LncRNA ENST00000539653 acts as an oncogenic factor via MAPK signalling in papillary thyroid cancer View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Bin Song, Rurun Li, Zhihua Zuo, Juan Tan, Ling Liu, Dafa Ding, Yibing Lu, Dawei Hou

ABSTRACT

BACKGROUND: Papillary thyroid cancer (PTC) is the most frequent type of thyroid malignancy. In this study, we investigated the mechanisms whereby long non-coding RNAs (lncRNAs) are associated with PTC pathogenesis. METHODS: Microarray analysis was used to determine differentially expressed lncRNAs between paired PTC tissues and normal adjacent thyroid tissues. Quantitative RT-PCR was used for validation in 86 PTC cases. Small interfering RNA (siRNA) transfection assays were then performed to assess how a novel lncRNA affected key proliferation and cell death pathways in IHH4 PTC cells. RESULTS: We identified 1878 differentially expressed lncRNAs versus matched control samples (fold change ≥2.0, P < 0.05), of which 429 were upregulated and 1449 were downregulated. ENST00000539653.1 (ENS-653), one of the top hits in this microarray, was selected for further study. Higher ENS-653 expression was observed in PTC tissue samples versus adjacent normal tissues, and was associated with a larger tumor size and a more advanced clinical stage. In the Cancer Genome Atlas (TCGA) PTC cohort, higher ENS-653 expression was correlated with more frequent BRAF (V600E) mutation and poorer disease-free survival. Furthermore, ENS-653 downregulation reduced the proliferation of PTC cells and led to G1-S arrest, but had no impact on apoptosis. ENS-653 downregulation also inactivated ERK1/2 and ERK5, causing partial MAPK cascade suppression. CONCLUSION: ENS-653 exhibits oncogenic properties in PTC, and could be a diagnostic and/or prognostic PTC biomarker, in addition to possibly being a future target for therapy. More... »

PAGES

297

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12885-019-5533-4

DOI

http://dx.doi.org/10.1186/s12885-019-5533-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1113179463

