Dissemination of IncF-type plasmids in multiresistant CTX-M-15-producing Enterobacteriaceae isolates from surgical-site infections in Bangui, Central African Republic View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-12

AUTHORS

Clotaire Rafaï, Thierry Frank, Alexandre Manirakiza, Alfred Gaudeuille, Jean-Robert Mbecko, Luc Nghario, Eugene Serdouma, Bertrand Tekpa, Benoit Garin, Sebastien Breurec

ABSTRACT

BACKGROUND: Surgical-site infection is the most frequent health care-associated infection in the developing world, with a strikingly higher prevalence than in developed countries We studied the prevalence of resistance to antibiotics in Enterobacteriaceae isolates from surgical-site infections collected in three major tertiary care centres in Bangui, Central African Republic. We also studied the genetic basis for antibiotic resistance and the genetic background of third-generation cephalosporin-resistant (3GC-R) Enterobacteriaceae. RESULTS: Between April 2011 and April 2012, 195 patients with nosocomial surgical-site infections were consecutively recruited into the study at five surgical departments in three major tertiary care centres. Of the 165 bacterial isolates collected, most were Enterobacteriaceae (102/165, 61.8%). Of these, 65/102 (63.7%) were 3GC-R, which were characterized for resistance gene determinants and genetic background. The bla CTX-M-15 and aac(6')-Ib-cr genes were detected in all strains, usually associated with qnr genes (98.5%). Escherichia coli, the most commonly recovered species (33/65, 50.8%), occurred in six different sequence types, including the pandemic B2-O25b-ST131 group (12/33, 36.4%). Resistance transfer was studied in one representative strain of the resistance gene content in each repetitive extragenic palindromic and enterobacterial repetitive intergenic consensus sequence-PCR banding pattern. Plasmids were characterized by PCR-based replicon typing and sub-typing schemes. In most isolates (18/27, 66.7%), bla CTX-M-15 genes were found in incompatibility groups F/F31:A4:B1 and F/F36:A4:B1 conjugative plasmids. Horizontal transfer of both plasmids is probably an important mechanism for the spread of bla CTX-M-15 among Enterobacteriaceae species and hospitals. The presence of sets of antibiotic resistance genes in these two plasmids indicates their capacity for gene rearrangement and their evolution into new variants. CONCLUSIONS: Diverse modes are involved in transmission of resistance, plasmid dissemination probably playing a major role. More... »

PAGES

15

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12866-015-0348-1

DOI

http://dx.doi.org/10.1186/s12866-015-0348-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014284925

