The use of PCR/Electrospray Ionization-Time-of-Flight-Mass Spectrometry (PCR/ESI-TOF-MS) to detect bacterial and fungal colonization in healthy military service members View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-07-22

AUTHORS

Ryan Vetor, Clinton K. Murray, Katrin Mende, Rachel Melton-Kreft, Kevin S. Akers, Joseph Wenke, Tracy Spirk, Charles Guymon, Wendy Zera, Miriam L. Beckius, Elizabeth R. Schnaubelt, Garth Ehrlich, Todd J. Vento

ABSTRACT

BACKGROUND: The role of microbial colonization in disease is complex. Novel molecular tools to detect colonization offer theoretical improvements over traditional methods. We evaluated PCR/Electrospray Ionization-Time-of-Flight-Mass Spectrometry (PCR/ESI-TOF-MS) as a screening tool to study colonization of healthy military service members. METHODS: We assessed 101 healthy Soldiers using PCR/ESI-TOF-MS on nares, oropharynx, and groin specimens for the presence of gram-positive and gram-negative bacteria (GNB), fungi, and antibiotic resistance genes. A second set of swabs was processed by traditional culture, followed by identification using the BD Phoenix automated system; comparison between PCR/ESI-TOF-MS and culture was carried out only for GNB. RESULTS: Using PCR/ESI-TOF-MS, at least one colonizing organism was found on each individual: mean (SD) number of organisms per subject of 11.8(2.8). The mean number of organisms in the nares, groin and oropharynx was 3.8(1.3), 3.8(1.4) and 4.2(2), respectively. The most commonly detected organisms were aerobic gram-positive bacteria: primarily coagulase-negative Staphylococcus (101 subjects: 341 organisms), Streptococcus pneumoniae (54 subjects: 57 organisms), Staphylococcus aureus (58 subjects: 80 organisms) and Nocardia asteroides (45 subjects: 50 organisms). The mecA gene was found in 96 subjects. The most commonly found GNB was Haemophilus influenzae (20 subjects: 21 organisms) and the most common anaerobe was Propionibacterium acnes (59 subjects). Saccharomyces species (30 subjects) were the most common fungi detected. Only one GNB (nares E. coli) was identified in the same subject by both diagnostic systems. CONCLUSION: PCR/ESI-TOF-MS detected common colonizing organisms and identified more typically-virulent bacteria in asymptomatic, healthy adults. PCR/ESI-TOF-MS appears to be a useful method for detecting bacterial and fungal organisms, but further clinical correlation and validation studies are needed. More... »

PAGES

338

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12879-016-1651-7

DOI

http://dx.doi.org/10.1186/s12879-016-1651-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010340315

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27448413


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adult", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacteria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fungi", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gram-Negative Bacteria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Health", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microbiological Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microbiota", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Military Personnel", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Diagnostic Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pilot Projects", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spectrometry, Mass, Electrospray Ionization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Staphylococcus aureus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Young Adult", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "San Antonio Military Medical Center, JBSA Fort Sam Houston, San Antonio, TX USA", 
          "id": "http://www.grid.ac/institutes/grid.416653.3", 
          "name": [
            "San Antonio Military Medical Center, JBSA Fort Sam Houston, San Antonio, TX USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vetor", 
        "givenName": "Ryan", 
        "id": "sg:person.0646154064.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646154064.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Uniformed Services University of the Health Sciences, Bethesda, Maryland USA", 
          "id": "http://www.grid.ac/institutes/grid.265436.0", 
          "name": [
            "San Antonio Military Medical Center, JBSA Fort Sam Houston, San Antonio, TX USA", 
            "Uniformed Services University of the Health Sciences, Bethesda, Maryland USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Murray", 
        "givenName": "Clinton K.", 
        "id": "sg:person.01345402652.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345402652.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD USA", 
          "id": "http://www.grid.ac/institutes/grid.265436.0", 
          "name": [
            "San Antonio Military Medical Center, JBSA Fort Sam Houston, San Antonio, TX USA", 
            "Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mende", 
