Characterization of minimal lesions related to the presence of visna/maedi virus in the mammary gland and milk of dairy sheep. View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

E Gayo, L Polledo, A Magalde, A Balseiro, M J García Iglesias, C Pérez Martínez, S Preziuso, G Rossi, J F García Marín

ABSTRACT

BACKGROUND: In order to characterize the complete range of lesions, especially minimal, affecting mammary gland and viral antigen distribution and target cells using immunohistochemistry in naturally Visna/maedi (VM) 84 infected sheep were studied, forty-four from flocks with clinical cases (A) and 35 randomly sampled from two abattoirs (B) together with five negative controls (C). An immunocytochemistry technique was developed and further milk samples (n = 39) were used to study viral excretion, carrier cells and the role of milk and colostrum in the transmission of the disease. RESULTS: All sheep from group C and three sheep from group B were negative to VM in tissue sections by histopathology, immunohistochemistry and PCR, and also in serum using ELISA. Several degrees of CD3 + lymphocytic interstitial mastitis were observed in groups A and B: minimal (+) n = 26 sheep; moderate (++), n = 32 and severe (+++), n = 12. No differences in lesion distribution were observed between groups A and B. Viral presence was confirmed by immunohistochemistry using two different antibodies and/or PCR in every tissue with lesions while serology was negative in six sheep with lesions. Two milk samples taken from milk tanks from two flocks from group A and fourteen milk samples from 29 infected sheep from group B were positive to VM (most of them from animals with moderate and severe lesions). Positivity was only found in macrophages, even in focal and minimal lesions, while no positivity was observed in epithelial or any other cells in either tissue and milk samples. CONCLUSIONS: This new observation of the minimal lesions described in this work increased the prevalence of VM lesions in mammary gland up to 90.9% and VM should be considered as a differential diagnosis when minimal interstitial lesions are detected. A high prevalence of VM was observed in intensive milk-producing sheep, ELISA serology did not detect as positivity all infected animals, while histology, IHC or PCR showed higher sensitivity. The cytological technique developed was very useful in milk-cell studies using hematoxylin and eosin and immunocytochemistry. Viral detection in milk samples (16/39) confirms a potential but limited role of milk/colostrum in viral transmission. More... »

PAGES

109

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12917-019-1855-3

DOI

http://dx.doi.org/10.1186/s12917-019-1855-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1113327583