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25648151


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Central African Republic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enterobacteriaceae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enterobacteriaceae Infections", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Transfer, Horizontal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Surgical Wound Infection", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tertiary Care Centers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "beta-Lactamases", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institut Pasteur de Bangui", 
          "id": "https://www.grid.ac/institutes/grid.418512.b", 
          "name": [
            "Institut Pasteur, Laboratory of Bacteriology, Avenue de l\u2019Independance, BP 923, Bangui, Central African Republic"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rafa\u00ef", 
        "givenName": "Clotaire", 
        "id": "sg:person.01205573056.81", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205573056.81"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur de Bangui", 
          "id": "https://www.grid.ac/institutes/grid.418512.b", 
          "name": [
            "Institut Pasteur, Laboratory of Bacteriology, Avenue de l\u2019Independance, BP 923, Bangui, Central African Republic"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Frank", 
        "givenName": "Thierry", 
        "id": "sg:person.01310336517.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01310336517.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur de Bangui", 
          "id": "https://www.grid.ac/institutes/grid.418512.b", 
          "name": [
            "Institut Pasteur, Unit of Epidemiology, Avenue de l\u2019Independance, BP 923, Bangui, Central African Republic"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Manirakiza", 
        "givenName": "Alexandre", 
        "id": "sg:person.0641720260.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641720260.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Complexe P\u00e9diatrique, Department of Paediatric Surgery, Avenue de l\u2019Independance, Bangui, Central African Republic"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gaudeuille", 
        "givenName": "Alfred", 
        "id": "sg:person.01306326377.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306326377.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur de Bangui", 
          "id": "https://www.grid.ac/institutes/grid.418512.b", 
          "name": [
            "Institut Pasteur, Laboratory of Bacteriology, Avenue de l\u2019Independance, BP 923, Bangui, Central African Republic"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mbecko", 
        "givenName": "Jean-Robert", 
        "id": "sg:person.01046120114.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046120114.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "H\u00f4pital de l\u2019Amiti\u00e9, Department of General Surgery, Avenue de l\u2019Independance, Bangui, Central African Republic"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nghario", 
        "givenName": "Luc", 
        "id": "sg:person.01126025365.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126025365.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "H\u00f4pital de l\u2019Amiti\u00e9, Department of Gynaecology and Obstetrics, Avenue de l\u2019Independance, Bangui, Central African Republic"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Serdouma", 
        "givenName": "Eugene", 
        "id": "sg:person.01153764400.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153764400.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "H\u00f4pital Communautaire, Department of Orthopaedic Surgery, Avenue des Martyrs, Bangui, Central African Republic"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tekpa", 
        "givenName": "Bertrand", 
        "id": "sg:person.013017672225.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013017672225.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur de Madagascar", 
          "id": "https://www.grid.ac/institutes/grid.418511.8", 
          "name": [
            "Institut Pasteur, Laboratory of Bacteriology, BP 1274, Antananarivo, Madagascar"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Garin", 
        "givenName": "Benoit", 
        "id": "sg:person.016403731464.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016403731464.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur de Bangui", 
          "id": "https://www.grid.ac/institutes/grid.418512.b", 
          "name": [
            "Institut Pasteur, Laboratory of Bacteriology, Avenue de l\u2019Independance, BP 923, Bangui, Central African Republic", 
            "University of Antilles, Faculty of Medecine, Pointe-\u00e0-Pitre, French West Indies"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Breurec", 
        "givenName": "Sebastien", 
        "id": "sg:person.01276276114.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276276114.28"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.ijmm.2011.09.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000264700"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10096-011-1471-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002586959", 
          "https://doi.org/10.1007/s10096-011-1471-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10096-011-1471-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002586959", 
          "https://doi.org/10.1007/s10096-011-1471-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmsa0810119", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003155860"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.48.4.1249-1255.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005036346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dkq415", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007252053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dkq415", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007252053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mimet.2005.03.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009250078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/cmr.14.4.933-951.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013993524"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0463.2007.apm_547.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014282887"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1402.070350", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014602679"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.diagmicrobio.2010.11.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025033090"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dkp194", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026796252"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dkp194", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026796252"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/501620", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027071356"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0147-619x(02)00107-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027921584"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0147-619x(02)00107-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027921584"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/1469-0691.12339", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029828675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ijantimicag.2012.07.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029961817"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dkl309", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032878174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(10)61458-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035917977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.02824-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038451119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1469-0691.2012.03805.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040630788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0005958", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042501168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0195-6701(97)90163-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043914218"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dkq347", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048093799"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1469-0691.2011.03730.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049566366"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/cmr.00016-09", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053076235"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/mdr.2010.0132", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059297764"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2471/blt.11.088179", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070837861"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1701.100244", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071123111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074364313", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2015-12", 
    "datePublishedReg": "2015-12-01", 
    "description": "BACKGROUND: Surgical-site infection is the most frequent health care-associated infection in the developing world, with a strikingly higher prevalence than in developed countries We studied the prevalence of resistance to antibiotics in Enterobacteriaceae isolates from surgical-site infections collected in three major tertiary care centres in Bangui, Central African Republic. We also studied the genetic basis for antibiotic resistance and the genetic background of third-generation cephalosporin-resistant (3GC-R) Enterobacteriaceae.\nRESULTS: Between April 2011 and April 2012, 195 patients with nosocomial surgical-site infections were consecutively recruited into the study at five surgical departments in three major tertiary care centres. Of the 165 bacterial isolates collected, most were Enterobacteriaceae (102/165, 61.8%). Of these, 65/102 (63.7%) were 3GC-R, which were characterized for resistance gene determinants and genetic background. The bla CTX-M-15 and aac(6')-Ib-cr genes were detected in all strains, usually associated with qnr genes (98.5%). Escherichia coli, the most commonly recovered species (33/65, 50.8%), occurred in six different sequence types, including the pandemic B2-O25b-ST131 group (12/33, 36.4%). Resistance transfer was studied in one representative strain of the resistance gene content in each repetitive extragenic palindromic and enterobacterial repetitive intergenic consensus sequence-PCR banding pattern. Plasmids were characterized by PCR-based replicon typing and sub-typing schemes. In most isolates (18/27, 66.7%), bla CTX-M-15 genes were found in incompatibility groups F/F31:A4:B1 and F/F36:A4:B1 conjugative plasmids. Horizontal transfer of both plasmids is probably an important mechanism for the spread of bla CTX-M-15 among Enterobacteriaceae species and hospitals. The presence of sets of antibiotic resistance genes in these two plasmids indicates their capacity for gene rearrangement and their evolution into new variants.\nCONCLUSIONS: Diverse modes are involved in transmission of resistance, plasmid dissemination probably playing a major role.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s12866-015-0348-1", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1024253", 
        "issn": [
          "1471-2180"
        ], 
        "name": "BMC Microbiology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "name": "Dissemination of IncF-type plasmids in multiresistant CTX-M-15-producing Enterobacteriaceae isolates from surgical-site infections in Bangui, Central African Republic", 
    "pagination": "15", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "440d652b0d6f9a4b6d65f9546b53249cdb30ff02d9dbe863004d2482415b7694"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25648151"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100966981"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12866-015-0348-1"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1014284925"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12866-015-0348-1", 
      "https://app.dimensions.ai/details/publication/pub.1014284925"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:57", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89807_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2Fs12866-015-0348-1"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12866-015-0348-1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12866-015-0348-1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12866-015-0348-1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12866-015-0348-1'


