Identification of candidate genes responsible for the susceptibility of apple (Malus × domestica Borkh.) to Alternaria blotch View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-04-08

AUTHORS

Shigeki Moriya, Shingo Terakami, Kazuma Okada, Taku Shimizu, Yoshihiko Adachi, Yuichi Katayose, Hiroko Fujisawa, Jianzhon Wu, Hiroyuki Kanamori, Toshiya Yamamoto, Kazuyuki Abe

ABSTRACT

BackgroundThe mechanism underlying the interaction between host plant and host-selective toxin (HST)-producing Alternaria alternata during infection is of particular interest for sustainable crop production. Alternaria blotch of apple (Malus × domestica Borkh.) caused by A. alternata apple pathotype is a major disease particularly in East Asia, which is the largest producer of apples globally. A single dominant gene, Alt, controls the susceptibility of the apple cultivar ‘Delicious’ to Alternaria blotch. In this study, we fine mapped the Alt locus and characterized three potential candidate genes.ResultsWe used 797 F1 individuals derived from 15 crosses between apple accessions susceptible (Alt/alt) and resistant (alt/alt) to Alternaria blotch to construct physical and genetic maps of the Alt locus located on the top of chromosome 11. Susceptible accessions were derived from ‘Delicious.’ To fine map the Alt locus, we constructed a BAC library of ‘Starking Delicious,’ a sport of ‘Delicious,’ and used graphical genotyping to delimit the Alt locus to a region of 43 kb. Three genes predicted within the candidate Alt region were potentially involved in plant defense response, among which the gene encoding a coiled coil-nucleotide binding-leucine rich repeat (CC-NB-LRR) type disease resistance protein was the most promising. Moreover, a 12-bp insertion was uniquely identified in the 5′ untranslated region of the Alt-associated allele of this gene, the presence or absence of which co-segregated with the susceptibility or resistance to A. alternata apple pathotype, respectively, among 43 tested cultivars including old ones and founders of modern apple breeding.ConclusionA disease resistance protein has been suggested as a determinant of susceptibility/resistance to HST-producing A. alternata for the first time. Our finding provides new insight into the mechanism of HST-mediated disease control used by A. alternata against host plants. More... »

PAGES

132

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12870-019-1737-7

DOI

http://dx.doi.org/10.1186/s12870-019-1737-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1113300161

