.39 schema:affiliation grid-institutes:grid.10251.37
230 schema:familyName Abd El-Wahab
231 schema:givenName Amr
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623453504.39
233 rdf:type schema:Person
234 sg:person.0772053320.07 schema:affiliation grid-institutes:grid.412970.9
235 schema:familyName Kemper
236 schema:givenName Nicole
237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772053320.07
238 rdf:type schema:Person
239 sg:pub.10.1007/s000110050751 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039940893
240 https://doi.org/10.1007/s000110050751
241 rdf:type schema:CreativeWork
242 sg:pub.10.1007/s10453-010-9158-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1006670909
243 https://doi.org/10.1007/s10453-010-9158-y
244 rdf:type schema:CreativeWork
245 sg:pub.10.1007/s10661-014-4218-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031457142
246 https://doi.org/10.1007/s10661-014-4218-3
247 rdf:type schema:CreativeWork
248 sg:pub.10.1016/j.bjm.2015.11.034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023775585
249 https://doi.org/10.1016/j.bjm.2015.11.034
250 rdf:type schema:CreativeWork
251 sg:pub.10.1038/jes.2014.85 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039009223
252 https://doi.org/10.1038/jes.2014.85
253 rdf:type schema:CreativeWork
254 sg:pub.10.1038/s41598-019-41528-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1112983726
255 https://doi.org/10.1038/s41598-019-41528-z
256 rdf:type schema:CreativeWork
257 sg:pub.10.1186/1745-6673-4-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008648744
258 https://doi.org/10.1186/1745-6673-4-13
259 rdf:type schema:CreativeWork
260 sg:pub.10.2478/s13382-012-0032-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037030575
261 https://doi.org/10.2478/s13382-012-0032-8
262 rdf:type schema:CreativeWork
263 grid-institutes:grid.10251.37 schema:alternateName Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
264 Nutrition and Nutritional Deficiency Diseases Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
265 schema:name Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
266 Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
267 Nutrition and Nutritional Deficiency Diseases Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
268 rdf:type schema:Organization
269 grid-institutes:grid.412970.9 schema:alternateName Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
270 Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
271 schema:name Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
272 Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany
273 rdf:type schema:Organization
274 grid-institutes:grid.8664.c schema:alternateName Institute for Veterinary Food Science, Justus-Liebig-University Giessen, Giessen, Germany
275 schema:name Institute for Veterinary Food Science, Justus-Liebig-University Giessen, Giessen, Germany
276 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...