Transcriptome profiling of the honeybee parasite Varroa destructor provides new biological insights into the mite adult life cycle View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-05-04

AUTHORS

Fanny Mondet, Andrea Rau, Christophe Klopp, Marine Rohmer, Dany Severac, Yves Le Conte, Cedric Alaux

ABSTRACT

BackgroundThe parasite Varroa destructor represents a significant threat to honeybee colonies. Indeed, development of Varroa infestation within colonies, if left untreated, often leads to the death of the colony. Although its impact on bees has been extensively studied, less is known about its biology and the functional processes governing its adult life cycle and adaptation to its host. We therefore developed a full life cycle transcriptomic catalogue in adult Varroa females and included pairwise comparisons with males, artificially-reared and non-reproducing females (10 life cycle stages and conditions in total).ResultsExtensive remodeling of the Varroa transcriptome was observed, with an upregulation of energetic and chitin metabolic processes during the initial and final phases of the life cycle (e.g. phoretic and post-oviposition stages), whereas during reproductive stages in brood cells genes showing functions related to transcriptional regulation were overexpressed. Several neurotransmitter and neuropeptide receptors involved in behavioural regulation, as well as active compounds of salivary glands, were also expressed at a higher level outside the reproductive stages. No difference was detected between artificially-reared phoretic females and their counterparts in colonies, or between females who failed to reproduce and females who successfully reproduced, indicating that phoretic individuals can be reared outside host colonies without impacting their physiology and that mechanisms underlying reproductive failure occur before oogenesis.ConclusionsWe discuss how these new findings reveal the remarkable adaptation of Varroa to its host biology and notably to the switch from living on adults to reproducing in sealed brood cells. By spanning the entire adult life cycle, our work captures the dynamic changes in the parasite gene expression and serves as a unique resource for deciphering Varroa biology and identifying new targets for mite control. More... »

PAGES

328

References to SciGraph publications

  • 2016-11-16. Differential gene expression in Varroa jacobsoni mites following a host shift to European honey bees (Apis mellifera) in BMC GENOMICS
  • 2010-10-25. Genomic survey of the ectoparasitic mite Varroa destructor, a major pest of the honey bee Apis mellifera in BMC GENOMICS
  • 2013-02-20. Artificial feeding of Varroa destructor through a chitosan membrane: a tool for studying the host-microparasite relationship in EXPERIMENTAL AND APPLIED ACAROLOGY
  • 2016-11-24. Two novel viruses associated with the Apis mellifera pathogenic mite Varroa destructor in SCIENTIFIC REPORTS
  • 1994. A technique for reproduction of Varroa jacobsoni Oud under laboratory conditions in APIDOLOGIE
  • 1982. DETERMINATION OF PROTEOLYTIC ACTIVITY IN VARROA JACOBSONI AN ECTOPARASITIC HEMOPHAGOUS MITE OF HONEY BEES (APIS sp.) in APIDOLOGIE
  • 2016-06-16. Suppressing male spermatogenesis-associated protein 5-like gene expression reduces vitellogenin gene expression and fecundity in Nilaparvata lugens Stål in SCIENTIFIC REPORTS
  • 2016-02-09. Genomic insights into the Ixodes scapularis tick vector of Lyme disease in NATURE COMMUNICATIONS
  • 2004-07. The energy and nutritional demand of the parasitic life of the mite Varroa destructor in APIDOLOGIE
  • 2014-12-05. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 in GENOME BIOLOGY
  • 2007-08-22. Hitting the on switch in NATURE
  • 2009. ggplot2, Elegant Graphics for Data Analysis in NONE
  • 1997-05. High Humidity in the Honey Bee (Apis mellifera L.) Brood Nest Limits Reproduction of the Parasitic Mite Varroa jacobsoni Oud. in THE SCIENCE OF NATURE
  • 2015-09-11. In-depth proteomic analysis of Varroa destructor: Detection of DWV-complex, ABPV, VdMLV and honeybee proteins in the mite in SCIENTIFIC REPORTS
  • 2017-10-12. Chemosensing of honeybee parasite, Varroa destructor: Transcriptomic analysis in SCIENTIFIC REPORTS
  • 2000-09. Toluidine blue staining as a rapid measure for initiation of oocyte growth and fertility in Varroa jacobsoni Oud in APIDOLOGIE
  • 2010-04-12. Varroa mites and honey bee health: can Varroa explain part of the colony losses? in APIDOLOGIE
  • 2010-03-26. The four hexamerin genes in the honey bee: structure, molecular evolution and function deduced from expression patterns in queens, workers and drones in BMC MOLECULAR AND CELL BIOLOGY
  • 2000-05. Control of Varroa jacobsoni Oud. resistant to fluvalinate and amitraz using coumaphos in APIDOLOGIE
  • 2001-01. First detection of strains of Varroa destructor resistant to coumaphos. Results of laboratory tests and field trials in APIDOLOGIE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12864-018-4668-z