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30967151


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Leon", 
          "id": "https://www.grid.ac/institutes/grid.4807.b", 
          "name": [
            "Veterinary Pathology Unit, School of Veterinary Medicine, University of Le\u00f3n, Le\u00f3n, Spain. egayr@unileon.es."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gayo", 
        "givenName": "E", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Micros Veterinaria, Le\u00f3n, Spain."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Polledo", 
        "givenName": "L", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Leon", 
          "id": "https://www.grid.ac/institutes/grid.4807.b", 
          "name": [
            "Veterinary Pathology Unit, School of Veterinary Medicine, University of Le\u00f3n, Le\u00f3n, Spain."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Magalde", 
        "givenName": "A", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Servicio Regional de Investigaci\u00f3n y Desarrollo Agroalimentario", 
          "id": "https://www.grid.ac/institutes/grid.419063.9", 
          "name": [
            "SERIDA, Gij\u00f3n, Spain."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Balseiro", 
        "givenName": "A", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Leon", 
          "id": "https://www.grid.ac/institutes/grid.4807.b", 
          "name": [
            "Veterinary Pathology Unit, School of Veterinary Medicine, University of Le\u00f3n, Le\u00f3n, Spain."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Garc\u00eda Iglesias", 
        "givenName": "M J", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Leon", 
          "id": "https://www.grid.ac/institutes/grid.4807.b", 
          "name": [
            "Veterinary Pathology Unit, School of Veterinary Medicine, University of Le\u00f3n, Le\u00f3n, Spain."
          ], 
          "type": "Organization"
        }, 
        "familyName": "P\u00e9rez Mart\u00ednez", 
        "givenName": "C", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Camerino", 
          "id": "https://www.grid.ac/institutes/grid.5602.1", 
          "name": [
            "School of Bioscences and Veterinary Medicine, University of Camerino, Camerino, Italy."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Preziuso", 
        "givenName": "S", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Camerino", 
          "id": "https://www.grid.ac/institutes/grid.5602.1", 
          "name": [
            "School of Bioscences and Veterinary Medicine, University of Camerino, Camerino, Italy."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rossi", 
        "givenName": "G", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Leon", 
          "id": "https://www.grid.ac/institutes/grid.4807.b", 
          "name": [
            "Veterinary Pathology Unit, School of Veterinary Medicine, University of Le\u00f3n, Le\u00f3n, Spain."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Garc\u00eda Mar\u00edn", 
        "givenName": "J F", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.cimid.2011.12.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000161215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tvjl.2009.07.031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001683902"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-5877(03)00163-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002380539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-5877(03)00163-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002380539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jviromet.2006.07.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003160498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.rvsc.2005.05.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005854619"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0166-0934(02)00208-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008747170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0166-0934(02)00208-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008747170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetmic.2005.01.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010971498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetmic.2004.09.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014304851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.meegid.2010.06.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020638942"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1751-0813.1986.tb02984.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022679922"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/030098589403100610", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022680241"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/030098589403100610", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022680241"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.82.20.7086", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024802212"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jcpa.2011.09.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025781656"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s13365-012-0131-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028540790", 
          "https://doi.org/10.1007/s13365-012-0131-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetmic.2015.08.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028825469"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tvjl.2013.03.031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032859673"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/104063879901100507", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035129371"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/104063879901100507", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035129371"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/030098587100800108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036211001"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/030098587100800108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036211001"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.74.21.10096-10103.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036795848"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.smallrumres.2012.12.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043531085"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1439-0264.2006.00720.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043724988"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(85)90246-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044421010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.rvsc.2004.09.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045110516"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00005072-195707000-00010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045373292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1751-0813.1983.tb02832.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050211924"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0022029900021646", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053965614"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0022029900021646", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053965614"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1051/vetres:2005048", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057072223"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/vr.129.3.51", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062831063"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074543048", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4081/849", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1076620712"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077178040", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0034-5288(18)31108-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078999620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081650294", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081656785", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082536152", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082788420", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083300362", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.smallrumres.2017.10.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092317738"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jcpa.2018.01.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101320428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jcpa.2018.01.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101320428"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "BACKGROUND: In order to characterize the complete range of lesions, especially minimal, affecting mammary gland and viral antigen distribution and target cells using immunohistochemistry in naturally Visna/maedi (VM) 84 infected sheep were studied, forty-four from flocks with clinical cases (A) and 35 randomly sampled from two abattoirs (B) together with five negative controls (C). An immunocytochemistry technique was developed and further milk samples (n\u2009=\u200939) were used to study viral excretion, carrier cells and the role of milk and colostrum in the transmission of the disease.\nRESULTS: All sheep from group C and three sheep from group B were negative to VM in tissue sections by histopathology, immunohistochemistry and PCR, and also in serum using ELISA. Several degrees of CD3\u2009+\u2009lymphocytic interstitial mastitis were observed in groups A and B: minimal (+) n\u2009=\u200926 sheep; moderate (++), n\u2009=\u200932 and severe (+++), n\u2009=\u200912. No differences in lesion distribution were observed between groups A and B. Viral presence was confirmed by immunohistochemistry using two different antibodies and/or PCR in every tissue with lesions while serology was negative in six sheep with lesions. Two milk samples taken from milk tanks from two flocks from group A and fourteen milk samples from 29 infected sheep from group B were positive to VM (most of them from animals with moderate and severe lesions). Positivity was only found in macrophages, even in focal and minimal lesions, while no positivity was observed in epithelial or any other cells in either tissue and milk samples.\nCONCLUSIONS: This new observation of the minimal lesions described in this work increased the prevalence of VM lesions in mammary gland up to 90.9% and VM should be considered as a differential diagnosis when minimal interstitial lesions are detected. A high prevalence of VM was observed in intensive milk-producing sheep, ELISA serology did not detect as positivity all infected animals, while histology, IHC or PCR showed higher sensitivity. The cytological technique developed was very useful in milk-cell studies using hematoxylin and eosin and immunocytochemistry. Viral detection in milk samples (16/39) confirms a potential but limited role of milk/colostrum in viral transmission.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s12917-019-1855-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1035490", 
        "issn": [
          "1746-6148"
        ], 
        "name": "BMC Veterinary Research", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "name": "Characterization of minimal lesions related to the presence of visna/maedi virus in the mammary gland and milk of dairy sheep.", 
    "pagination": "109", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12917-019-1855-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1113327583"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101249759"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30967151"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12917-019-1855-3", 
      "https://app.dimensions.ai/details/publication/pub.1113327583"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-15T09:13", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000376_0000000376/records_56159_00000006.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-019-1855-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12917-019-1855-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12917-019-1855-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12917-019-1855-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12917-019-1855-3'


