Ontology type: schema:ScholarlyArticle Open Access: True
2019-12
AUTHORSEva Dopico, Rodrigo Pimenta Del-Rei, Bertha Espinoza, Itziar Ubillos, Nilson Ivo Tonin Zanchin, Elena Sulleiro, Zaira Moure, Paola Alejandra Fiorani Celedon, Wayner Vieira Souza, Edimilson Domingos da Silva, Yara Miranda Gomes, Fred Luciano Neves Santos
ABSTRACTBACKGROUND: Chronic Chagas Disease (CD) diagnosis is based on serological methods employing crude, semipurified or recombinant antigens, which may result in low sensitivity or cross-reactivity. To reduce these restrictions, we developed a strategy involving use of molecules containing repetitive fragments of Trypanosoma cruzi conserved proteins. Diagnostic performance of IBMP-8.1 and IBMP-8.4 chimeric antigens (Molecular Biology Institute of Paraná - IBMP in Portuguese acronym) was assessed to diagnose T. cruzi-infected and non-infected immigrants living in Barcelona (Spain), a non-endemic setting for Chagas disease. METHODS: Reactivity of IBMP-8.1 and IBMP-8.4 was assessed using an in-house automated ELISA with 347 positive and 331 negative individuals to Chagas disease. Antigenic cross-reactivity was measured with sera samples from pregnant women with Toxoplasma gondii (n = 98) and Zika virus (n = 75) antibodies. RESULTS: The area under the curve values was 1 and 0.99 for the IBMP-8.1 and IBMP-8.4 proteins, respectively, demonstrating excellent diagnostic accuracy. The reactivity index was higher for IBMP-8.1 than IBMP-8.4 in positive samples and no significant difference in reactivity index was observed in negative samples. Sensitivity ranged from 99.4% for IBMP-8.1 to 99.1% for IBMP-8.4 and was not statistically different. Specificity for IBMP-8.1 reached 100 and 99.7% for IBMP-8.4, both nearly 100% accurate. No antigenic cross-reactivity was observed and reactivity index was similar to that for negative Chagas disease individuals. CONCLUSIONS: Our results showed an outstanding performance of IBMP-8.1 and IBMP-8.4 chimeric antigens by ELISA and suggest both chimeric antigens could also be used for Chagas disease diagnosis in immigrants living in non-endemic settings. More... »
PAGES251
http://scigraph.springernature.com/pub.10.1186/s12879-019-3872-z
DOIhttp://dx.doi.org/10.1186/s12879-019-3872-z
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1112765840
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/30871504
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical Microbiology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Institut Catal\u00e0 de la Salut",
"id": "https://www.grid.ac/institutes/grid.22061.37",
"name": [
"Laboratori Cl\u00ednic Territorial Metropolitana Sud, Catalan Institute of Health, Barcelona, Catalonia, Spain"
],
"type": "Organization"
},
"familyName": "Dopico",
"givenName": "Eva",
"type": "Person"
},
{
"affiliation": {
"name": [
"Faculty of Technology and Sciences of Bahia, Salvador, Bahia, Brazil"
],
"type": "Organization"
},
"familyName": "Del-Rei",
"givenName": "Rodrigo Pimenta",
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Autonomous University of Mexico",
"id": "https://www.grid.ac/institutes/grid.9486.3",
"name": [
"Instituto de Investigaciones Biom\u00e9dicas, Departamento de Inmunolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, Mexico"
],
"type": "Organization"
},
"familyName": "Espinoza",
"givenName": "Bertha",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institut Catal\u00e0 de la Salut",
"id": "https://www.grid.ac/institutes/grid.22061.37",
"name": [
"Laboratori Cl\u00ednic Territorial Metropolitana Sud, Catalan Institute of Health, Barcelona, Catalonia, Spain"
],
"type": "Organization"
},
"familyName": "Ubillos",
"givenName": "Itziar",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Oswaldo Cruz Foundation",
"id": "https://www.grid.ac/institutes/grid.418068.3",
"name": [
"Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Paran\u00e1, Brazil"
],
"type": "Organization"
},
"familyName": "Zanchin",
"givenName": "Nilson Ivo Tonin",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Hospital Universitari Vall d'Hebron",
"id": "https://www.grid.ac/institutes/grid.411083.f",
"name": [
"Microbiology Department, Vall d\u2019Hebron University Hospital, PROSICS, Barcelona, Catalonia, Spain"
],
"type": "Organization"
},
"familyName": "Sulleiro",
"givenName": "Elena",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Hospital Universitari Vall d'Hebron",
"id": "https://www.grid.ac/institutes/grid.411083.f",
"name": [
"Microbiology Department, Vall d\u2019Hebron University Hospital, PROSICS, Barcelona, Catalonia, Spain"
],
"type": "Organization"
},
"familyName": "Moure",
"givenName": "Zaira",
"type": "Person"
},
{
"affiliation": {
"name": [
"Molecular Biology Institute of Paran\u00e1, Curitiba, Paran\u00e1, Brazil"
],
"type": "Organization"
},
"familyName": "Celedon",
"givenName": "Paola Alejandra Fiorani",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Oswaldo Cruz Foundation",
"id": "https://www.grid.ac/institutes/grid.418068.3",
"name": [
"Aggeu Magalh\u00e3es Institute, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil"
],
"type": "Organization"
},
"familyName": "Souza",
"givenName": "Wayner Vieira",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Oswaldo Cruz Foundation",
"id": "https://www.grid.ac/institutes/grid.418068.3",
"name": [
"Immunobiological Technology Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil"
],
"type": "Organization"
},
"familyName": "da Silva",
"givenName": "Edimilson Domingos",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Oswaldo Cruz Foundation",
"id": "https://www.grid.ac/institutes/grid.418068.3",
"name": [
"Aggeu Magalh\u00e3es Institute, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil"
],
"type": "Organization"
},
"familyName": "Gomes",
"givenName": "Yara Miranda",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Oswaldo Cruz Foundation",
"id": "https://www.grid.ac/institutes/grid.418068.3",
"name": [
"Gon\u00e7alo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil"
],
"type": "Organization"
},
"familyName": "Santos",
"givenName": "Fred Luciano Neves",
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/s00392-013-0613-y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000868120",