    DOI

    http://dx.doi.org/10.1186/s12864-018-4668-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1103810958

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29728057


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Arthropod Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bees", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Female", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Profiling", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Host-Parasite Interactions", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Life Cycle Stages", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Male", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Reproduction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transcriptome", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Varroidae", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Vitellogenins", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "INRA, UR 406 Abeilles et Environnement, 84914, Avignon, France", 
              "id": "http://www.grid.ac/institutes/grid.507621.7", 
              "name": [
                "INRA, UR 406 Abeilles et Environnement, 84914, Avignon, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mondet", 
            "givenName": "Fanny", 
            "id": "sg:person.01057552316.57", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057552316.57"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "INRA, UMR 1313 GABI G\u00e9n\u00e9tique Animale et Biologie Int\u00e9grative, 78350, Jouy-en-Josas, France", 
              "id": "http://www.grid.ac/institutes/grid.417961.c", 
              "name": [
                "INRA, UMR 1313 GABI G\u00e9n\u00e9tique Animale et Biologie Int\u00e9grative, 78350, Jouy-en-Josas, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rau", 
            "givenName": "Andrea", 
            "id": "sg:person.01202456031.91", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202456031.91"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "INRA, Genotoul Bioinfo, UR 875 MIAT Math\u00e9matiques et Informatique Appliqu\u00e9es de Toulouse, 31326, Castanet-Tolosan, France", 
              "id": "http://www.grid.ac/institutes/grid.507621.7", 
              "name": [
                "INRA, Genotoul Bioinfo, UR 875 MIAT Math\u00e9matiques et Informatique Appliqu\u00e9es de Toulouse, 31326, Castanet-Tolosan, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Klopp", 
            "givenName": "Christophe", 
            "id": "sg:person.0635515327.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0635515327.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institut de G\u00e9nomique Fonctionnelle, UMR 5203 CNRS, U661 INSERM, Universit\u00e9s Montpellier 1 & 2, 34094, Montpellier, France", 
              "id": "http://www.grid.ac/institutes/grid.461890.2", 
              "name": [
                "Institut de G\u00e9nomique Fonctionnelle, UMR 5203 CNRS, U661 INSERM, Universit\u00e9s Montpellier 1 & 2, 34094, Montpellier, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rohmer", 
            "givenName": "Marine", 
            "id": "sg:person.01074077271.88", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074077271.88"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institut de G\u00e9nomique Fonctionnelle, UMR 5203 CNRS, U661 INSERM, Universit\u00e9s Montpellier 1 & 2, 34094, Montpellier, France", 
              "id": "http://www.grid.ac/institutes/grid.461890.2", 
              "name": [
                "Institut de G\u00e9nomique Fonctionnelle, UMR 5203 CNRS, U661 INSERM, Universit\u00e9s Montpellier 1 & 2, 34094, Montpellier, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Severac", 
            "givenName": "Dany", 
            "id": "sg:person.01142671607.49", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142671607.49"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "INRA, UR 406 Abeilles et Environnement, 84914, Avignon, France", 
              "id": "http://www.grid.ac/institutes/grid.507621.7", 
              "name": [
                "INRA, UR 406 Abeilles et Environnement, 84914, Avignon, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Le Conte", 
            "givenName": "Yves", 
            "id": "sg:person.01223471277.66", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01223471277.66"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "INRA, UR 406 Abeilles et Environnement, 84914, Avignon, France", 
              "id": "http://www.grid.ac/institutes/grid.507621.7", 
              "name": [
                "INRA, UR 406 Abeilles et Environnement, 84914, Avignon, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Alaux", 
            "givenName": "Cedric", 
