Long-term persistence of crAss-like phage crAss001 is associated with phase variation in Bacteroides intestinalis View Full Text


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Article Info

DATE

2021-08-18

AUTHORS

Andrey N. Shkoporov, Ekaterina V. Khokhlova, Niamh Stephens, Cara Hueston, Samuel Seymour, Andrew J. Hryckowian, Dimitri Scholz, R. Paul Ross, Colin Hill

ABSTRACT

BackgroundThe crAss-like phages are ubiquitous and highly abundant members of the human gut virome that infect commensal bacteria of the order Bacteroidales. Although incapable of lysogeny, these viruses demonstrate long-term persistence in the human gut microbiome, dominating the virome in some individuals.ResultsHere we show that rapid phase variation of alternate capsular polysaccharides in Bacteroides intestinalis cultures plays an important role in a dynamic equilibrium between phage sensitivity and resistance, allowing phage and bacteria to multiply in parallel. The data also suggests the role of a concomitant phage persistence mechanism associated with delayed lysis of infected cells, similar to carrier state infection. From an ecological and evolutionary standpoint, this type of phage-host interaction is consistent with the Piggyback-the-Winner model, which suggests a preference towards lysogenic or other “benign” forms of phage infection when the host is stably present at high abundance.ConclusionLong-term persistence of bacteriophage and host could result from mutually beneficial mechanisms driving bacterial strain-level diversity and phage survival in complex environments. More... »

PAGES

163

References to SciGraph publications

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  • 2018-11-14. ΦCrAss001 represents the most abundant bacteriophage family in the human gut and infects Bacteroides intestinalis in NATURE COMMUNICATIONS
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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12915-021-01084-3

    DOI

    http://dx.doi.org/10.1186/s12915-021-01084-3

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/34407825


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    29 schema:description BackgroundThe crAss-like phages are ubiquitous and highly abundant members of the human gut virome that infect commensal bacteria of the order Bacteroidales. Although incapable of lysogeny, these viruses demonstrate long-term persistence in the human gut microbiome, dominating the virome in some individuals.ResultsHere we show that rapid phase variation of alternate capsular polysaccharides in Bacteroides intestinalis cultures plays an important role in a dynamic equilibrium between phage sensitivity and resistance, allowing phage and bacteria to multiply in parallel. The data also suggests the role of a concomitant phage persistence mechanism associated with delayed lysis of infected cells, similar to carrier state infection. From an ecological and evolutionary standpoint, this type of phage-host interaction is consistent with the Piggyback-the-Winner model, which suggests a preference towards lysogenic or other “benign” forms of phage infection when the host is stably present at high abundance.ConclusionLong-term persistence of bacteriophage and host could result from mutually beneficial mechanisms driving bacterial strain-level diversity and phage survival in complex environments.
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    37 Bacteroides intestinalis
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    41 bacteria
    42 bacteriophages
    43 beneficial mechanisms
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    45 carrier state infection
    46 cells
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    48 complex environments
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