Extended spectrum beta-lactamase and fluoroquinolone resistance genes among Escherichia coli and Salmonella isolates from children with diarrhea, Burkina Faso View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2020-10-03

AUTHORS

René Dembélé, Ali Konaté, Oumar Traoré, Wendpoulomdé A. D. Kaboré, Issiaka Soulama, Assèta Kagambèga, Alfred S. Traoré, Nathalie K. Guessennd, Awa Aidara-Kane, Amy Gassama-Sow, Nicolas Barro

ABSTRACT

BackgroundThe emergence and spread of multidrug-resistant gram-negative bacteria (MDR) has become a major public health concern worldwide. This resistance is caused by enzymes-mediated genes (i.e., extended spectrum beta-lactamases) that are common in certain Enterobacterioceae species. However, the distribution of these genes is poorly documented in Burkina Faso. This study aims to determine the prevalence and distribution of the resistant genes coding for broad spectrum beta-lactamases and quinolones in rural Burkina Faso.MethodsMultiplex PCR assays were carried out to detect ESBL-encoding genes, including blaOXA, blaTEM, blaCTX-M, blaSHV. The assays also assessed the presence of quinolone resistance gene namely qnrA, qnrB and qnrS in the quinolone-resistance DEC and Salmonella strains.ResultsThe Extended-Spectrum Beta-Lactamases (ESBL) resistance phenotype was reported in all the E. coli isolates (5/5). Cross-resistance phenotype to quinolones (CRQ) was shown by one Salmonella strain (1/9) and three E. coli (3/5). Cross-resistance phenotypes to fluoroquinolones (CRFQ) were harboured by one Salmonella (1/9) and carbapenemase phenotypes were detected in two E. coli strains (2/5). Whilst the blaOXA genes were detected in 100% (5/5) of E. coli isolates and in 33.33% (3/9) Salmonella isolates. One strain of E. coli (1/5) harbored the blaCTX−M gene and the qnrB gene simultaneously.ConclusionsThis study identified β-lactam (bla) and quinolone resistance (qnr) genes in multidrug-resistant E. coli and Salmonella spp. in rural Burkina Faso. Our finding which highlighted the enterobacteriaceae strains resistance to β-lactams and quinolones are of high interest for adequate management of antimicrobial resistant genes outbreak in Burkina Faso. More... »

PAGES

459

References to SciGraph publications

  • 2018-07-13. Distribution of resistance genes encoding ESBLs in Enterobacteriaceae isolated from biological samples in health centers in Ouagadougou, Burkina Faso in BMC RESEARCH NOTES
  • 2009-02-24. New 16-plex PCR method for rapid detection of diarrheagenic Escherichia coli directly from stool samples in EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
  • 2019-10-31. Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad in ANTIMICROBIAL RESISTANCE & INFECTION CONTROL
  • 2016-07-11. High prevalence of extended-spectrum ß-lactamase producing enterobacteriaceae among clinical isolates in Burkina Faso in BMC INFECTIOUS DISEASES
  • 2018-07-25. Prevalence and antibiotic susceptibility patterns of Shigella and Salmonella among children aged below five years with Diarrhoea attending Nigist Eleni Mohammed memorial hospital, South Ethiopia in BMC PEDIATRICS
  • 2013-03-19. Bacterial and viral etiology of childhood diarrhea in Ouagadougou, Burkina Faso in BMC PEDIATRICS
  • 2013-11-11. Prevalence and characterization of Salmonella enterica from the feces of cattle, poultry, swine and hedgehogs in Burkina Faso and their comparison to human Salmonellaisolates in BMC MICROBIOLOGY
  • 2019-06-18. Distribution of quinolone resistance gene (qnr) in ESBL-producing Escherichia coli and Klebsiella spp. in Lomé, Togo in ANTIMICROBIAL RESISTANCE & INFECTION CONTROL
  • 2007-08-09. Identification of diarrheagenic Escherichia coli isolated from infants and children in Dar es Salaam, Tanzania in BMC INFECTIOUS DISEASES
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12887-020-02342-z

    DOI

    http://dx.doi.org/10.1186/s12887-020-02342-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1131419600