            "id": "sg:person.01170206413.18", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170206413.18"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1051/apido:2004032", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029395057", 
              "https://doi.org/10.1051/apido:2004032"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1051/apido:2001110", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050363372", 
              "https://doi.org/10.1051/apido:2001110"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s001140050382", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044028385", 
              "https://doi.org/10.1007/s001140050382"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep37710", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037803667", 
              "https://doi.org/10.1038/srep37710"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1051/apido/2010017", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021968725", 
              "https://doi.org/10.1051/apido/2010017"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms10507", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006746008", 
              "https://doi.org/10.1038/ncomms10507"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2199-11-23", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027106797", 
              "https://doi.org/10.1186/1471-2199-11-23"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-0-387-98141-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041188628", 
              "https://doi.org/10.1007/978-0-387-98141-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13059-014-0550-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015222646", 
              "https://doi.org/10.1186/s13059-014-0550-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep28111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009172864", 
              "https://doi.org/10.1038/srep28111"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep13907", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025775265", 
              "https://doi.org/10.1038/srep13907"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1051/apido:19940608", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006104333", 
              "https://doi.org/10.1051/apido:19940608"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1051/apido:2000146", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041265737", 
              "https://doi.org/10.1051/apido:2000146"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2164-11-602", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026134964", 
              "https://doi.org/10.1186/1471-2164-11-602"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1051/apido:19820405", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029797080", 
              "https://doi.org/10.1051/apido:19820405"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10493-013-9675-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013574133", 
              "https://doi.org/10.1007/s10493-013-9675-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-017-13167-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092119384", 
              "https://doi.org/10.1038/s41598-017-13167-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/448855a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031420767", 
              "https://doi.org/10.1038/448855a"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1051/apido:2000134", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018571762", 
              "https://doi.org/10.1051/apido:2000134"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12864-016-3130-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014951689", 
              "https://doi.org/10.1186/s12864-016-3130-3"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2018-05-04", 
        "datePublishedReg": "2018-05-04", 
        "description": "BackgroundThe parasite Varroa destructor represents a significant threat to honeybee colonies. Indeed, development of Varroa infestation within colonies, if left untreated, often leads to the death of the colony. Although its impact on bees has been extensively studied, less is known about its biology and the functional processes governing its adult life cycle and adaptation to its host. We therefore developed a full life cycle transcriptomic catalogue in adult Varroa females and included pairwise comparisons with males, artificially-reared and non-reproducing females (10 life cycle stages and conditions in total).ResultsExtensive remodeling of the Varroa transcriptome was observed, with an upregulation of energetic and chitin metabolic processes during the initial and final phases of the life cycle (e.g. phoretic and post-oviposition stages), whereas during reproductive stages in brood cells genes showing functions related to