Development and Genetic Characterization of A Novel Herbicide (Imazethapyr) Tolerant Mutant in Rice (Oryza sativa L.) View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-04-04

AUTHORS

D. Shoba, M. Raveendran, S. Manonmani, S. Utharasu, D. Dhivyapriya, G. Subhasini, S. Ramchandar, R. Valarmathi, Nitasha Grover, S. Gopala Krishnan, A. K. Singh, Pawan Jayaswal, Prashant Kale, M. K. Ramkumar, S. V. Amitha Mithra, T. Mohapatra, Kuldeep Singh, N. K. Singh, N. Sarla, M. S. Sheshshayee, M. K. Kar, S. Robin, R. P. Sharma

ABSTRACT

BACKGROUND: Increased water and labour scarcity in major rice growing areas warrants a shift towards direct seeded rice cultivation under which management of weeds is a major issue. Use of broad spectrum non-selective herbicides is an efficient means to manage weeds. Availability of rice genotypes with complete tolerance against broad-spectrum non-selective herbicides is a pre-requisite for advocating use of such herbicides. In the present study, we developed an EMS induced rice mutant, 'HTM-N22', exhibiting tolerance to a broad spectrum herbicide, 'Imazethapyr', and identified the mutations imparting tolerance to the herbicide. RESULTS: We identified a stable and true breeding rice mutant, HTM-N22 (HTM), tolerant to herbicide, Imazethapyr, from an EMS-mutagenized population of approximately 100,000 M2 plants of an upland rice variety, Nagina 22 (N22). Analysis of inheritance of herbicide tolerance in a cross between Pusa 1656-10-61/HTM showed that this trait is governed by a single dominant gene. To identify the causal gene for Imazethapyr tolerance, bulked segregant analysis (BSA) was followed using microsatellite markers flanking the three putative candidate genes viz., an Acetolactate Synthase (ALS) on chromosome 6 and two Acetohydroxy Acid Synthase (AHAS) genes, one on chromosomes 2 and another on chromosome 4. RM 6844 on chromosome 2 located 0.16 Mbp upstream of AHAS (LOC_Os02g30630) was found to co-segregate with herbicide tolerance. Cloning and sequencing of AHAS (LOC_Os02g30630) from the wild type, N22 and the mutant HTM and their comparison with reference Nipponbare sequence revealed several Single Nucleotide Polymorphisms (SNPs) in the mutant, of which eight resulted in non-synonymous mutations. Three of the eight amino acid substitutions were identical to Nipponbare and hence were not considered as causal changes. Of the five putative candidate SNPs, four were novel (at positions 30, 50, 81 and 152) while the remaining one, S627D was a previously reported mutant, known to result in Imidazolinone tolerance in rice. Of the novel ones, G152E was found to alter the hydrophobicty and abolish an N myristoylation site in the HTM compared to the WT, from reference based modeling and motif prediction studies. CONCLUSIONS: A novel mutant tolerant to the herbicide "Imazethapyr" was developed and characterized for genetic, sequence and protein level variations. This is a HTM in rice without any IPR (Intellectual Property Rights) infringements and hence can be used in rice breeding as a novel genetic stock by the public funded organizations in the country and elsewhere. More... »

PAGES

10

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12284-017-0151-8

DOI

http://dx.doi.org/10.1186/s12284-017-0151-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084513443

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28378144


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/07", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Agricultural and Veterinary Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0703", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Crop and Pasture Production", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Tamil Nadu Agricultural University, Coimbatore, 641003 India", 
          "id": "http://www.grid.ac/institutes/grid.412906.8", 
          "name": [
            "Tamil Nadu Agricultural University, Coimbatore, 641003 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shoba", 
        "givenName": "D.", 
        "id": "sg:person.011422504473.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011422504473.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tamil Nadu Agricultural University, Coimbatore, 641003 India", 
          "id": "http://www.grid.ac/institutes/grid.412906.8", 
          "name": [
            "Tamil Nadu Agricultural University, Coimbatore, 641003 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Raveendran", 
        "givenName": "M.", 
        "id": "sg:person.01101441064.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101441064.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tamil Nadu Agricultural University, Coimbatore, 641003 India", 
          "id": "http://www.grid.ac/institutes/grid.412906.8", 
          "name": [
            "Tamil Nadu Agricultural University, Coimbatore, 641003 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Manonmani", 
        "givenName": "S.", 
        "id": "sg:person.011474431111.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011474431111.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tamil Nadu Agricultural University, Coimbatore, 641003 India", 
          "id": "http://www.grid.ac/institutes/grid.412906.8", 
          "name": [
            "Tamil Nadu Agricultural University, Coimbatore, 641003 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Utharasu", 
        "givenName": "S.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tamil Nadu Agricultural University, Coimbatore, 641003 India", 