 

This table displays all metadata directly associated to this object as RDF triples.

231 TRIPLES      21 PREDICATES      67 URIs      20 LITERALS      8 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12917-019-1855-3 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N1c3efef54e7443c5aff3a60a82d3d53c
4 schema:citation sg:pub.10.1007/s13365-012-0131-0
5 https://app.dimensions.ai/details/publication/pub.1074543048
6 https://app.dimensions.ai/details/publication/pub.1077178040
7 https://app.dimensions.ai/details/publication/pub.1081650294
8 https://app.dimensions.ai/details/publication/pub.1081656785
9 https://app.dimensions.ai/details/publication/pub.1082536152
10 https://app.dimensions.ai/details/publication/pub.1082788420
11 https://app.dimensions.ai/details/publication/pub.1083300362
12 https://doi.org/10.1016/0042-6822(85)90246-6
13 https://doi.org/10.1016/j.cimid.2011.12.003
14 https://doi.org/10.1016/j.jcpa.2011.09.003
15 https://doi.org/10.1016/j.jcpa.2018.01.001
16 https://doi.org/10.1016/j.jviromet.2006.07.009
17 https://doi.org/10.1016/j.meegid.2010.06.007
18 https://doi.org/10.1016/j.rvsc.2004.09.006
19 https://doi.org/10.1016/j.rvsc.2005.05.003
20 https://doi.org/10.1016/j.smallrumres.2012.12.010
21 https://doi.org/10.1016/j.smallrumres.2017.10.008
22 https://doi.org/10.1016/j.tvjl.2009.07.031
23 https://doi.org/10.1016/j.tvjl.2013.03.031
24 https://doi.org/10.1016/j.vetmic.2004.09.010
25 https://doi.org/10.1016/j.vetmic.2005.01.012
26 https://doi.org/10.1016/j.vetmic.2015.08.007
27 https://doi.org/10.1016/s0034-5288(18)31108-1
28 https://doi.org/10.1016/s0166-0934(02)00208-2
29 https://doi.org/10.1016/s0167-5877(03)00163-6
30 https://doi.org/10.1017/s0022029900021646
31 https://doi.org/10.1051/vetres:2005048
32 https://doi.org/10.1073/pnas.82.20.7086
33 https://doi.org/10.1097/00005072-195707000-00010
34 https://doi.org/10.1111/j.1439-0264.2006.00720.x
35 https://doi.org/10.1111/j.1751-0813.1983.tb02832.x
36 https://doi.org/10.1111/j.1751-0813.1986.tb02984.x
37 https://doi.org/10.1128/jvi.74.21.10096-10103.2000
38 https://doi.org/10.1136/vr.129.3.51
39 https://doi.org/10.1177/030098587100800108
40 https://doi.org/10.1177/030098589403100610
41 https://doi.org/10.1177/104063879901100507
42 https://doi.org/10.4081/849
43 schema:datePublished 2019-12
44 schema:datePublishedReg 2019-12-01
45 schema:description BACKGROUND: In order to characterize the complete range of lesions, especially minimal, affecting mammary gland and viral antigen distribution and target cells using immunohistochemistry in naturally Visna/maedi (VM) 84 infected sheep were studied, forty-four from flocks with clinical cases (A) and 35 randomly sampled from two abattoirs (B) together with five negative controls (C). An immunocytochemistry technique was developed and further milk samples (n = 39) were used to study viral excretion, carrier cells and the role of milk and colostrum in the transmission of the disease. RESULTS: All sheep from group C and three sheep from group B were negative to VM in tissue sections by histopathology, immunohistochemistry and PCR, and also in serum using ELISA. Several degrees of CD3 + lymphocytic interstitial mastitis were observed in groups A and B: minimal (+) n = 26 sheep; moderate (++), n = 32 and severe (+++), n = 12. No differences in lesion distribution were observed between groups A and B. Viral presence was confirmed by immunohistochemistry using two different antibodies and/or PCR in every tissue with lesions while serology was negative in six sheep with lesions. Two milk samples taken from milk tanks from two flocks from group A and fourteen milk samples from 29 infected sheep from group B were positive to VM (most of them from animals with moderate and severe lesions). Positivity was only found in macrophages, even in focal and minimal lesions, while no positivity was observed in epithelial or