transcriptional regulation were overexpressed. Several neurotransmitter and neuropeptide receptors involved in behavioural regulation, as well as active compounds of salivary glands, were also expressed at a higher level outside the reproductive stages. No difference was detected between artificially-reared phoretic females and their counterparts in colonies, or between females who failed to reproduce and females who successfully reproduced, indicating that phoretic individuals can be reared outside host colonies without impacting their physiology and that mechanisms underlying reproductive failure occur before oogenesis.ConclusionsWe discuss how these new findings reveal the remarkable adaptation of Varroa to its host biology and notably to the switch from living on adults to reproducing in sealed brood cells. By spanning the entire adult life cycle, our work captures the dynamic changes in the parasite gene expression and serves as a unique resource for deciphering Varroa biology and identifying new targets for mite control.", 
        "genre": "article", 
        "id": "sg:pub.10.1186/s12864-018-4668-z", 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1023790", 
            "issn": [
              "1471-2164"
            ], 
            "name": "BMC Genomics", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "19"
          }
        ], 
        "keywords": [
          "parasite Varroa destructor", 
          "Varroa destructor", 
          "reproductive stage", 
          "chitin metabolic process", 
          "honeybee parasite Varroa destructor", 
          "parasite gene expression", 
          "life cycle", 
          "new biological insights", 
          "non-reproducing females", 
          "Varroa biology", 
          "transcriptional regulation", 
          "brood cells", 
          "host biology", 
          "phoretic females", 
          "Varroa females", 
          "host colonies", 
          "adult life cycle", 
          "gene expression", 
          "biological insights", 
          "cell genes", 
          "honeybee colonies", 
          "remarkable adaptation", 
          "metabolic processes", 
          "Varroa infestation", 
          "biology", 
          "functional processes", 
          "neuropeptide receptors", 
          "mite control", 
          "new targets", 
          "colonies", 
          "destructor", 
          "unique resource", 
          "regulation", 
          "significant threat", 
          "transcriptome", 
          "dynamic changes", 
          "oogenesis", 
          "Varroa", 
          "salivary glands", 
          "bees", 
          "adaptation", 
          "genes", 
          "pairwise comparisons", 
          "new findings", 
          "females", 
          "profiling", 
          "physiology", 
          "high levels", 
          "host", 
          "cycle", 
          "expression", 
          "active compounds", 
          "upregulation", 
          "infestation", 
          "remodeling", 
          "cells", 
          "receptors", 
          "stage", 
          "target", 
          "insights", 
          "mechanism", 
          "neurotransmitters", 
          "gland", 
          "switch", 
          "function", 
          "threat", 
          "males", 
          "process", 
          "death", 
          "occurs", 
          "development", 
          "behavioral regulation", 
          "levels", 
          "compounds", 
          "individuals", 
          "changes", 
          "catalogue", 
          "counterparts", 
          "final phase", 
          "resources", 
          "control", 
          "findings", 
          "differences", 
          "comparison", 
          "impact", 
          "ConclusionsWe", 
          "adults", 
          "phase", 
          "work", 
          "failure occur"
        ], 
        "name": "Transcriptome profiling of the honeybee parasite Varroa destructor provides new biological insights into the mite adult life cycle", 
        "pagination": "328", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1103810958"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s12864-018-4668-z"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "29728057"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s12864-018-4668-z", 
          "https://app.dimensions.ai/details/publication/pub.1103810958"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-10-01T06:44", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_790.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1186/s12864-018-4668-z"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12864-018-4668-z'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12864-018-4668-z'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12864-018-4668-z'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12864-018-4668-z'