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/33010801


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Anti-Bacterial Agents", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Burkina Faso", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Child", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Diarrhea", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli Infections", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fluoroquinolones", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Microbial Sensitivity Tests", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Salmonella", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "beta-Lactamases", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Training and Research Unit in Applied Sciences and Technologies, University of Dedougou, BP 176, Dedougou, Burkina Faso", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso", 
                "Training and Research Unit in Applied Sciences and Technologies, University of Dedougou, BP 176, Dedougou, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Demb\u00e9l\u00e9", 
            "givenName": "Ren\u00e9", 
            "id": "sg:person.012503231743.69", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012503231743.69"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Konat\u00e9", 
            "givenName": "Ali", 
            "id": "sg:person.011705651343.17", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011705651343.17"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Training and Research Unit in Applied Sciences and Technologies, University of Dedougou, BP 176, Dedougou, Burkina Faso", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso", 
                "Training and Research Unit in Applied Sciences and Technologies, University of Dedougou, BP 176, Dedougou, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Traor\u00e9", 
            "givenName": "Oumar", 
            "id": "sg:person.01307563033.60", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307563033.60"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kabor\u00e9", 
            "givenName": "Wendpoulomd\u00e9 A. D.", 
            "id": "sg:person.016373153433.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016373153433.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Centre for Research and Training on Malaria, 01, BP 2208, Ouagadougou 01, Burkina Faso", 
              "id": "http://www.grid.ac/institutes/grid.507461.1", 
              "name": [
                "National Centre for Research and Training on Malaria, 01, BP 2208, Ouagadougou 01, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Soulama", 
            "givenName": "Issiaka", 
            "id": "sg:person.01017366704.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01017366704.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Sciences, 01, BP 1757, Ouagadougou 01, Burkina Faso", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso", 
                "Institute of Sciences, 01, BP 1757, Ouagadougou 01, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kagamb\u00e8ga", 
            "givenName": "Ass\u00e8ta", 
            "id": "sg:person.01363220233.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363220233.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Traor\u00e9", 
            "givenName": "Alfred S.", 
            "id": "sg:person.016466717302.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016466717302.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratory of Bacteriology-Virology, Unit of Training and Research of Medical Sciences, University Felix Houphouet BOIGNY, 01, BP V34, Abidjan 01, Ivory Coast", 
              "id": "http://www.grid.ac/institutes/grid.410694.e", 
              "name": [
                "Laboratory of Bacteriology-Virology, Unit of Antibiotics, Natural Substances and Surveillance of Resistance of Microorganisms to Antimicrobials, Pasteur Institute of Abidjan, 01, BP 490, Abidjan 01, Ivory Coast", 
                "Laboratory of Bacteriology-Virology, Unit of Training and Research of Medical Sciences, University Felix Houphouet BOIGNY, 01, BP V34, Abidjan 01, Ivory Coast"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Guessennd", 
            "givenName": "Nathalie K.", 
            "id": "sg:person.01051267675.94", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051267675.94"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Unit of Experimental Bacteriology, Pasteur Institute of Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal", 
              "id": "http://www.grid.ac/institutes/grid.418508.0", 
              "name": [
                "Unit of Experimental Bacteriology, Pasteur Institute of Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Aidara-Kane", 
            "givenName": "Awa", 
            "id": "sg:person.0713142741.17", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0713142741.17"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Unit of Experimental Bacteriology, Pasteur Institute of Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal", 
              "id": "http://www.grid.ac/institutes/grid.418508.0", 
              "name": [
                "Unit of Experimental Bacteriology, Pasteur Institute of Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gassama-Sow", 
            "givenName": "Amy", 
            "id": "sg:person.01360210612.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01360210612.53"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Barro", 
            "givenName": "Nicolas", 
            "id": "sg:person.0710707132.87", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0710707132.87"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1186/1471-2431-13-36", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002370193", 
              "https://doi.org/10.1186/1471-2431-13-36"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12887-018-1221-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1105837190", 
              "https://doi.org/10.1186/s12887-018-1221-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2180-13-253", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020325587", 