          "id": "http://www.grid.ac/institutes/grid.412906.8", 
          "name": [
            "Tamil Nadu Agricultural University, Coimbatore, 641003 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dhivyapriya", 
        "givenName": "D.", 
        "id": "sg:person.014410406473.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014410406473.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tamil Nadu Agricultural University, Coimbatore, 641003 India", 
          "id": "http://www.grid.ac/institutes/grid.412906.8", 
          "name": [
            "Tamil Nadu Agricultural University, Coimbatore, 641003 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Subhasini", 
        "givenName": "G.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tamil Nadu Agricultural University, Coimbatore, 641003 India", 
          "id": "http://www.grid.ac/institutes/grid.412906.8", 
          "name": [
            "Tamil Nadu Agricultural University, Coimbatore, 641003 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ramchandar", 
        "givenName": "S.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tamil Nadu Agricultural University, Coimbatore, 641003 India", 
          "id": "http://www.grid.ac/institutes/grid.412906.8", 
          "name": [
            "Tamil Nadu Agricultural University, Coimbatore, 641003 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Valarmathi", 
        "givenName": "R.", 
        "id": "sg:person.01272001472.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272001472.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India", 
          "id": "http://www.grid.ac/institutes/grid.418196.3", 
          "name": [
            "Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Grover", 
        "givenName": "Nitasha", 
        "id": "sg:person.015531114055.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015531114055.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India", 
          "id": "http://www.grid.ac/institutes/grid.418196.3", 
          "name": [
            "Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Krishnan", 
        "givenName": "S. Gopala", 
        "id": "sg:person.01347002134.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347002134.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India", 
          "id": "http://www.grid.ac/institutes/grid.418196.3", 
          "name": [
            "Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Singh", 
        "givenName": "A. K.", 
        "id": "sg:person.0731132341.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731132341.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi, 110012 India", 
          "id": "http://www.grid.ac/institutes/grid.466936.8", 
          "name": [
            "ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi, 110012 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jayaswal", 
        "givenName": "Pawan", 
        "id": "sg:person.01217171521.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01217171521.65"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi, 110012 India", 
          "id": "http://www.grid.ac/institutes/grid.466936.8", 
          "name": [
            "ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi, 110012 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kale", 
        "givenName": "Prashant", 
        "id": "sg:person.014441664475.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014441664475.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi, 110012 India", 
          "id": "http://www.grid.ac/institutes/grid.466936.8", 
          "name": [
            "ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi, 110012 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ramkumar", 
        "givenName": "M. K.", 
        "id": "sg:person.013661141273.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013661141273.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi, 110012 India", 
          "id": "http://www.grid.ac/institutes/grid.466936.8", 
          "name": [
            "ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi, 110012 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mithra", 
        "givenName": "S. V. Amitha", 
        "id": "sg:person.01240305735.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240305735.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Indian Council of Agriculture Research, New Delhi, 110 001 India", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Indian Council of Agriculture Research, New Delhi, 110 001 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mohapatra", 
        "givenName": "T.", 
        "id": "sg:person.01115266135.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115266135.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Present address: ICAR-National Bureau of Plant Genetic Resources, Pusa, New Delhi, 110012 India", 
          "id": "http://www.grid.ac/institutes/grid.452695.9", 
          "name": [
            "Punjab Agricultural University, Ludhiana, 141004 India", 
            "Present address: ICAR-National Bureau of Plant Genetic Resources, Pusa, New Delhi, 110012 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Singh", 
        "givenName": "Kuldeep", 
        "id": "sg:person.010554113672.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010554113672.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi, 110012 India", 
          "id": "http://www.grid.ac/institutes/grid.466936.8", 
          "name": [
            "ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi, 110012 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Singh", 