     

    This table displays all metadata directly associated to this object as RDF triples.

    328 TRIPLES      21 PREDICATES      147 URIs      119 LITERALS      19 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s12864-018-4668-z schema:about N06626f81635147e6a8b53520434b8005
    2 N086cd44eca444bab824bcdd95a6bf837
    3 N286d5978582f497b9e4db51817dfe47a
    4 N2898cce165d5479c8b6b7313f133d3e1
    5 N3289e9d57ee94e1296ef5adc76c14c9f
    6 N54816ed90c5d4afb8ffc7aa7cc15ae6d
    7 N6d03d1d9102647f5b4a21efcd97886d8
    8 N7e8e956c6c184a358ed1efe5ae5a004c
    9 Naa6fd1adc76b446ab3921831dbf70b26
    10 Ndc7e782f828342ee9a6b4c35d14dc652
    11 Nea95776f949a4ca08ad71fedb291da98
    12 Nef354827cfd04dc78f3ec820098b2328
    13 anzsrc-for:06
    14 anzsrc-for:0604
    15 schema:author N7c2c96fc987449e193bddc98c64855de
    16 schema:citation sg:pub.10.1007/978-0-387-98141-3
    17 sg:pub.10.1007/s001140050382
    18 sg:pub.10.1007/s10493-013-9675-9
    19 sg:pub.10.1038/448855a
    20 sg:pub.10.1038/ncomms10507
    21 sg:pub.10.1038/s41598-017-13167-9
    22 sg:pub.10.1038/srep13907
    23 sg:pub.10.1038/srep28111
    24 sg:pub.10.1038/srep37710
    25 sg:pub.10.1051/apido/2010017
    26 sg:pub.10.1051/apido:19820405
    27 sg:pub.10.1051/apido:19940608
    28 sg:pub.10.1051/apido:2000134
    29 sg:pub.10.1051/apido:2000146
    30 sg:pub.10.1051/apido:2001110
    31 sg:pub.10.1051/apido:2004032
    32 sg:pub.10.1186/1471-2164-11-602
    33 sg:pub.10.1186/1471-2199-11-23
    34 sg:pub.10.1186/s12864-016-3130-3
    35 sg:pub.10.1186/s13059-014-0550-8
    36 schema:datePublished 2018-05-04
    37 schema:datePublishedReg 2018-05-04
    38 schema:description BackgroundThe parasite Varroa destructor represents a significant threat to honeybee colonies. Indeed, development of Varroa infestation within colonies, if left untreated, often leads to the death of the colony. Although its impact on bees has been extensively studied, less is known about its biology and the functional processes governing its adult life cycle and adaptation to its host. We therefore developed a full life cycle transcriptomic catalogue in adult Varroa females and included pairwise comparisons with males, artificially-reared and non-reproducing females (10 life cycle stages and conditions in total).ResultsExtensive remodeling of the Varroa transcriptome was observed, with an upregulation of energetic and chitin metabolic processes during the initial and final phases of the life cycle (e.g. phoretic and post-oviposition stages), whereas during reproductive stages in brood cells genes showing functions related to transcriptional regulation were overexpressed. Several neurotransmitter and neuropeptide receptors involved in behavioural regulation, as well as active compounds of salivary glands, were also expressed at a higher level outside the reproductive stages. No difference was detected between artificially-reared phoretic females and their counterparts in colonies, or between females who failed to reproduce and females who successfully reproduced, indicating that phoretic individuals can be reared outside host colonies without impacting their physiology and that mechanisms underlying reproductive failure occur before oogenesis.ConclusionsWe discuss how these new findings reveal the remarkable adaptation of Varroa to its host biology and notably to the switch from living on adults to reproducing in sealed brood cells. By spanning the entire adult life cycle, our work captures the dynamic changes in the parasite gene expression and serves as a unique resource for deciphering Varroa biology and identifying new targets for mite control.
    39 schema:genre article
    40 schema:isAccessibleForFree true
    41 schema:isPartOf N1ecede21d3ae4ed3b058387b6fef2a4d
    42 N2daedb9f4c094ae2a691db8140a82996
    43 sg:journal.1023790
    44 schema:keywords ConclusionsWe
    45 Varroa
    46 Varroa biology
    47 Varroa destructor
    48 Varroa females
    49 Varroa infestation
    50 active compounds
    51 adaptation
    52 adult life cycle
    53 adults
    54 bees
    55 behavioral regulation
    56 biological insights
    57 biology
    58 brood cells
    59 catalogue
    60 cell genes
    61 cells
    62 changes
    63 chitin metabolic process