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30961541


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alternaria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Resistance", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Library", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leucine-Rich Repeat Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Malus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Diseases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Apple Research Station, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 92-24 Shimokuriyagawa Nabeyashiki, 020-0123, Morioka, Iwate, Japan", 
          "id": "http://www.grid.ac/institutes/grid.482552.c", 
          "name": [
            "Apple Research Station, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 92-24 Shimokuriyagawa Nabeyashiki, 020-0123, Morioka, Iwate, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Moriya", 
        "givenName": "Shigeki", 
        "id": "sg:person.01067604123.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067604123.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Fruit Tree and Tea Science, NARO, 2-1 Fujimoto, 305-8604, Tsukuba, Ibaraki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.482552.c", 
          "name": [
            "Institute of Fruit Tree and Tea Science, NARO, 2-1 Fujimoto, 305-8604, Tsukuba, Ibaraki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Terakami", 
        "givenName": "Shingo", 
        "id": "sg:person.015205461257.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015205461257.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Apple Research Station, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 92-24 Shimokuriyagawa Nabeyashiki, 020-0123, Morioka, Iwate, Japan", 
          "id": "http://www.grid.ac/institutes/grid.482552.c", 
          "name": [
            "Apple Research Station, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 92-24 Shimokuriyagawa Nabeyashiki, 020-0123, Morioka, Iwate, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Okada", 
        "givenName": "Kazuma", 
        "id": "sg:person.01021470723.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021470723.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Apple Research Station, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 92-24 Shimokuriyagawa Nabeyashiki, 020-0123, Morioka, Iwate, Japan", 
          "id": "http://www.grid.ac/institutes/grid.482552.c", 
          "name": [
            "Apple Research Station, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 92-24 Shimokuriyagawa Nabeyashiki, 020-0123, Morioka, Iwate, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shimizu", 
        "givenName": "Taku", 
        "id": "sg:person.015235550023.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015235550023.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Citrus Research Station, Institute of Fruit Tree and Tea Science, NARO, 485-6 Okitsunaka-cho, Shimizu, 424-0284, Shizuoka City, Shizuoka, Japan", 
          "id": "http://www.grid.ac/institutes/grid.482552.c", 
          "name": [
            "Citrus Research Station, Institute of Fruit Tree and Tea Science, NARO, 485-6 Okitsunaka-cho, Shimizu, 424-0284, Shizuoka City, Shizuoka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Adachi", 
        "givenName": "Yoshihiko", 
        "id": "sg:person.0710322373.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0710322373.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Advanced Genomics Breeding Section, Institute of Crop Science, NARO, 1-2 Ohwashi, 305-8634, Tsukuba, Ibaraki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.419573.d", 
          "name": [
            "Advanced Genomics Breeding Section, Institute of Crop Science, NARO, 1-2 Ohwashi, 305-8634, Tsukuba, Ibaraki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Katayose", 
        "givenName": "Yuichi", 
        "id": "sg:person.0647572154.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647572154.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Advanced Genomics Breeding Section, Institute of Crop Science, NARO, 1-2 Ohwashi, 305-8634, Tsukuba, Ibaraki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.419573.d", 
          "name": [
            "Advanced Genomics Breeding Section, Institute of Crop Science, NARO, 1-2 Ohwashi, 305-8634, Tsukuba, Ibaraki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fujisawa", 
        "givenName": "Hiroko", 
        "id": "sg:person.01110271121.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110271121.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Advanced Genomics Breeding Section, Institute of Crop Science, NARO, 1-2 Ohwashi, 305-8634, Tsukuba, Ibaraki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.419573.d", 
          "name": [
            "Advanced Genomics Breeding Section, Institute of Crop Science, NARO, 1-2 Ohwashi, 305-8634, Tsukuba, Ibaraki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wu", 
        "givenName": "Jianzhon", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Advanced Genomics Breeding Section, Institute of Crop Science, NARO, 1-2 Ohwashi, 305-8634, Tsukuba, Ibaraki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.419573.d", 
          "name": [
            "Advanced Genomics Breeding Section, Institute of Crop Science, NARO, 1-2 Ohwashi, 305-8634, Tsukuba, Ibaraki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kanamori", 
        "givenName": "Hiroyuki", 
        "id": "sg:person.0657614121.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657614121.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Fruit Tree and Tea Science, NARO, 2-1 Fujimoto, 305-8604, Tsukuba, Ibaraki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.482552.c", 
          "name": [
            "Institute of Fruit Tree and Tea Science, NARO, 2-1 Fujimoto, 305-8604, Tsukuba, Ibaraki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamamoto", 
        "givenName": "Toshiya", 
        "id": "sg:person.0623525444.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623525444.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Apple Research Station, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 92-24 Shimokuriyagawa Nabeyashiki, 020-0123, Morioka, Iwate, Japan", 
          "id": "http://www.grid.ac/institutes/grid.482552.c", 
          "name": [
            "Apple Research Station, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 92-24 Shimokuriyagawa Nabeyashiki, 020-0123, Morioka, Iwate, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Abe", 
        "givenName": "Kazuyuki", 
        "id": "sg:person.01204032523.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204032523.63"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/ng.654", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025100649", 
          "https://doi.org/10.1038/ng.654"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cdd.2011.37", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030921204", 
