Fullerene C60 Penetration into Leukemic Cells and Its Photoinduced Cytotoxic Effects View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

D. Franskevych, K. Palyvoda, D. Petukhov, S. Prylutska, I. Grynyuk, C. Schuetze, L. Drobot, O. Matyshevska, U. Ritter

ABSTRACT

Fullerene C60 as a representative of carbon nanocompounds is suggested to be promising agent for application in photodynamic therapy due to its unique physicochemical properties. The goal of this study was to estimate the accumulation of fullerene C60 in leukemic cells and to investigate its phototoxic effect on parental and resistant to cisplatin leukemic cells. Stable homogeneous water colloid solution of pristine C60 with average 50-nm diameter of nanoparticles was used in experiments. Fluorescent labeled C60 was synthesized by covalent conjugation of C60 with rhodamine B isothiocyanate. The results of confocal microscopy showed that leukemic Jurkat cells could effectively uptake fullerene C60 from the medium. Light-emitting diode lamp (100 mW cm-2, λ = 420-700 nm) was used for excitation of accumulated C60. A time-dependent decrease of viability was detected when leukemic Jurkat cells were exposed to combined treatment with C60 and visible light. The cytotoxic effect of photoexcited C60 was comparable with that induced by H2O2, as both agents caused 50% decrease of cell viability at 24 h at concentrations about 50 μM. Using immunoblot analysis, protein phosphotyrosine levels in cells were estimated. Combined action of C60 and visible light was followed by decrease of cellular proteins phosphorylation on tyrosine residues though less intensive as compared with that induced by H2O2 or protein tyrosine kinase inhibitor staurosporine. All tested agents reduced phosphorylation of 55, 70, and 90 kDa proteins while total suppression of 26 kDa protein phosphorylation was specific only for photoexcited C60.The cytotoxic effect of C60 in combination with visible light irradiation was demonstrated also on leukemic L1210 cells both sensitive and resistant to cisplatin. It was shown that relative value of mitochondrial membrane potential measured with tetramethylrhodamine ethyl ester perchlorate (TMRE) probe was lower in resistant cells in comparison with sensitive cells and the drop of mitochondrial potential corresponded to further decrease of resistant cell viability after C60 photoexcitation. The data obtained allow to suggest that C60-mediated photodynamic treatment is a candidate for restoration of drug-resistant leukemic cell sensitivity to induction of mitochondrial way of apoptosis. More... »

PAGES

40

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s11671-016-1819-5

DOI

http://dx.doi.org/10.1186/s11671-016-1819-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023566555