    64 colonies
    65 comparison
    66 compounds
    67 control
    68 counterparts
    69 cycle
    70 death
    71 destructor
    72 development
    73 differences
    74 dynamic changes
    75 expression
    76 failure occur
    77 females
    78 final phase
    79 findings
    80 function
    81 functional processes
    82 gene expression
    83 genes
    84 gland
    85 high levels
    86 honeybee colonies
    87 honeybee parasite Varroa destructor
    88 host
    89 host biology
    90 host colonies
    91 impact
    92 individuals
    93 infestation
    94 insights
    95 levels
    96 life cycle
    97 males
    98 mechanism
    99 metabolic processes
    100 mite control
    101 neuropeptide receptors
    102 neurotransmitters
    103 new biological insights
    104 new findings
    105 new targets
    106 non-reproducing females
    107 occurs
    108 oogenesis
    109 pairwise comparisons
    110 parasite Varroa destructor
    111 parasite gene expression
    112 phase
    113 phoretic females
    114 physiology
    115 process
    116 profiling
    117 receptors
    118 regulation
    119 remarkable adaptation
    120 remodeling
    121 reproductive stage
    122 resources
    123 salivary glands
    124 significant threat
    125 stage
    126 switch
    127 target
    128 threat
    129 transcriptional regulation
    130 transcriptome
    131 unique resource
    132 upregulation
    133 work
    134 schema:name Transcriptome profiling of the honeybee parasite Varroa destructor provides new biological insights into the mite adult life cycle
    135 schema:pagination 328
    136 schema:productId N1564394caeff465189b3388a23fdffe6
    137 N1fef5dec81eb4b1c88d945a7a9bda6bd
    138 N54520370e26a44ca90e348b62f7cf90f
    139 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103810958
    140 https://doi.org/10.1186/s12864-018-4668-z
    141 schema:sdDatePublished 2022-10-01T06:44
    142 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    143 schema:sdPublisher N38a1da7c84794ada80b2ab33a54666ab
    144 schema:url https://doi.org/10.1186/s12864-018-4668-z
    145 sgo:license sg:explorer/license/
    146 sgo:sdDataset articles
    147 rdf:type schema:ScholarlyArticle
    148 N06626f81635147e6a8b53520434b8005 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Host-Parasite Interactions
    150 rdf:type schema:DefinedTerm
    151 N086cd44eca444bab824bcdd95a6bf837 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    152 schema:name Gene Expression Profiling
    153 rdf:type schema:DefinedTerm
    154 N14cf3d6aab1e4d749b4e9e480701b25a rdf:first sg:person.01074077271.88
    155 rdf:rest N50812ab958aa4fe8955654b1e1cd57aa
    156 N1564394caeff465189b3388a23fdffe6 schema:name dimensions_id
    157 schema:value pub.1103810958
    158 rdf:type schema:PropertyValue
    159 N1ecede21d3ae4ed3b058387b6fef2a4d schema:issueNumber 1
    160 rdf:type schema:PublicationIssue
    161 N1fef5dec81eb4b1c88d945a7a9bda6bd schema:name pubmed_id
    162 schema:value 29728057
    163 rdf:type schema:PropertyValue
    164 N2714d9bb0c444eef9f3f1afaa533e36a rdf:first sg:person.01170206413.18
    165 rdf:rest rdf:nil
    166 N286d5978582f497b9e4db51817dfe47a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    167 schema:name Transcriptome
    168 rdf:type schema:DefinedTerm
    169 N2898cce165d5479c8b6b7313f133d3e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    170 schema:name Arthropod Proteins
    171 rdf:type schema:DefinedTerm
    172 N2daedb9f4c094ae2a691db8140a82996 schema:volumeNumber 19
    173 rdf:type schema:PublicationVolume
    174 N3289e9d57ee94e1296ef5adc76c14c9f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    175 schema:name Varroidae
    176 rdf:type schema:DefinedTerm
    177 N38a1da7c84794ada80b2ab33a54666ab schema:name Springer Nature - SN SciGraph project
    178 rdf:type schema:Organization
    179 N50812ab958aa4fe8955654b1e1cd57aa rdf:first sg:person.01142671607.49
    180 rdf:rest N9eea9aa12ee440ae84c260aae75e8171
    181 N54520370e26a44ca90e348b62f7cf90f schema:name doi
    182 schema:value 10.1186/s12864-018-4668-z
    183 rdf:type schema:PropertyValue
    184 N54816ed90c5d4afb8ffc7aa7cc15ae6d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    185 schema:name Reproduction