any other cells in either tissue and milk samples. CONCLUSIONS: This new observation of the minimal lesions described in this work increased the prevalence of VM lesions in mammary gland up to 90.9% and VM should be considered as a differential diagnosis when minimal interstitial lesions are detected. A high prevalence of VM was observed in intensive milk-producing sheep, ELISA serology did not detect as positivity all infected animals, while histology, IHC or PCR showed higher sensitivity. The cytological technique developed was very useful in milk-cell studies using hematoxylin and eosin and immunocytochemistry. Viral detection in milk samples (16/39) confirms a potential but limited role of milk/colostrum in viral transmission.
46 schema:genre research_article
47 schema:inLanguage en
48 schema:isAccessibleForFree true
49 schema:isPartOf N24b9984957ce41e3bcf71fb8d806c8e9
50 Nb25f06b9c44d488bbdfcc96065d19553
51 sg:journal.1035490
52 schema:name Characterization of minimal lesions related to the presence of visna/maedi virus in the mammary gland and milk of dairy sheep.
53 schema:pagination 109
54 schema:productId N5ae68903b6394dc9842a6b8d99c7dc44
55 N848b04646a7a4a57a66bf7bbaeb45abc
56 N852d4a08c95d4a0893738c66bde84d34
57 Nffd9dc54a697424baa4a54c51fbda69d
58 schema:sameAs https://app.dimensions.ai/details/publication/pub.1113327583
59 https://doi.org/10.1186/s12917-019-1855-3
60 schema:sdDatePublished 2019-04-15T09:13
61 schema:sdLicense https://scigraph.springernature.com/explorer/license/
62 schema:sdPublisher Ncd414bef6d0e4e3ab267654cf78a1fba
63 schema:url https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-019-1855-3
64 sgo:license sg:explorer/license/
65 sgo:sdDataset articles
66 rdf:type schema:ScholarlyArticle
67 N09115230f9dd47918ee533ba52499dbf rdf:first Ncf20274ceb0c496a884b945e28d253f8
68 rdf:rest Nb9b251c16ddc4c2481d3d9ee5479d48e
69 N09119e8bc4a54d7d8699b9ee84f862d8 rdf:first N59e761975aad44c798654d857d8396f4
70 rdf:rest N09115230f9dd47918ee533ba52499dbf
71 N1c3efef54e7443c5aff3a60a82d3d53c rdf:first Nf4e3cf73b9cd48d08f36c56509c2a0c8
72 rdf:rest Ne16409bdb3724dce95a130c8e0420c92
73 N24a02d8dcd5f42fcaf19879ad4d9a67c schema:affiliation https://www.grid.ac/institutes/grid.419063.9
74 schema:familyName Balseiro
75 schema:givenName A
76 rdf:type schema:Person
77 N24b9984957ce41e3bcf71fb8d806c8e9 schema:volumeNumber 15
78 rdf:type schema:PublicationVolume
79 N320c7cec99d24c3daf261b7d87c06351 schema:affiliation https://www.grid.ac/institutes/grid.4807.b
80 schema:familyName García Marín
81 schema:givenName J F
82 rdf:type schema:Person
83 N322edc18d33647338de5aec7bb7b5609 rdf:first Nf5f02dfa37764f43bbada423d3b12af8
84 rdf:rest Nac4d7829d2be4c028acfdc957ef1af96
85 N4e5fa79432d64a4ba8cb6e569544f457 schema:affiliation Nddcfb28e244e49879edb3c67e37bf618
86 schema:familyName Polledo
87 schema:givenName L
88 rdf:type schema:Person
89 N59e761975aad44c798654d857d8396f4 schema:affiliation https://www.grid.ac/institutes/grid.4807.b
90 schema:familyName García Iglesias
91 schema:givenName M J
92 rdf:type schema:Person
93 N5ae68903b6394dc9842a6b8d99c7dc44 schema:name doi
94 schema:value 10.1186/s12917-019-1855-3
95 rdf:type schema:PropertyValue
96 N5e5ea6b034784bd897934a7f4074710b rdf:first N320c7cec99d24c3daf261b7d87c06351
97 rdf:rest rdf:nil
98 N7fe38471d4fa405f93d02f8157de2a82 schema:affiliation https://www.grid.ac/institutes/grid.5602.1
99 schema:familyName Rossi
100 schema:givenName G
101 rdf:type schema:Person
102 N848b04646a7a4a57a66bf7bbaeb45abc schema:name nlm_unique_id
103 schema:value 101249759
104 rdf:type schema:PropertyValue
105 N852d4a08c95d4a0893738c66bde84d34 schema:name pubmed_id
106 schema:value 30967151
107 rdf:type schema:PropertyValue
108 Nac4d7829d2be4c028acfdc957ef1af96 rdf:first N24a02d8dcd5f42fcaf19879ad4d9a67c
109 rdf:rest N09119e8bc4a54d7d8699b9ee84f862d8
110 Nb25f06b9c44d488bbdfcc96065d19553 schema:issueNumber 1