          "https://doi.org/10.1038/cdd.2011.37"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2105-9-253", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049665949", 
          "https://doi.org/10.1186/1471-2105-9-253"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10327-013-0498-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040845188", 
          "https://doi.org/10.1007/s10327-013-0498-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11295-006-0045-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010221339", 
          "https://doi.org/10.1007/s11295-006-0045-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10327-008-0089-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014897425", 
          "https://doi.org/10.1007/s10327-008-0089-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002940100233", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030269188", 
          "https://doi.org/10.1007/s002940100233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01184", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046673491", 
          "https://doi.org/10.1038/nature01184"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10658-016-0866-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025625115", 
          "https://doi.org/10.1007/s10658-016-0866-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng.3886", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085864660", 
          "https://doi.org/10.1038/ng.3886"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-04-08", 
    "datePublishedReg": "2019-04-08", 
    "description": "BackgroundThe mechanism underlying the interaction between host plant and host-selective toxin (HST)-producing Alternaria alternata during infection is of particular interest for sustainable crop production. Alternaria blotch of apple (Malus \u00d7 domestica Borkh.) caused by A. alternata apple pathotype is a major disease particularly in East Asia, which is the largest producer of apples globally. A single dominant gene, Alt, controls the susceptibility of the apple cultivar \u2018Delicious\u2019 to Alternaria blotch. In this study, we fine mapped the Alt locus and characterized three potential candidate genes.ResultsWe used 797 F1 individuals derived from 15 crosses between apple accessions susceptible (Alt/alt) and resistant (alt/alt) to Alternaria blotch to construct physical and genetic maps of the Alt locus located on the top of chromosome 11. Susceptible accessions were derived from \u2018Delicious.\u2019 To fine map the Alt locus, we constructed a BAC library of \u2018Starking Delicious,\u2019 a sport of \u2018Delicious,\u2019 and used graphical genotyping to delimit the Alt locus to a region of 43\u2009kb. Three genes predicted within the candidate Alt region were potentially involved in plant defense response, among which the gene encoding a coiled coil-nucleotide binding-leucine rich repeat (CC-NB-LRR) type disease resistance protein was the most promising. Moreover, a 12-bp insertion was uniquely identified in the 5\u2032 untranslated region of the Alt-associated allele of this gene, the presence or absence of which co-segregated with the susceptibility or resistance to A. alternata apple pathotype, respectively, among 43 tested cultivars including old ones and founders of modern apple breeding.ConclusionA disease resistance protein has been suggested as a determinant of susceptibility/resistance to HST-producing A. alternata for the first time. Our finding provides new insight into the mechanism of HST-mediated disease control used by A. alternata against host plants.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/s12870-019-1737-7", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1024638", 
        "issn": [
          "1471-2229"
        ], 
        "name": "BMC Plant Biology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "19"
      }
    ], 
    "keywords": [
      "disease resistance proteins", 
      "Alt locus", 
      "A. alternata apple pathotype", 
      "host plants", 
      "candidate genes", 
      "apple pathotype", 
      "plant defense responses", 
      "A. alternata", 
      "single dominant gene", 
      "host-selective toxin", 
      "resistance protein", 
      "Alternaria blotch", 
      "potential candidate genes", 
      "sustainable crop production", 
      "genetic map", 
      "BAC library", 
      "defense responses", 
      "susceptible accessions", 
      "rich repeats", 
      "dominant gene", 
      "F1 individuals", 
      "apple breeding", 
      "apple accessions", 
      "untranslated region", 
      "chromosome 11", 
      "genes", 
      "susceptibility/resistance", 
      "loci", 
      "Alternaria alternata", 
      "blotch", 
      "crop production", 
      "alternata", 
      "major diseases", 
      "accessions", 
      "susceptibility of apples", 
      "pathotypes", 
      "plants", 
      "protein", 
      "new insights", 
      "cultivars", 
      "apple cultivars", 
      "breeding", 
      "repeats", 
      "BackgroundThe mechanism", 
      "kb", 
      "apples", 
      "alleles", 
      "susceptibility", 
      "mechanism", 
      "first time", 
      "region", 
      "East Asia", 
      "cross", 
      "Delicious", 
      "Starking Delicious", 
      "toxin", 
      "particular interest", 
      "resistance", 
      "identification", 
      "library", 
      "largest producer", 
      "insights", 
      "insertion", 
      "production", 
      "interaction", 
      "absence", 
      "producers", 
      "response", 
      "determinants", 
      "Asia", 
      "disease control", 
      "presence", 
      "older ones", 
      "individuals", 
      "infection", 
      "disease", 
      "founders", 
      "maps", 
      "ResultsWe", 
      "control", 
      "findings", 
      "study", 
      "interest", 
      "HST", 
      "one", 
      "time", 
      "ALT", 
      "coil", 
      "top", 
      "sports"
    ], 
    "name": "Identification of candidate genes responsible for the susceptibility of apple (Malus \u00d7 domestica Borkh.) to Alternaria blotch", 
    "pagination": "132", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1113300161"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12870-019-1737-7"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30961541"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12870-019-1737-7", 
      "https://app.dimensions.ai/details/publication/pub.1113300161"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-11-24T21:03", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_807.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/s12870-019-1737-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12870-019-1737-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12870-019-1737-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12870-019-1737-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12870-019-1737-7'