        "givenName": "N. K.", 
        "id": "sg:person.01047152735.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047152735.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Indian Institute of Rice Research, Rajendranagar, Hyderabad, 500030 India", 
          "id": "http://www.grid.ac/institutes/grid.464820.c", 
          "name": [
            "Indian Institute of Rice Research, Rajendranagar, Hyderabad, 500030 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sarla", 
        "givenName": "N.", 
        "id": "sg:person.013365020075.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013365020075.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Agricultural Sciences, Bengaluru, 560065 India", 
          "id": "http://www.grid.ac/institutes/grid.413008.e", 
          "name": [
            "University of Agricultural Sciences, Bengaluru, 560065 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sheshshayee", 
        "givenName": "M. S.", 
        "id": "sg:person.011605605461.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011605605461.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Rice Research Institute, Cuttack, Odisha 753006 India", 
          "id": "http://www.grid.ac/institutes/grid.418371.8", 
          "name": [
            "National Rice Research Institute, Cuttack, Odisha 753006 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kar", 
        "givenName": "M. K.", 
        "id": "sg:person.011536733073.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011536733073.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tamil Nadu Agricultural University, Coimbatore, 641003 India", 
          "id": "http://www.grid.ac/institutes/grid.412906.8", 
          "name": [
            "Tamil Nadu Agricultural University, Coimbatore, 641003 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Robin", 
        "givenName": "S.", 
        "id": "sg:person.01306703474.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306703474.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "INSA Honorary Scientist, NRCPB, IARI, Pusa, New Delhi, 110012 India", 
          "id": "http://www.grid.ac/institutes/grid.418196.3", 
          "name": [
            "INSA Honorary Scientist, NRCPB, IARI, Pusa, New Delhi, 110012 India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sharma", 
        "givenName": "R. P.", 
        "id": "sg:person.0641247040.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641247040.53"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1186/1471-2164-5-37", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042972117", 
          "https://doi.org/10.1186/1471-2164-5-37"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.2095", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009790366", 
          "https://doi.org/10.1038/nbt.2095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11103-004-5112-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010891397", 
          "https://doi.org/10.1007/s11103-004-5112-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00290837", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035151591", 
          "https://doi.org/10.1007/bf00290837"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-04-04", 
    "datePublishedReg": "2017-04-04", 
    "description": "BACKGROUND: Increased water and labour scarcity in major rice growing areas warrants a shift towards direct seeded rice cultivation under which management of weeds is a major issue. Use of broad spectrum non-selective herbicides is an efficient means to manage weeds. Availability of rice genotypes with complete tolerance against broad-spectrum non-selective herbicides is a pre-requisite for advocating use of such herbicides. In the present study, we developed an EMS induced rice mutant, 'HTM-N22', exhibiting tolerance to a broad spectrum herbicide, 'Imazethapyr', and identified the mutations imparting tolerance to the herbicide.\nRESULTS: We identified a stable and true breeding rice mutant, HTM-N22 (HTM), tolerant to herbicide, Imazethapyr, from an EMS-mutagenized population of approximately 100,000\u00a0M2 plants of an upland rice variety, Nagina 22 (N22). Analysis of inheritance of herbicide tolerance in a cross between Pusa 1656-10-61/HTM showed that this trait is governed by a single dominant gene. To identify the causal gene for Imazethapyr tolerance, bulked segregant analysis (BSA) was followed using microsatellite markers flanking the three putative candidate genes viz., an Acetolactate Synthase (ALS) on chromosome 6 and two Acetohydroxy Acid Synthase (AHAS) genes, one on chromosomes 2 and another on chromosome 4. RM 6844 on chromosome 2 located 0.16 Mbp upstream of AHAS (LOC_Os02g30630) was found to co-segregate with herbicide tolerance. Cloning and sequencing of AHAS (LOC_Os02g30630) from the wild type, N22 and the mutant HTM and their comparison with reference Nipponbare sequence revealed several Single Nucleotide Polymorphisms (SNPs) in the mutant, of which eight resulted in non-synonymous mutations. Three of the eight amino acid substitutions were identical to Nipponbare and hence were not considered as causal changes. Of the five putative candidate SNPs, four were novel (at positions 30, 50, 81 and 152) while the remaining one, S627D was a previously reported mutant, known to result in Imidazolinone tolerance in rice. Of the novel ones, G152E was found to alter the hydrophobicty and abolish an N myristoylation site in the HTM compared to the WT, from reference based modeling and motif prediction studies.\nCONCLUSIONS: A novel mutant tolerant to the herbicide \"Imazethapyr\" was developed and characterized for genetic, sequence and protein level variations. This is a HTM in rice without any IPR (Intellectual Property Rights) infringements and hence can be used in rice breeding as a novel genetic stock\u00a0by the public funded organizations in the country and elsewhere.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/s12284-017-0151-8", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1041344", 