              "https://doi.org/10.1186/1471-2180-13-253"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13756-019-0552-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1117329189", 
              "https://doi.org/10.1186/s13756-019-0552-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13104-018-3581-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1105566381", 
              "https://doi.org/10.1186/s13104-018-3581-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10096-009-0720-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017026114", 
              "https://doi.org/10.1007/s10096-009-0720-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13756-019-0626-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1122238944", 
              "https://doi.org/10.1186/s13756-019-0626-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12879-016-1655-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006168993", 
              "https://doi.org/10.1186/s12879-016-1655-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2334-7-92", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053471925", 
              "https://doi.org/10.1186/1471-2334-7-92"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2020-10-03", 
        "datePublishedReg": "2020-10-03", 
        "description": "BackgroundThe emergence and spread of multidrug-resistant gram-negative bacteria (MDR) has become a major public health concern worldwide. This resistance is caused by enzymes-mediated genes (i.e., extended spectrum beta-lactamases) that are common in certain Enterobacterioceae species. However, the distribution of these genes is poorly documented in Burkina Faso. This study aims to determine the prevalence and distribution of the resistant genes coding for broad spectrum beta-lactamases and quinolones in rural Burkina Faso.MethodsMultiplex PCR assays were carried out to detect ESBL-encoding genes, including blaOXA, blaTEM, blaCTX-M, blaSHV. The assays also assessed the presence of quinolone resistance gene namely qnrA, qnrB and qnrS in the quinolone-resistance DEC and Salmonella strains.ResultsThe Extended-Spectrum Beta-Lactamases (ESBL) resistance phenotype was reported in all the E. coli isolates (5/5). Cross-resistance phenotype to quinolones (CRQ) was shown by one Salmonella strain (1/9) and three E. coli (3/5). Cross-resistance phenotypes to fluoroquinolones (CRFQ) were harboured by one Salmonella (1/9) and carbapenemase phenotypes were detected in two E. coli strains (2/5). Whilst the blaOXA genes were detected in 100% (5/5) of E. coli isolates and in 33.33% (3/9) Salmonella isolates. One strain of E. coli (1/5) harbored the blaCTX\u2212M gene and the qnrB gene simultaneously.ConclusionsThis study identified \u03b2-lactam (bla) and quinolone resistance (qnr) genes in multidrug-resistant E. coli and Salmonella spp. in rural Burkina Faso. Our finding which highlighted the enterobacteriaceae strains resistance to \u03b2-lactams and quinolones are of high interest for adequate management of antimicrobial resistant genes outbreak in Burkina Faso.", 
        "genre": "article", 
        "id": "sg:pub.10.1186/s12887-020-02342-z", 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1024635", 
            "issn": [
              "1471-2431"
            ], 
            "name": "BMC Pediatrics", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "20"
          }
        ], 
        "keywords": [
          "rural Burkina Faso", 
          "quinolone resistance genes", 
          "cross-resistance phenotype", 
          "major public health concern", 
          "multidrug-resistant E. coli", 
          "public health concern", 
          "ESBL-encoding genes", 
          "E. coli isolates", 
          "\u03b2-lactams", 
          "Burkina Faso", 
          "qnrB genes", 
          "extended spectrum", 
          "carbapenemase phenotypes", 
          "health concern", 
          "fluoroquinolone resistance genes", 
          "blaOXA genes", 
          "ConclusionsThis study", 
          "coli isolates", 
          "Salmonella strains", 
          "adequate management", 
          "E. coli", 
          "quinolones", 
          "E. coli strains", 
          "resistance phenotype", 
          "PCR assays", 
          "Salmonella spp", 
          "Salmonella", 
          "phenotype", 
          "resistance genes", 
          "negative bacteria", 
          "broad spectrum", 
          "coli strains", 
          "assays", 
          "diarrhea", 
          "resistant genes", 
          "genes", 
          "prevalence", 
          "Faso", 
          "blaOXA", 
          "blaSHV", 
          "qnrA", 
          "qnrB", 
          "blaTEM", 
          "ResultsThe", 
          "fluoroquinolones", 
          "blaCTX", 
          "qnrS", 
          "children", 
          "Enterobacteriaceae", 
          "study", 
          "strains", 
          "outbreak", 
          "coli", 
          "resistance", 
          "isolates", 
          "Escherichia coli", 
          "findings", 
          "management", 
          "spread", 
          "presence", 
          "spp", 
          "bacteria", 
          "concern", 
          "high interest", 
          "emergence", 
          "distribution", 
          "species", 
          "interest", 
          "spectra"
        ], 
        "name": "Extended spectrum beta-lactamase and fluoroquinolone resistance genes among Escherichia coli and Salmonella isolates from children with diarrhea, Burkina Faso", 
        "pagination": "459", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1131419600"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s12887-020-02342-z"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "33010801"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s12887-020-02342-z", 
          "https://app.dimensions.ai/details/publication/pub.1131419600"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-11-24T21:05", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_857.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1186/s12887-020-02342-z"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12887-020-02342-z'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12887-020-02342-z'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12887-020-02342-z'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12887-020-02342-z'


