The two isoforms of matrix metalloproteinase- 2 have distinct renal spatial and temporal distributions in murine models of types 1 ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-09-25

AUTHORS

Il Young Kim, Sang Soo Kim, Hye Won Lee, Sun Sik Bae, Hong Koo Ha, Eun Soon Jung, Min Young Lee, Miyeun Han, Harin Rhee, Eun Young Seong, Dong Won Lee, Soo Bong Lee, David H. Lovett, Sang Heon Song

ABSTRACT

BackgroundWe recently reported on the enhanced tubular expression of two discrete isoforms of the MMP-2 (full length and N-terminal truncated, FL-MMP-2, NTT-MMP-2) in a murine model and human diabetic kidneys. In the present study, we examined in more detail the temporal and spatial distributions of MMP-2 isoform expression in murine models of Type 1 and Type 2 diabetes mellitus.MethodsDiabetic models were streptozotocin (STZ)-induced diabetes (Type 1 diabetes mellitus) and db/db mice (Type 2 diabetes mellitus). We quantified the abundance of two isoforms of MMP-2 transcripts by qPCR. A spatial distribution of two isoforms of MMP-2 was analyzed semi-quantitatively according to time after injection of STZ and with increasing age of db/db mice. Furthermore, immunohistochemistry for nitrotyrosine was performed to examine a potential association between oxidative stress and MMP-2 isoform expression.ResultsBoth isoforms of MMP-2 were upregulated in whole kidneys from STZ and db/db mice. In the case of FL-MMP-2, mRNA levels significantly increased at 12 and 24 weeks in STZ mice, while the isoform expression was significantly increased only at 16 weeks, in the db/db mice. FL-MMP-2 protein levels increased in the cortices and outer medullae of both STZ and db/db mice as a function of the duration of diabetes. For NTT-MMP-2, mRNA levels increased earlier at 4 weeks in STZ mice and at 10 weeks of age in db/db mice. The expression of NTT-MMP-2 also increased, primarily in the cortices of STZ and db/db mice, as a function of the duration of diabetes. Quantitatively, these findings were consistent with the qPCR results in the case of NTT-MMP-2, respectively (STZ 24 weeks, 3.24 ± 3.70 fold; 16 weeks db/db, 4.49 ± 0.55 fold). In addition, nitrotyrosine was expressed primarily in cortex as compared to medulla as a function of the duration of diabetes similar to NTT-MMP-2 expression.ConclusionsTwo isoforms of MMP-2 are highly inducible in two diabetic murine models and become more abundant as a function of time. As the expression patterns were not the same in the two isoforms of MMP-2, it is possible that each isoform has a discrete role in the development of diabetic renal injury. More... »

PAGES

248

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12882-018-1029-8

DOI

http://dx.doi.org/10.1186/s12882-018-1029-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1107228032