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28091953


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Taras Shevchenko National University of Kyiv", 
          "id": "https://www.grid.ac/institutes/grid.34555.32", 
          "name": [
            "National Taras Shevchenko University of Kyiv, 64/13 Volodymyrska Street, 01601, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Franskevych", 
        "givenName": "D.", 
        "id": "sg:person.01272701061.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272701061.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Taras Shevchenko National University of Kyiv", 
          "id": "https://www.grid.ac/institutes/grid.34555.32", 
          "name": [
            "National Taras Shevchenko University of Kyiv, 64/13 Volodymyrska Street, 01601, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Palyvoda", 
        "givenName": "K.", 
        "id": "sg:person.01267003642.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01267003642.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Palladin Institute of Biochemistry", 
          "id": "https://www.grid.ac/institutes/grid.419966.5", 
          "name": [
            "Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 9 Leontovicha Street, 01030, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Petukhov", 
        "givenName": "D.", 
        "id": "sg:person.016462647251.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016462647251.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Taras Shevchenko National University of Kyiv", 
          "id": "https://www.grid.ac/institutes/grid.34555.32", 
          "name": [
            "National Taras Shevchenko University of Kyiv, 64/13 Volodymyrska Street, 01601, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Prylutska", 
        "givenName": "S.", 
        "id": "sg:person.01265014537.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265014537.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Taras Shevchenko National University of Kyiv", 
          "id": "https://www.grid.ac/institutes/grid.34555.32", 
          "name": [
            "National Taras Shevchenko University of Kyiv, 64/13 Volodymyrska Street, 01601, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Grynyuk", 
        "givenName": "I.", 
        "id": "sg:person.01042167742.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042167742.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ilmenau University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.6553.5", 
          "name": [
            "Ilmenau University of Technology, 29 Ehrenbergstrasse, 98693, Ilmenau, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schuetze", 
        "givenName": "C.", 
        "id": "sg:person.016275322123.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016275322123.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Palladin Institute of Biochemistry", 
          "id": "https://www.grid.ac/institutes/grid.419966.5", 
          "name": [
            "Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 9 Leontovicha Street, 01030, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Drobot", 
        "givenName": "L.", 
        "id": "sg:person.01325113314.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01325113314.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Taras Shevchenko National University of Kyiv", 
          "id": "https://www.grid.ac/institutes/grid.34555.32", 
          "name": [
            "National Taras Shevchenko University of Kyiv, 64/13 Volodymyrska Street, 01601, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matyshevska", 
        "givenName": "O.", 
        "id": "sg:person.01037270327.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037270327.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ilmenau University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.6553.5", 
          "name": [
            "Ilmenau University of Technology, 29 Ehrenbergstrasse, 98693, Ilmenau, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ritter", 
        "givenName": "U.", 
        "id": "sg:person.0664526675.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664526675.56"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.msec.2011.02.026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003595989"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmcc.1997.0614", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004815866"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/pr.111.005637", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006476564"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0300-483x(00)00277-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006608708"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1349-7006.1997.tb00336.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006749290"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1349-7006.1997.tb00336.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006749290"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cbi.2011.11.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008429937"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.progpolymsci.2004.08.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009913583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.nano.2013.11.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015434811"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11010-014-1968-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018646485", 
          "https://doi.org/10.1007/s11010-014-1968-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/mawe.201300105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022310712"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1011-1344(02)00320-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022839571"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m301546200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022892664"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c2sm06811g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023445754"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nnano.2008.130", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024119561", 
          "https://doi.org/10.1038/nnano.2008.130"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.freeradbiomed.2010.05.031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024866287"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jps.23458", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026573990"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1477-3155-4-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032470725", 