        "givenName": "Katrin", 
        "id": "sg:person.0721063242.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721063242.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA USA", 
          "id": "http://www.grid.ac/institutes/grid.280673.8", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Melton-Kreft", 
        "givenName": "Rachel", 
        "id": "sg:person.0771450766.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771450766.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "US Army Institute of Surgical Research, Fort Sam Houston, San Antonio, TX USA", 
          "id": "http://www.grid.ac/institutes/grid.420328.f", 
          "name": [
            "Uniformed Services University of the Health Sciences, Bethesda, Maryland USA", 
            "US Army Institute of Surgical Research, Fort Sam Houston, San Antonio, TX USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Akers", 
        "givenName": "Kevin S.", 
        "id": "sg:person.0652750042.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652750042.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "US Army Institute of Surgical Research, Fort Sam Houston, San Antonio, TX USA", 
          "id": "http://www.grid.ac/institutes/grid.420328.f", 
          "name": [
            "US Army Institute of Surgical Research, Fort Sam Houston, San Antonio, TX USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wenke", 
        "givenName": "Joseph", 
        "id": "sg:person.01272211402.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272211402.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA USA", 
          "id": "http://www.grid.ac/institutes/grid.280673.8", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Spirk", 
        "givenName": "Tracy", 
        "id": "sg:person.01221633600.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221633600.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "US Army Institute of Surgical Research, Fort Sam Houston, San Antonio, TX USA", 
          "id": "http://www.grid.ac/institutes/grid.420328.f", 
          "name": [
            "US Army Institute of Surgical Research, Fort Sam Houston, San Antonio, TX USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Guymon", 
        "givenName": "Charles", 
        "id": "sg:person.01212677036.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01212677036.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD USA", 
          "id": "http://www.grid.ac/institutes/grid.265436.0", 
          "name": [
            "San Antonio Military Medical Center, JBSA Fort Sam Houston, San Antonio, TX USA", 
            "Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zera", 
        "givenName": "Wendy", 
        "id": "sg:person.0672346234.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672346234.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "San Antonio Military Medical Center, JBSA Fort Sam Houston, San Antonio, TX USA", 
          "id": "http://www.grid.ac/institutes/grid.416653.3", 
          "name": [
            "San Antonio Military Medical Center, JBSA Fort Sam Houston, San Antonio, TX USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Beckius", 
        "givenName": "Miriam L.", 
        "id": "sg:person.01327125436.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327125436.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Landstuhl Regional Medical Center, Landstuhl, Germany", 
          "id": "http://www.grid.ac/institutes/grid.415784.b", 
          "name": [
            "Landstuhl Regional Medical Center, Landstuhl, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schnaubelt", 
        "givenName": "Elizabeth R.", 
        "id": "sg:person.0677526141.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677526141.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA USA", 
          "id": "http://www.grid.ac/institutes/grid.280673.8", 
          "name": [
            "Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ehrlich", 
        "givenName": "Garth", 
        "id": "sg:person.01324252605.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324252605.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Infectious Disease Service, (MCHE-MDI), Brooke Army Medical Center, 3551 Roger Brooke Drive, JBSA Fort Sam Houston, 78234 Texas, USA", 
          "id": "http://www.grid.ac/institutes/grid.416653.3", 
          "name": [
            "San Antonio Military Medical Center, JBSA Fort Sam Houston, San Antonio, TX USA", 
            "Uniformed Services University of the Health Sciences, Bethesda, Maryland USA", 
            "Infectious Disease Service, (MCHE-MDI), Brooke Army Medical Center, 3551 Roger Brooke Drive, JBSA Fort Sam Houston, 78234 Texas, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vento", 
        "givenName": "Todd J.", 
        "id": "sg:person.01117162134.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01117162134.33"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1186/1471-2334-13-68", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045834756", 