     

    This table displays all metadata directly associated to this object as RDF triples.

    293 TRIPLES      21 PREDICATES      114 URIs      97 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s12887-020-02342-z schema:about N04bbf5b1b8ff4683bd9cf7a51891cac8
    2 N15f0a1bb3d1e4e6cb520c8951fd05424
    3 N2a02737c5faa42cc8be0cc36658e71ec
    4 N3caf20f010be49bf9a42a5e0d542c2bb
    5 N4f2c1afb0d784aafbea889f54e7903eb
    6 N617bc72bf3934cd9bb77688538fbabda
    7 N66a210c30e814db7be0a3edd66e89b26
    8 N7c984d02540e42509d8fa84eb6da3f5f
    9 N8e6baf5470a44aeabcb04ba17466551a
    10 N9218ebcf79fe4a58bb237ed6eb19b190
    11 Nc9572b0854b74828b45681f441df8d72
    12 anzsrc-for:11
    13 anzsrc-for:1108
    14 schema:author Nf3eb52a7145347b78516763e90f04ca1
    15 schema:citation sg:pub.10.1007/s10096-009-0720-x
    16 sg:pub.10.1186/1471-2180-13-253
    17 sg:pub.10.1186/1471-2334-7-92
    18 sg:pub.10.1186/1471-2431-13-36
    19 sg:pub.10.1186/s12879-016-1655-3
    20 sg:pub.10.1186/s12887-018-1221-9
    21 sg:pub.10.1186/s13104-018-3581-5
    22 sg:pub.10.1186/s13756-019-0552-0
    23 sg:pub.10.1186/s13756-019-0626-z
    24 schema:datePublished 2020-10-03
    25 schema:datePublishedReg 2020-10-03
    26 schema:description BackgroundThe emergence and spread of multidrug-resistant gram-negative bacteria (MDR) has become a major public health concern worldwide. This resistance is caused by enzymes-mediated genes (i.e., extended spectrum beta-lactamases) that are common in certain Enterobacterioceae species. However, the distribution of these genes is poorly documented in Burkina Faso. This study aims to determine the prevalence and distribution of the resistant genes coding for broad spectrum beta-lactamases and quinolones in rural Burkina Faso.MethodsMultiplex PCR assays were carried out to detect ESBL-encoding genes, including blaOXA, blaTEM, blaCTX-M, blaSHV. The assays also assessed the presence of quinolone resistance gene namely qnrA, qnrB and qnrS in the quinolone-resistance DEC and Salmonella strains.ResultsThe Extended-Spectrum Beta-Lactamases (ESBL) resistance phenotype was reported in all the E. coli isolates (5/5). Cross-resistance phenotype to quinolones (CRQ) was shown by one Salmonella strain (1/9) and three E. coli (3/5). Cross-resistance phenotypes to fluoroquinolones (CRFQ) were harboured by one Salmonella (1/9) and carbapenemase phenotypes were detected in two E. coli strains (2/5). Whilst the blaOXA genes were detected in 100% (5/5) of E. coli isolates and in 33.33% (3/9) Salmonella isolates. One strain of E. coli (1/5) harbored the blaCTX−M gene and the qnrB gene simultaneously.ConclusionsThis study identified β-lactam (bla) and quinolone resistance (qnr) genes in multidrug-resistant E. coli and Salmonella spp. in rural Burkina Faso. Our finding which highlighted the enterobacteriaceae strains resistance to β-lactams and quinolones are of high interest for adequate management of antimicrobial resistant genes outbreak in Burkina Faso.
    27 schema:genre article
    28 schema:isAccessibleForFree true
    29 schema:isPartOf N42d1916833e241648d76e23d4ce3233f
    30 N934866256c82445cb8100a7ac7062b90
    31 sg:journal.1024635
    32 schema:keywords Burkina Faso
    33 ConclusionsThis study
    34 E. coli
    35 E. coli isolates
    36 E. coli strains
    37 ESBL-encoding genes
    38 Enterobacteriaceae
    39 Escherichia coli
    40 Faso
    41 PCR assays
    42 ResultsThe
    43 Salmonella
    44 Salmonella spp
    45 Salmonella strains
    46 adequate management
    47 assays
    48 bacteria
    49 blaCTX
    50 blaOXA
    51 blaOXA genes
    52 blaSHV
    53 blaTEM
    54 broad spectrum
    55 carbapenemase phenotypes
    56 children
    57 coli
    58 coli isolates
    59 coli strains
    60 concern
    61 cross-resistance phenotype
    62 diarrhea
    63 distribution
    64 emergence
    65 extended spectrum
    66 findings
    67 fluoroquinolone resistance genes
    68 fluoroquinolones
    69 genes
    70 health concern
    71 high interest