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30253743


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Diabetes Mellitus, Experimental", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Diabetes Mellitus, Type 1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Diabetes Mellitus, Type 2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Isoenzymes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kidney", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kidney Cortex", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kidney Medulla", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kidney Tubules", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Matrix Metalloproteinase 2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred C57BL", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oxidative Stress", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Messenger", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reverse Transcriptase Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tyrosine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Up-Regulation", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Research Institute for Convergence of Biomedical Science and Technology and Department of Internal Medicine, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnamdo, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.412591.a", 
          "name": [
            "Research Institute for Convergence of Biomedical Science and Technology and Department of Internal Medicine, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnamdo, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Il Young", 
        "id": "sg:person.0664337220.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664337220.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.412588.2", 
          "name": [
            "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Sang Soo", 
        "id": "sg:person.01122642102.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01122642102.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.412588.2", 
          "name": [
            "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Hye Won", 
        "id": "sg:person.01145151552.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145151552.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "MRC for Ischemic Tissue Regeneration, Medical Research Institute, and Department of Pharmacology, Pusan National University School of Medicine, Yangsan, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.262229.f", 
          "name": [
            "MRC for Ischemic Tissue Regeneration, Medical Research Institute, and Department of Pharmacology, Pusan National University School of Medicine, Yangsan, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bae", 
        "givenName": "Sun Sik", 
        "id": "sg:person.01113214531.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113214531.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biomedical Research Institute and Department of Urology, Pusan National University Hospital, Busan, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.412588.2", 
          "name": [
            "Biomedical Research Institute and Department of Urology, Pusan National University Hospital, Busan, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ha", 
        "givenName": "Hong Koo", 
        "id": "sg:person.0712676025.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0712676025.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.412588.2", 
          "name": [
            "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jung", 
        "givenName": "Eun Soon", 
        "id": "sg:person.07413214560.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07413214560.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.412588.2", 
          "name": [
            "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Min Young", 
        "id": "sg:person.01013066364.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01013066364.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.412588.2", 
          "name": [
            "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Han", 
        "givenName": "Miyeun", 
        "id": "sg:person.01144036024.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144036024.89"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.412588.2", 
          "name": [
            "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rhee", 
        "givenName": "Harin", 
        "id": "sg:person.01215107345.87", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215107345.87"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.412588.2", 
          "name": [
            "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Seong", 
        "givenName": "Eun Young", 
        "id": "sg:person.01124673362.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01124673362.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Research Institute for Convergence of Biomedical Science and Technology and Department of Internal Medicine, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnamdo, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.412591.a", 
          "name": [
            "Research Institute for Convergence of Biomedical Science and Technology and Department of Internal Medicine, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnamdo, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Dong Won", 
        "id": "sg:person.01307731474.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307731474.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Research Institute for Convergence of Biomedical Science and Technology and Department of Internal Medicine, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnamdo, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.412591.a", 
          "name": [
            "Research Institute for Convergence of Biomedical Science and Technology and Department of Internal Medicine, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnamdo, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Soo Bong", 
        "id": "sg:person.01147015665.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147015665.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Department of Medicine, San Francisco Department of Veterans Affairs Medical Center, University of California San Francisco, California, USA", 