          "https://doi.org/10.1186/1477-3155-4-14"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0909707107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033630097"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1166/jbn.2014.1963", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035492014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.freeradbiomed.2007.05.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036260810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3727/096504011x12970940207805", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036304713"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.carbon.2003.12.053", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040008358"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-3495(99)77214-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040951256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00280-003-0726-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047060975", 
          "https://doi.org/10.1007/s00280-003-0726-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c5nr07003a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047924515"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/toxsci/kft327", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051513572"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acs.est.5b00100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055087369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/es702905g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055514526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/es702905g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055514526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00074a056", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055704532"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jm3000664", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055952866"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jb/mvq068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059746290"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jb/mvq068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059746290"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpgi.00467.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063193264"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.15407/biotech6.06.071", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067791123"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4236/jct.2016.73024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072385779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076347700", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/030089160809400221", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077663475"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/030089160809400221", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077663475"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078386695", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078605291", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079959685", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/025197", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085104597"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/025197", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085104597"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/025197", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085104597"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-12", 
    "datePublishedReg": "2017-12-01", 
    "description": "Fullerene C60 as a representative of carbon nanocompounds is suggested to be promising agent for application in photodynamic therapy due to its unique physicochemical properties. The goal of this study was to estimate the accumulation of fullerene C60 in leukemic cells and to investigate its phototoxic effect on parental and resistant to cisplatin leukemic cells. Stable homogeneous water colloid solution of pristine C60 with average 50-nm diameter of nanoparticles was used in experiments. Fluorescent labeled C60 was synthesized by covalent conjugation of C60 with rhodamine B isothiocyanate. The results of confocal microscopy showed that leukemic Jurkat cells could effectively uptake fullerene C60 from the medium. Light-emitting diode lamp (100\u00a0mW\u00a0cm-2, \u03bb\u2009=\u2009420-700\u00a0nm) was used for excitation of accumulated C60. A time-dependent decrease of viability was detected when leukemic Jurkat cells were exposed to combined treatment with C60 and visible light. The cytotoxic effect of photoexcited C60 was comparable with that induced by H2O2, as both agents caused 50% decrease of cell viability at 24\u00a0h at concentrations about 50\u00a0\u03bcM. Using immunoblot analysis, protein phosphotyrosine levels in cells were estimated. Combined action of C60 and visible light was followed by decrease of cellular proteins phosphorylation on tyrosine residues though less intensive as compared with that induced by H2O2 or protein tyrosine kinase inhibitor staurosporine. All tested agents reduced phosphorylation of 55, 70, and 90\u00a0kDa proteins while total suppression of 26\u00a0kDa protein phosphorylation was specific only for photoexcited C60.The cytotoxic effect of C60 in combination with visible light irradiation was demonstrated also on leukemic L1210 cells both sensitive and resistant to cisplatin. It was shown that relative value of mitochondrial membrane potential measured with tetramethylrhodamine ethyl ester perchlorate (TMRE) probe was lower in resistant cells in comparison with sensitive cells and the drop of mitochondrial potential corresponded to further decrease of resistant cell viability after C60 photoexcitation. The data obtained allow to suggest that C60-mediated photodynamic treatment is a candidate for restoration of drug-resistant leukemic cell sensitivity to induction of mitochondrial way of apoptosis.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s11671-016-1819-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1037280", 
        "issn": [
          "1931-7573", 
          "1556-276X"
        ], 
        "name": "Nanoscale Research Letters", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "12"
      }
    ], 
    "name": "Fullerene C60 Penetration into Leukemic Cells and Its Photoinduced Cytotoxic Effects", 
    "pagination": "40", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "70473dc4f9fda2994fb41294dd300582c92980e79083e56ca4c97fe73b94b717"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28091953"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101279750"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s11671-016-1819-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1023566555"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s11671-016-1819-5", 
      "https://app.dimensions.ai/details/publication/pub.1023566555"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:35", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000346_0000000346/records_99821_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs11671-016-1819-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s11671-016-1819-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s11671-016-1819-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s11671-016-1819-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s11671-016-1819-5'


