Proteome sequencing and analysis of Ophiocordyceps sinensis at different culture periods View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2020-12-11

AUTHORS

Bo Zhang, Bo Li, Xiao-Hui Men, Zhe-Wen Xu, Hui Wu, Xiang-Tian Qin, Feng Xu, Yi Teng, Shui-Jin Yuan, Li-Qun Jin, Zhi-Qiang Liu, Yu-Guo Zheng

ABSTRACT

BACKGROUND: Ophiocordyceps sinensis is an important traditional Chinese medicine for its comprehensive active ingredients, such as cordycepin, cordycepic acid, and Cordyceps polysaccharide. O. sinensis zjut, a special strain isolated from O. sinensis, has similar pharmacological functions to wild O. sinensis. Currently, O. sinensis with artificial cultivation has been widely studied, but systematic fundamental research at protein levels has not been determined. RESULTS: Proteomes of O. sinensis zjut at different culture periods (growth period, 3rd day; pre-stable period, 6th day; and stable period, 9th day) were relatively quantified by relative isotope markers and absolute quantitative technology. In total, 4005 proteins were obtained and further annotated with Gene Ontology, Kyoto Encyclopedia of Genes and Genomes database. Based on the result of the annotations, metabolic pathways of active ingredients, amino acids and fatty acid were constructed, and the related enzymes were exhibited. Subsequently, comparative proteomics of O. sinensis zjut identified the differentially expressed proteins (DEPs) by growth in different culture periods, to find the important proteins involved in metabolic pathways of active ingredients. 605 DEPs between 6d-VS-3d, 1188 DEPs between 9d-VS-3d, and 428 DEPs between 9d-VS-6d were obtained, respectively. CONCLUSION: This work provided scientific basis to study protein profile and comparison of protein expression levels of O. sinensis zjut, and it will be helpful for metabolic engineering works to active ingredients for exploration, application and improvement of this fungus. More... »

PAGES

886

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12864-020-07298-z

DOI

http://dx.doi.org/10.1186/s12864-020-07298-z

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https://app.dimensions.ai/details/publication/pub.1133445731

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33308160


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61 genome database
62 growth
63 important proteins
64 important traditional Chinese medicine
65 improvement
66 ingredients
67 isotope markers
68 levels
69 markers
70 medicine
71 metabolic engineering work
72 metabolic pathways
73 ontology
74 pathway
75 period
76 pharmacological functions
77 polysaccharides
78 profile
79 protein
80 protein expression levels
81 protein levels
82 protein profiles
83 proteome
84 proteome sequencing
85 proteomics
86 quantitative technology
87 related enzymes
88 relative isotope markers
89 research
90 results
91 scientific basis
92 sequencing
93 similar pharmacological functions
94 sinensis
95 sinensis zjut
96 special strain
97 strains
98 systematic fundamental research
99 technology
100 traditional Chinese medicine
101 work
102 zjut
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