High throughput small RNA and transcriptome sequencing reveal capacitation-related microRNAs and mRNA in boar sperm View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-10-11

AUTHORS

Yuan Li, Rong-Hong Li, Ming-Xia Ran, Yan Zhang, Kai Liang, Ying-Nan Ren, Wen-Cheng He, Ming Zhang, Guang-Bin Zhou, Izhar Hyder Qazi, Chang-Jun Zeng

ABSTRACT

BACKGROUND: Capacitation, a prerequisite for oocyte fertilization, is a complex process involving series of structural and functional changes in sperms such as membrane modifications, modulation of enzyme activities, and protein phosphorylation. In order to penetrate and fertilize an oocyte, mammalian sperms must undergo capacitation. Nevertheless, the process of sperm capacitation remains poorly understood and requires further elucidation. In the current study, via high throughput sequencing, we identified and explored the differentially expressed microRNAs (miRNAs) and mRNAs involved in boar sperm capacitation. RESULTS: We identified a total of 5342 mRNAs and 204 miRNAs that were differentially expressed in fresh and capacitated boar sperms. From these, 12 miRNAs (8 known and 4 newly identified miRNAs) and their differentially expressed target mRNAs were found to be involved in sperm capacitation-related PI3K-Akt, MAPK, cAMP-PKA and Ca2+signaling pathways. CONCLUSIONS: Our study is first to provide the complete miRNA and transcriptome profiles of boar sperm. Our findings provide important insights for the understanding of the RNA profile in boar sperm and future elucidation of the underlying molecular mechanism relevant to mammalian sperm capacitation. More... »

PAGES

736

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12864-018-5132-9

DOI

http://dx.doi.org/10.1186/s12864-018-5132-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1107532210

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30305024


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42 cAMP-PKA
43 capacitation
44 capacitation-related PI3K-Akt
45 capacitation-related microRNAs
46 changes
47 complete miRNA
48 complex process
49 current study
50 elucidation
51 enzyme activity
52 fertilization
53 findings
54 functional changes
55 further elucidation
56 future elucidation
57 high-throughput sequencing
58 high-throughput small RNA
59 important insights
60 insights
61 mRNA
62 mammalian sperm
63 mammalian sperm capacitation
64 mechanism
65 membrane modification
66 miRNA
67 miRNAs
68 microRNAs
69 modification
70 modulation
71 molecular mechanisms
72 oocyte fertilization
73 order
74 pathway
75 phosphorylation
76 prerequisite
77 process
78 profile
79 protein phosphorylation
80 sequencing
81 series
82 small RNAs
83 sperm
84 sperm capacitation
85 sperm capacitation-related PI3K-Akt
86 study
87 target mRNAs
88 throughput sequencing
89 throughput small RNA
90 total
91 transcriptome profiles
92 transcriptome sequencing
93 understanding
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