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30940124


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Yangzhou University", 
          "id": "https://www.grid.ac/institutes/grid.268415.c", 
          "name": [
            "Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, 210011, Nanjing, China", 
            "Department of Endocrinology, Clinical Medical College, Yangzhou University, 98 Nantong West Road, 225001, Yangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Song", 
        "givenName": "Bin", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Affiliated Hospital of Nanjing Medical University", 
          "id": "https://www.grid.ac/institutes/grid.452511.6", 
          "name": [
            "Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, 210011, Nanjing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Rurun", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Affiliated Hospital of Nanjing Medical University", 
          "id": "https://www.grid.ac/institutes/grid.452511.6", 
          "name": [
            "Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, 210011, Nanjing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zuo", 
        "givenName": "Zhihua", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nanjing Medical University", 
          "id": "https://www.grid.ac/institutes/grid.89957.3a", 
          "name": [
            "Department of Gerontology, Huai\u2019an First People\u2019s Hospital, Nanjing Medical University, 6 Beijing West Road, 223300, Huai\u2019an, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tan", 
        "givenName": "Juan", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Affiliated Hospital of Nanjing Medical University", 
          "id": "https://www.grid.ac/institutes/grid.452511.6", 
          "name": [
            "Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, 210011, Nanjing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Ling", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Affiliated Hospital of Nanjing Medical University", 
          "id": "https://www.grid.ac/institutes/grid.452511.6", 
          "name": [
            "Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, 210011, Nanjing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ding", 
        "givenName": "Dafa", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Affiliated Hospital of Nanjing Medical University", 
          "id": "https://www.grid.ac/institutes/grid.452511.6", 
          "name": [
            "Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, 210011, Nanjing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lu", 
        "givenName": "Yibing", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Affiliated Hospital of Nanjing Medical University", 
          "id": "https://www.grid.ac/institutes/grid.452511.6", 
          "name": [
            "Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, 210011, Nanjing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hou", 
        "givenName": "Dawei", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1210/jc.2016-1991", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000606849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1210/jc.2016-1991", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000606849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biopha.2016.05.039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000785264"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12032-016-0816-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004992572", 
          "https://doi.org/10.1007/s12032-016-0816-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12032-016-0816-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004992572", 
          "https://doi.org/10.1007/s12032-016-0816-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12032-016-0816-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004992572", 
          "https://doi.org/10.1007/s12032-016-0816-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2014.09.050", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006907054"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2014.09.050", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006907054"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/thy.2015.0020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007626247"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc3431", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009089021", 
          "https://doi.org/10.1038/nrc3431"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/febs.13741", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010308503"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3322/caac.21338", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010436755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3322/caac.21254", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011682275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000438637", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012476927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000438637", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012476927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1586/14737140.9.3.317", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013484119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s13277-015-4149-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013839346", 
          "https://doi.org/10.1007/s13277-015-4149-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ccell.2016.03.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017662736"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.18632/oncotarget.10825", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018898154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.18632/oncotarget.10825", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018898154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.18632/oncotarget.13725", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021943990"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.18632/oncotarget.13725", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021943990"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.18632/oncotarget.11087", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027736775"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/bmj.g3045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028650298"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/bmj.g3045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028650298"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(13)60109-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030980548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(13)60109-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030980548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmra1501993", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044889611"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmra1501993", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044889611"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1620/tjem.241.29", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050757498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-13-3440", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051068625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.18632/oncotarget.7828", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053537886"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/15384101.2016.1261768", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058402464"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/15384101.2016.1261768", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058402464"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/thy.2015.0193", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059322057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/thy.2015.0193", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059322057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/hmg/ddw056", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059645840"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1210/jc.2015-2247", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064295867"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/ijo.2016.3803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071514638"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4143/crt.2015.118", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072255994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12943-017-0588-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083844984", 
          "https://doi.org/10.1186/s12943-017-0588-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12943-017-0588-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083844984", 
          "https://doi.org/10.1186/s12943-017-0588-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.18632/oncotarget.15628", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084372776"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0176287", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085021725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-01957-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085330966", 
          "https://doi.org/10.1038/s41598-017-01957-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.26142", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085432454"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.26142", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085432454"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1530/erc-17-0188", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092737717"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12943-017-0756-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100381828", 
          "https://doi.org/10.1186/s12943-017-0756-y"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "BACKGROUND: Papillary thyroid cancer (PTC) is the most frequent type of thyroid malignancy. In this study, we investigated the mechanisms whereby long non-coding RNAs (lncRNAs) are associated with PTC pathogenesis.\nMETHODS: Microarray analysis was used to determine differentially expressed lncRNAs between paired PTC tissues and normal adjacent thyroid tissues. Quantitative RT-PCR was used for validation in 86 PTC cases. Small interfering RNA (siRNA) transfection assays were then performed to assess how a novel lncRNA affected key proliferation and cell death pathways in IHH4 PTC cells.\nRESULTS: We identified 1878 differentially expressed lncRNAs versus matched control samples (fold change \u22652.0, P\u2009<\u20090.05), of which 429 were upregulated and 1449 were downregulated. ENST00000539653.1 (ENS-653), one of the top hits in this microarray, was selected for further study. Higher ENS-653 expression was observed in PTC tissue samples versus adjacent normal tissues, and was associated with a larger tumor size and a more advanced clinical stage. In the Cancer Genome Atlas (TCGA) PTC cohort, higher ENS-653 expression was correlated with more frequent BRAF (V600E) mutation and poorer disease-free survival. Furthermore, ENS-653 downregulation reduced the proliferation of PTC cells and led to G1-S arrest, but had no impact on apoptosis. ENS-653 downregulation also inactivated ERK1/2 and ERK5, causing partial MAPK cascade suppression.\nCONCLUSION: ENS-653 exhibits oncogenic properties in PTC, and could be a diagnostic and/or prognostic PTC biomarker, in addition to possibly being a future target for therapy.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s12885-019-5533-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1024632", 
        "issn": [
          "1471-2407"
        ], 
        "name": "BMC Cancer", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "19"
      }
    ], 
    "name": "LncRNA ENST00000539653 acts as an oncogenic factor via MAPK signalling in papillary thyroid cancer", 
    "pagination": "297", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12885-019-5533-4"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "268adcd6e2fcec836712b69e1b1ab2b75065158afafac034faf260553ec59472"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1113179463"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100967800"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30940124"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12885-019-5533-4", 
      "https://app.dimensions.ai/details/publication/pub.1113179463"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-15T09:13", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000376_0000000376/records_56159_00000006.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs12885-019-5533-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12885-019-5533-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12885-019-5533-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12885-019-5533-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12885-019-5533-4'


