Mechanisms of linezolid resistance in Staphylococcus capitis with the novel mutation C2128T in the 23S rRNA gene in China View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2022-08-20

AUTHORS

Xiao Han, Guiling Zou, Jiaren Liu, Chun Yang, Xuefei Du, Guoyu Chen, Zhe Sun, Xinyu Zhang, Yu Sun, Wanying Zhang, Xiaofeng Jiang

ABSTRACT

PurposeThe objective of this study was to investigate the molecular characteristics and potential resistance mechanisms of linezolid-resistant (LZR) Staphylococcus capitis isolates from a tertiary hospital in China.MethodsS. capitis isolates were obtained from clinical patient specimens; three of the isolates came from blood cultures and one from the hydrothorax. The agar dilution and E-test methods were used to identify antibiotic resistance. The chloramphenicol-florfenicol resistance (cfr) gene carrier status of the strains was determined by PCR. Whole-genome sequencing (WGS) was used to identify point mutations and L3, L4, and L22 mutations and to study the genetic environment of the cfr gene and the relationships between strains.ResultsThe 4 isolates obtained in this study were all linezolid-resistant Staphylococcus strains. A similar of susceptibility profile pattern was observed in all four S. capitis strains, each of which exhibited a multidrug-resistant phenotype. A potentially novel mutation, C2128T, was identified, and the cfr genes of S. capitis strains were all positive. Additionally, the same mutations (C2128T and G2600T) were identified in all 23S rRNA sequences of the isolates, whereas mutations were lacking in the L3, L4, and L22 ribosomal proteins. The genetic environments surrounding cfr were identical in all four isolates. A schematic diagram of the phylogenetic tree showed that they were closely related to AYP1020, CR01, and TW2795, and a total of seven drug resistance genes were identified in these strains.ConclusionsThe study indicated that the resistance of the Staphylococcus capitis strains to linezolid was caused by multiple mechanisms, and a potential novel mutation, C2128T, that may have an impact on bacterial resistance was identified. More... »

PAGES

203

References to SciGraph publications

  • 2020-09-24. Staphylococcus capitis causing infective endocarditis: not so uncommon in INFECTION
  • 2018-06-07. Coagulase-Negative Staphylococcus Skin and Soft Tissue Infections in AMERICAN JOURNAL OF CLINICAL DERMATOLOGY
  • 2020-10-26. Prevalence and mechanisms of linezolid resistance among staphylococcal clinical isolates from Egypt in EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12866-022-02616-9

    DOI

    http://dx.doi.org/10.1186/s12866-022-02616-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1150382233