    186 rdf:type schema:DefinedTerm
    187 N6d03d1d9102647f5b4a21efcd97886d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    188 schema:name Female
    189 rdf:type schema:DefinedTerm
    190 N7c2c96fc987449e193bddc98c64855de rdf:first sg:person.01057552316.57
    191 rdf:rest Nc4869d14fc7f49cb93bbbdf542604f8e
    192 N7e8e956c6c184a358ed1efe5ae5a004c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    193 schema:name Male
    194 rdf:type schema:DefinedTerm
    195 N9eea9aa12ee440ae84c260aae75e8171 rdf:first sg:person.01223471277.66
    196 rdf:rest N2714d9bb0c444eef9f3f1afaa533e36a
    197 Naa6fd1adc76b446ab3921831dbf70b26 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    198 schema:name Vitellogenins
    199 rdf:type schema:DefinedTerm
    200 Nc4869d14fc7f49cb93bbbdf542604f8e rdf:first sg:person.01202456031.91
    201 rdf:rest Nfec2ce90b8f74ed88aedc8d34196abc4
    202 Ndc7e782f828342ee9a6b4c35d14dc652 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    203 schema:name Bees
    204 rdf:type schema:DefinedTerm
    205 Nea95776f949a4ca08ad71fedb291da98 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    206 schema:name Life Cycle Stages
    207 rdf:type schema:DefinedTerm
    208 Nef354827cfd04dc78f3ec820098b2328 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    209 schema:name Animals
    210 rdf:type schema:DefinedTerm
    211 Nfec2ce90b8f74ed88aedc8d34196abc4 rdf:first sg:person.0635515327.32
    212 rdf:rest N14cf3d6aab1e4d749b4e9e480701b25a
    213 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    214 schema:name Biological Sciences
    215 rdf:type schema:DefinedTerm
    216 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    217 schema:name Genetics
    218 rdf:type schema:DefinedTerm
    219 sg:journal.1023790 schema:issn 1471-2164
    220 schema:name BMC Genomics
    221 schema:publisher Springer Nature
    222 rdf:type schema:Periodical
    223 sg:person.01057552316.57 schema:affiliation grid-institutes:grid.507621.7
    224 schema:familyName Mondet
    225 schema:givenName Fanny
    226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057552316.57
    227 rdf:type schema:Person
    228 sg:person.01074077271.88 schema:affiliation grid-institutes:grid.461890.2
    229 schema:familyName Rohmer
    230 schema:givenName Marine
    231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074077271.88
    232 rdf:type schema:Person
    233 sg:person.01142671607.49 schema:affiliation grid-institutes:grid.461890.2
    234 schema:familyName Severac
    235 schema:givenName Dany
    236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142671607.49
    237 rdf:type schema:Person
    238 sg:person.01170206413.18 schema:affiliation grid-institutes:grid.507621.7
    239 schema:familyName Alaux
    240 schema:givenName Cedric
    241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170206413.18
    242 rdf:type schema:Person
    243 sg:person.01202456031.91 schema:affiliation grid-institutes:grid.417961.c
    244 schema:familyName Rau
    245 schema:givenName Andrea
    246 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202456031.91
    247 rdf:type schema:Person
    248 sg:person.01223471277.66 schema:affiliation grid-institutes:grid.507621.7
    249 schema:familyName Le Conte
    250 schema:givenName Yves
    251 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01223471277.66
    252 rdf:type schema:Person
    253 sg:person.0635515327.32 schema:affiliation grid-institutes:grid.507621.7
    254 schema:familyName Klopp
    255 schema:givenName Christophe
    256 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0635515327.32
    257 rdf:type schema:Person
    258 sg:pub.10.1007/978-0-387-98141-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041188628
    259 https://doi.org/10.1007/978-0-387-98141-3
    260 rdf:type schema:CreativeWork
    261 sg:pub.10.1007/s001140050382 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044028385
    262 https://doi.org/10.1007/s001140050382
    263 rdf:type schema:CreativeWork
    264 sg:pub.10.1007/s10493-013-9675-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013574133
    265 https://doi.org/10.1007/s10493-013-9675-9
    266 rdf:type schema:CreativeWork