 

This table displays all metadata directly associated to this object as RDF triples.

228 TRIPLES      21 PREDICATES      64 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12885-019-5533-4 schema:about anzsrc-for:11
2 anzsrc-for:1112
3 schema:author N2d66385d8f1647f485f1666025bed0ec
4 schema:citation sg:pub.10.1007/s12032-016-0816-y
5 sg:pub.10.1007/s13277-015-4149-9
6 sg:pub.10.1038/nrc3431
7 sg:pub.10.1038/s41598-017-01957-0
8 sg:pub.10.1186/s12943-017-0588-9
9 sg:pub.10.1186/s12943-017-0756-y
10 https://doi.org/10.1002/jcb.26142
11 https://doi.org/10.1016/j.biopha.2016.05.039
12 https://doi.org/10.1016/j.ccell.2016.03.010
13 https://doi.org/10.1016/j.cell.2014.09.050
14 https://doi.org/10.1016/s0140-6736(13)60109-9
15 https://doi.org/10.1056/nejmra1501993
16 https://doi.org/10.1080/15384101.2016.1261768
17 https://doi.org/10.1089/thy.2015.0020
18 https://doi.org/10.1089/thy.2015.0193
19 https://doi.org/10.1093/hmg/ddw056
20 https://doi.org/10.1111/febs.13741
21 https://doi.org/10.1136/bmj.g3045
22 https://doi.org/10.1158/0008-5472.can-13-3440
23 https://doi.org/10.1159/000438637
24 https://doi.org/10.1210/jc.2015-2247
25 https://doi.org/10.1210/jc.2016-1991
26 https://doi.org/10.1371/journal.pone.0176287
27 https://doi.org/10.1530/erc-17-0188
28 https://doi.org/10.1586/14737140.9.3.317
29 https://doi.org/10.1620/tjem.241.29
30 https://doi.org/10.18632/oncotarget.10825
31 https://doi.org/10.18632/oncotarget.11087
32 https://doi.org/10.18632/oncotarget.13725
33 https://doi.org/10.18632/oncotarget.15628
34 https://doi.org/10.18632/oncotarget.7828
35 https://doi.org/10.3322/caac.21254
36 https://doi.org/10.3322/caac.21338
37 https://doi.org/10.3892/ijo.2016.3803
38 https://doi.org/10.4143/crt.2015.118
39 schema:datePublished 2019-12
40 schema:datePublishedReg 2019-12-01
41 schema:description BACKGROUND: Papillary thyroid cancer (PTC) is the most frequent type of thyroid malignancy. In this study, we investigated the mechanisms whereby long non-coding RNAs (lncRNAs) are associated with PTC pathogenesis. METHODS: Microarray analysis was used to determine differentially expressed lncRNAs between paired PTC tissues and normal adjacent thyroid tissues. Quantitative RT-PCR was used for validation in 86 PTC cases. Small interfering RNA (siRNA) transfection assays were then performed to assess how a novel lncRNA affected key proliferation and cell death pathways in IHH4 PTC cells. RESULTS: We identified 1878 differentially expressed lncRNAs versus matched control samples (fold change ≥2.0, P < 0.05), of which 429 were upregulated and 1449 were downregulated. ENST00000539653.1 (ENS-653), one of the top hits in this microarray, was selected for further study. Higher ENS-653 expression was observed in PTC tissue samples versus adjacent normal tissues, and was associated with a larger tumor size and a more advanced clinical stage. In the Cancer Genome Atlas (TCGA) PTC cohort, higher ENS-653 expression was correlated with more frequent BRAF (V600E) mutation and poorer disease-free survival. Furthermore, ENS-653 downregulation reduced the proliferation of PTC cells and led to G1-S arrest, but had no impact on apoptosis. ENS-653 downregulation also inactivated ERK1/2 and ERK5, causing partial MAPK cascade suppression. CONCLUSION: ENS-653 exhibits oncogenic properties in PTC, and could be a diagnostic and/or prognostic PTC biomarker, in addition to possibly being a future target for therapy.
42 schema:genre research_article
43 schema:inLanguage en
44 schema:isAccessibleForFree true