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/35987607


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Anti-Bacterial Agents", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Drug Resistance, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, rRNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Linezolid", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Methicillin-Resistant Staphylococcus aureus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Microbial Sensitivity Tests", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mutation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phylogeny", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA, Ribosomal, 23S", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Staphylococcal Infections", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Staphylococcus capitis", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China", 
              "id": "http://www.grid.ac/institutes/grid.411491.8", 
              "name": [
                "The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Han", 
            "givenName": "Xiao", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China", 
                "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zou", 
            "givenName": "Guiling", 
            "id": "sg:person.011771257121.72", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011771257121.72"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China", 
                "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Liu", 
            "givenName": "Jiaren", 
            "id": "sg:person.07645570061.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07645570061.29"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China", 
                "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "Chun", 
            "id": "sg:person.014534342743.96", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014534342743.96"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China", 
                "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Du", 
            "givenName": "Xuefei", 
            "id": "sg:person.01211376030.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211376030.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China", 
                "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chen", 
            "givenName": "Guoyu", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China", 
                "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sun", 
            "givenName": "Zhe", 
            "id": "sg:person.014161600521.49", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014161600521.49"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China", 
                "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Xinyu", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China", 
                "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sun", 
            "givenName": "Yu", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China", 
                "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Wanying", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China", 
                "Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jiang", 
            "givenName": "Xiaofeng", 
            "id": "sg:person.01361247374.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361247374.22"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/s15010-020-01514-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1131165152", 
              "https://doi.org/10.1007/s15010-020-01514-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s40257-018-0362-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1104468493", 
              "https://doi.org/10.1007/s40257-018-0362-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10096-020-04045-w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1132048327", 
              "https://doi.org/10.1007/s10096-020-04045-w"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2022-08-20", 
        "datePublishedReg": "2022-08-20", 
        "description": "PurposeThe objective of this study was to investigate the molecular characteristics and potential resistance mechanisms of linezolid-resistant (LZR) Staphylococcus capitis isolates from a tertiary hospital in China.MethodsS. capitis isolates were obtained from clinical patient specimens; three of the isolates came from blood cultures and one from the hydrothorax. The agar dilution and E-test methods were used to identify antibiotic resistance. The chloramphenicol-florfenicol resistance (cfr) gene carrier status of the strains was determined by PCR. Whole-genome sequencing (WGS) was used to identify point mutations and L3, L4, and L22 mutations and to study the genetic environment of the cfr gene and the relationships between strains.ResultsThe 4 isolates obtained in this study were all linezolid-resistant Staphylococcus strains. A similar of susceptibility profile pattern was observed in all four S. capitis strains, each of which exhibited a multidrug-resistant phenotype. A potentially novel mutation, C2128T, was identified, and the cfr genes of S. capitis strains were all positive. Additionally, the same mutations (C2128T and G2600T) were identified in all 23S rRNA sequences of the isolates, whereas mutations were lacking in the L3, L4, and L22 ribosomal proteins. The genetic environments surrounding cfr were identical in all four isolates. A schematic diagram of the phylogenetic tree showed that they were closely related to AYP1020, CR01, and TW2795, and a total of seven drug resistance genes were identified in these strains.ConclusionsThe study indicated that the resistance of the Staphylococcus capitis strains to linezolid was caused by multiple mechanisms, and a potential novel mutation, C2128T, that may have an impact on bacterial resistance was identified.", 
        "genre": "article", 
        "id": "sg:pub.10.1186/s12866-022-02616-9", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.6979497", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1024253", 
            "issn": [
              "1471-2180"
            ], 
            "name": "BMC Microbiology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "22"
          }
        ], 
        "keywords": [
          "whole-genome sequencing", 
          "potential novel mutations", 
          "genetic environment", 
          "L22 ribosomal proteins", 
          "novel mutations", 
          "ribosomal proteins", 
          "phylogenetic tree", 
          "drug resistance genes", 
          "rRNA gene", 
          "Staphylococcus capitis", 
          "rRNA sequences", 
          "potential resistance mechanisms", 
          "clinical patient specimens", 
          "resistance genes", 
          "genes", 
          "point mutations", 
          "L22 mutations", 
          "mutations", 
          "resistance mechanisms", 
          "S. capitis", 
          "same mutation", 
          "multiple mechanisms", 
          "molecular characteristics", 
          "multidrug-resistant phenotype", 
          "isolates", 
          "bacterial resistance", 
          "antibiotic resistance", 
          "cfr gene", 
          "strains", 
          "linezolid-resistant staphylococci", 
          "mechanism", 
          "sequencing", 
          "protein", 
          "phenotype", 
          "CR01", 
          "patient specimens", 
          "resistance", 
          "sequence", 
          "trees", 
          "PCR", 
          "gene carrier status", 
          "profile patterns", 
          "L3", 
          "environment", 
          "carrier status", 
          "culture", 
          "L4", 
          "patterns", 
          "study", 
          "Similar", 
          "staphylococci", 
          "linezolid resistance", 
          "China", 
          "specimens", 
          "relationship", 
          "ConclusionsThe study", 
          "status", 
          "CFR", 
          "capitis", 
          "dilution", 
          "total", 
          "impact", 
          "characteristics", 
          "schematic diagram", 
          "objective", 
          "agar dilution", 
          "method", 
          "E-test method", 
          "blood cultures", 
          "linezolid", 
          "tertiary hospital", 
          "hydrothorax", 
          "diagram", 
          "hospital"
        ], 
        "name": "Mechanisms of linezolid resistance in Staphylococcus capitis with the novel mutation C2128T in the 23S rRNA gene in China", 
        "pagination": "203", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1150382233"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s12866-022-02616-9"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "35987607"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s12866-022-02616-9", 
          "https://app.dimensions.ai/details/publication/pub.1150382233"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-12-01T06:44", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_942.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1186/s12866-022-02616-9"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12866-022-02616-9'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12866-022-02616-9'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12866-022-02616-9'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12866-022-02616-9'


     

    This table displays all metadata directly associated to this object as RDF triples.