 

This table displays all metadata directly associated to this object as RDF triples.

276 TRIPLES      21 PREDICATES      69 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12866-015-0348-1 schema:about N104981897f6d46e2880f0479b3389e13
2 N1c4c4e061b8742838e295391f31ae911
3 N3538fcb799174f38a78d31d987042686
4 N3bd2c07d945f4aebb21177dee713b63a
5 N4273a4287a234b10a5231d4d9e4b9aa1
6 N542b7cb422c4400e96616b90cd1e09ab
7 N95e085a63d214874b750cf3f10b38d1a
8 Na347fbebecb24c858e37f7931adc786a
9 Nac4e531cdde84725b9251eeea7bb5966
10 Nef4060bcaa9c48af9f4957a226531904
11 Nf8adf090169f426fa83d806fda5077c8
12 Nfdbd53dcc9044942be3869cd16857e4a
13 anzsrc-for:06
14 anzsrc-for:0604
15 schema:author Nc580392f58cc4760b4e5e48673f77156
16 schema:citation sg:pub.10.1007/s10096-011-1471-z
17 https://app.dimensions.ai/details/publication/pub.1074364313
18 https://doi.org/10.1016/j.diagmicrobio.2010.11.011
19 https://doi.org/10.1016/j.ijantimicag.2012.07.016
20 https://doi.org/10.1016/j.ijmm.2011.09.003
21 https://doi.org/10.1016/j.mimet.2005.03.018
22 https://doi.org/10.1016/s0140-6736(10)61458-4
23 https://doi.org/10.1016/s0147-619x(02)00107-5
24 https://doi.org/10.1016/s0195-6701(97)90163-8
25 https://doi.org/10.1056/nejmsa0810119
26 https://doi.org/10.1086/501620
27 https://doi.org/10.1089/mdr.2010.0132
28 https://doi.org/10.1093/jac/dkl309
29 https://doi.org/10.1093/jac/dkp194
30 https://doi.org/10.1093/jac/dkq347
31 https://doi.org/10.1093/jac/dkq415
32 https://doi.org/10.1111/1469-0691.12339
33 https://doi.org/10.1111/j.1469-0691.2011.03730.x
34 https://doi.org/10.1111/j.1469-0691.2012.03805.x
35 https://doi.org/10.1111/j.1600-0463.2007.apm_547.x
36 https://doi.org/10.1128/aac.02824-14
37 https://doi.org/10.1128/aac.48.4.1249-1255.2004
38 https://doi.org/10.1128/cmr.00016-09
39 https://doi.org/10.1128/cmr.14.4.933-951.2001
40 https://doi.org/10.1371/journal.pone.0005958
41 https://doi.org/10.2471/blt.11.088179
42 https://doi.org/10.3201/eid1402.070350
43 https://doi.org/10.3201/eid1701.100244
44 schema:datePublished 2015-12
45 schema:datePublishedReg 2015-12-01
46 schema:description BACKGROUND: Surgical-site infection is the most frequent health care-associated infection in the developing world, with a strikingly higher prevalence than in developed countries We studied the prevalence of resistance to antibiotics in Enterobacteriaceae isolates from surgical-site infections collected in three major tertiary care centres in Bangui, Central African Republic. We also studied the genetic basis for antibiotic resistance and the genetic background of third-generation cephalosporin-resistant (3GC-R) Enterobacteriaceae. RESULTS: Between April 2011 and April 2012, 195 patients with nosocomial surgical-site infections were consecutively recruited into the study at five surgical departments in three major tertiary care centres. Of the 165 bacterial isolates collected, most were Enterobacteriaceae (102/165, 61.8%). Of these, 65/102 (63.7%) were 3GC-R, which were characterized for resistance gene determinants and genetic background. The bla CTX-M-15 and aac(6')-Ib-cr genes were detected in all strains, usually associated with qnr genes (98.5%). Escherichia coli, the most commonly recovered species (33/65, 50.8%), occurred in six different sequence types, including the pandemic B2-O25b-ST131 group (12/33, 36.4%). Resistance transfer was studied in one representative strain of the resistance gene content in each repetitive extragenic palindromic and enterobacterial repetitive intergenic consensus sequence-PCR banding pattern. Plasmids were characterized by PCR-based replicon typing and sub-typing schemes. In most isolates (18/27, 66.7%), bla CTX-M-15 genes were found in incompatibility groups F/F31:A4:B1 and F/F36:A4:B1 conjugative plasmids. Horizontal transfer of both plasmids is probably an important mechanism for the spread of bla CTX-M-15 among Enterobacteriaceae species and hospitals. The presence of sets of antibiotic resistance genes in these two plasmids indicates their capacity for gene rearrangement and their evolution into new variants. CONCLUSIONS: Diverse modes are involved in transmission of resistance, plasmid dissemination probably playing a major role.
47 schema:genre research_article
48 schema:inLanguage en
49 schema:isAccessibleForFree true