          "https://doi.org/10.1186/1471-2334-13-68"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro1918", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038352578", 
          "https://doi.org/10.1038/nrmicro1918"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2334-12-252", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029911813", 
          "https://doi.org/10.1186/1471-2334-12-252"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2334-10-285", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019219352", 
          "https://doi.org/10.1186/1471-2334-10-285"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2008-9-6-225", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041268307", 
          "https://doi.org/10.1186/gb-2008-9-6-225"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro1871", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007958178", 
          "https://doi.org/10.1038/nrmicro1871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2334-10-304", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021792783", 
          "https://doi.org/10.1186/1471-2334-10-304"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-07-22", 
    "datePublishedReg": "2016-07-22", 
    "description": "BACKGROUND: The role of microbial colonization in disease is complex. Novel molecular tools to detect colonization offer theoretical improvements over traditional methods. We evaluated PCR/Electrospray Ionization-Time-of-Flight-Mass Spectrometry (PCR/ESI-TOF-MS) as a screening tool to study colonization of healthy military service members.\nMETHODS: We assessed 101 healthy Soldiers using PCR/ESI-TOF-MS on nares, oropharynx, and groin specimens for the presence of gram-positive and gram-negative bacteria (GNB), fungi, and antibiotic resistance genes. A second set of swabs was processed by traditional culture, followed by identification using the BD Phoenix automated system; comparison between PCR/ESI-TOF-MS and culture was carried out only for GNB.\nRESULTS: Using PCR/ESI-TOF-MS, at least one colonizing organism was found on each individual: mean (SD) number of organisms per subject of 11.8(2.8). The mean number of organisms in the nares, groin and oropharynx was 3.8(1.3), 3.8(1.4) and 4.2(2), respectively. The most commonly detected organisms were aerobic gram-positive bacteria: primarily coagulase-negative Staphylococcus (101 subjects: 341 organisms), Streptococcus pneumoniae (54 subjects: 57 organisms), Staphylococcus aureus (58 subjects: 80 organisms) and Nocardia asteroides (45 subjects: 50 organisms). The mecA gene was found in 96 subjects. The most commonly found GNB was Haemophilus influenzae (20 subjects: 21 organisms) and the most common anaerobe was Propionibacterium acnes (59 subjects). Saccharomyces species (30 subjects) were the most common fungi detected. Only one GNB (nares E. coli) was identified in the same subject by both diagnostic systems.\nCONCLUSION: PCR/ESI-TOF-MS detected common colonizing organisms and identified more typically-virulent bacteria in asymptomatic, healthy adults. PCR/ESI-TOF-MS appears to be a useful method for detecting bacterial and fungal organisms, but further clinical correlation and validation studies are needed.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/s12879-016-1651-7", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1024946", 
        "issn": [
          "1471-2334"
        ], 
        "name": "BMC Infectious Diseases", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "16"
      }
    ], 
    "keywords": [
      "novel molecular tools", 
      "ionization time", 
      "flight mass spectrometry", 
      "number of organisms", 
      "molecular tools", 
      "antibiotic resistance genes", 
      "resistance genes", 
      "fungal colonization", 
      "organisms", 
      "microbial colonization", 
      "negative bacteria", 
      "positive bacteria", 
      "colonization", 
      "genes", 
      "common fungi", 
      "bacteria", 
      "virulent bacteria", 
      "fungi", 
      "electrospray ionization time", 
      "fungal organisms", 
      "colonizing organisms", 
      "common colonizing organisms", 
      "Saccharomyces", 
      "ESI-TOF", 
      "members", 
      "military service members", 
      "spectrometry", 
      "culture", 
      "service members", 
      "anaerobes", 
      "MS", 
      "coagulase-negative staphylococci", 
      "Staphylococcus aureus", 
      "identification", 
      "Haemophilus influenzae", 
      "clinical correlation", 
      "common anaerobes", 
      "role", 
      "BD Phoenix", 
      "healthy adults", 
      "Streptococcus pneumoniae", 
      "Propionibacterium acnes", 
      "healthy soldiers", 
      "Nocardia asteroides", 
      "mecA gene", 
      "mean number", 
      "oropharynx", 
      "number", 
      "asteroides", 
      "nares", 
      "GNB", 
      "validation study", 
      "tool", 
      "second set", 
      "subjects", 
      "staphylococci", 
      "same subjects", 