 

This table displays all metadata directly associated to this object as RDF triples.

298 TRIPLES      21 PREDICATES      133 URIs      115 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12870-019-1737-7 schema:about N287c5d6a3423454482a6cc7ac2f33d8e
2 N367e8e641f094b8da0fad96e58257fed
3 N5b65c07c31764f3596cd77174b185b0a
4 N691430f3b8434ad4b6c1b3eef2575675
5 N7bee73a0de6e4f3ba42678f039111f65
6 Nbb8e33dd283b47ebb04cd82ec04eb329
7 Nfb67c731507b4563a3d5f0c6bfe06471
8 Nff9f02bb1ca542a8a1e6512fd28d5354
9 anzsrc-for:06
10 anzsrc-for:0604
11 schema:author N0a0bcf9a260a4a5cb6fb9c323f751bc3
12 schema:citation sg:pub.10.1007/s002940100233
13 sg:pub.10.1007/s10327-008-0089-1
14 sg:pub.10.1007/s10327-013-0498-7
15 sg:pub.10.1007/s10658-016-0866-1
16 sg:pub.10.1007/s11295-006-0045-1
17 sg:pub.10.1038/cdd.2011.37
18 sg:pub.10.1038/nature01184
19 sg:pub.10.1038/ng.3886
20 sg:pub.10.1038/ng.654
21 sg:pub.10.1186/1471-2105-9-253
22 schema:datePublished 2019-04-08
23 schema:datePublishedReg 2019-04-08
24 schema:description BackgroundThe mechanism underlying the interaction between host plant and host-selective toxin (HST)-producing Alternaria alternata during infection is of particular interest for sustainable crop production. Alternaria blotch of apple (Malus × domestica Borkh.) caused by A. alternata apple pathotype is a major disease particularly in East Asia, which is the largest producer of apples globally. A single dominant gene, Alt, controls the susceptibility of the apple cultivar ‘Delicious’ to Alternaria blotch. In this study, we fine mapped the Alt locus and characterized three potential candidate genes.ResultsWe used 797 F1 individuals derived from 15 crosses between apple accessions susceptible (Alt/alt) and resistant (alt/alt) to Alternaria blotch to construct physical and genetic maps of the Alt locus located on the top of chromosome 11. Susceptible accessions were derived from ‘Delicious.’ To fine map the Alt locus, we constructed a BAC library of ‘Starking Delicious,’ a sport of ‘Delicious,’ and used graphical genotyping to delimit the Alt locus to a region of 43 kb. Three genes predicted within the candidate Alt region were potentially involved in plant defense response, among which the gene encoding a coiled coil-nucleotide binding-leucine rich repeat (CC-NB-LRR) type disease resistance protein was the most promising. Moreover, a 12-bp insertion was uniquely identified in the 5′ untranslated region of the Alt-associated allele of this gene, the presence or absence of which co-segregated with the susceptibility or resistance to A. alternata apple pathotype, respectively, among 43 tested cultivars including old ones and founders of modern apple breeding.ConclusionA disease resistance protein has been suggested as a determinant of susceptibility/resistance to HST-producing A. alternata for the first time. Our finding provides new insight into the mechanism of HST-mediated disease control used by A. alternata against host plants.
25 schema:genre article
26 schema:isAccessibleForFree true
27 schema:isPartOf N5571d83bcb874a98b900957a1e84bbee
28 Ncc9f0fb63ac84e71990e857eaf6ff2e9
29 sg:journal.1024638
30 schema:keywords A. alternata
31 A. alternata apple pathotype
32 ALT
33 Alt locus
34 Alternaria alternata
35 Alternaria blotch
36 Asia
37 BAC library
38 BackgroundThe mechanism
39 Delicious
40 East Asia
41 F1 individuals
42 HST
43 ResultsWe
44 Starking Delicious
45 absence
46 accessions
47 alleles
48 alternata
49 apple accessions
50 apple breeding
51 apple cultivars
52 apple pathotype
53 apples
54 blotch
