Cgl2 plays an essential role in cuticular wax biosynthesis in cabbage (Brassica oleracea L. var. capitata) View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-11-28

AUTHORS

Dongming Liu, Jun Tang, Zezhou Liu, Xin Dong, Mu Zhuang, Yangyong Zhang, Honghao Lv, Peitian Sun, Yumei Liu, Zhansheng Li, Zhibiao Ye, Zhiyuan Fang, Limei Yang

ABSTRACT

BACKGROUND: The aerial parts of most land plants are covered with cuticular wax which is important for plants to avoid harmful factors. There is still no cloning study about wax synthesis gene of the alcohol-forming pathway in Brassica species. RESULTS: Scanning electron microscopy (SEM) showed that, compared with wild type (WT), wax crystal are severely reduced in both the adaxial and abaxial sides of cabbage (Brassica oleracea L. var. capitata L.) leaves from the LD10GL mutant. Genetic analysis results revealed that the glossy trait of LD10GL is controlled by a single recessive gene, and fine mapping results revealed that the target gene Cgl2 (Cabbage glossy 2) is located within a physical region of 170 kb on chromosome 1. Based on sequence analysis of the genes in the mapped region, the gene designated Bol013612 was speculated to be the candidate gene. Gene Bol013612 is homologous to Arabidopsis CER4, which encodes fatty acyl-coenzyme A reductase. Sequencing identified a single nucleotide substitution at an intron/exon boundary that results in an insertion of six nucleotides in the cDNA of Bol013612 in LD10GL. The phenotypic defect of LD10GL was confirmed by a functional complementation test with Arabidopsis mutant cer4. CONCLUSIONS: Our results indicated that wax crystals of cabbage mutant LD10GL are severely reduced and mutation of gene Bol013612 causes a glossy phenotype in the LD10GL mutant. More... »

PAGES

223

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12870-017-1162-8

DOI

http://dx.doi.org/10.1186/s12870-017-1162-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1093037341

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29179675


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21 schema:description BACKGROUND: The aerial parts of most land plants are covered with cuticular wax which is important for plants to avoid harmful factors. There is still no cloning study about wax synthesis gene of the alcohol-forming pathway in Brassica species. RESULTS: Scanning electron microscopy (SEM) showed that, compared with wild type (WT), wax crystal are severely reduced in both the adaxial and abaxial sides of cabbage (Brassica oleracea L. var. capitata L.) leaves from the LD10GL mutant. Genetic analysis results revealed that the glossy trait of LD10GL is controlled by a single recessive gene, and fine mapping results revealed that the target gene Cgl2 (Cabbage glossy 2) is located within a physical region of 170 kb on chromosome 1. Based on sequence analysis of the genes in the mapped region, the gene designated Bol013612 was speculated to be the candidate gene. Gene Bol013612 is homologous to Arabidopsis CER4, which encodes fatty acyl-coenzyme A reductase. Sequencing identified a single nucleotide substitution at an intron/exon boundary that results in an insertion of six nucleotides in the cDNA of Bol013612 in LD10GL. The phenotypic defect of LD10GL was confirmed by a functional complementation test with Arabidopsis mutant cer4. CONCLUSIONS: Our results indicated that wax crystals of cabbage mutant LD10GL are severely reduced and mutation of gene Bol013612 causes a glossy phenotype in the LD10GL mutant.
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29 Arabidopsis mutant cer4
30 Bol013612
31 Brassica species
32 CER4
33 CGL2
34 Gene Bol013612
35 LD10GL
36 LD10GL mutant
37 abaxial side
38 aerial parts
39 alcohol-forming pathway
40 analysis
41 analysis results
42 biosynthesis
43 boundaries
44 cDNA
45 cDNA of Bol013612
46 cabbage
47 cabbage mutant LD10GL
48 candidate genes
49 chromosome 1
50 cloning studies
51 complementation tests
52 crystals
53 cuticular wax biosynthesis
54 cuticular waxes
55 defects
56 electron microscopy
57 essential role
58 exon boundaries
59 factors
60 fine mapping results
61 functional complementation tests
62 gene Cgl2
63 genes
64 genetic analysis results
65 glossy phenotype
66 glossy trait
67 harmful factors
68 insertion
69 intron/exon boundaries
70 kb
71 land plants
72 mapping results
73 microscopy
74 most land plants
75 mutant LD10GL
76 mutant cer4
77 mutants
78 mutations
79 nucleotide substitutions
80 nucleotides
81 part
82 pathway
83 phenotype
84 phenotypic defects
85 physical region
86 plants
87 recessive gene
88 reductase
89 region
90 results
91 role
92 sequence analysis
93 sequencing
94 side
95 single nucleotide substitution
96 single recessive gene
97 species
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100 synthesis genes
101 target gene Cgl2
102 test
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