      "presence", 
      "individuals", 
      "aureus", 
      "pneumoniae", 
      "disease", 
      "specimens", 
      "useful method", 
      "acne", 
      "groin", 
      "influenzae", 
      "swabs", 
      "system", 
      "adults", 
      "study", 
      "comparison", 
      "set", 
      "correlation", 
      "traditional methods", 
      "Phoenix", 
      "diagnostic system", 
      "improvement", 
      "use", 
      "method", 
      "soldiers", 
      "traditional culture", 
      "theoretical improvements", 
      "PCR/Electrospray Ionization-Time", 
      "healthy military service members", 
      "PCR/ESI-TOF"
    ], 
    "name": "The use of PCR/Electrospray Ionization-Time-of-Flight-Mass Spectrometry (PCR/ESI-TOF-MS) to detect bacterial and fungal colonization in healthy military service members", 
    "pagination": "338", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1010340315"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12879-016-1651-7"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27448413"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12879-016-1651-7", 
      "https://app.dimensions.ai/details/publication/pub.1010340315"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-11-01T18:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/article/article_705.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/s12879-016-1651-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12879-016-1651-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12879-016-1651-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12879-016-1651-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12879-016-1651-7'


 

This table displays all metadata directly associated to this object as RDF triples.

362 TRIPLES      22 PREDICATES      140 URIs      123 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12879-016-1651-7 schema:about N046267ecbc234f419b66db05ca6430e5
2 N074ef025b47542ffbb7488cefa60bb78
3 N0bfbd634b1314d4c9c02280cd24767d9
4 N232566cca87745ff9f15cdac490b80d6
5 N25514a7aa714483998c9fbe016df9ed7
6 N3925f8b3561442e590d5a39bab52458a
7 N3a3599f5e3c5442bb5413c9b0080a5d8
8 N53a682e19cea4d499aa88847db59b393
9 N5efcc6f41470470a915b742e118fe46a
10 N7f409de0a3c54683b2b9853d6d93996e
11 N846e3914c01642c885fb47e2cfbe8dfe
12 N87f7cd4b69654d0fb416cd8cadef7538
13 N95252d0da58b44aea4e9033bcfa83b0f
14 Na3bf173980514ca9a117387efa245be8
15 Na96430057eab45349fbe4d210a33437a
16 Nc41d67d3819143a5b237b54601737b3e
17 Nc8ec27367d1e4d03892658f06cb514b0
18 Nede2ec88d84f4253be9d3ec2647cfbdb
19 Nfd278a5e64ed46f5938405cf5b09d018
20 anzsrc-for:06
21 anzsrc-for:0605
22 anzsrc-for:11
23 anzsrc-for:1108
24 schema:author Ndea225a925514f7c8c96e02a55c4584d
25 schema:citation sg:pub.10.1038/nrmicro1871
26 sg:pub.10.1038/nrmicro1918
27 sg:pub.10.1186/1471-2334-10-285
28 sg:pub.10.1186/1471-2334-10-304
29 sg:pub.10.1186/1471-2334-12-252
30 sg:pub.10.1186/1471-2334-13-68
31 sg:pub.10.1186/gb-2008-9-6-225
32 schema:datePublished 2016-07-22
33 schema:datePublishedReg 2016-07-22
34 schema:description BACKGROUND: The role of microbial colonization in disease is complex. Novel molecular tools to detect colonization offer theoretical improvements over traditional methods. We evaluated PCR/Electrospray Ionization-Time-of-Flight-Mass Spectrometry (PCR/ESI-TOF-MS) as a screening tool to study colonization of healthy military service members. METHODS: We assessed 101 healthy Soldiers using PCR/ESI-TOF-MS on nares, oropharynx, and groin specimens for the presence of gram-positive and gram-negative bacteria (GNB), fungi, and antibiotic resistance genes. A second set of swabs was processed by traditional culture, followed by identification using the BD Phoenix automated system; comparison between PCR/ESI-TOF-MS and culture was carried out only for GNB. RESULTS: Using PCR/ESI-TOF-MS, at least one colonizing organism was found on each individual: mean (SD) number of organisms per subject of 11.8(2.8). The mean number of organisms in the nares, groin and oropharynx was 3.8(1.3), 3.8(1.4) and 4.2(2), respectively. The most commonly detected organisms were aerobic gram-positive bacteria: primarily coagulase-negative Staphylococcus (101 subjects: 341 organisms), Streptococcus pneumoniae (54 subjects: 57 organisms), Staphylococcus aureus (58 subjects: 80 organisms) and Nocardia asteroides (45 subjects: 50 organisms). The mecA gene was found in 96 subjects. The most commonly found GNB was Haemophilus influenzae (20 subjects: 21 organisms) and the most common anaerobe was Propionibacterium acnes (59 subjects). Saccharomyces species (30 subjects) were the most common fungi detected. Only one GNB (nares E. coli) was identified in the same subject by both diagnostic systems. CONCLUSION: PCR/ESI-TOF-MS detected common colonizing organisms and identified more typically-virulent bacteria in asymptomatic, healthy adults. PCR/ESI-TOF-MS appears to be a useful method for detecting bacterial and fungal organisms, but further clinical correlation and validation studies are needed.