          "id": "http://www.grid.ac/institutes/grid.266102.1", 
          "name": [
            "The Department of Medicine, San Francisco Department of Veterans Affairs Medical Center, University of California San Francisco, California, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lovett", 
        "givenName": "David H.", 
        "id": "sg:person.01036274500.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036274500.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea", 
          "id": "http://www.grid.ac/institutes/grid.412588.2", 
          "name": [
            "Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Song", 
        "givenName": "Sang Heon", 
        "id": "sg:person.01032567265.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01032567265.78"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2018-09-25", 
    "datePublishedReg": "2018-09-25", 
    "description": "BackgroundWe recently reported on the enhanced tubular expression of two discrete isoforms of the MMP-2 (full length and N-terminal truncated, FL-MMP-2, NTT-MMP-2) in a murine model and human diabetic kidneys. In the present study, we examined in more detail the temporal and spatial distributions of MMP-2 isoform expression in murine models of Type 1 and Type 2 diabetes mellitus.MethodsDiabetic models were streptozotocin (STZ)-induced diabetes (Type 1 diabetes mellitus) and db/db mice (Type 2 diabetes mellitus). We quantified the abundance of two isoforms of MMP-2 transcripts by qPCR. A spatial distribution of two isoforms of MMP-2 was analyzed semi-quantitatively according to time after injection of STZ and with increasing age of db/db mice. Furthermore, immunohistochemistry for nitrotyrosine was performed to examine a potential association between oxidative stress and MMP-2 isoform expression.ResultsBoth isoforms of MMP-2 were upregulated in whole kidneys from STZ and db/db mice. In the case of FL-MMP-2, mRNA levels significantly increased at 12 and 24\u00a0weeks in STZ mice, while the isoform expression was significantly increased only at 16\u00a0weeks, in the db/db mice. FL-MMP-2 protein levels increased in the cortices and outer medullae of both STZ and db/db mice as a function of the duration of diabetes. For NTT-MMP-2, mRNA levels increased earlier at 4\u00a0weeks in STZ mice and at 10\u00a0weeks of age in db/db mice. The expression of NTT-MMP-2 also increased, primarily in the cortices of STZ and db/db mice, as a function of the duration of diabetes. Quantitatively, these findings were consistent with the qPCR results in the case of NTT-MMP-2, respectively (STZ 24\u00a0weeks, 3.24\u2009\u00b1\u20093.70 fold; 16\u00a0weeks db/db, 4.49\u2009\u00b1\u20090.55 fold). In addition, nitrotyrosine was expressed primarily in cortex as compared to medulla as a function of the duration of diabetes similar to NTT-MMP-2 expression.ConclusionsTwo isoforms of MMP-2 are highly inducible in two diabetic murine models and become more abundant as a function of time. As the expression patterns were not the same in the two isoforms of MMP-2, it is possible that each isoform has a discrete role in the development of diabetic renal injury.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/s12882-018-1029-8", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2493830", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2401364", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1024626", 
        "issn": [
          "1471-2369"
        ], 
        "name": "BMC Nephrology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "19"
      }
    ], 
    "keywords": [
      "db/db mice", 
      "NTT-MMP-2", 
      "duration of diabetes", 
      "db mice", 
      "murine model", 
      "MMP-2", 
      "STZ mice", 
      "diabetes mellitus", 
      "NTT-MMP-2 expression", 
      "type 1", 
      "type 2 diabetes mellitus", 
      "isoform expression", 
      "FL-MMP-2", 
      "diabetic renal injury", 
      "injection of STZ", 
      "mRNA levels", 
      "diabetic murine model", 
      "human diabetic kidneys", 
      "matrix metalloproteinase-2", 
      "weeks of age", 
      "MMP-2 transcripts", 
      "diabetic kidney", 
      "renal injury", 
      "tubular expression", 
      "metalloproteinase-2", 
      "STZ", 
      "whole kidney", 
      "diabetes", 
      "potential association", 
      "outer medulla", 
      "mice", 
      "oxidative stress", 
      "weeks", 
      "protein levels", 
      "cortex", 
      "mellitus", 
      "nitrotyrosine", 
      "medulla", 
      "kidney", 
      "present study", 
      "duration", 
      "age", 
      "isoforms", 
      "expression", 
      "qPCR results", 
      "discrete isoforms", 
      "expression patterns", 
      "streptozotocin", 
      "discrete roles", 
      "BackgroundWe", 
      "immunohistochemistry", 
      "injury", 
      "levels", 
      "injection", 
      "cases", 
      "association", 
      "qPCR", 
      "function", 
      "findings", 
      "study", 
      "role", 
      "time", 
      "transcripts", 
      "patterns", 
      "stress", 
      "addition", 
      "development", 
      "model", 
      "more detail", 
      "results", 
      "distribution", 
      "function of time", 
      "temporal distribution", 
      "abundance", 
      "detail", 
      "spatial distribution"
    ], 
    "name": "The two isoforms of matrix metalloproteinase- 2 have distinct renal spatial and temporal distributions in murine models of types 1 and 2 diabetes mellitus", 
    "pagination": "248", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1107228032"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12882-018-1029-8"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30253743"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12882-018-1029-8", 
      "https://app.dimensions.ai/details/publication/pub.1107228032"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-06-01T22:17", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_784.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/s12882-018-1029-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12882-018-1029-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12882-018-1029-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12882-018-1029-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12882-018-1029-8'