55 breeding
56 candidate genes
57 chromosome 11
58 coil
59 control
60 crop production
61 cross
62 cultivars
63 defense responses
64 determinants
65 disease
66 disease control
67 disease resistance proteins
68 dominant gene
69 findings
70 first time
71 founders
72 genes
73 genetic map
74 host plants
75 host-selective toxin
76 identification
77 individuals
78 infection
79 insertion
80 insights
81 interaction
82 interest
83 kb
84 largest producer
85 library
86 loci
87 major diseases
88 maps
89 mechanism
90 new insights
91 older ones
92 one
93 particular interest
94 pathotypes
95 plant defense responses
96 plants
97 potential candidate genes
98 presence
99 producers
100 production
101 protein
102 region
103 repeats
104 resistance
105 resistance protein
106 response
107 rich repeats
108 single dominant gene
109 sports
110 study
111 susceptibility
112 susceptibility of apples
113 susceptibility/resistance
114 susceptible accessions
115 sustainable crop production
116 time
117 top
118 toxin
119 untranslated region
120 schema:name Identification of candidate genes responsible for the susceptibility of apple (Malus × domestica Borkh.) to Alternaria blotch
121 schema:pagination 132
122 schema:productId N4f81781648774d92a849280d79a74655
123 N75ed0517dc4f4390ab2faa2559471dfa
124 Nd61d3c769074434389a2e8845556dbb7
125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1113300161
126 https://doi.org/10.1186/s12870-019-1737-7
127 schema:sdDatePublished 2022-11-24T21:03
128 schema:sdLicense https://scigraph.springernature.com/explorer/license/
129 schema:sdPublisher N610aeec512bd44068750a251e9a66109
130 schema:url https://doi.org/10.1186/s12870-019-1737-7
131 sgo:license sg:explorer/license/
132 sgo:sdDataset articles
133 rdf:type schema:ScholarlyArticle
134 N0a0bcf9a260a4a5cb6fb9c323f751bc3 rdf:first sg:person.01067604123.45
135 rdf:rest N3f6526dfbd5046e1bc57a3f34ec5f686
136 N287c5d6a3423454482a6cc7ac2f33d8e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Malus
138 rdf:type schema:DefinedTerm
139 N367e8e641f094b8da0fad96e58257fed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Disease Resistance
141 rdf:type schema:DefinedTerm
142 N3f6526dfbd5046e1bc57a3f34ec5f686 rdf:first sg:person.015205461257.49
143 rdf:rest N6e8e3f52ba33447d8478cedaf63fc073
144 N4f81781648774d92a849280d79a74655 schema:name dimensions_id
145 schema:value pub.1113300161
146 rdf:type schema:PropertyValue
147 N5571d83bcb874a98b900957a1e84bbee schema:volumeNumber 19
148 rdf:type schema:PublicationVolume
149 N55aadd50e4714a4389fd0b0ad1f33746 rdf:first sg:person.0623525444.60
150 rdf:rest Ncdd368764d8c41e89bfb394133399c0a
151 N594b285b21af4116a4dd43337d20ebb7 rdf:first N99cd7274b22f44b1954e288568c8f1b4
152 rdf:rest Nf4cea66a32384961b531b05a933fb064
153 N5b65c07c31764f3596cd77174b185b0a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Gene Library
155 rdf:type schema:DefinedTerm
156 N610aeec512bd44068750a251e9a66109 schema:name Springer Nature - SN SciGraph project
157 rdf:type schema:Organization
158 N691430f3b8434ad4b6c1b3eef2575675 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Plant Proteins
160 rdf:type schema:DefinedTerm
161 N6e8e3f52ba33447d8478cedaf63fc073 rdf:first sg:person.01021470723.37
162 rdf:rest N9dd5c9ce3f84435c90e2a941ad755a3b
163 N75ed0517dc4f4390ab2faa2559471dfa schema:name doi
164 schema:value 10.1186/s12870-019-1737-7
165 rdf:type schema:PropertyValue
166 N7bee73a0de6e4f3ba42678f039111f65 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Proteins
168 rdf:type schema:DefinedTerm
169 N99cd7274b22f44b1954e288568c8f1b4 schema:affiliation grid-institutes:grid.419573.d