35 schema:genre article
36 schema:inLanguage en
37 schema:isAccessibleForFree true
38 schema:isPartOf Nab4717cdde464429938b151c1dfb8e92
39 Nf32fac6b0e7842a8a92cec7b9a65d1bc
40 sg:journal.1024946
41 schema:keywords BD Phoenix
42 ESI-TOF
43 GNB
44 Haemophilus influenzae
45 MS
46 Nocardia asteroides
47 PCR/ESI-TOF
48 PCR/Electrospray Ionization-Time
49 Phoenix
50 Propionibacterium acnes
51 Saccharomyces
52 Staphylococcus aureus
53 Streptococcus pneumoniae
54 acne
55 adults
56 anaerobes
57 antibiotic resistance genes
58 asteroides
59 aureus
60 bacteria
61 clinical correlation
62 coagulase-negative staphylococci
63 colonization
64 colonizing organisms
65 common anaerobes
66 common colonizing organisms
67 common fungi
68 comparison
69 correlation
70 culture
71 diagnostic system
72 disease
73 electrospray ionization time
74 flight mass spectrometry
75 fungal colonization
76 fungal organisms
77 fungi
78 genes
79 groin
80 healthy adults
81 healthy military service members
82 healthy soldiers
83 identification
84 improvement
85 individuals
86 influenzae
87 ionization time
88 mean number
89 mecA gene
90 members
91 method
92 microbial colonization
93 military service members
94 molecular tools
95 nares
96 negative bacteria
97 novel molecular tools
98 number
99 number of organisms
100 organisms
101 oropharynx
102 pneumoniae
103 positive bacteria
104 presence
105 resistance genes
106 role
107 same subjects
108 second set
109 service members
110 set
111 soldiers
112 specimens
113 spectrometry
114 staphylococci
115 study
116 subjects
117 swabs
118 system
119 theoretical improvements
120 tool
121 traditional culture
122 traditional methods
123 use
124 useful method
125 validation study
126 virulent bacteria
127 schema:name The use of PCR/Electrospray Ionization-Time-of-Flight-Mass Spectrometry (PCR/ESI-TOF-MS) to detect bacterial and fungal colonization in healthy military service members
128 schema:pagination 338
129 schema:productId N0c9b219d47794bf5977d41a0d3ce3aec
130 N21a72df3b9ed42d8955341d1dbe3816e
131 N42a39618c88743bba8bebf1cf144aba1
132 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010340315
133 https://doi.org/10.1186/s12879-016-1651-7
134 schema:sdDatePublished 2021-11-01T18:27
135 schema:sdLicense https://scigraph.springernature.com/explorer/license/
136 schema:sdPublisher Nc1ff038b44424923865aae977abe1e0b
137 schema:url https://doi.org/10.1186/s12879-016-1651-7
138 sgo:license sg:explorer/license/
139 sgo:sdDataset articles
140 rdf:type schema:ScholarlyArticle
141 N046267ecbc234f419b66db05ca6430e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Staphylococcus aureus
143 rdf:type schema:DefinedTerm
144 N074ef025b47542ffbb7488cefa60bb78 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Polymerase Chain Reaction
146 rdf:type schema:DefinedTerm
147 N0bfbd634b1314d4c9c02280cd24767d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Microbiological Techniques
149 rdf:type schema:DefinedTerm
150 N0c9b219d47794bf5977d41a0d3ce3aec schema:name doi
151 schema:value 10.1186/s12879-016-1651-7
152 rdf:type schema:PropertyValue
153 N12b9b972aef84f69819dad68393a1d0d rdf:first sg:person.0677526141.30
154 rdf:rest N2bbc08dc894d421e8ab828e5f4c645df
155 N198158b066494e14ad65e506536e626f rdf:first sg:person.01221633600.74
156 rdf:rest N5ed3ec87c0b64e018708bae837603575
157 N21a72df3b9ed42d8955341d1dbe3816e schema:name pubmed_id
158 schema:value 27448413