 

This table displays all metadata directly associated to this object as RDF triples.

319 TRIPLES      21 PREDICATES      121 URIs      113 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12882-018-1029-8 schema:about N05747186d0aa4873870fb9cf3c587f43
2 N099d11a06f9a49f4aa568c0d0562a0cd
3 N132aa60e959444018e8bdc466cd691c9
4 N1947a66ca95843c59c6083bf6746d3b5
5 N39dff05e7f9b4948ac836375cb4bbd4b
6 N4bfac9d41ecf463689a989e38a2c6fb0
7 N52c8b0424e934b0d8b74ed3bed35a250
8 N5d137377fed948afb029b8f182cce087
9 N5e7b2d93f9604fd5aaa0d594b85ca15d
10 N5ec107c3cb674c88b4f735d3427ae37e
11 N8529e439df904aeda524b42804065a44
12 N936fbb3de051439396ed910ec277a9f5
13 Na5c460e6696b429293312f1e1cc6775e
14 Na8b1b0a5ea2346e08a15fc52e12aaade
15 Nacdbc24bb9cc453ba1d84749724248bc
16 Nb07e32bd403c40f9ae30b1aa1a40b871
17 Nb34141b25bda4f75b2c91cc840930c18
18 Ne054e80cbcc9498c8648a5f78a36adbd
19 Nfbd1d2c0ae984138880e62d0b5d2d761
20 anzsrc-for:11
21 anzsrc-for:1103
22 schema:author N79b3bd1cfd844b8fbc205c7c95196c45
23 schema:datePublished 2018-09-25
24 schema:datePublishedReg 2018-09-25
25 schema:description BackgroundWe recently reported on the enhanced tubular expression of two discrete isoforms of the MMP-2 (full length and N-terminal truncated, FL-MMP-2, NTT-MMP-2) in a murine model and human diabetic kidneys. In the present study, we examined in more detail the temporal and spatial distributions of MMP-2 isoform expression in murine models of Type 1 and Type 2 diabetes mellitus.MethodsDiabetic models were streptozotocin (STZ)-induced diabetes (Type 1 diabetes mellitus) and db/db mice (Type 2 diabetes mellitus). We quantified the abundance of two isoforms of MMP-2 transcripts by qPCR. A spatial distribution of two isoforms of MMP-2 was analyzed semi-quantitatively according to time after injection of STZ and with increasing age of db/db mice. Furthermore, immunohistochemistry for nitrotyrosine was performed to examine a potential association between oxidative stress and MMP-2 isoform expression.ResultsBoth isoforms of MMP-2 were upregulated in whole kidneys from STZ and db/db mice. In the case of FL-MMP-2, mRNA levels significantly increased at 12 and 24 weeks in STZ mice, while the isoform expression was significantly increased only at 16 weeks, in the db/db mice. FL-MMP-2 protein levels increased in the cortices and outer medullae of both STZ and db/db mice as a function of the duration of diabetes. For NTT-MMP-2, mRNA levels increased earlier at 4 weeks in STZ mice and at 10 weeks of age in db/db mice. The expression of NTT-MMP-2 also increased, primarily in the cortices of STZ and db/db mice, as a function of the duration of diabetes. Quantitatively, these findings were consistent with the qPCR results in the case of NTT-MMP-2, respectively (STZ 24 weeks, 3.24 ± 3.70 fold; 16 weeks db/db, 4.49 ± 0.55 fold). In addition, nitrotyrosine was expressed primarily in cortex as compared to medulla as a function of the duration of diabetes similar to NTT-MMP-2 expression.ConclusionsTwo isoforms of MMP-2 are highly inducible in two diabetic murine models and become more abundant as a function of time. As the expression patterns were not the same in the two isoforms of MMP-2, it is possible that each isoform has a discrete role in the development of diabetic renal injury.
26 schema:genre article
27 schema:inLanguage en
28 schema:isAccessibleForFree true
29 schema:isPartOf N672e4c92789e4150a129198e8f474e50
30 N79a7c18b76f24bd899a99a63598f0559
31 sg:journal.1024626
32 schema:keywords BackgroundWe
33 FL-MMP-2
34 MMP-2
35 MMP-2 transcripts
36 NTT-MMP-2
37 NTT-MMP-2 expression
38 STZ
39 STZ mice
40 abundance
41 addition
42 age
43 association
44 cases
45 cortex
46 db mice
47 db/db mice
48 detail
49 development
50 diabetes
51 diabetes mellitus
52 diabetic kidney
53 diabetic murine model
54 diabetic renal injury
55 discrete isoforms
56 discrete roles
57 distribution
58 duration
59 duration of diabetes
60 expression
61 expression patterns
62 findings
63 function
64 function of time
65 human diabetic kidneys
66 immunohistochemistry
67 injection
68 injection of STZ