45 schema:isPartOf N1eab5df43b254701b36daff5a1b4147e
46 N8deea363f88140b5ba3850227148f37d
47 sg:journal.1024632
48 schema:name LncRNA ENST00000539653 acts as an oncogenic factor via MAPK signalling in papillary thyroid cancer
49 schema:pagination 297
50 schema:productId N1a7ac8b2004f48fe8940a3b2da6893b4
51 N92705acb3ba0476fb202bac7436c01d3
52 N9a2d3ded8a804f4aa73ffccc47c3ac51
53 Nc2d7a89fb5de420cadf3650d276140cd
54 Nee0d95848cfe4640b3644d705d396286
55 schema:sameAs https://app.dimensions.ai/details/publication/pub.1113179463
56 https://doi.org/10.1186/s12885-019-5533-4
57 schema:sdDatePublished 2019-04-15T09:13
58 schema:sdLicense https://scigraph.springernature.com/explorer/license/
59 schema:sdPublisher Nfeb557483e2f4e06bc4c80c97e61639a
60 schema:url https://link.springer.com/10.1186%2Fs12885-019-5533-4
61 sgo:license sg:explorer/license/
62 sgo:sdDataset articles
63 rdf:type schema:ScholarlyArticle
64 N02f9b7de68d04544b8cc37e7ad190ce1 schema:affiliation https://www.grid.ac/institutes/grid.452511.6
65 schema:familyName Zuo
66 schema:givenName Zhihua
67 rdf:type schema:Person
68 N1a7ac8b2004f48fe8940a3b2da6893b4 schema:name nlm_unique_id
69 schema:value 100967800
70 rdf:type schema:PropertyValue
71 N1eab5df43b254701b36daff5a1b4147e schema:volumeNumber 19
72 rdf:type schema:PublicationVolume
73 N2904c20b7c2c42ca83f19f84401f366a rdf:first Ned7bed7f22a949a7965e3e96b8de4be6
74 rdf:rest Nb27d8d08e06f483695d965506a641d7e
75 N2d66385d8f1647f485f1666025bed0ec rdf:first Nc4c9680aa099499786e57eccf7475e90
76 rdf:rest Nd3ac1943991b45fe840a03eab680582a
77 N3c1a7b7e26c74584b6e4a89b22954618 schema:affiliation https://www.grid.ac/institutes/grid.452511.6
78 schema:familyName Liu
79 schema:givenName Ling
80 rdf:type schema:Person
81 N3da4f21b5c8f4f1e92feea26b25962d6 schema:affiliation https://www.grid.ac/institutes/grid.452511.6
82 schema:familyName Ding
83 schema:givenName Dafa
84 rdf:type schema:Person
85 N4ae613fff5994b76aad8418647e4dfba rdf:first N3da4f21b5c8f4f1e92feea26b25962d6
86 rdf:rest Na08c4e5f92d747da8f395f6c29459749
87 N538c2a04e2ed42eb9d8beb14cd011cba schema:affiliation https://www.grid.ac/institutes/grid.452511.6
88 schema:familyName Hou
89 schema:givenName Dawei
90 rdf:type schema:Person
91 N580004a71ee34d71ab1fdee911b099e6 rdf:first N538c2a04e2ed42eb9d8beb14cd011cba
92 rdf:rest rdf:nil
93 N6a87d81363be4c6dba02d253ca5888bd schema:affiliation https://www.grid.ac/institutes/grid.452511.6
94 schema:familyName Lu
95 schema:givenName Yibing
96 rdf:type schema:Person
97 N8deea363f88140b5ba3850227148f37d schema:issueNumber 1
98 rdf:type schema:PublicationIssue
99 N92705acb3ba0476fb202bac7436c01d3 schema:name readcube_id
100 schema:value 268adcd6e2fcec836712b69e1b1ab2b75065158afafac034faf260553ec59472
101 rdf:type schema:PropertyValue
102 N9a2d3ded8a804f4aa73ffccc47c3ac51 schema:name doi
103 schema:value 10.1186/s12885-019-5533-4
104 rdf:type schema:PropertyValue
105 Na08c4e5f92d747da8f395f6c29459749 rdf:first N6a87d81363be4c6dba02d253ca5888bd
106 rdf:rest N580004a71ee34d71ab1fdee911b099e6
107 Nb27d8d08e06f483695d965506a641d7e rdf:first N3c1a7b7e26c74584b6e4a89b22954618
108 rdf:rest N4ae613fff5994b76aad8418647e4dfba
109 Nc2d7a89fb5de420cadf3650d276140cd schema:name pubmed_id
110 schema:value 30940124
111 rdf:type schema:PropertyValue
112 Nc4c9680aa099499786e57eccf7475e90 schema:affiliation https://www.grid.ac/institutes/grid.268415.c
113 schema:familyName Song
114 schema:givenName Bin
115 rdf:type schema:Person
116 Nc5e741de455a4757a6a8ac68720d58a3 schema:affiliation https://www.grid.ac/institutes/grid.452511.6
117 schema:familyName Li