    265 TRIPLES      21 PREDICATES      114 URIs      103 LITERALS      19 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s12866-022-02616-9 schema:about N15c24d3a81bd4a33807bd070da042e3d
    2 N35f3ca5b99d5469eab93423944d3a0a7
    3 N3b306464a25544319a81fc3996efa4ea
    4 N5530ca0dd8d74dca844a41f7f80625e3
    5 N5ee1b2befb24468eac005607c94750bb
    6 N631e0b0961d847baa0ffc625b8d7e733
    7 Nb0dccdfb518845f28bdfca4a4059023f
    8 Nb8eb34b0676249b3af93bd3b27d14c53
    9 Nbc93b2aa85d948e58146ccf9c80a6b00
    10 Necf14fc227f34f73af93f465324f20cb
    11 Nf15512b8d88347c7997a9c35b78072c0
    12 Nfd9bc60785e74eff8765c5f4c2c50229
    13 anzsrc-for:06
    14 anzsrc-for:0604
    15 schema:author N5e8fd896fbf24072b19e1f697c8c7b71
    16 schema:citation sg:pub.10.1007/s10096-020-04045-w
    17 sg:pub.10.1007/s15010-020-01514-4
    18 sg:pub.10.1007/s40257-018-0362-9
    19 schema:datePublished 2022-08-20
    20 schema:datePublishedReg 2022-08-20
    21 schema:description PurposeThe objective of this study was to investigate the molecular characteristics and potential resistance mechanisms of linezolid-resistant (LZR) Staphylococcus capitis isolates from a tertiary hospital in China.MethodsS. capitis isolates were obtained from clinical patient specimens; three of the isolates came from blood cultures and one from the hydrothorax. The agar dilution and E-test methods were used to identify antibiotic resistance. The chloramphenicol-florfenicol resistance (cfr) gene carrier status of the strains was determined by PCR. Whole-genome sequencing (WGS) was used to identify point mutations and L3, L4, and L22 mutations and to study the genetic environment of the cfr gene and the relationships between strains.ResultsThe 4 isolates obtained in this study were all linezolid-resistant Staphylococcus strains. A similar of susceptibility profile pattern was observed in all four S. capitis strains, each of which exhibited a multidrug-resistant phenotype. A potentially novel mutation, C2128T, was identified, and the cfr genes of S. capitis strains were all positive. Additionally, the same mutations (C2128T and G2600T) were identified in all 23S rRNA sequences of the isolates, whereas mutations were lacking in the L3, L4, and L22 ribosomal proteins. The genetic environments surrounding cfr were identical in all four isolates. A schematic diagram of the phylogenetic tree showed that they were closely related to AYP1020, CR01, and TW2795, and a total of seven drug resistance genes were identified in these strains.ConclusionsThe study indicated that the resistance of the Staphylococcus capitis strains to linezolid was caused by multiple mechanisms, and a potential novel mutation, C2128T, that may have an impact on bacterial resistance was identified.
    22 schema:genre article
    23 schema:isAccessibleForFree true
    24 schema:isPartOf N9221c3911e814affb306934b733f6fce
    25 Nc18f9f5de5ac42ac96e2a5ba280f8b79
    26 sg:journal.1024253
    27 schema:keywords CFR
    28 CR01
    29 China
    30 ConclusionsThe study
    31 E-test method
    32 L22 mutations
    33 L22 ribosomal proteins
    34 L3
    35 L4
    36 PCR
    37 S. capitis
    38 Similar
    39 Staphylococcus capitis
    40 agar dilution
    41 antibiotic resistance
    42 bacterial resistance
    43 blood cultures
    44 capitis
    45 carrier status
    46 cfr gene
    47 characteristics
    48 clinical patient specimens
    49 culture
    50 diagram
    51 dilution
    52 drug resistance genes
    53 environment
    54 gene carrier status
    55 genes
    56 genetic environment
    57 hospital
    58 hydrothorax
    59 impact
    60 isolates
    61 linezolid
    62 linezolid resistance
    63 linezolid-resistant staphylococci
    64 mechanism
    65 method
    66 molecular characteristics
    67 multidrug-resistant phenotype
    68 multiple mechanisms
    69 mutations
    70 novel mutations
    71 objective
    72 patient specimens
    73 patterns
    74 phenotype
    75 phylogenetic tree
    76 point mutations
    77 potential novel mutations
    78 potential resistance mechanisms
    79 profile patterns
    80 protein
    81 rRNA gene
    82 rRNA sequences
    83 relationship
    84 resistance
    85 resistance genes
    86 resistance mechanisms
    87 ribosomal proteins
    88 same mutation
    89 schematic diagram
    90 sequence
    91 sequencing
    92 specimens
    93 staphylococci
    94 status
    95 strains
    96 study
    97 tertiary hospital
    98 total
    99 trees