    72 interest
    73 isolates
    74 major public health concern
    75 management
    76 multidrug-resistant E. coli
    77 negative bacteria
    78 outbreak
    79 phenotype
    80 presence
    81 prevalence
    82 public health concern
    83 qnrA
    84 qnrB
    85 qnrB genes
    86 qnrS
    87 quinolone resistance genes
    88 quinolones
    89 resistance
    90 resistance genes
    91 resistance phenotype
    92 resistant genes
    93 rural Burkina Faso
    94 species
    95 spectra
    96 spp
    97 spread
    98 strains
    99 study
    100 β-lactams
    101 schema:name Extended spectrum beta-lactamase and fluoroquinolone resistance genes among Escherichia coli and Salmonella isolates from children with diarrhea, Burkina Faso
    102 schema:pagination 459
    103 schema:productId Na8ebe35c3dc643eea7f2a4c748c3a448
    104 Nd5b2d35cb56f4e2f9136dc9401e1dfb7
    105 Nf47b1555286348779e11ccd58f020fa1
    106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1131419600
    107 https://doi.org/10.1186/s12887-020-02342-z
    108 schema:sdDatePublished 2022-11-24T21:05
    109 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    110 schema:sdPublisher N20cef33e5d4948b6a5747962d603c71e
    111 schema:url https://doi.org/10.1186/s12887-020-02342-z
    112 sgo:license sg:explorer/license/
    113 sgo:sdDataset articles
    114 rdf:type schema:ScholarlyArticle
    115 N04bbf5b1b8ff4683bd9cf7a51891cac8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Salmonella
    117 rdf:type schema:DefinedTerm
    118 N0c803d4bc433456486c2aa1bbd628604 rdf:first sg:person.016373153433.30
    119 rdf:rest N1d24a6f31d134d8480ef740ff9334204
    120 N15f0a1bb3d1e4e6cb520c8951fd05424 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Burkina Faso
    122 rdf:type schema:DefinedTerm
    123 N1d24a6f31d134d8480ef740ff9334204 rdf:first sg:person.01017366704.04
    124 rdf:rest N1dd6e76868014a9499c7ff595f5a8f6c
    125 N1dd6e76868014a9499c7ff595f5a8f6c rdf:first sg:person.01363220233.32
    126 rdf:rest N766fe130c63d41b2a909ff15650da85f
    127 N20cef33e5d4948b6a5747962d603c71e schema:name Springer Nature - SN SciGraph project
    128 rdf:type schema:Organization
    129 N2a02737c5faa42cc8be0cc36658e71ec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Fluoroquinolones
    131 rdf:type schema:DefinedTerm
    132 N2f7ffbe4e85441248965c02ed5d7c8e5 rdf:first sg:person.01051267675.94
    133 rdf:rest Nbac998f673b54d0eb11dbc0ea0edf2c2
    134 N348a5c6e0550464d9956d6243f19d0c6 rdf:first sg:person.011705651343.17
    135 rdf:rest N40a96ee069254986a5d0d8ca1974cd68
    136 N3caf20f010be49bf9a42a5e0d542c2bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Escherichia coli Infections
    138 rdf:type schema:DefinedTerm
    139 N40a96ee069254986a5d0d8ca1974cd68 rdf:first sg:person.01307563033.60
    140 rdf:rest N0c803d4bc433456486c2aa1bbd628604
    141 N42d1916833e241648d76e23d4ce3233f schema:issueNumber 1
    142 rdf:type schema:PublicationIssue
    143 N4f2c1afb0d784aafbea889f54e7903eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Humans
    145 rdf:type schema:DefinedTerm
    146 N617bc72bf3934cd9bb77688538fbabda schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name beta-Lactamases
    148 rdf:type schema:DefinedTerm
    149 N66a210c30e814db7be0a3edd66e89b26 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Diarrhea
    151 rdf:type schema:DefinedTerm
    152 N766fe130c63d41b2a909ff15650da85f rdf:first sg:person.016466717302.31
    153 rdf:rest N2f7ffbe4e85441248965c02ed5d7c8e5
    154 N7c984d02540e42509d8fa84eb6da3f5f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    155 schema:name Escherichia coli
    156 rdf:type schema:DefinedTerm
    157 N8e6baf5470a44aeabcb04ba17466551a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    158 schema:name Anti-Bacterial Agents
    159 rdf:type schema:DefinedTerm
    160 N9218ebcf79fe4a58bb237ed6eb19b190 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name Child
    162 rdf:type schema:DefinedTerm