118 schema:givenName Rurun
119 rdf:type schema:Person
120 Nd3ac1943991b45fe840a03eab680582a rdf:first Nc5e741de455a4757a6a8ac68720d58a3
121 rdf:rest Nff92cce5905249e19bc8b1ecf30166e5
122 Ned7bed7f22a949a7965e3e96b8de4be6 schema:affiliation https://www.grid.ac/institutes/grid.89957.3a
123 schema:familyName Tan
124 schema:givenName Juan
125 rdf:type schema:Person
126 Nee0d95848cfe4640b3644d705d396286 schema:name dimensions_id
127 schema:value pub.1113179463
128 rdf:type schema:PropertyValue
129 Nfeb557483e2f4e06bc4c80c97e61639a schema:name Springer Nature - SN SciGraph project
130 rdf:type schema:Organization
131 Nff92cce5905249e19bc8b1ecf30166e5 rdf:first N02f9b7de68d04544b8cc37e7ad190ce1
132 rdf:rest N2904c20b7c2c42ca83f19f84401f366a
133 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
134 schema:name Medical and Health Sciences
135 rdf:type schema:DefinedTerm
136 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
137 schema:name Oncology and Carcinogenesis
138 rdf:type schema:DefinedTerm
139 sg:journal.1024632 schema:issn 1471-2407
140 schema:name BMC Cancer
141 rdf:type schema:Periodical
142 sg:pub.10.1007/s12032-016-0816-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1004992572
143 https://doi.org/10.1007/s12032-016-0816-y
144 rdf:type schema:CreativeWork
145 sg:pub.10.1007/s13277-015-4149-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013839346
146 https://doi.org/10.1007/s13277-015-4149-9
147 rdf:type schema:CreativeWork
148 sg:pub.10.1038/nrc3431 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009089021
149 https://doi.org/10.1038/nrc3431
150 rdf:type schema:CreativeWork
151 sg:pub.10.1038/s41598-017-01957-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085330966
152 https://doi.org/10.1038/s41598-017-01957-0
153 rdf:type schema:CreativeWork
154 sg:pub.10.1186/s12943-017-0588-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083844984
155 https://doi.org/10.1186/s12943-017-0588-9
156 rdf:type schema:CreativeWork
157 sg:pub.10.1186/s12943-017-0756-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1100381828
158 https://doi.org/10.1186/s12943-017-0756-y
159 rdf:type schema:CreativeWork
160 https://doi.org/10.1002/jcb.26142 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085432454
161 rdf:type schema:CreativeWork
162 https://doi.org/10.1016/j.biopha.2016.05.039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000785264
163 rdf:type schema:CreativeWork
164 https://doi.org/10.1016/j.ccell.2016.03.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017662736
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1016/j.cell.2014.09.050 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006907054
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1016/s0140-6736(13)60109-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030980548
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1056/nejmra1501993 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044889611
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1080/15384101.2016.1261768 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058402464
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1089/thy.2015.0020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007626247
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1089/thy.2015.0193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059322057
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1093/hmg/ddw056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059645840