50 schema:isPartOf Nd9d790d59b14456297992498a0f7f571
51 Ne2c449ae9cbc4e66bcc8f7748e03b8bc
52 sg:journal.1024253
53 schema:name Dissemination of IncF-type plasmids in multiresistant CTX-M-15-producing Enterobacteriaceae isolates from surgical-site infections in Bangui, Central African Republic
54 schema:pagination 15
55 schema:productId N2faa21873e4846ed9d52989ab2353283
56 N6f664f655dec4d6088c2a95a98d8f3ab
57 N7e4f937e6a85453d8c3a76ee2be1c4ae
58 N90b7770e553a4a6a837e8b9b78ff769f
59 Na5fa36a094d743aa92964fd759f2a9c6
60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014284925
61 https://doi.org/10.1186/s12866-015-0348-1
62 schema:sdDatePublished 2019-04-11T09:57
63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
64 schema:sdPublisher N6e0cef9081e24f7eb90b94e4d9b951b9
65 schema:url http://link.springer.com/10.1186%2Fs12866-015-0348-1
66 sgo:license sg:explorer/license/
67 sgo:sdDataset articles
68 rdf:type schema:ScholarlyArticle
69 N026460c5dd7f4d8f818eb5ddbf7a8f73 rdf:first sg:person.016403731464.53
70 rdf:rest N439ec50a68b74f08b0fe5006f323b85a
71 N104981897f6d46e2880f0479b3389e13 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Surgical Wound Infection
73 rdf:type schema:DefinedTerm
74 N1c4c4e061b8742838e295391f31ae911 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Polymerase Chain Reaction
76 rdf:type schema:DefinedTerm
77 N2faa21873e4846ed9d52989ab2353283 schema:name dimensions_id
78 schema:value pub.1014284925
79 rdf:type schema:PropertyValue
80 N3538fcb799174f38a78d31d987042686 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Gene Transfer, Horizontal
82 rdf:type schema:DefinedTerm
83 N3bd2c07d945f4aebb21177dee713b63a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Enterobacteriaceae Infections
85 rdf:type schema:DefinedTerm
86 N3d339fc9be5349119cae7daba5628e76 schema:name Hôpital de l’Amitié, Department of General Surgery, Avenue de l’Independance, Bangui, Central African Republic
87 rdf:type schema:Organization
88 N4273a4287a234b10a5231d4d9e4b9aa1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name DNA, Bacterial
90 rdf:type schema:DefinedTerm
91 N439ec50a68b74f08b0fe5006f323b85a rdf:first sg:person.01276276114.28
92 rdf:rest rdf:nil
93 N542b7cb422c4400e96616b90cd1e09ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name beta-Lactamases
95 rdf:type schema:DefinedTerm
96 N55dbb1e0afc74f958f92b1892a468ddc rdf:first sg:person.01310336517.29
97 rdf:rest N710e4acff9944ae8a11417fdaf6ac7a7
98 N6e0cef9081e24f7eb90b94e4d9b951b9 schema:name Springer Nature - SN SciGraph project
99 rdf:type schema:Organization
100 N6f664f655dec4d6088c2a95a98d8f3ab schema:name readcube_id
101 schema:value 440d652b0d6f9a4b6d65f9546b53249cdb30ff02d9dbe863004d2482415b7694
102 rdf:type schema:PropertyValue
103 N710e4acff9944ae8a11417fdaf6ac7a7 rdf:first sg:person.0641720260.05
104 rdf:rest Na278be63d080474fab7de7a6113f4a45
105 N712861cc103b49679c514a4ae5f58d42 schema:name Hôpital Communautaire, Department of Orthopaedic Surgery, Avenue des Martyrs, Bangui, Central African Republic
106 rdf:type schema:Organization
107 N767c7136047b4ded9b7f440068f52fed rdf:first sg:person.01153764400.62
108 rdf:rest N856b5c84fc664474a1ed9d9d7120b0f3
109 N79f086db99b140e49c40fc43de5b7029 schema:name Hôpital de l’Amitié, Department of Gynaecology and Obstetrics, Avenue de l’Independance, Bangui, Central African Republic
110 rdf:type schema:Organization
111 N7e4f937e6a85453d8c3a76ee2be1c4ae schema:name pubmed_id
112 schema:value 25648151
113 rdf:type schema:PropertyValue
114 N856b5c84fc664474a1ed9d9d7120b0f3 rdf:first sg:person.013017672225.56
115 rdf:rest N026460c5dd7f4d8f818eb5ddbf7a8f73
116 N90b7770e553a4a6a837e8b9b78ff769f schema:name doi
117 schema:value 10.1186/s12866-015-0348-1
118 rdf:type schema:PropertyValue
119 N95e085a63d214874b750cf3f10b38d1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Enterobacteriaceae
121 rdf:type schema:DefinedTerm
122 Na278be63d080474fab7de7a6113f4a45 rdf:first sg:person.01306326377.96
123 rdf:rest Nbcd7aa897b904ca687bf6f2a4201f911
124 Na347fbebecb24c858e37f7931adc786a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Central African Republic
126 rdf:type schema:DefinedTerm
127 Na5fa36a094d743aa92964fd759f2a9c6 schema:name nlm_unique_id
128 schema:value 100966981
129 rdf:type schema:PropertyValue