159 rdf:type schema:PropertyValue
160 N232566cca87745ff9f15cdac490b80d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Fungi
162 rdf:type schema:DefinedTerm
163 N25122dc16526415186cfb64ec341be45 rdf:first sg:person.0672346234.32
164 rdf:rest N9e8ef4f1e4fe489db70d76ae87302468
165 N25514a7aa714483998c9fbe016df9ed7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
167 rdf:type schema:DefinedTerm
168 N2bbc08dc894d421e8ab828e5f4c645df rdf:first sg:person.01324252605.57
169 rdf:rest N82ee8df9b5244a26b6ebf71ea4dca8a7
170 N3925f8b3561442e590d5a39bab52458a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Molecular Diagnostic Techniques
172 rdf:type schema:DefinedTerm
173 N3a3599f5e3c5442bb5413c9b0080a5d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
174 schema:name Gram-Negative Bacteria
175 rdf:type schema:DefinedTerm
176 N42a39618c88743bba8bebf1cf144aba1 schema:name dimensions_id
177 schema:value pub.1010340315
178 rdf:type schema:PropertyValue
179 N53a682e19cea4d499aa88847db59b393 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name Microbiota
181 rdf:type schema:DefinedTerm
182 N588685598ece41308aefcee5dce246f3 rdf:first sg:person.01272211402.70
183 rdf:rest N198158b066494e14ad65e506536e626f
184 N5ed3ec87c0b64e018708bae837603575 rdf:first sg:person.01212677036.05
185 rdf:rest N25122dc16526415186cfb64ec341be45
186 N5efcc6f41470470a915b742e118fe46a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
187 schema:name Pilot Projects
188 rdf:type schema:DefinedTerm
189 N788f043e539d47f29b5c2632182d2882 rdf:first sg:person.0771450766.67
190 rdf:rest Nf7f120b62971489fb51d137c112a11ed
191 N7f409de0a3c54683b2b9853d6d93996e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Military Personnel
193 rdf:type schema:DefinedTerm
194 N82ee8df9b5244a26b6ebf71ea4dca8a7 rdf:first sg:person.01117162134.33
195 rdf:rest rdf:nil
196 N846e3914c01642c885fb47e2cfbe8dfe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
197 schema:name Male
198 rdf:type schema:DefinedTerm
199 N87f7cd4b69654d0fb416cd8cadef7538 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
200 schema:name Health
201 rdf:type schema:DefinedTerm
202 N95252d0da58b44aea4e9033bcfa83b0f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
203 schema:name Young Adult
204 rdf:type schema:DefinedTerm
205 N95a9a2a6f0024c3fa3fb40f8b5c2ba83 rdf:first sg:person.01345402652.14
206 rdf:rest Nd5bec8afe40443b19da91f3220f6eb01
207 N9e8ef4f1e4fe489db70d76ae87302468 rdf:first sg:person.01327125436.78
208 rdf:rest N12b9b972aef84f69819dad68393a1d0d
209 Na3bf173980514ca9a117387efa245be8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
210 schema:name Female
211 rdf:type schema:DefinedTerm
212 Na96430057eab45349fbe4d210a33437a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
213 schema:name Adult
214 rdf:type schema:DefinedTerm
215 Nab4717cdde464429938b151c1dfb8e92 schema:volumeNumber 16
216 rdf:type schema:PublicationVolume
217 Nc1ff038b44424923865aae977abe1e0b schema:name Springer Nature - SN SciGraph project
218 rdf:type schema:Organization
219 Nc41d67d3819143a5b237b54601737b3e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
220 schema:name Escherichia coli
221 rdf:type schema:DefinedTerm
222 Nc8ec27367d1e4d03892658f06cb514b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
223 schema:name Spectrometry, Mass, Electrospray Ionization
224 rdf:type schema:DefinedTerm
