The NAC family transcription factor GmNAC42–1 regulates biosynthesis of the anticancer and neuroprotective glyceollins in soybean View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Md Asraful Jahan, Brianna Harris, Matthew Lowery, Katie Coburn, Aniello M. Infante, Ryan J. Percifield, Amanda G. Ammer, Nik Kovinich

ABSTRACT

BACKGROUND: Glyceollins are isoflavonoid-derived pathogen-inducible defense metabolites (phytoalexins) from soybean (Glycine max L. Merr) that have important roles in providing defense against pathogens. They also have impressive anticancer and neuroprotective activities in mammals. Despite their potential usefulness as therapeutics, glyceollins are not economical to synthesize and are biosynthesized only transiently and in low amounts in response to specific stresses. Engineering the regulation of glyceollin biosynthesis may be a promising approach to enhance their bioproduction, yet the transcription factors (TFs) that regulate their biosynthesis have remained elusive. To address this, we first aimed to identify novel abiotic stresses that enhance or suppress the elicitation of glyceollins and then used a comparative transcriptomics approach to search for TF gene candidates that may positively regulate glyceollin biosynthesis. RESULTS: Acidity stress (pH 3.0 medium) and dehydration exerted prolonged (week-long) inductive or suppressive effects on glyceollin biosynthesis, respectively. RNA-seq found that all known biosynthetic genes were oppositely regulated by acidity stress and dehydration, but known isoflavonoid TFs were not. Systemic acquired resistance (SAR) genes were highly enriched in the geneset. We chose to functionally characterize the NAC (NAM/ATAF1/2/CUC2)-family TF GmNAC42-1 that was annotated as an SAR gene and a homolog of the Arabidopsis thaliana (Arabidopsis) indole alkaloid phytoalexin regulator ANAC042. Overexpressing and silencing GmNAC42-1 in elicited soybean hairy roots dramatically enhanced and suppressed the amounts of glyceollin metabolites and biosynthesis gene mRNAs, respectively. Yet, overexpressing GmNAC42-1 in non-elicited hairy roots failed to stimulate the expressions of all biosynthesis genes. Thus, GmNAC42-1 was necessary but not sufficient to activate all biosynthesis genes on its own, suggesting an important role in the glyceollin gene regulatory network (GRN). The GmNAC42-1 protein directly bound the promoters of biosynthesis genes IFS2 and G4DT in the yeast one-hybrid (Y1H) system. CONCLUSIONS: Acidity stress is a novel elicitor and dehydration is a suppressor of glyceollin biosynthesis. The TF gene GmNAC42-1 is an essential positive regulator of glyceollin biosynthesis. Overexpressing GmNAC42-1 in hairy roots can be used to increase glyceollin yields > 10-fold upon elicitation. Thus, manipulating the expressions of glyceollin TFs is an effective strategy for enhancing the bioproduction of glyceollins in soybean. More... »

PAGES

149

References to SciGraph publications

  • 2013-03. Effect of mineral composition and medium pH on Scots pine tolerance to toxic effect of zinc ions in RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
  • 2010-10. Differential expression analysis for sequence count data in GENOME BIOLOGY
  • 2003-02. Quantification of camalexin in several accessions ofArabidopsis thaliana following inductions withPeronospora parasitica and UV-B irradiation in PHYTOPARASITICA
  • 2017-12. OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability in SCIENTIFIC REPORTS
  • 2017-12. Soybean cyclophilin GmCYP1 interacts with an isoflavonoid regulator GmMYB176 in SCIENTIFIC REPORTS
  • 2011-07. Protein carbonylation in barley seedling roots caused by aluminum and proton toxicity is suppressed by salicylic acid in RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
  • 2016-11. Expression of a Grapevine NAC Transcription Factor Gene Is Induced in Response to Powdery Mildew Colonization in Salicylic Acid-Independent Manner in SCIENTIFIC REPORTS
  • 2013-06. Metabolism and aluminum accumulation in Plantago almogravensis and P. algarbiensis in response to low pH and aluminum stress in BIOLOGIA PLANTARUM
  • 1985-02. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter in NATURE
  • 1939-11. Physiologisch-genetische Untersuchungen über die Resistenz der Kartoffel gegenüber Phytophthora infestans in THE SCIENCE OF NATURE
  • 2015-12. Targeted genome modifications in soybean with CRISPR/Cas9 in BMC BIOTECHNOLOGY
  • 2013-02. AtMYB14 Regulates Cold Tolerance in Arabidopsis in PLANT MOLECULAR BIOLOGY REPORTER
  • 2016-08. Identification and characterization of a novel NAC-like gene in chrysanthemum (Dendranthema lavandulifolium) in PLANT CELL REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12864-019-5524-5

    DOI

    http://dx.doi.org/10.1186/s12864-019-5524-5

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112259565

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30786857


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