        "issn": [
          "1939-8425", 
          "1939-8433"
        ], 
        "name": "Rice", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "keywords": [
      "non-selective herbicide", 
      "herbicide tolerance", 
      "acetolactate synthase", 
      "direct-seeded rice cultivation", 
      "rice mutant", 
      "acetohydroxy acid synthase genes", 
      "single nucleotide polymorphisms", 
      "management of weeds", 
      "upland rice varieties", 
      "broad-spectrum herbicide", 
      "single dominant gene", 
      "chromosome 2", 
      "imidazolinone tolerance", 
      "analysis of inheritance", 
      "Nagina 22", 
      "rice genotypes", 
      "rice breeding", 
      "major rice", 
      "rice varieties", 
      "rice cultivation", 
      "such herbicides", 
      "Nipponbare sequence", 
      "labor scarcity", 
      "segregant analysis", 
      "genetic stocks", 
      "protein level variation", 
      "Mbp upstream", 
      "dominant gene", 
      "herbicides", 
      "tolerant mutants", 
      "rice", 
      "areas warrants", 
      "imazethapyr", 
      "genes viz", 
      "non-synonymous mutations", 
      "amino acid substitutions", 
      "candidate single nucleotide polymorphisms", 
      "myristoylation site", 
      "weeds", 
      "novel mutants", 
      "causal genes", 
      "genetic characterization", 
      "microsatellite markers", 
      "synthase gene", 
      "tolerance", 
      "chromosome 4", 
      "chromosome 6", 
      "wild type", 
      "mutants", 
      "acid substitutions", 
      "AHAS", 
      "IPR infringement", 
      "genes", 
      "nucleotide polymorphisms", 
      "causal changes", 
      "breeding", 
      "mutations", 
      "Nipponbare", 
      "cultivation", 
      "N22", 
      "traits", 
      "novel ones", 
      "plants", 
      "genotypes", 
      "sequence", 
      "stocks", 
      "cross", 
      "cloning", 
      "complete tolerance", 
      "scarcity", 
      "efficient means", 
      "sequencing", 
      "availability", 
      "viz", 
      "inheritance", 
      "upstream", 
      "synthase", 
      "major issue", 
      "management", 
      "polymorphism", 
      "prediction studies", 
      "variety", 
      "water", 
      "use", 
      "present study", 
      "population", 
      "markers", 
      "variation", 
      "sites", 
      "level variations", 
      "countries", 
      "substitution", 
      "EMS", 
      "characterization", 
      "analysis", 
      "study", 
      "development", 
      "changes", 
      "shift", 
      "types", 
      "wt", 
      "organization", 
      "HTM", 
      "warrants", 
      "comparison", 
      "means", 
      "reference", 
      "one", 
      "issues", 
      "modeling", 
      "infringement", 
      "seeded rice cultivation", 
      "broad spectrum non-selective herbicides", 
      "spectrum non-selective herbicides", 
      "EMS induced rice mutant", 
      "induced rice mutant", 
      "HTM-N22", 
      "spectrum herbicide", 
      "true breeding rice mutant", 
      "breeding rice mutant", 
      "Pusa 1656", 
      "Imazethapyr tolerance", 
      "putative candidate genes viz", 
      "candidate genes viz", 
      "Acid Synthase (AHAS) genes", 
      "RM 6844", 
      "sequencing of AHAS", 
      "mutant HTM", 
      "reference Nipponbare sequence", 
      "putative candidate SNPs", 
      "S627D", 
      "G152E", 
      "hydrophobicty", 
      "motif prediction studies", 
      "novel genetic stock", 
      "Novel Herbicide (Imazethapyr) Tolerant Mutant", 
      "Herbicide (Imazethapyr) Tolerant Mutant"
    ], 
    "name": "Development and Genetic Characterization of A Novel Herbicide (Imazethapyr) Tolerant Mutant in Rice (Oryza sativa L.)", 
    "pagination": "10", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1084513443"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12284-017-0151-8"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28378144"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12284-017-0151-8", 
      "https://app.dimensions.ai/details/publication/pub.1084513443"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:43", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_719.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/s12284-017-0151-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12284-017-0151-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12284-017-0151-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12284-017-0151-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12284-017-0151-8'


 

This table displays all metadata directly associated to this object as RDF triples.

398 TRIPLES      22 PREDICATES      169 URIs      155 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12284-017-0151-8 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 anzsrc-for:07
4 anzsrc-for:0703
5 schema:author N77dbb828e9394a5c8c6fd252e5304197
6 schema:citation sg:pub.10.1007/bf00290837
7 sg:pub.10.1007/s11103-004-5112-0
8 sg:pub.10.1038/nbt.2095
9 sg:pub.10.1186/1471-2164-5-37