    100 whole-genome sequencing
    101 schema:name Mechanisms of linezolid resistance in Staphylococcus capitis with the novel mutation C2128T in the 23S rRNA gene in China
    102 schema:pagination 203
    103 schema:productId N69c4d27b22a14cafa5eb67fd9bf2a513
    104 N944533f232184595903d623a8b2b8518
    105 Ne84228d354384effaa5a269e294f718e
    106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1150382233
    107 https://doi.org/10.1186/s12866-022-02616-9
    108 schema:sdDatePublished 2022-12-01T06:44
    109 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    110 schema:sdPublisher Nab12dd2a677c4e5f81fbfa5cc2f87711
    111 schema:url https://doi.org/10.1186/s12866-022-02616-9
    112 sgo:license sg:explorer/license/
    113 sgo:sdDataset articles
    114 rdf:type schema:ScholarlyArticle
    115 N14241599a0874866b5971280467a76ad schema:affiliation grid-institutes:None
    116 schema:familyName Sun
    117 schema:givenName Yu
    118 rdf:type schema:Person
    119 N15c24d3a81bd4a33807bd070da042e3d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Anti-Bacterial Agents
    121 rdf:type schema:DefinedTerm
    122 N22d9e01f9e35456988cf80a37585cf7a schema:affiliation grid-institutes:None
    123 schema:familyName Zhang
    124 schema:givenName Wanying
    125 rdf:type schema:Person
    126 N2ff8a4af1e224e68bd9368cf9472f6f4 schema:affiliation grid-institutes:grid.411491.8
    127 schema:familyName Han
    128 schema:givenName Xiao
    129 rdf:type schema:Person
    130 N32929ac902e648a48cd5f2d10cc841ac rdf:first sg:person.07645570061.29
    131 rdf:rest N3ac64a3f7833412daa316bb40719a965
    132 N34b87f0390274f638efca41414e0fd1e schema:affiliation grid-institutes:None
    133 schema:familyName Zhang
    134 schema:givenName Xinyu
    135 rdf:type schema:Person
    136 N35f3ca5b99d5469eab93423944d3a0a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Humans
    138 rdf:type schema:DefinedTerm
    139 N36db66e796ae489db4183a530ef297f9 rdf:first sg:person.01361247374.22
    140 rdf:rest rdf:nil
    141 N3ac64a3f7833412daa316bb40719a965 rdf:first sg:person.014534342743.96
    142 rdf:rest Ne3bfe7c02a59470c951994ea8cc7c246
    143 N3b306464a25544319a81fc3996efa4ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Mutation
    145 rdf:type schema:DefinedTerm
    146 N422f67ffe12842dfbd94ef5a85c5aa4a rdf:first N14241599a0874866b5971280467a76ad
    147 rdf:rest Nf0e6500c9b7d4216970abd4c52bf3f4a
    148 N4eec86f462b84728965aecde7eba131f rdf:first Nf7afca442a0a45fba04591ed40750e5c
    149 rdf:rest Nc584318161f14acca45f45c61d60e6a7
    150 N5530ca0dd8d74dca844a41f7f80625e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    151 schema:name Phylogeny
    152 rdf:type schema:DefinedTerm
    153 N5e8fd896fbf24072b19e1f697c8c7b71 rdf:first N2ff8a4af1e224e68bd9368cf9472f6f4
    154 rdf:rest N60399af55db34b4a8e5e41a207c1a9da
    155 N5ee1b2befb24468eac005607c94750bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Methicillin-Resistant Staphylococcus aureus
    157 rdf:type schema:DefinedTerm
    158 N60399af55db34b4a8e5e41a207c1a9da rdf:first sg:person.011771257121.72
    159 rdf:rest N32929ac902e648a48cd5f2d10cc841ac
    160 N631e0b0961d847baa0ffc625b8d7e733 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name RNA, Ribosomal, 23S
    162 rdf:type schema:DefinedTerm
    163 N69c4d27b22a14cafa5eb67fd9bf2a513 schema:name pubmed_id
    164 schema:value 35987607
    165 rdf:type schema:PropertyValue
    166 N8fb17548aa7e4c88bd1534e50ec5e43e rdf:first N34b87f0390274f638efca41414e0fd1e
    167 rdf:rest N422f67ffe12842dfbd94ef5a85c5aa4a
    168 N9221c3911e814affb306934b733f6fce schema:issueNumber 1
    169 rdf:type schema:PublicationIssue
    170 N944533f232184595903d623a8b2b8518 schema:name dimensions_id
    171 schema:value pub.1150382233
    172 rdf:type schema:PropertyValue
    173 Nab12dd2a677c4e5f81fbfa5cc2f87711 schema:name Springer Nature - SN SciGraph project
    174 rdf:type schema:Organization
    175 Nb0dccdfb518845f28bdfca4a4059023f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    176 schema:name Staphylococcus capitis
    177 rdf:type schema:DefinedTerm
    178 Nb8eb34b0676249b3af93bd3b27d14c53 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    179 schema:name Genes, rRNA
    180 rdf:type schema:DefinedTerm
    181 Nbc93b2aa85d948e58146ccf9c80a6b00 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    182 schema:name Staphylococcal Infections
    183 rdf:type schema:DefinedTerm