"https://doi.org/10.1007/s00392-013-0613-y"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1136/bmjopen-2016-012799",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001871133"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.parint.2016.12.003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009411185"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pntd.0001498",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013599245"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1086/314723",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018374376"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0161100",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019335015"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0140-6736(15)60692-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021970111"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0140-6736(15)60692-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021970111"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0140-6736(15)60692-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021970111"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1590/s0074-02762009000700021",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026109616"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0169-4758(91)90234-f",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027181294"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0169-4758(91)90234-f",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027181294"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1046/j.1537-2995.2003.00279.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034441572"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/cvi.00489-08",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038773830"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.meegid.2011.12.009",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039104640"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4269/ajtmh.13-0391",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042082529"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.actatropica.2009.07.014",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050351648"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/cvi.00005-09",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050684740"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074358573",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4269/ajtmh.2009.80.410",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1077860979"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079042474",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2450/2015.0040-15",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1079171145"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pntd.0005433",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1084273986"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jcm.00851-17",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1090809456"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.actatropica.2017.09.017",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091886457"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.actatropica.2017.09.017",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091886457"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pntd.0005972",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092132406"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s12879-018-3118-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1103884968",
"https://doi.org/10.1186/s12879-018-3118-5"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s12879-018-3118-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1103884968",
"https://doi.org/10.1186/s12879-018-3118-5"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cmi.2018.06.011",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1104518281"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4269/ajtmh.17-0727",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1107092297"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4269/ajtmh.17-0727",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1107092297"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jcm.00961-18",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1107119359"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jcm.00961-18",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1107119359"
],
"type": "CreativeWork"
}
],
"datePublished": "2019-12",
"datePublishedReg": "2019-12-01",
"description": "BACKGROUND: Chronic Chagas Disease (CD) diagnosis is based on serological methods employing crude, semipurified or recombinant antigens, which may result in low sensitivity or cross-reactivity. To reduce these restrictions, we developed a strategy involving use of molecules containing repetitive fragments of Trypanosoma cruzi conserved proteins. Diagnostic performance of IBMP-8.1 and IBMP-8.4 chimeric antigens (Molecular Biology Institute of Paran\u00e1 - IBMP in Portuguese acronym) was assessed to diagnose T. cruzi-infected and non-infected immigrants living in Barcelona (Spain), a non-endemic setting for Chagas disease.\nMETHODS: Reactivity of IBMP-8.1 and IBMP-8.4 was assessed using an in-house automated ELISA with 347 positive and 331 negative individuals to Chagas disease. Antigenic cross-reactivity was measured with sera samples from pregnant women with Toxoplasma gondii (n\u2009=\u200998) and Zika virus (n\u2009=\u200975) antibodies.\nRESULTS: The area under the curve values was 1 and 0.99 for the IBMP-8.1 and IBMP-8.4 proteins, respectively, demonstrating excellent diagnostic accuracy. The reactivity index was higher for IBMP-8.1 than IBMP-8.4 in positive samples and no significant difference in reactivity index was observed in negative samples. Sensitivity ranged from 99.4% for IBMP-8.1 to 99.1% for IBMP-8.4 and was not statistically different. Specificity for IBMP-8.1 reached 100 and 99.7% for IBMP-8.4, both nearly 100% accurate. No antigenic cross-reactivity was observed and reactivity index was similar to that for negative Chagas disease individuals.\nCONCLUSIONS: Our results showed an outstanding performance of IBMP-8.1 and IBMP-8.4 chimeric antigens by ELISA and suggest both chimeric antigens could also be used for Chagas disease diagnosis in immigrants living in non-endemic settings.",
"genre": "research_article",
"id": "sg:pub.10.1186/s12879-019-3872-z",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1024946",
"issn": [
"1471-2334"
],
"name": "BMC Infectious Diseases",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "19"
}
],
"name": "Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting",
"pagination": "251",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"b2924e2ae7590e632887665813588ff06d03735a0ddf07b0e9e66fb01a5bf1f5"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"30871504"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"100968551"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1186/s12879-019-3872-z"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1112765840"
]
}
],
"sameAs": [
"https://doi.org/10.1186/s12879-019-3872-z",
"https://app.dimensions.ai/details/publication/pub.1112765840"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T13:20",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000368_0000000368/records_78968_00000001.jsonl",
"type": "ScholarlyArticle",
"url": "https://link.springer.com/10.1186%2Fs12879-019-3872-z"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12879-019-3872-z'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12879-019-3872-z'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12879-019-3872-z'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12879-019-3872-z'
This table displays all metadata directly associated to this object as RDF triples.
230 TRIPLES
21 PREDICATES
56 URIs
21 LITERALS
9 BLANK NODES