130 Nac4e531cdde84725b9251eeea7bb5966 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Humans
132 rdf:type schema:DefinedTerm
133 Nbcd7aa897b904ca687bf6f2a4201f911 rdf:first sg:person.01046120114.11
134 rdf:rest Ne602be0aebaf4c50af2703049c4baadd
135 Nc580392f58cc4760b4e5e48673f77156 rdf:first sg:person.01205573056.81
136 rdf:rest N55dbb1e0afc74f958f92b1892a468ddc
137 Nd998e531dbbf4d91b104e8cd8d5c06e7 schema:name Complexe Pédiatrique, Department of Paediatric Surgery, Avenue de l’Independance, Bangui, Central African Republic
138 rdf:type schema:Organization
139 Nd9d790d59b14456297992498a0f7f571 schema:volumeNumber 15
140 rdf:type schema:PublicationVolume
141 Ne2c449ae9cbc4e66bcc8f7748e03b8bc schema:issueNumber 1
142 rdf:type schema:PublicationIssue
143 Ne602be0aebaf4c50af2703049c4baadd rdf:first sg:person.01126025365.83
144 rdf:rest N767c7136047b4ded9b7f440068f52fed
145 Nef4060bcaa9c48af9f4957a226531904 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Sequence Analysis, DNA
147 rdf:type schema:DefinedTerm
148 Nf8adf090169f426fa83d806fda5077c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Plasmids
150 rdf:type schema:DefinedTerm
151 Nfdbd53dcc9044942be3869cd16857e4a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Tertiary Care Centers
153 rdf:type schema:DefinedTerm
154 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
155 schema:name Biological Sciences
156 rdf:type schema:DefinedTerm
157 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
158 schema:name Genetics
159 rdf:type schema:DefinedTerm
160 sg:journal.1024253 schema:issn 1471-2180
161 schema:name BMC Microbiology
162 rdf:type schema:Periodical
163 sg:person.01046120114.11 schema:affiliation https://www.grid.ac/institutes/grid.418512.b
164 schema:familyName Mbecko
165 schema:givenName Jean-Robert
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046120114.11
167 rdf:type schema:Person
168 sg:person.01126025365.83 schema:affiliation N3d339fc9be5349119cae7daba5628e76
169 schema:familyName Nghario
170 schema:givenName Luc
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126025365.83
172 rdf:type schema:Person
173 sg:person.01153764400.62 schema:affiliation N79f086db99b140e49c40fc43de5b7029
174 schema:familyName Serdouma
175 schema:givenName Eugene
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153764400.62
177 rdf:type schema:Person
178 sg:person.01205573056.81 schema:affiliation https://www.grid.ac/institutes/grid.418512.b
179 schema:familyName Rafaï
180 schema:givenName Clotaire
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205573056.81
182 rdf:type schema:Person
183 sg:person.01276276114.28 schema:affiliation https://www.grid.ac/institutes/grid.418512.b
184 schema:familyName Breurec
185 schema:givenName Sebastien
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276276114.28
187 rdf:type schema:Person
188 sg:person.013017672225.56 schema:affiliation N712861cc103b49679c514a4ae5f58d42
189 schema:familyName Tekpa
190 schema:givenName Bertrand
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013017672225.56
192 rdf:type schema:Person
193 sg:person.01306326377.96 schema:affiliation Nd998e531dbbf4d91b104e8cd8d5c06e7
194 schema:familyName Gaudeuille
195 schema:givenName Alfred
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306326377.96
197 rdf:type schema:Person
198 sg:person.01310336517.29 schema:affiliation https://www.grid.ac/institutes/grid.418512.b
199 schema:familyName Frank
200 schema:givenName Thierry
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01310336517.29
202 rdf:type schema:Person
203 sg:person.016403731464.53 schema:affiliation https://www.grid.ac/institutes/grid.418511.8
204 schema:familyName Garin
205 schema:givenName Benoit
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016403731464.53
207 rdf:type schema:Person
208 sg:person.0641720260.05 schema:affiliation https://www.grid.ac/institutes/grid.418512.b
209 schema:familyName Manirakiza
210 schema:givenName Alexandre
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641720260.05
212 rdf:type schema:Person
213 sg:pub.10.1007/s10096-011-1471-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1002586959
214 https://doi.org/10.1007/s10096-011-1471-z
215 rdf:type schema:CreativeWork
216 https://app.dimensions.ai/details/publication/pub.1074364313 schema:CreativeWork