10 schema:datePublished 2017-04-04
11 schema:datePublishedReg 2017-04-04
12 schema:description BACKGROUND: Increased water and labour scarcity in major rice growing areas warrants a shift towards direct seeded rice cultivation under which management of weeds is a major issue. Use of broad spectrum non-selective herbicides is an efficient means to manage weeds. Availability of rice genotypes with complete tolerance against broad-spectrum non-selective herbicides is a pre-requisite for advocating use of such herbicides. In the present study, we developed an EMS induced rice mutant, 'HTM-N22', exhibiting tolerance to a broad spectrum herbicide, 'Imazethapyr', and identified the mutations imparting tolerance to the herbicide. RESULTS: We identified a stable and true breeding rice mutant, HTM-N22 (HTM), tolerant to herbicide, Imazethapyr, from an EMS-mutagenized population of approximately 100,000 M<sub>2</sub> plants of an upland rice variety, Nagina 22 (N22). Analysis of inheritance of herbicide tolerance in a cross between Pusa 1656-10-61/HTM showed that this trait is governed by a single dominant gene. To identify the causal gene for Imazethapyr tolerance, bulked segregant analysis (BSA) was followed using microsatellite markers flanking the three putative candidate genes viz., an Acetolactate Synthase (ALS) on chromosome 6 and two Acetohydroxy Acid Synthase (AHAS) genes, one on chromosomes 2 and another on chromosome 4. RM 6844 on chromosome 2 located 0.16 Mbp upstream of AHAS (LOC_Os02g30630) was found to co-segregate with herbicide tolerance. Cloning and sequencing of AHAS (LOC_Os02g30630) from the wild type, N22 and the mutant HTM and their comparison with reference Nipponbare sequence revealed several Single Nucleotide Polymorphisms (SNPs) in the mutant, of which eight resulted in non-synonymous mutations. Three of the eight amino acid substitutions were identical to Nipponbare and hence were not considered as causal changes. Of the five putative candidate SNPs, four were novel (at positions 30, 50, 81 and 152) while the remaining one, S627D was a previously reported mutant, known to result in Imidazolinone tolerance in rice. Of the novel ones, G152E was found to alter the hydrophobicty and abolish an N myristoylation site in the HTM compared to the WT, from reference based modeling and motif prediction studies. CONCLUSIONS: A novel mutant tolerant to the herbicide "Imazethapyr" was developed and characterized for genetic, sequence and protein level variations. This is a HTM in rice without any IPR (Intellectual Property Rights) infringements and hence can be used in rice breeding as a novel genetic stock by the public funded organizations in the country and elsewhere.
13 schema:genre article
14 schema:inLanguage en
15 schema:isAccessibleForFree true
16 schema:isPartOf N49a7577c8e904b88a64b74d8b7337115
17 Nd83a325329a84a54b13568140981e2f4
18 sg:journal.1041344
19 schema:keywords AHAS
20 Acid Synthase (AHAS) genes
21 EMS
22 EMS induced rice mutant
23 G152E
24 HTM
25 HTM-N22
26 Herbicide (Imazethapyr) Tolerant Mutant
27 IPR infringement
28 Imazethapyr tolerance
29 Mbp upstream
30 N22
31 Nagina 22
32 Nipponbare
33 Nipponbare sequence
34 Novel Herbicide (Imazethapyr) Tolerant Mutant
35 Pusa 1656
36 RM 6844
37 S627D
38 acetohydroxy acid synthase genes
39 acetolactate synthase
40 acid substitutions
41 amino acid substitutions
42 analysis
43 analysis of inheritance
44 areas warrants
45 availability
46 breeding
47 breeding rice mutant
48 broad spectrum non-selective herbicides
49 broad-spectrum herbicide
50 candidate genes viz
51 candidate single nucleotide polymorphisms
52 causal changes
53 causal genes
54 changes
55 characterization
56 chromosome 2
57 chromosome 4
58 chromosome 6
59 cloning
60 comparison
61 complete tolerance
62 countries
63 cross
64 cultivation
65 development
66 direct-seeded rice cultivation
67 dominant gene
68 efficient means
69 genes
70 genes viz
71 genetic characterization
72 genetic stocks
73 genotypes
74 herbicide tolerance
75 herbicides
76 hydrophobicty
77 imazethapyr
78 imidazolinone tolerance
79 induced rice mutant
80 infringement
81 inheritance
82 issues
83 labor scarcity
84 level variations
85 major issue
86 major rice
87 management
88 management of weeds
89 markers
90 means
91 microsatellite markers
92 modeling
93 motif prediction studies
94 mutant HTM
95 mutants
96 mutations
97 myristoylation site
98 non-selective herbicide
99 non-synonymous mutations
100 novel genetic stock
101 novel mutants
102 novel ones
103 nucleotide polymorphisms
104 one
105 organization
106 plants
107 polymorphism
108 population
109 prediction studies
110 present study
111 protein level variation
112 putative candidate SNPs
113 putative candidate genes viz
114 reference
115 reference Nipponbare sequence
116 rice
117 rice breeding
118 rice cultivation
119 rice genotypes
120 rice mutant
121 rice varieties
122 scarcity
123 seeded rice cultivation
124 segregant analysis
125 sequence
126 sequencing
127 sequencing of AHAS
128 shift
129 single dominant gene
130 single nucleotide polymorphisms
131 sites
132 spectrum herbicide
133 spectrum non-selective herbicides
134 stocks
135 study
136 substitution
137 such herbicides
138 synthase
139 synthase gene
140 tolerance
141 tolerant mutants
142 traits
143 true breeding rice mutant
144 types
145 upland rice varieties
146 upstream
147 use
148 variation
149 variety
150 viz
151 warrants
152 water
153 weeds
154 wild type
155 wt
156 schema:name Development and Genetic Characterization of A Novel Herbicide (Imazethapyr) Tolerant Mutant in Rice (Oryza sativa L.)