69 injury
70 isoform expression
71 isoforms
72 kidney
73 levels
74 mRNA levels
75 matrix metalloproteinase-2
76 medulla
77 mellitus
78 metalloproteinase-2
79 mice
80 model
81 more detail
82 murine model
83 nitrotyrosine
84 outer medulla
85 oxidative stress
86 patterns
87 potential association
88 present study
89 protein levels
90 qPCR
91 qPCR results
92 renal injury
93 results
94 role
95 spatial distribution
96 streptozotocin
97 stress
98 study
99 temporal distribution
100 time
101 transcripts
102 tubular expression
103 type 1
104 type 2 diabetes mellitus
105 weeks
106 weeks of age
107 whole kidney
108 schema:name The two isoforms of matrix metalloproteinase- 2 have distinct renal spatial and temporal distributions in murine models of types 1 and 2 diabetes mellitus
109 schema:pagination 248
110 schema:productId Na3998aff5dd248b58d47349f5ed9f841
111 Nae78850902a748ac890927d57e4150a2
112 Nd0abbcf42bc740b9aad9c940d6754a4b
113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107228032
114 https://doi.org/10.1186/s12882-018-1029-8
115 schema:sdDatePublished 2022-06-01T22:17
116 schema:sdLicense https://scigraph.springernature.com/explorer/license/
117 schema:sdPublisher N39b30c8f0925403ebd816658ea5bc3c1
118 schema:url https://doi.org/10.1186/s12882-018-1029-8
119 sgo:license sg:explorer/license/
120 sgo:sdDataset articles
121 rdf:type schema:ScholarlyArticle
122 N05747186d0aa4873870fb9cf3c587f43 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Oxidative Stress
124 rdf:type schema:DefinedTerm
125 N099d11a06f9a49f4aa568c0d0562a0cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Kidney Medulla
127 rdf:type schema:DefinedTerm
128 N0ab45a041b024dc9ad1dbd4037392cbb rdf:first sg:person.01215107345.87
129 rdf:rest Nd9d4eeecf730487393998405255f60d3
130 N132aa60e959444018e8bdc466cd691c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Male
132 rdf:type schema:DefinedTerm
133 N1947a66ca95843c59c6083bf6746d3b5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Diabetes Mellitus, Type 1
135 rdf:type schema:DefinedTerm
136 N2c6b2a2ed1424ea2ad2d6926fc87f3cc rdf:first sg:person.01032567265.78
137 rdf:rest rdf:nil
138 N39b30c8f0925403ebd816658ea5bc3c1 schema:name Springer Nature - SN SciGraph project
139 rdf:type schema:Organization
140 N39dff05e7f9b4948ac836375cb4bbd4b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Animals
142 rdf:type schema:DefinedTerm
143 N406bdadd1ce34328af24dae8f6f6210f rdf:first sg:person.07413214560.42
144 rdf:rest N7437d07117e74201b4ffa1603fff2bdf
145 N4bfac9d41ecf463689a989e38a2c6fb0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Tyrosine
147 rdf:type schema:DefinedTerm
148 N52c8b0424e934b0d8b74ed3bed35a250 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Diabetes Mellitus, Experimental
150 rdf:type schema:DefinedTerm
151 N5d137377fed948afb029b8f182cce087 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Matrix Metalloproteinase 2
153 rdf:type schema:DefinedTerm
154 N5e7b2d93f9604fd5aaa0d594b85ca15d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Mice, Inbred C57BL
156 rdf:type schema:DefinedTerm
157 N5ec107c3cb674c88b4f735d3427ae37e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Up-Regulation
159 rdf:type schema:DefinedTerm
160 N672e4c92789e4150a129198e8f474e50 schema:volumeNumber 19
161 rdf:type schema:PublicationVolume
162 N7437d07117e74201b4ffa1603fff2bdf rdf:first sg:person.01013066364.02
163 rdf:rest Nbd6b9142542b403593dd446d00daaf17
164 N79a7c18b76f24bd899a99a63598f0559 schema:issueNumber 1
165 rdf:type schema:PublicationIssue
166 N79b3bd1cfd844b8fbc205c7c95196c45 rdf:first sg:person.0664337220.16
167 rdf:rest Nf0dbb71c8c324677b1ca6a3fb97d260b
168 N7bb9c8f251ee4ef38d92f2bc52b53109 rdf:first sg:person.01113214531.31
169 rdf:rest Na1d07dbf98304b7296b19e59da6f19dc
170 N8529e439df904aeda524b42804065a44 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Reverse Transcriptase Polymerase Chain Reaction
172 rdf:type schema:DefinedTerm
173 N86c02c297f7046f08c4960d7ca1abe95 rdf:first sg:person.01147015665.41
174 rdf:rest N9cf5227dfaf84f27b9b5557fed5b0034
