Production of high titer of citric acid from inulin View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Magdalena Rakicka, Jakub Wolniak, Zbigniew Lazar, Waldemar Rymowicz

ABSTRACT

BACKGROUND: Citric acid is considered as the most economically feasible product of microbiological production, therefore studies on cheap and renewable raw materials for its production are highly desirable. In this study citric acid was synthesized by genetically engineered strains of Yarrowia lipolytica from widely available, renewable polysaccharide - inulin. Hydrolysis of inulin by the Y. lipolytica strains was established by expressing the inulinase gene (INU1 gene; GenBank: X57202.1) with its native secretion signal sequence was amplified from genomic DNA from Kluyveromyces marxianus CBS6432. To ensure the maximum citric acid titer, the optimal cultivation strategy-repeated-batch culture was applied. RESULTS: The strain Y. lipolytica AWG7 INU 8 secreted more than 200 g dm- 3 of citric acid during repeated-batch culture on inulin, with a productivity of 0.51 g dm- 3 h- 1 and a yield of 0.85 g g- 1. CONCLUSIONS: The citric acid titer obtained in the proposed process is the highest value reported in the literature for Yarrowia yeast. The obtained results suggest that citric acid production from inulin by engineered Y. lipolytica may be a very promising technology for industrial citric acid production. More... »

PAGES

11

References to SciGraph publications

  • 2007-03. Inulinase production by a marine yeast Pichia guilliermondii and inulin hydrolysis by the crude inulinase in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2010-07. Citric acid production from glycerol-containing waste of biodiesel industry by Yarrowia lipolytica in batch, repeated batch, and cell recycle regimes in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2016-10. Cleaner production, Process Integration and intensification in CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY
  • 2005-09. Organic Acid Production by the Yeast Yarrowia lipolytica: A Review of Prospects in APPLIED BIOCHEMISTRY AND MICROBIOLOGY
  • 1988-10. The adjuvanticity of gamma inulin in IMMUNOLOGY AND CELL BIOLOGY
  • 2013-02. Both Decrease in ACL1 Gene Expression and Increase in ICL1 Gene Expression in Marine-Derived Yeast Yarrowia lipolytica Expressing INU1 Gene Enhance Citric Acid Production from Inulin in JOURNAL OF MARINE BIOTECHNOLOGY
  • 2017-12. Characterization of erythrose reductase from Yarrowia lipolytica and its influence on erythritol synthesis in MICROBIAL CELL FACTORIES
  • 2013-08. The citric acid production from raw glycerol by Yarrowia lipolytica yeast and its regulation in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2016-11. Efficient utilization of inulin and glycerol as fermentation substrates in erythritol and citric acid production using Yarrowia lipolytica expressing inulinase in CHEMICAL PAPERS
  • 2017-12. Substrates and oxygen dependent citric acid production by Yarrowia lipolytica: insights through transcriptome and fluxome analyses in MICROBIAL CELL FACTORIES
  • 2010-05. High-yield production of citric acid by Yarrowia lipolytica on glycerol in repeated-batch bioreactors in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2007-01. Potentiation of photodynamic therapy of cancer by complement: the effect of γ-inulin in BRITISH JOURNAL OF CANCER
  • 2009-03. Impact of the Crystallinity on the Physical Properties of Inulin during Water Sorption in FOOD BIOPHYSICS
  • 2016-02. Cloning and Characterization of a Pyruvate Carboxylase Gene from Penicillium rubens and Overexpression of the Genein the Yeast Yarrowia lipolytica for Enhanced Citric Acid Production in JOURNAL OF MARINE BIOTECHNOLOGY
  • 2017-12. Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica in BIOTECHNOLOGY FOR BIOFUELS
  • 2007-12. Overexpression of the ICL1 gene changes the product ratio of citric acid production by Yarrowia lipolytica in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2015-12. Transcriptional analysis of Kluyveromyces marxianus for ethanol production from inulin using consolidated bioprocessing technology in BIOTECHNOLOGY FOR BIOFUELS
  • 2010-07. Comparison of citric acid production from glycerol and glucose by different strains of Yarrowia lipolytica in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • Journal

    TITLE

    BMC Biotechnology

    ISSUE

    1

    VOLUME

    19

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12896-019-0503-0

    DOI

    http://dx.doi.org/10.1186/s12896-019-0503-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112062957

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30744615


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