157 schema:pagination 10
158 schema:productId N1ce11f28e6bd4d3b810c0f16abe3cc0f
159 N6a66ac976a3b4c98ad4102d4c61f76aa
160 Nef48a11149214b2194aefad7efec63aa
161 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084513443
162 https://doi.org/10.1186/s12284-017-0151-8
163 schema:sdDatePublished 2022-01-01T18:43
164 schema:sdLicense https://scigraph.springernature.com/explorer/license/
165 schema:sdPublisher N635199c5de8146e8a15ba95a4bc04b1d
166 schema:url https://doi.org/10.1186/s12284-017-0151-8
167 sgo:license sg:explorer/license/
168 sgo:sdDataset articles
169 rdf:type schema:ScholarlyArticle
170 N17053a6b118b4d3caa9dabc8db61d8e1 rdf:first sg:person.01047152735.78
171 rdf:rest N9b07f157716245578cefc9402b5a0d56
172 N1b8949f79855451eaf6a39a28692b74c rdf:first sg:person.0731132341.00
173 rdf:rest N6b56fb8f96694095bdab9ee5266fdec2
174 N1ce11f28e6bd4d3b810c0f16abe3cc0f schema:name pubmed_id
175 schema:value 28378144
176 rdf:type schema:PropertyValue
177 N2ec010e59a9f4fdf9068c98f6ad2dce8 rdf:first sg:person.0641247040.53
178 rdf:rest rdf:nil
179 N34d91e66cb864aa2a1ef35ae91198794 rdf:first sg:person.014410406473.16
180 rdf:rest Nba2d60768c5b45aeb17e84acf3811576
181 N39a9d1239a2543b19b08d6f61a8352a3 rdf:first sg:person.01347002134.17
182 rdf:rest N1b8949f79855451eaf6a39a28692b74c
183 N45f7339c2d7543308189c0bb6f7e61e8 rdf:first N6de6ffc2963c4b4880581512c4f57ee7
184 rdf:rest Ncd51bc7fdc24407aae6ddf6f2c48145f
185 N49a7577c8e904b88a64b74d8b7337115 schema:issueNumber 1
186 rdf:type schema:PublicationIssue
187 N582cb47dcda6456e85fa648270160acd schema:affiliation grid-institutes:grid.412906.8
188 schema:familyName Utharasu
189 schema:givenName S.
190 rdf:type schema:Person
191 N631cfc4630f34429baad8079f5766828 rdf:first sg:person.01115266135.20
192 rdf:rest Nbe7f7e1317a44661881dc46086dd8141
193 N635199c5de8146e8a15ba95a4bc04b1d schema:name Springer Nature - SN SciGraph project
194 rdf:type schema:Organization
195 N6a66ac976a3b4c98ad4102d4c61f76aa schema:name dimensions_id
196 schema:value pub.1084513443
197 rdf:type schema:PropertyValue
198 N6b56fb8f96694095bdab9ee5266fdec2 rdf:first sg:person.01217171521.65
199 rdf:rest N92ebff6e760a41f6a27c7e753b1ea799
200 N6de6ffc2963c4b4880581512c4f57ee7 schema:affiliation grid-institutes:grid.412906.8
201 schema:familyName Ramchandar
202 schema:givenName S.
203 rdf:type schema:Person
204 N77dbb828e9394a5c8c6fd252e5304197 rdf:first sg:person.011422504473.00
205 rdf:rest Nf00e018c726140148d3be9f2184cac88
206 N8a9ac69ab13c4e948e3e75406b0583fb rdf:first sg:person.011474431111.30
207 rdf:rest N933bc11a4fbf48759c094b8571365446
208 N92ebff6e760a41f6a27c7e753b1ea799 rdf:first sg:person.014441664475.06
209 rdf:rest Nc5db82b6109848ddb7b834531f355be7
210 N933bc11a4fbf48759c094b8571365446 rdf:first N582cb47dcda6456e85fa648270160acd
211 rdf:rest N34d91e66cb864aa2a1ef35ae91198794
212 N9b07f157716245578cefc9402b5a0d56 rdf:first sg:person.013365020075.62
213 rdf:rest Nb6fb2afdce8344e2907991e617bbbbae
214 Nb6fb2afdce8344e2907991e617bbbbae rdf:first sg:person.011605605461.85
215 rdf:rest Nce1652529d2b4775b059de2a71d253c9
216 Nba2d60768c5b45aeb17e84acf3811576 rdf:first Nf1ed1c242ad14df79f0b38b5ba5ee5d0
217 rdf:rest N45f7339c2d7543308189c0bb6f7e61e8
218 Nbe7f7e1317a44661881dc46086dd8141 rdf:first sg:person.010554113672.95
219 rdf:rest N17053a6b118b4d3caa9dabc8db61d8e1
220 Nc3809cddfbfe41b4ac0970e1b34ee2a6 rdf:first sg:person.01240305735.27
221 rdf:rest N631cfc4630f34429baad8079f5766828
222 Nc5db82b6109848ddb7b834531f355be7 rdf:first sg:person.013661141273.99
223 rdf:rest Nc3809cddfbfe41b4ac0970e1b34ee2a6
224 Ncd51bc7fdc24407aae6ddf6f2c48145f rdf:first sg:person.01272001472.36
225 rdf:rest Ndbeab24db9cc44afa6106c1e923e09f5
226 Nce1652529d2b4775b059de2a71d253c9 rdf:first sg:person.011536733073.37
227 rdf:rest Nf15f280bd22a41ecb56c14cc7f4b3f6f
228 Nd83a325329a84a54b13568140981e2f4 schema:volumeNumber 10