    184 Nc18f9f5de5ac42ac96e2a5ba280f8b79 schema:volumeNumber 22
    185 rdf:type schema:PublicationVolume
    186 Nc584318161f14acca45f45c61d60e6a7 rdf:first sg:person.014161600521.49
    187 rdf:rest N8fb17548aa7e4c88bd1534e50ec5e43e
    188 Ne3bfe7c02a59470c951994ea8cc7c246 rdf:first sg:person.01211376030.23
    189 rdf:rest N4eec86f462b84728965aecde7eba131f
    190 Ne84228d354384effaa5a269e294f718e schema:name doi
    191 schema:value 10.1186/s12866-022-02616-9
    192 rdf:type schema:PropertyValue
    193 Necf14fc227f34f73af93f465324f20cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    194 schema:name Drug Resistance, Bacterial
    195 rdf:type schema:DefinedTerm
    196 Nf0e6500c9b7d4216970abd4c52bf3f4a rdf:first N22d9e01f9e35456988cf80a37585cf7a
    197 rdf:rest N36db66e796ae489db4183a530ef297f9
    198 Nf15512b8d88347c7997a9c35b78072c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    199 schema:name Microbial Sensitivity Tests
    200 rdf:type schema:DefinedTerm
    201 Nf7afca442a0a45fba04591ed40750e5c schema:affiliation grid-institutes:None
    202 schema:familyName Chen
    203 schema:givenName Guoyu
    204 rdf:type schema:Person
    205 Nfd9bc60785e74eff8765c5f4c2c50229 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    206 schema:name Linezolid
    207 rdf:type schema:DefinedTerm
    208 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    209 schema:name Biological Sciences
    210 rdf:type schema:DefinedTerm
    211 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    212 schema:name Genetics
    213 rdf:type schema:DefinedTerm
    214 sg:grant.6979497 http://pending.schema.org/fundedItem sg:pub.10.1186/s12866-022-02616-9
    215 rdf:type schema:MonetaryGrant
    216 sg:journal.1024253 schema:issn 1471-2180
    217 schema:name BMC Microbiology
    218 schema:publisher Springer Nature
    219 rdf:type schema:Periodical
    220 sg:person.011771257121.72 schema:affiliation grid-institutes:None
    221 schema:familyName Zou
    222 schema:givenName Guiling
    223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011771257121.72
    224 rdf:type schema:Person
    225 sg:person.01211376030.23 schema:affiliation grid-institutes:None
    226 schema:familyName Du
    227 schema:givenName Xuefei
    228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211376030.23
    229 rdf:type schema:Person
    230 sg:person.01361247374.22 schema:affiliation grid-institutes:None
    231 schema:familyName Jiang
    232 schema:givenName Xiaofeng
    233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361247374.22
    234 rdf:type schema:Person
    235 sg:person.014161600521.49 schema:affiliation grid-institutes:None
    236 schema:familyName Sun
    237 schema:givenName Zhe
    238 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014161600521.49
    239 rdf:type schema:Person
    240 sg:person.014534342743.96 schema:affiliation grid-institutes:None
    241 schema:familyName Yang
    242 schema:givenName Chun
    243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014534342743.96
    244 rdf:type schema:Person
    245 sg:person.07645570061.29 schema:affiliation grid-institutes:None
    246 schema:familyName Liu
    247 schema:givenName Jiaren
    248 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07645570061.29
    249 rdf:type schema:Person
    250 sg:pub.10.1007/s10096-020-04045-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1132048327
    251 https://doi.org/10.1007/s10096-020-04045-w
    252 rdf:type schema:CreativeWork
    253 sg:pub.10.1007/s15010-020-01514-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1131165152
    254 https://doi.org/10.1007/s15010-020-01514-4
    255 rdf:type schema:CreativeWork
    256 sg:pub.10.1007/s40257-018-0362-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104468493
    257 https://doi.org/10.1007/s40257-018-0362-9
    258 rdf:type schema:CreativeWork
    259 grid-institutes:None schema:alternateName Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China
    260 schema:name Heilongjiang Longwei Precision Medical Laboratory Center, Harbin, China
    261 The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China
    262 rdf:type schema:Organization
    263 grid-institutes:grid.411491.8 schema:alternateName The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China
    264 schema:name The Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street, Nangang District, 150001, Harbin, China
    265 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...