225 Nd5bec8afe40443b19da91f3220f6eb01 rdf:first sg:person.0721063242.21
226 rdf:rest N788f043e539d47f29b5c2632182d2882
227 Ndea225a925514f7c8c96e02a55c4584d rdf:first sg:person.0646154064.48
228 rdf:rest N95a9a2a6f0024c3fa3fb40f8b5c2ba83
229 Nede2ec88d84f4253be9d3ec2647cfbdb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
230 schema:name Bacteria
231 rdf:type schema:DefinedTerm
232 Nf32fac6b0e7842a8a92cec7b9a65d1bc schema:issueNumber 1
233 rdf:type schema:PublicationIssue
234 Nf7f120b62971489fb51d137c112a11ed rdf:first sg:person.0652750042.34
235 rdf:rest N588685598ece41308aefcee5dce246f3
236 Nfd278a5e64ed46f5938405cf5b09d018 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
237 schema:name Humans
238 rdf:type schema:DefinedTerm
239 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
240 schema:name Biological Sciences
241 rdf:type schema:DefinedTerm
242 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
243 schema:name Microbiology
244 rdf:type schema:DefinedTerm
245 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
246 schema:name Medical and Health Sciences
247 rdf:type schema:DefinedTerm
248 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
249 schema:name Medical Microbiology
250 rdf:type schema:DefinedTerm
251 sg:journal.1024946 schema:issn 1471-2334
252 schema:name BMC Infectious Diseases
253 schema:publisher Springer Nature
254 rdf:type schema:Periodical
255 sg:person.01117162134.33 schema:affiliation grid-institutes:grid.416653.3
256 schema:familyName Vento
257 schema:givenName Todd J.
258 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01117162134.33
259 rdf:type schema:Person
260 sg:person.01212677036.05 schema:affiliation grid-institutes:grid.420328.f
261 schema:familyName Guymon
262 schema:givenName Charles
263 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01212677036.05
264 rdf:type schema:Person
265 sg:person.01221633600.74 schema:affiliation grid-institutes:grid.280673.8
266 schema:familyName Spirk
267 schema:givenName Tracy
268 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221633600.74
269 rdf:type schema:Person
270 sg:person.01272211402.70 schema:affiliation grid-institutes:grid.420328.f
271 schema:familyName Wenke
272 schema:givenName Joseph
273 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272211402.70
274 rdf:type schema:Person
275 sg:person.01324252605.57 schema:affiliation grid-institutes:grid.280673.8
276 schema:familyName Ehrlich
277 schema:givenName Garth
278 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324252605.57
279 rdf:type schema:Person
280 sg:person.01327125436.78 schema:affiliation grid-institutes:grid.416653.3
281 schema:familyName Beckius
282 schema:givenName Miriam L.
283 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327125436.78
284 rdf:type schema:Person
285 sg:person.01345402652.14 schema:affiliation grid-institutes:grid.265436.0
286 schema:familyName Murray
287 schema:givenName Clinton K.
288 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345402652.14
289 rdf:type schema:Person
290 sg:person.0646154064.48 schema:affiliation grid-institutes:grid.416653.3
291 schema:familyName Vetor
292 schema:givenName Ryan
293 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646154064.48
294 rdf:type schema:Person
295 sg:person.0652750042.34 schema:affiliation grid-institutes:grid.420328.f
296 schema:familyName Akers
297 schema:givenName Kevin S.