170 schema:familyName Wu
171 schema:givenName Jianzhon
172 rdf:type schema:Person
173 N9aafea249d0645c086b453df791d4260 rdf:first sg:person.0647572154.66
174 rdf:rest Nb77eabe220074e408e1cd0ceaa6a505c
175 N9dd5c9ce3f84435c90e2a941ad755a3b rdf:first sg:person.015235550023.35
176 rdf:rest Nd6731fe253ba4bbaa62497a7c457d509
177 Nb77eabe220074e408e1cd0ceaa6a505c rdf:first sg:person.01110271121.32
178 rdf:rest N594b285b21af4116a4dd43337d20ebb7
179 Nbb8e33dd283b47ebb04cd82ec04eb329 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name Plant Diseases
181 rdf:type schema:DefinedTerm
182 Ncc9f0fb63ac84e71990e857eaf6ff2e9 schema:issueNumber 1
183 rdf:type schema:PublicationIssue
184 Ncdd368764d8c41e89bfb394133399c0a rdf:first sg:person.01204032523.63
185 rdf:rest rdf:nil
186 Nd61d3c769074434389a2e8845556dbb7 schema:name pubmed_id
187 schema:value 30961541
188 rdf:type schema:PropertyValue
189 Nd6731fe253ba4bbaa62497a7c457d509 rdf:first sg:person.0710322373.00
190 rdf:rest N9aafea249d0645c086b453df791d4260
191 Nf4cea66a32384961b531b05a933fb064 rdf:first sg:person.0657614121.08
192 rdf:rest N55aadd50e4714a4389fd0b0ad1f33746
193 Nfb67c731507b4563a3d5f0c6bfe06471 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
194 schema:name Leucine-Rich Repeat Proteins
195 rdf:type schema:DefinedTerm
196 Nff9f02bb1ca542a8a1e6512fd28d5354 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
197 schema:name Alternaria
198 rdf:type schema:DefinedTerm
199 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
200 schema:name Biological Sciences
201 rdf:type schema:DefinedTerm
202 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
203 schema:name Genetics
204 rdf:type schema:DefinedTerm
205 sg:journal.1024638 schema:issn 1471-2229
206 schema:name BMC Plant Biology
207 schema:publisher Springer Nature
208 rdf:type schema:Periodical
209 sg:person.01021470723.37 schema:affiliation grid-institutes:grid.482552.c
210 schema:familyName Okada
211 schema:givenName Kazuma
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021470723.37
213 rdf:type schema:Person
214 sg:person.01067604123.45 schema:affiliation grid-institutes:grid.482552.c
215 schema:familyName Moriya
216 schema:givenName Shigeki
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067604123.45
218 rdf:type schema:Person
219 sg:person.01110271121.32 schema:affiliation grid-institutes:grid.419573.d
220 schema:familyName Fujisawa
221 schema:givenName Hiroko
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110271121.32
223 rdf:type schema:Person
224 sg:person.01204032523.63 schema:affiliation grid-institutes:grid.482552.c
225 schema:familyName Abe
226 schema:givenName Kazuyuki
227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204032523.63
228 rdf:type schema:Person
229 sg:person.015205461257.49 schema:affiliation grid-institutes:grid.482552.c
230 schema:familyName Terakami
231 schema:givenName Shingo
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015205461257.49
233 rdf:type schema:Person
234 sg:person.015235550023.35 schema:affiliation grid-institutes:grid.482552.c
235 schema:familyName Shimizu
236 schema:givenName Taku
237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015235550023.35
238 rdf:type schema:Person
239 sg:person.0623525444.60 schema:affiliation grid-institutes:grid.482552.c
240 schema:familyName Yamamoto
241 schema:givenName Toshiya
242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623525444.60
243 rdf:type schema:Person
244 sg:person.0647572154.66 schema:affiliation grid-institutes:grid.419573.d