    163 N934866256c82445cb8100a7ac7062b90 schema:volumeNumber 20
    164 rdf:type schema:PublicationVolume
    165 Na5d0c79f99d24f70af712d6137a08771 rdf:first sg:person.01360210612.53
    166 rdf:rest Ndaa9421cc43947f9bcf3ef8fab1fb2b9
    167 Na8ebe35c3dc643eea7f2a4c748c3a448 schema:name pubmed_id
    168 schema:value 33010801
    169 rdf:type schema:PropertyValue
    170 Nbac998f673b54d0eb11dbc0ea0edf2c2 rdf:first sg:person.0713142741.17
    171 rdf:rest Na5d0c79f99d24f70af712d6137a08771
    172 Nc9572b0854b74828b45681f441df8d72 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    173 schema:name Microbial Sensitivity Tests
    174 rdf:type schema:DefinedTerm
    175 Nd5b2d35cb56f4e2f9136dc9401e1dfb7 schema:name dimensions_id
    176 schema:value pub.1131419600
    177 rdf:type schema:PropertyValue
    178 Ndaa9421cc43947f9bcf3ef8fab1fb2b9 rdf:first sg:person.0710707132.87
    179 rdf:rest rdf:nil
    180 Nf3eb52a7145347b78516763e90f04ca1 rdf:first sg:person.012503231743.69
    181 rdf:rest N348a5c6e0550464d9956d6243f19d0c6
    182 Nf47b1555286348779e11ccd58f020fa1 schema:name doi
    183 schema:value 10.1186/s12887-020-02342-z
    184 rdf:type schema:PropertyValue
    185 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    186 schema:name Medical and Health Sciences
    187 rdf:type schema:DefinedTerm
    188 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
    189 schema:name Medical Microbiology
    190 rdf:type schema:DefinedTerm
    191 sg:journal.1024635 schema:issn 1471-2431
    192 schema:name BMC Pediatrics
    193 schema:publisher Springer Nature
    194 rdf:type schema:Periodical
    195 sg:person.01017366704.04 schema:affiliation grid-institutes:grid.507461.1
    196 schema:familyName Soulama
    197 schema:givenName Issiaka
    198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01017366704.04
    199 rdf:type schema:Person
    200 sg:person.01051267675.94 schema:affiliation grid-institutes:grid.410694.e
    201 schema:familyName Guessennd
    202 schema:givenName Nathalie K.
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051267675.94
    204 rdf:type schema:Person
    205 sg:person.011705651343.17 schema:affiliation grid-institutes:None
    206 schema:familyName Konaté
    207 schema:givenName Ali
    208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011705651343.17
    209 rdf:type schema:Person
    210 sg:person.012503231743.69 schema:affiliation grid-institutes:None
    211 schema:familyName Dembélé
    212 schema:givenName René
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012503231743.69
    214 rdf:type schema:Person
    215 sg:person.01307563033.60 schema:affiliation grid-institutes:None
    216 schema:familyName Traoré
    217 schema:givenName Oumar
    218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307563033.60
    219 rdf:type schema:Person
    220 sg:person.01360210612.53 schema:affiliation grid-institutes:grid.418508.0
    221 schema:familyName Gassama-Sow
    222 schema:givenName Amy
    223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01360210612.53
    224 rdf:type schema:Person
    225 sg:person.01363220233.32 schema:affiliation grid-institutes:None
    226 schema:familyName Kagambèga
    227 schema:givenName Assèta
    228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363220233.32
    229 rdf:type schema:Person
    230 sg:person.016373153433.30 schema:affiliation grid-institutes:None
    231 schema:familyName Kaboré
    232 schema:givenName Wendpoulomdé A. D.
    233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016373153433.30
    234 rdf:type schema:Person
    235 sg:person.016466717302.31 schema:affiliation grid-institutes:None
    236 schema:familyName Traoré
    237 schema:givenName Alfred S.
    238 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016466717302.31
    239 rdf:type schema:Person
    240 sg:person.0710707132.87 schema:affiliation grid-institutes:None
    241 schema:familyName Barro
    242 schema:givenName Nicolas
    243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0710707132.87