    267 sg:pub.10.1038/448855a schema:sameAs https://app.dimensions.ai/details/publication/pub.1031420767
    268 https://doi.org/10.1038/448855a
    269 rdf:type schema:CreativeWork
    270 sg:pub.10.1038/ncomms10507 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006746008
    271 https://doi.org/10.1038/ncomms10507
    272 rdf:type schema:CreativeWork
    273 sg:pub.10.1038/s41598-017-13167-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092119384
    274 https://doi.org/10.1038/s41598-017-13167-9
    275 rdf:type schema:CreativeWork
    276 sg:pub.10.1038/srep13907 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025775265
    277 https://doi.org/10.1038/srep13907
    278 rdf:type schema:CreativeWork
    279 sg:pub.10.1038/srep28111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009172864
    280 https://doi.org/10.1038/srep28111
    281 rdf:type schema:CreativeWork
    282 sg:pub.10.1038/srep37710 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037803667
    283 https://doi.org/10.1038/srep37710
    284 rdf:type schema:CreativeWork
    285 sg:pub.10.1051/apido/2010017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021968725
    286 https://doi.org/10.1051/apido/2010017
    287 rdf:type schema:CreativeWork
    288 sg:pub.10.1051/apido:19820405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029797080
    289 https://doi.org/10.1051/apido:19820405
    290 rdf:type schema:CreativeWork
    291 sg:pub.10.1051/apido:19940608 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006104333
    292 https://doi.org/10.1051/apido:19940608
    293 rdf:type schema:CreativeWork
    294 sg:pub.10.1051/apido:2000134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018571762
    295 https://doi.org/10.1051/apido:2000134
    296 rdf:type schema:CreativeWork
    297 sg:pub.10.1051/apido:2000146 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041265737
    298 https://doi.org/10.1051/apido:2000146
    299 rdf:type schema:CreativeWork
    300 sg:pub.10.1051/apido:2001110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050363372
    301 https://doi.org/10.1051/apido:2001110
    302 rdf:type schema:CreativeWork
    303 sg:pub.10.1051/apido:2004032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029395057
    304 https://doi.org/10.1051/apido:2004032
    305 rdf:type schema:CreativeWork
    306 sg:pub.10.1186/1471-2164-11-602 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026134964
    307 https://doi.org/10.1186/1471-2164-11-602
    308 rdf:type schema:CreativeWork
    309 sg:pub.10.1186/1471-2199-11-23 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027106797
    310 https://doi.org/10.1186/1471-2199-11-23
    311 rdf:type schema:CreativeWork
    312 sg:pub.10.1186/s12864-016-3130-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014951689
    313 https://doi.org/10.1186/s12864-016-3130-3
    314 rdf:type schema:CreativeWork
    315 sg:pub.10.1186/s13059-014-0550-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015222646
    316 https://doi.org/10.1186/s13059-014-0550-8
    317 rdf:type schema:CreativeWork
    318 grid-institutes:grid.417961.c schema:alternateName INRA, UMR 1313 GABI Génétique Animale et Biologie Intégrative, 78350, Jouy-en-Josas, France
    319 schema:name INRA, UMR 1313 GABI Génétique Animale et Biologie Intégrative, 78350, Jouy-en-Josas, France
    320 rdf:type schema:Organization
    321 grid-institutes:grid.461890.2 schema:alternateName Institut de Génomique Fonctionnelle, UMR 5203 CNRS, U661 INSERM, Universités Montpellier 1 & 2, 34094, Montpellier, France
    322 schema:name Institut de Génomique Fonctionnelle, UMR 5203 CNRS, U661 INSERM, Universités Montpellier 1 & 2, 34094, Montpellier, France
    323 rdf:type schema:Organization
    324 grid-institutes:grid.507621.7 schema:alternateName INRA, Genotoul Bioinfo, UR 875 MIAT Mathématiques et Informatique Appliquées de Toulouse, 31326, Castanet-Tolosan, France
    325 INRA, UR 406 Abeilles et Environnement, 84914, Avignon, France
    326 schema:name INRA, Genotoul Bioinfo, UR 875 MIAT Mathématiques et Informatique Appliquées de Toulouse, 31326, Castanet-Tolosan, France
    327 INRA, UR 406 Abeilles et Environnement, 84914, Avignon, France
    328 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...