245 schema:familyName Katayose
246 schema:givenName Yuichi
247 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647572154.66
248 rdf:type schema:Person
249 sg:person.0657614121.08 schema:affiliation grid-institutes:grid.419573.d
250 schema:familyName Kanamori
251 schema:givenName Hiroyuki
252 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657614121.08
253 rdf:type schema:Person
254 sg:person.0710322373.00 schema:affiliation grid-institutes:grid.482552.c
255 schema:familyName Adachi
256 schema:givenName Yoshihiko
257 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0710322373.00
258 rdf:type schema:Person
259 sg:pub.10.1007/s002940100233 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030269188
260 https://doi.org/10.1007/s002940100233
261 rdf:type schema:CreativeWork
262 sg:pub.10.1007/s10327-008-0089-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014897425
263 https://doi.org/10.1007/s10327-008-0089-1
264 rdf:type schema:CreativeWork
265 sg:pub.10.1007/s10327-013-0498-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040845188
266 https://doi.org/10.1007/s10327-013-0498-7
267 rdf:type schema:CreativeWork
268 sg:pub.10.1007/s10658-016-0866-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025625115
269 https://doi.org/10.1007/s10658-016-0866-1
270 rdf:type schema:CreativeWork
271 sg:pub.10.1007/s11295-006-0045-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010221339
272 https://doi.org/10.1007/s11295-006-0045-1
273 rdf:type schema:CreativeWork
274 sg:pub.10.1038/cdd.2011.37 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030921204
275 https://doi.org/10.1038/cdd.2011.37
276 rdf:type schema:CreativeWork
277 sg:pub.10.1038/nature01184 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046673491
278 https://doi.org/10.1038/nature01184
279 rdf:type schema:CreativeWork
280 sg:pub.10.1038/ng.3886 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085864660
281 https://doi.org/10.1038/ng.3886
282 rdf:type schema:CreativeWork
283 sg:pub.10.1038/ng.654 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025100649
284 https://doi.org/10.1038/ng.654
285 rdf:type schema:CreativeWork
286 sg:pub.10.1186/1471-2105-9-253 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049665949
287 https://doi.org/10.1186/1471-2105-9-253
288 rdf:type schema:CreativeWork
289 grid-institutes:grid.419573.d schema:alternateName Advanced Genomics Breeding Section, Institute of Crop Science, NARO, 1-2 Ohwashi, 305-8634, Tsukuba, Ibaraki, Japan
290 schema:name Advanced Genomics Breeding Section, Institute of Crop Science, NARO, 1-2 Ohwashi, 305-8634, Tsukuba, Ibaraki, Japan
291 rdf:type schema:Organization
292 grid-institutes:grid.482552.c schema:alternateName Apple Research Station, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 92-24 Shimokuriyagawa Nabeyashiki, 020-0123, Morioka, Iwate, Japan
293 Citrus Research Station, Institute of Fruit Tree and Tea Science, NARO, 485-6 Okitsunaka-cho, Shimizu, 424-0284, Shizuoka City, Shizuoka, Japan
294 Institute of Fruit Tree and Tea Science, NARO, 2-1 Fujimoto, 305-8604, Tsukuba, Ibaraki, Japan
295 schema:name Apple Research Station, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 92-24 Shimokuriyagawa Nabeyashiki, 020-0123, Morioka, Iwate, Japan
296 Citrus Research Station, Institute of Fruit Tree and Tea Science, NARO, 485-6 Okitsunaka-cho, Shimizu, 424-0284, Shizuoka City, Shizuoka, Japan
297 Institute of Fruit Tree and Tea Science, NARO, 2-1 Fujimoto, 305-8604, Tsukuba, Ibaraki, Japan
298 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...