Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems? View Full Text


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Article Info

DATE

2019-06-21

AUTHORS

Annelie Gutsch, Kjell Sergeant, Els Keunen, Els Prinsen, Gea Guerriero, Jenny Renaut, Jean-Francois Hausman, Ann Cuypers

ABSTRACT

BackgroundThe heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences. It is unessential and harmful to all life forms. The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure. In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd. Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.ResultsSeveral proteins determining for the cell wall structure changed in abundance. Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd. Although a higher abundance and enzymatic activity of pectin methylesterase was detected, the total pectin methylation was not affected.ConclusionsAn increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone. Probably, the exclusion of Cd from the cell wall and apoplast limits the entry of the heavy metal into the symplast and is an important factor during tolerance acquisition. More... »

PAGES

271

References to SciGraph publications

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  • 2007-11-07. Xyloglucan−pectin linkages are formed intra-protoplasmically, contribute to wall-assembly, and remain stable in the cell wall in PLANTA
  • 2007-12-08. Dynamics of phenolic acids and lignin accumulation in metal-treated Matricaria chamomilla roots in PLANT CELL REPORTS
  • 2015-07-07. The Medicago sativa gene index 1.2: a web-accessible gene expression atlas for investigating expression differences between Medicago sativa subspecies in BMC GENOMICS
  • 2015-07-14. Tuning of pectin methylesterification: consequences for cell wall biomechanics and development in PLANTA
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1186/s12870-019-1859-y

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    http://dx.doi.org/10.1186/s12870-019-1859-y

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/31226937


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    34 schema:description BackgroundThe heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences. It is unessential and harmful to all life forms. The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure. In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd. Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition.ResultsSeveral proteins determining for the cell wall structure changed in abundance. Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd. Although a higher abundance and enzymatic activity of pectin methylesterase was detected, the total pectin methylation was not affected.ConclusionsAn increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone. Probably, the exclusion of Cd from the cell wall and apoplast limits the entry of the heavy metal into the symplast and is an important factor during tolerance acquisition.
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    42 Cd exposure
    43 ConclusionsAn
    44 Medicago sativa
    45 abundance
    46 acid backbone
    47 acquisition
    48 activity
    49 analysis
    50 anthropogenic influences
    51 apoplast
    52 backbone
    53 barriers
    54 cadmium
    55 cell wall
    56 cell wall composition
    57 cell wall structure
    58 changes
    59 chromatography
    60 composition
    61 current study
    62 data
    63 entry
    64 environment
    65 environmental stress
    66 enzymatic activity
    67 exclusion
    68 exclusion of Cd
    69 exposure
    70 exposure influence
    71 expression analysis
    72 factors
    73 form
    74 galacturonic acid backbone
    75 gene expression analysis
    76 heavy metal cadmium
    77 heavy metals
    78 high abundance
    79 important factor
    80 influence
    81 life forms
    82 liquid chromatography
    83 long-term exposure
    84 mass spectrometry
    85 metal cadmium
    86 metals
    87 methylation
    88 methylesterase
    89 pectic polysaccharides
    90 pectin methylation
    91 pectin methylesterase
    92 physical barrier
    93 plant cell walls
    94 polysaccharides
    95 presence
    96 protein
    97 protein analysis
    98 quantitative protein analysis
    99 response
    100 sativa
    101 spectrometry
    102 stem
    103 stress
    104 structural changes
    105 structure
    106 study
    107 symplast
    108 targeted gene expression analysis
    109 tolerance acquisition
    110 wall
    111 wall composition
    112 wall structure
    113 xylogalacturonan
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