    244 rdf:type schema:Person
    245 sg:person.0713142741.17 schema:affiliation grid-institutes:grid.418508.0
    246 schema:familyName Aidara-Kane
    247 schema:givenName Awa
    248 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0713142741.17
    249 rdf:type schema:Person
    250 sg:pub.10.1007/s10096-009-0720-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017026114
    251 https://doi.org/10.1007/s10096-009-0720-x
    252 rdf:type schema:CreativeWork
    253 sg:pub.10.1186/1471-2180-13-253 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020325587
    254 https://doi.org/10.1186/1471-2180-13-253
    255 rdf:type schema:CreativeWork
    256 sg:pub.10.1186/1471-2334-7-92 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053471925
    257 https://doi.org/10.1186/1471-2334-7-92
    258 rdf:type schema:CreativeWork
    259 sg:pub.10.1186/1471-2431-13-36 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002370193
    260 https://doi.org/10.1186/1471-2431-13-36
    261 rdf:type schema:CreativeWork
    262 sg:pub.10.1186/s12879-016-1655-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006168993
    263 https://doi.org/10.1186/s12879-016-1655-3
    264 rdf:type schema:CreativeWork
    265 sg:pub.10.1186/s12887-018-1221-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105837190
    266 https://doi.org/10.1186/s12887-018-1221-9
    267 rdf:type schema:CreativeWork
    268 sg:pub.10.1186/s13104-018-3581-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105566381
    269 https://doi.org/10.1186/s13104-018-3581-5
    270 rdf:type schema:CreativeWork
    271 sg:pub.10.1186/s13756-019-0552-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1117329189
    272 https://doi.org/10.1186/s13756-019-0552-0
    273 rdf:type schema:CreativeWork
    274 sg:pub.10.1186/s13756-019-0626-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1122238944
    275 https://doi.org/10.1186/s13756-019-0626-z
    276 rdf:type schema:CreativeWork
    277 grid-institutes:None schema:alternateName Institute of Sciences, 01, BP 1757, Ouagadougou 01, Burkina Faso
    278 Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso
    279 Training and Research Unit in Applied Sciences and Technologies, University of Dedougou, BP 176, Dedougou, Burkina Faso
    280 schema:name Institute of Sciences, 01, BP 1757, Ouagadougou 01, Burkina Faso
    281 Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Science and Technology, University Joseph KI-ZERBO, 03, BP 7021, Ouagadougou 03, Burkina Faso
    282 Training and Research Unit in Applied Sciences and Technologies, University of Dedougou, BP 176, Dedougou, Burkina Faso
    283 rdf:type schema:Organization
    284 grid-institutes:grid.410694.e schema:alternateName Laboratory of Bacteriology-Virology, Unit of Training and Research of Medical Sciences, University Felix Houphouet BOIGNY, 01, BP V34, Abidjan 01, Ivory Coast
    285 schema:name Laboratory of Bacteriology-Virology, Unit of Antibiotics, Natural Substances and Surveillance of Resistance of Microorganisms to Antimicrobials, Pasteur Institute of Abidjan, 01, BP 490, Abidjan 01, Ivory Coast
    286 Laboratory of Bacteriology-Virology, Unit of Training and Research of Medical Sciences, University Felix Houphouet BOIGNY, 01, BP V34, Abidjan 01, Ivory Coast
    287 rdf:type schema:Organization
    288 grid-institutes:grid.418508.0 schema:alternateName Unit of Experimental Bacteriology, Pasteur Institute of Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal
    289 schema:name Unit of Experimental Bacteriology, Pasteur Institute of Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal
    290 rdf:type schema:Organization
    291 grid-institutes:grid.507461.1 schema:alternateName National Centre for Research and Training on Malaria, 01, BP 2208, Ouagadougou 01, Burkina Faso
    292 schema:name National Centre for Research and Training on Malaria, 01, BP 2208, Ouagadougou 01, Burkina Faso
    293 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...