229 rdf:type schema:PublicationVolume
230 Ndbeab24db9cc44afa6106c1e923e09f5 rdf:first sg:person.015531114055.26
231 rdf:rest N39a9d1239a2543b19b08d6f61a8352a3
232 Nef48a11149214b2194aefad7efec63aa schema:name doi
233 schema:value 10.1186/s12284-017-0151-8
234 rdf:type schema:PropertyValue
235 Nf00e018c726140148d3be9f2184cac88 rdf:first sg:person.01101441064.61
236 rdf:rest N8a9ac69ab13c4e948e3e75406b0583fb
237 Nf15f280bd22a41ecb56c14cc7f4b3f6f rdf:first sg:person.01306703474.92
238 rdf:rest N2ec010e59a9f4fdf9068c98f6ad2dce8
239 Nf1ed1c242ad14df79f0b38b5ba5ee5d0 schema:affiliation grid-institutes:grid.412906.8
240 schema:familyName Subhasini
241 schema:givenName G.
242 rdf:type schema:Person
243 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
244 schema:name Biological Sciences
245 rdf:type schema:DefinedTerm
246 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
247 schema:name Genetics
248 rdf:type schema:DefinedTerm
249 anzsrc-for:07 schema:inDefinedTermSet anzsrc-for:
250 schema:name Agricultural and Veterinary Sciences
251 rdf:type schema:DefinedTerm
252 anzsrc-for:0703 schema:inDefinedTermSet anzsrc-for:
253 schema:name Crop and Pasture Production
254 rdf:type schema:DefinedTerm
255 sg:journal.1041344 schema:issn 1939-8425
256 1939-8433
257 schema:name Rice
258 schema:publisher Springer Nature
259 rdf:type schema:Periodical
260 sg:person.01047152735.78 schema:affiliation grid-institutes:grid.466936.8
261 schema:familyName Singh
262 schema:givenName N. K.
263 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047152735.78
264 rdf:type schema:Person
265 sg:person.010554113672.95 schema:affiliation grid-institutes:grid.452695.9
266 schema:familyName Singh
267 schema:givenName Kuldeep
268 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010554113672.95
269 rdf:type schema:Person
270 sg:person.01101441064.61 schema:affiliation grid-institutes:grid.412906.8
271 schema:familyName Raveendran
272 schema:givenName M.
273 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101441064.61
274 rdf:type schema:Person
275 sg:person.01115266135.20 schema:affiliation grid-institutes:None
276 schema:familyName Mohapatra
277 schema:givenName T.
278 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115266135.20
279 rdf:type schema:Person
280 sg:person.011422504473.00 schema:affiliation grid-institutes:grid.412906.8
281 schema:familyName Shoba
282 schema:givenName D.
283 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011422504473.00
284 rdf:type schema:Person
285 sg:person.011474431111.30 schema:affiliation grid-institutes:grid.412906.8
286 schema:familyName Manonmani
287 schema:givenName S.
288 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011474431111.30
289 rdf:type schema:Person
290 sg:person.011536733073.37 schema:affiliation grid-institutes:grid.418371.8
291 schema:familyName Kar
292 schema:givenName M. K.
293 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011536733073.37
294 rdf:type schema:Person
295 sg:person.011605605461.85 schema:affiliation grid-institutes:grid.413008.e
296 schema:familyName Sheshshayee
297 schema:givenName M. S.
298 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011605605461.85
299 rdf:type schema:Person
300 sg:person.01217171521.65 schema:affiliation grid-institutes:grid.466936.8
301 schema:familyName Jayaswal
302 schema:givenName Pawan
303 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01217171521.65
304 rdf:type schema:Person
305 sg:person.01240305735.27 schema:affiliation grid-institutes:grid.466936.8
306 schema:familyName Mithra
307 schema:givenName S. V. Amitha
308 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240305735.27
309 rdf:type schema:Person
310 sg:person.01272001472.36 schema:affiliation grid-institutes:grid.412906.8
311 schema:familyName Valarmathi
312 schema:givenName R.