175 N936fbb3de051439396ed910ec277a9f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Diabetes Mellitus, Type 2
177 rdf:type schema:DefinedTerm
178 N9cf5227dfaf84f27b9b5557fed5b0034 rdf:first sg:person.01036274500.13
179 rdf:rest N2c6b2a2ed1424ea2ad2d6926fc87f3cc
180 Na1d07dbf98304b7296b19e59da6f19dc rdf:first sg:person.0712676025.34
181 rdf:rest N406bdadd1ce34328af24dae8f6f6210f
182 Na3998aff5dd248b58d47349f5ed9f841 schema:name pubmed_id
183 schema:value 30253743
184 rdf:type schema:PropertyValue
185 Na5c460e6696b429293312f1e1cc6775e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
186 schema:name Kidney Tubules
187 rdf:type schema:DefinedTerm
188 Na8b1b0a5ea2346e08a15fc52e12aaade schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Isoenzymes
190 rdf:type schema:DefinedTerm
191 Nacdbc24bb9cc453ba1d84749724248bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Mice
193 rdf:type schema:DefinedTerm
194 Nae78850902a748ac890927d57e4150a2 schema:name doi
195 schema:value 10.1186/s12882-018-1029-8
196 rdf:type schema:PropertyValue
197 Nb07e32bd403c40f9ae30b1aa1a40b871 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
198 schema:name Kidney
199 rdf:type schema:DefinedTerm
200 Nb34141b25bda4f75b2c91cc840930c18 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
201 schema:name Disease Models, Animal
202 rdf:type schema:DefinedTerm
203 Nbd6b9142542b403593dd446d00daaf17 rdf:first sg:person.01144036024.89
204 rdf:rest N0ab45a041b024dc9ad1dbd4037392cbb
205 Nd0abbcf42bc740b9aad9c940d6754a4b schema:name dimensions_id
206 schema:value pub.1107228032
207 rdf:type schema:PropertyValue
208 Nd9d4eeecf730487393998405255f60d3 rdf:first sg:person.01124673362.08
209 rdf:rest Nec5dff274aef4b9080dc2c0f9f562410
210 Ne054e80cbcc9498c8648a5f78a36adbd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
211 schema:name RNA, Messenger
212 rdf:type schema:DefinedTerm
213 Ne72b7398c52e41768e8c0047f8baefd5 rdf:first sg:person.01145151552.05
214 rdf:rest N7bb9c8f251ee4ef38d92f2bc52b53109
215 Nec5dff274aef4b9080dc2c0f9f562410 rdf:first sg:person.01307731474.44
216 rdf:rest N86c02c297f7046f08c4960d7ca1abe95
217 Nf0dbb71c8c324677b1ca6a3fb97d260b rdf:first sg:person.01122642102.04
218 rdf:rest Ne72b7398c52e41768e8c0047f8baefd5
219 Nfbd1d2c0ae984138880e62d0b5d2d761 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
220 schema:name Kidney Cortex
221 rdf:type schema:DefinedTerm
222 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
223 schema:name Medical and Health Sciences
224 rdf:type schema:DefinedTerm
225 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
226 schema:name Clinical Sciences
227 rdf:type schema:DefinedTerm
228 sg:grant.2401364 http://pending.schema.org/fundedItem sg:pub.10.1186/s12882-018-1029-8
229 rdf:type schema:MonetaryGrant
230 sg:grant.2493830 http://pending.schema.org/fundedItem sg:pub.10.1186/s12882-018-1029-8
231 rdf:type schema:MonetaryGrant
232 sg:journal.1024626 schema:issn 1471-2369
233 schema:name BMC Nephrology
234 schema:publisher Springer Nature
235 rdf:type schema:Periodical
236 sg:person.01013066364.02 schema:affiliation grid-institutes:grid.412588.2
237 schema:familyName Lee
238 schema:givenName Min Young
239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01013066364.02
240 rdf:type schema:Person
241 sg:person.01032567265.78 schema:affiliation grid-institutes:grid.412588.2
242 schema:familyName Song
243 schema:givenName Sang Heon
244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01032567265.78
245 rdf:type schema:Person
246 sg:person.01036274500.13 schema:affiliation grid-institutes:grid.266102.1
247 schema:familyName Lovett
248 schema:givenName David H.
249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036274500.13
250 rdf:type schema:Person
251 sg:person.01113214531.31 schema:affiliation grid-institutes:grid.262229.f
252 schema:familyName Bae
253 schema:givenName Sun Sik
254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113214531.31
255 rdf:type schema:Person
256 sg:person.01122642102.04 schema:affiliation grid-institutes:grid.412588.2
257 schema:familyName Kim
258 schema:givenName Sang Soo
