Identification and use of the sugarcane bacilliform virus enhancer in transgenic maize View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-12-19

AUTHORS

John P Davies, Vaka Reddy, Xing L Liu, Avutu S Reddy, William Michael Ainley, Mark Thompson, Lakshmi Sastry-Dent, Zehui Cao, James Connell, Delkin O Gonzalez, Douglas Ry Wagner

ABSTRACT

BackgroundTranscriptional enhancers are able to increase transcription from heterologous promoters when placed upstream, downstream and in either orientation, relative to the promoter. Transcriptional enhancers have been used to enhance expression of specific promoters in transgenic plants and in activation tagging studies to help elucidate gene function.ResultsA transcriptional enhancer from the Sugarcane Bacilliform Virus - Ireng Maleng isolate (SCBV-IM) that can cause increased transcription when integrated into the the genome near maize genes has been identified. In transgenic maize, the SCBV-IM promoter was shown to be comparable in strength to the maize ubiquitin 1 promoter in young leaf and root tissues. The promoter was dissected to identify sequences that confer high activity in transient assays. Enhancer sequences were identified and shown to increase the activity of a heterologous truncated promoter. These enhancer sequences were shown to be more active when arrayed in 4 copy arrays than in 1 or 2 copy arrays. When the enhancer array was transformed into maize plants it caused an increase in accumulation of transcripts of genes near the site of integration in the genome.ConclusionsThe SCBV-IM enhancer can activate transcription upstream or downstream of genes and in either orientation. It may be a useful tool to activate enhance from specific promoters or in activation tagging. More... »

PAGES

359

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12870-014-0359-3

    DOI

    http://dx.doi.org/10.1186/s12870-014-0359-3

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1043533395

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/25526789


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    330 Dow AgroSciences, 9330 Zionsville Rd, 46268, Indianapolis, IN, USA
    331 schema:name Dow AgroSciences, 16160 SW Upper Boones Ferry Rd, 97224, Portland, OR, USA
    332 Dow AgroSciences, 9330 Zionsville Rd, 46268, Indianapolis, IN, USA
    333 rdf:type schema:Organization
     




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