217 https://doi.org/10.1016/j.diagmicrobio.2010.11.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025033090
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/j.ijantimicag.2012.07.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029961817
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/j.ijmm.2011.09.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000264700
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/j.mimet.2005.03.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009250078
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/s0140-6736(10)61458-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035917977
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/s0147-619x(02)00107-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027921584
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/s0195-6701(97)90163-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043914218
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1056/nejmsa0810119 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003155860
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1086/501620 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027071356
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1089/mdr.2010.0132 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059297764
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1093/jac/dkl309 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032878174
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1093/jac/dkp194 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026796252
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1093/jac/dkq347 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048093799
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1093/jac/dkq415 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007252053
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1111/1469-0691.12339 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029828675
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1111/j.1469-0691.2011.03730.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1049566366
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1111/j.1469-0691.2012.03805.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1040630788
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1111/j.1600-0463.2007.apm_547.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014282887
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1128/aac.02824-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038451119
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1128/aac.48.4.1249-1255.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005036346
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1128/cmr.00016-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053076235
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1128/cmr.14.4.933-951.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013993524
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1371/journal.pone.0005958 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042501168
262 rdf:type schema:CreativeWork
263 https://doi.org/10.2471/blt.11.088179 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070837861
264 rdf:type schema:CreativeWork
265 https://doi.org/10.3201/eid1402.070350 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014602679
266 rdf:type schema:CreativeWork
267 https://doi.org/10.3201/eid1701.100244 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071123111
268 rdf:type schema:CreativeWork
269 https://www.grid.ac/institutes/grid.418511.8 schema:alternateName Institut Pasteur de Madagascar
270 schema:name Institut Pasteur, Laboratory of Bacteriology, BP 1274, Antananarivo, Madagascar
271 rdf:type schema:Organization
272 https://www.grid.ac/institutes/grid.418512.b schema:alternateName Institut Pasteur de Bangui
273 schema:name Institut Pasteur, Laboratory of Bacteriology, Avenue de l’Independance, BP 923, Bangui, Central African Republic
274 Institut Pasteur, Unit of Epidemiology, Avenue de l’Independance, BP 923, Bangui, Central African Republic
275 University of Antilles, Faculty of Medecine, Pointe-à-Pitre, French West Indies
276 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...