259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01122642102.04
260 rdf:type schema:Person
261 sg:person.01124673362.08 schema:affiliation grid-institutes:grid.412588.2
262 schema:familyName Seong
263 schema:givenName Eun Young
264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01124673362.08
265 rdf:type schema:Person
266 sg:person.01144036024.89 schema:affiliation grid-institutes:grid.412588.2
267 schema:familyName Han
268 schema:givenName Miyeun
269 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144036024.89
270 rdf:type schema:Person
271 sg:person.01145151552.05 schema:affiliation grid-institutes:grid.412588.2
272 schema:familyName Lee
273 schema:givenName Hye Won
274 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01145151552.05
275 rdf:type schema:Person
276 sg:person.01147015665.41 schema:affiliation grid-institutes:grid.412591.a
277 schema:familyName Lee
278 schema:givenName Soo Bong
279 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147015665.41
280 rdf:type schema:Person
281 sg:person.01215107345.87 schema:affiliation grid-institutes:grid.412588.2
282 schema:familyName Rhee
283 schema:givenName Harin
284 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215107345.87
285 rdf:type schema:Person
286 sg:person.01307731474.44 schema:affiliation grid-institutes:grid.412591.a
287 schema:familyName Lee
288 schema:givenName Dong Won
289 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307731474.44
290 rdf:type schema:Person
291 sg:person.0664337220.16 schema:affiliation grid-institutes:grid.412591.a
292 schema:familyName Kim
293 schema:givenName Il Young
294 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664337220.16
295 rdf:type schema:Person
296 sg:person.0712676025.34 schema:affiliation grid-institutes:grid.412588.2
297 schema:familyName Ha
298 schema:givenName Hong Koo
299 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0712676025.34
300 rdf:type schema:Person
301 sg:person.07413214560.42 schema:affiliation grid-institutes:grid.412588.2
302 schema:familyName Jung
303 schema:givenName Eun Soon
304 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07413214560.42
305 rdf:type schema:Person
306 grid-institutes:grid.262229.f schema:alternateName MRC for Ischemic Tissue Regeneration, Medical Research Institute, and Department of Pharmacology, Pusan National University School of Medicine, Yangsan, Republic of Korea
307 schema:name MRC for Ischemic Tissue Regeneration, Medical Research Institute, and Department of Pharmacology, Pusan National University School of Medicine, Yangsan, Republic of Korea
308 rdf:type schema:Organization
309 grid-institutes:grid.266102.1 schema:alternateName The Department of Medicine, San Francisco Department of Veterans Affairs Medical Center, University of California San Francisco, California, USA
310 schema:name The Department of Medicine, San Francisco Department of Veterans Affairs Medical Center, University of California San Francisco, California, USA
311 rdf:type schema:Organization
312 grid-institutes:grid.412588.2 schema:alternateName Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea
313 Biomedical Research Institute and Department of Urology, Pusan National University Hospital, Busan, Republic of Korea
314 schema:name Biomedical Research Institute and Department of Internal Medicine, Pusan National University Hospital, Gudeok-ro 179 Seo-gu, 49241, Busan, Republic of Korea
315 Biomedical Research Institute and Department of Urology, Pusan National University Hospital, Busan, Republic of Korea
316 rdf:type schema:Organization
317 grid-institutes:grid.412591.a schema:alternateName Research Institute for Convergence of Biomedical Science and Technology and Department of Internal Medicine, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnamdo, Republic of Korea
318 schema:name Research Institute for Convergence of Biomedical Science and Technology and Department of Internal Medicine, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnamdo, Republic of Korea
319 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...