298 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652750042.34
299 rdf:type schema:Person
300 sg:person.0672346234.32 schema:affiliation grid-institutes:grid.265436.0
301 schema:familyName Zera
302 schema:givenName Wendy
303 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672346234.32
304 rdf:type schema:Person
305 sg:person.0677526141.30 schema:affiliation grid-institutes:grid.415784.b
306 schema:familyName Schnaubelt
307 schema:givenName Elizabeth R.
308 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677526141.30
309 rdf:type schema:Person
310 sg:person.0721063242.21 schema:affiliation grid-institutes:grid.265436.0
311 schema:familyName Mende
312 schema:givenName Katrin
313 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721063242.21
314 rdf:type schema:Person
315 sg:person.0771450766.67 schema:affiliation grid-institutes:grid.280673.8
316 schema:familyName Melton-Kreft
317 schema:givenName Rachel
318 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771450766.67
319 rdf:type schema:Person
320 sg:pub.10.1038/nrmicro1871 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007958178
321 https://doi.org/10.1038/nrmicro1871
322 rdf:type schema:CreativeWork
323 sg:pub.10.1038/nrmicro1918 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038352578
324 https://doi.org/10.1038/nrmicro1918
325 rdf:type schema:CreativeWork
326 sg:pub.10.1186/1471-2334-10-285 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019219352
327 https://doi.org/10.1186/1471-2334-10-285
328 rdf:type schema:CreativeWork
329 sg:pub.10.1186/1471-2334-10-304 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021792783
330 https://doi.org/10.1186/1471-2334-10-304
331 rdf:type schema:CreativeWork
332 sg:pub.10.1186/1471-2334-12-252 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029911813
333 https://doi.org/10.1186/1471-2334-12-252
334 rdf:type schema:CreativeWork
335 sg:pub.10.1186/1471-2334-13-68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045834756
336 https://doi.org/10.1186/1471-2334-13-68
337 rdf:type schema:CreativeWork
338 sg:pub.10.1186/gb-2008-9-6-225 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041268307
339 https://doi.org/10.1186/gb-2008-9-6-225
340 rdf:type schema:CreativeWork
341 grid-institutes:grid.265436.0 schema:alternateName Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD USA
342 Uniformed Services University of the Health Sciences, Bethesda, Maryland USA
343 schema:name Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD USA
344 San Antonio Military Medical Center, JBSA Fort Sam Houston, San Antonio, TX USA
345 Uniformed Services University of the Health Sciences, Bethesda, Maryland USA
346 rdf:type schema:Organization
347 grid-institutes:grid.280673.8 schema:alternateName Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA USA
348 schema:name Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA USA
349 rdf:type schema:Organization
350 grid-institutes:grid.415784.b schema:alternateName Landstuhl Regional Medical Center, Landstuhl, Germany
351 schema:name Landstuhl Regional Medical Center, Landstuhl, Germany
352 rdf:type schema:Organization
353 grid-institutes:grid.416653.3 schema:alternateName Infectious Disease Service, (MCHE-MDI), Brooke Army Medical Center, 3551 Roger Brooke Drive, JBSA Fort Sam Houston, 78234 Texas, USA
354 San Antonio Military Medical Center, JBSA Fort Sam Houston, San Antonio, TX USA
355 schema:name Infectious Disease Service, (MCHE-MDI), Brooke Army Medical Center, 3551 Roger Brooke Drive, JBSA Fort Sam Houston, 78234 Texas, USA
356 San Antonio Military Medical Center, JBSA Fort Sam Houston, San Antonio, TX USA
357 Uniformed Services University of the Health Sciences, Bethesda, Maryland USA
358 rdf:type schema:Organization
359 grid-institutes:grid.420328.f schema:alternateName US Army Institute of Surgical Research, Fort Sam Houston, San Antonio, TX USA
360 schema:name US Army Institute of Surgical Research, Fort Sam Houston, San Antonio, TX USA
361 Uniformed Services University of the Health Sciences, Bethesda, Maryland USA
362 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...