111 rdf:type schema:PublicationIssue
112 Nb9b251c16ddc4c2481d3d9ee5479d48e rdf:first Ndf328287df8c47f1b0be6cf802e01377
113 rdf:rest Nd9eb5aa21aed4458a0dcdd7c052416e1
114 Ncd414bef6d0e4e3ab267654cf78a1fba schema:name Springer Nature - SN SciGraph project
115 rdf:type schema:Organization
116 Ncf20274ceb0c496a884b945e28d253f8 schema:affiliation https://www.grid.ac/institutes/grid.4807.b
117 schema:familyName Pérez Martínez
118 schema:givenName C
119 rdf:type schema:Person
120 Nd9eb5aa21aed4458a0dcdd7c052416e1 rdf:first N7fe38471d4fa405f93d02f8157de2a82
121 rdf:rest N5e5ea6b034784bd897934a7f4074710b
122 Nddcfb28e244e49879edb3c67e37bf618 schema:name Micros Veterinaria, León, Spain.
123 rdf:type schema:Organization
124 Ndf328287df8c47f1b0be6cf802e01377 schema:affiliation https://www.grid.ac/institutes/grid.5602.1
125 schema:familyName Preziuso
126 schema:givenName S
127 rdf:type schema:Person
128 Ne16409bdb3724dce95a130c8e0420c92 rdf:first N4e5fa79432d64a4ba8cb6e569544f457
129 rdf:rest N322edc18d33647338de5aec7bb7b5609
130 Nf4e3cf73b9cd48d08f36c56509c2a0c8 schema:affiliation https://www.grid.ac/institutes/grid.4807.b
131 schema:familyName Gayo
132 schema:givenName E
133 rdf:type schema:Person
134 Nf5f02dfa37764f43bbada423d3b12af8 schema:affiliation https://www.grid.ac/institutes/grid.4807.b
135 schema:familyName Magalde
136 schema:givenName A
137 rdf:type schema:Person
138 Nffd9dc54a697424baa4a54c51fbda69d schema:name dimensions_id
139 schema:value pub.1113327583
140 rdf:type schema:PropertyValue
141 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
142 schema:name Biological Sciences
143 rdf:type schema:DefinedTerm
144 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
145 schema:name Biochemistry and Cell Biology
146 rdf:type schema:DefinedTerm
147 sg:journal.1035490 schema:issn 1746-6148
148 schema:name BMC Veterinary Research
149 rdf:type schema:Periodical
150 sg:pub.10.1007/s13365-012-0131-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028540790
151 https://doi.org/10.1007/s13365-012-0131-0
152 rdf:type schema:CreativeWork
153 https://app.dimensions.ai/details/publication/pub.1074543048 schema:CreativeWork
154 https://app.dimensions.ai/details/publication/pub.1077178040 schema:CreativeWork
155 https://app.dimensions.ai/details/publication/pub.1081650294 schema:CreativeWork
156 https://app.dimensions.ai/details/publication/pub.1081656785 schema:CreativeWork
157 https://app.dimensions.ai/details/publication/pub.1082536152 schema:CreativeWork
158 https://app.dimensions.ai/details/publication/pub.1082788420 schema:CreativeWork
159 https://app.dimensions.ai/details/publication/pub.1083300362 schema:CreativeWork
160 https://doi.org/10.1016/0042-6822(85)90246-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044421010
161 rdf:type schema:CreativeWork
162 https://doi.org/10.1016/j.cimid.2011.12.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000161215
163 rdf:type schema:CreativeWork
164 https://doi.org/10.1016/j.jcpa.2011.09.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025781656
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1016/j.jcpa.2018.01.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101320428
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1016/j.jviromet.2006.07.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003160498
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1016/j.meegid.2010.06.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020638942
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1016/j.rvsc.2004.09.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045110516
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1016/j.rvsc.2005.05.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005854619
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1016/j.smallrumres.2012.12.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043531085