313 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272001472.36
314 rdf:type schema:Person
315 sg:person.01306703474.92 schema:affiliation grid-institutes:grid.412906.8
316 schema:familyName Robin
317 schema:givenName S.
318 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306703474.92
319 rdf:type schema:Person
320 sg:person.013365020075.62 schema:affiliation grid-institutes:grid.464820.c
321 schema:familyName Sarla
322 schema:givenName N.
323 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013365020075.62
324 rdf:type schema:Person
325 sg:person.01347002134.17 schema:affiliation grid-institutes:grid.418196.3
326 schema:familyName Krishnan
327 schema:givenName S. Gopala
328 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347002134.17
329 rdf:type schema:Person
330 sg:person.013661141273.99 schema:affiliation grid-institutes:grid.466936.8
331 schema:familyName Ramkumar
332 schema:givenName M. K.
333 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013661141273.99
334 rdf:type schema:Person
335 sg:person.014410406473.16 schema:affiliation grid-institutes:grid.412906.8
336 schema:familyName Dhivyapriya
337 schema:givenName D.
338 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014410406473.16
339 rdf:type schema:Person
340 sg:person.014441664475.06 schema:affiliation grid-institutes:grid.466936.8
341 schema:familyName Kale
342 schema:givenName Prashant
343 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014441664475.06
344 rdf:type schema:Person
345 sg:person.015531114055.26 schema:affiliation grid-institutes:grid.418196.3
346 schema:familyName Grover
347 schema:givenName Nitasha
348 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015531114055.26
349 rdf:type schema:Person
350 sg:person.0641247040.53 schema:affiliation grid-institutes:grid.418196.3
351 schema:familyName Sharma
352 schema:givenName R. P.
353 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641247040.53
354 rdf:type schema:Person
355 sg:person.0731132341.00 schema:affiliation grid-institutes:grid.418196.3
356 schema:familyName Singh
357 schema:givenName A. K.
358 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731132341.00
359 rdf:type schema:Person
360 sg:pub.10.1007/bf00290837 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035151591
361 https://doi.org/10.1007/bf00290837
362 rdf:type schema:CreativeWork
363 sg:pub.10.1007/s11103-004-5112-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010891397
364 https://doi.org/10.1007/s11103-004-5112-0
365 rdf:type schema:CreativeWork
366 sg:pub.10.1038/nbt.2095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009790366
367 https://doi.org/10.1038/nbt.2095
368 rdf:type schema:CreativeWork
369 sg:pub.10.1186/1471-2164-5-37 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042972117
370 https://doi.org/10.1186/1471-2164-5-37
371 rdf:type schema:CreativeWork
372 grid-institutes:None schema:alternateName Indian Council of Agriculture Research, New Delhi, 110 001 India
373 schema:name Indian Council of Agriculture Research, New Delhi, 110 001 India
374 rdf:type schema:Organization
375 grid-institutes:grid.412906.8 schema:alternateName Tamil Nadu Agricultural University, Coimbatore, 641003 India
376 schema:name Tamil Nadu Agricultural University, Coimbatore, 641003 India
377 rdf:type schema:Organization
378 grid-institutes:grid.413008.e schema:alternateName University of Agricultural Sciences, Bengaluru, 560065 India
379 schema:name University of Agricultural Sciences, Bengaluru, 560065 India
380 rdf:type schema:Organization
381 grid-institutes:grid.418196.3 schema:alternateName Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India
382 INSA Honorary Scientist, NRCPB, IARI, Pusa, New Delhi, 110012 India
383 schema:name Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India
384 INSA Honorary Scientist, NRCPB, IARI, Pusa, New Delhi, 110012 India
385 rdf:type schema:Organization
386 grid-institutes:grid.418371.8 schema:alternateName National Rice Research Institute, Cuttack, Odisha 753006 India
387 schema:name National Rice Research Institute, Cuttack, Odisha 753006 India
388 rdf:type schema:Organization
389 grid-institutes:grid.452695.9 schema:alternateName Present address: ICAR-National Bureau of Plant Genetic Resources, Pusa, New Delhi, 110012 India
390 schema:name Present address: ICAR-National Bureau of Plant Genetic Resources, Pusa, New Delhi, 110012 India
391 Punjab Agricultural University, Ludhiana, 141004 India
392 rdf:type schema:Organization
393 grid-institutes:grid.464820.c schema:alternateName Indian Institute of Rice Research, Rajendranagar, Hyderabad, 500030 India
394 schema:name Indian Institute of Rice Research, Rajendranagar, Hyderabad, 500030 India
395 rdf:type schema:Organization
396 grid-institutes:grid.466936.8 schema:alternateName ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi, 110012 India
397 schema:name ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi, 110012 India
398 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...