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1111/febs.13741 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010308503
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1136/bmj.g3045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028650298
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1158/0008-5472.can-13-3440 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051068625
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1159/000438637 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012476927
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1210/jc.2015-2247 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064295867
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1210/jc.2016-1991 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000606849
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1371/journal.pone.0176287 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085021725
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1530/erc-17-0188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092737717
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1586/14737140.9.3.317 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013484119
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1620/tjem.241.29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050757498
199 rdf:type schema:CreativeWork
200 https://doi.org/10.18632/oncotarget.10825 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018898154
201 rdf:type schema:CreativeWork
202 https://doi.org/10.18632/oncotarget.11087 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027736775
203 rdf:type schema:CreativeWork
204 https://doi.org/10.18632/oncotarget.13725 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021943990
205 rdf:type schema:CreativeWork
206 https://doi.org/10.18632/oncotarget.15628 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084372776
207 rdf:type schema:CreativeWork
208 https://doi.org/10.18632/oncotarget.7828 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053537886
209 rdf:type schema:CreativeWork
210 https://doi.org/10.3322/caac.21254 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011682275
211 rdf:type schema:CreativeWork
212 https://doi.org/10.3322/caac.21338 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010436755
213 rdf:type schema:CreativeWork
214 https://doi.org/10.3892/ijo.2016.3803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071514638
215 rdf:type schema:CreativeWork
216 https://doi.org/10.4143/crt.2015.118 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072255994
217 rdf:type schema:CreativeWork
218 https://www.grid.ac/institutes/grid.268415.c schema:alternateName Yangzhou University
219 schema:name Department of Endocrinology, Clinical Medical College, Yangzhou University, 98 Nantong West Road, 225001, Yangzhou, China
220 Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, 210011, Nanjing, China
221 rdf:type schema:Organization
222 https://www.grid.ac/institutes/grid.452511.6 schema:alternateName Second Affiliated Hospital of Nanjing Medical University
223 schema:name Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, 210011, Nanjing, China
224 Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, 210011, Nanjing, China
225 rdf:type schema:Organization
226 https://www.grid.ac/institutes/grid.89957.3a schema:alternateName Nanjing Medical University
227 schema:name Department of Gerontology, Huai’an First People’s Hospital, Nanjing Medical University, 6 Beijing West Road, 223300, Huai’an, China
228 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...