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1016/j.smallrumres.2017.10.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092317738
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1016/j.tvjl.2009.07.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001683902
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1016/j.tvjl.2013.03.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032859673
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1016/j.vetmic.2004.09.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014304851
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1016/j.vetmic.2005.01.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010971498
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/j.vetmic.2015.08.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028825469
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/s0034-5288(18)31108-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078999620
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/s0166-0934(02)00208-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008747170
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/s0167-5877(03)00163-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002380539
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1017/s0022029900021646 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053965614
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1051/vetres:2005048 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057072223
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1073/pnas.82.20.7086 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024802212
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1097/00005072-195707000-00010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045373292
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1111/j.1439-0264.2006.00720.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043724988
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1111/j.1751-0813.1983.tb02832.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1050211924
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1111/j.1751-0813.1986.tb02984.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022679922
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1128/jvi.74.21.10096-10103.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036795848
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1136/vr.129.3.51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062831063
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1177/030098587100800108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036211001
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1177/030098589403100610 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022680241
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1177/104063879901100507 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035129371
219 rdf:type schema:CreativeWork
220 https://doi.org/10.4081/849 schema:sameAs https://app.dimensions.ai/details/publication/pub.1076620712
221 rdf:type schema:CreativeWork
222 https://www.grid.ac/institutes/grid.419063.9 schema:alternateName Servicio Regional de Investigación y Desarrollo Agroalimentario
223 schema:name SERIDA, Gijón, Spain.
224 rdf:type schema:Organization
225 https://www.grid.ac/institutes/grid.4807.b schema:alternateName University of Leon
226 schema:name Veterinary Pathology Unit, School of Veterinary Medicine, University of León, León, Spain.
227 Veterinary Pathology Unit, School of Veterinary Medicine, University of León, León, Spain. egayr@unileon.es.
228 rdf:type schema:Organization
229 https://www.grid.ac/institutes/grid.5602.1 schema:alternateName University of Camerino
230 schema:name School of Bioscences and Veterinary Medicine, University of Camerino, Camerino, Italy.
231 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...