 

This table displays all metadata directly associated to this object as RDF triples.

251 TRIPLES      21 PREDICATES      69 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s11671-016-1819-5 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N007a16cda16c4fee8ca8eccd0cff8f15
4 schema:citation sg:pub.10.1007/s00280-003-0726-5
5 sg:pub.10.1007/s11010-014-1968-8
6 sg:pub.10.1038/nnano.2008.130
7 sg:pub.10.1186/1477-3155-4-14
8 https://app.dimensions.ai/details/publication/pub.1076347700
9 https://app.dimensions.ai/details/publication/pub.1078386695
10 https://app.dimensions.ai/details/publication/pub.1078605291
11 https://app.dimensions.ai/details/publication/pub.1079959685
12 https://doi.org/10.1002/jps.23458
13 https://doi.org/10.1002/mawe.201300105
14 https://doi.org/10.1006/jmcc.1997.0614
15 https://doi.org/10.1016/j.carbon.2003.12.053
16 https://doi.org/10.1016/j.cbi.2011.11.003
17 https://doi.org/10.1016/j.freeradbiomed.2007.05.005
18 https://doi.org/10.1016/j.freeradbiomed.2010.05.031
19 https://doi.org/10.1016/j.msec.2011.02.026
20 https://doi.org/10.1016/j.nano.2013.11.014
21 https://doi.org/10.1016/j.progpolymsci.2004.08.001
22 https://doi.org/10.1016/s0006-3495(99)77214-0
23 https://doi.org/10.1016/s0300-483x(00)00277-8
24 https://doi.org/10.1016/s1011-1344(02)00320-2
25 https://doi.org/10.1021/acs.est.5b00100
26 https://doi.org/10.1021/es702905g
27 https://doi.org/10.1021/ja00074a056
28 https://doi.org/10.1021/jm3000664
29 https://doi.org/10.1039/c2sm06811g
30 https://doi.org/10.1039/c5nr07003a
31 https://doi.org/10.1073/pnas.0909707107
32 https://doi.org/10.1074/jbc.m301546200
33 https://doi.org/10.1093/jb/mvq068
34 https://doi.org/10.1093/toxsci/kft327
35 https://doi.org/10.1101/025197
36 https://doi.org/10.1111/j.1349-7006.1997.tb00336.x
37 https://doi.org/10.1124/pr.111.005637
38 https://doi.org/10.1152/ajpgi.00467.2001
39 https://doi.org/10.1166/jbn.2014.1963
40 https://doi.org/10.1177/030089160809400221
41 https://doi.org/10.15407/biotech6.06.071
42 https://doi.org/10.3727/096504011x12970940207805
43 https://doi.org/10.4236/jct.2016.73024
44 schema:datePublished 2017-12
45 schema:datePublishedReg 2017-12-01
46 schema:description Fullerene C<sub>60</sub> as a representative of carbon nanocompounds is suggested to be promising agent for application in photodynamic therapy due to its unique physicochemical properties. The goal of this study was to estimate the accumulation of fullerene C<sub>60</sub> in leukemic cells and to investigate its phototoxic effect on parental and resistant to cisplatin leukemic cells. Stable homogeneous water colloid solution of pristine C<sub>60</sub> with average 50-nm diameter of nanoparticles was used in experiments. Fluorescent labeled C<sub>60</sub> was synthesized by covalent conjugation of C<sub>60</sub> with rhodamine B isothiocyanate. The results of confocal microscopy showed that leukemic Jurkat cells could effectively uptake fullerene C<sub>60</sub> from the medium. Light-emitting diode lamp (100 mW cm<sup>-2</sup>, λ = 420-700 nm) was used for excitation of accumulated C<sub>60</sub>. A time-dependent decrease of viability was detected when leukemic Jurkat cells were exposed to combined treatment with C<sub>60</sub> and visible light. The cytotoxic effect of photoexcited C<sub>60</sub> was comparable with that induced by H<sub>2</sub>O<sub>2</sub>, as both agents caused 50% decrease of cell viability at 24 h at concentrations about 50 μM. Using immunoblot analysis, protein phosphotyrosine levels in cells were estimated. Combined action of C<sub>60</sub> and visible light was followed by decrease of cellular proteins phosphorylation on tyrosine residues though less intensive as compared with that induced by H<sub>2</sub>O<sub>2</sub> or protein tyrosine kinase inhibitor staurosporine. All tested agents reduced phosphorylation of 55, 70, and 90 kDa proteins while total suppression of 26 kDa protein phosphorylation was specific only for photoexcited C<sub>60</sub>.The cytotoxic effect of C<sub>60</sub> in combination with visible light irradiation was demonstrated also on leukemic L1210 cells both sensitive and resistant to cisplatin. It was shown that relative value of mitochondrial membrane potential measured with tetramethylrhodamine ethyl ester perchlorate (TMRE) probe was lower in resistant cells in comparison with sensitive cells and the drop of mitochondrial potential corresponded to further decrease of resistant cell viability after C<sub>60</sub> photoexcitation. The data obtained allow to suggest that C<sub>60</sub>-mediated photodynamic treatment is a candidate for restoration of drug-resistant leukemic cell sensitivity to induction of mitochondrial way of apoptosis.
47 schema:genre research_article
48 schema:inLanguage en
49 schema:isAccessibleForFree true
50 schema:isPartOf N07734227408d40fcadc5856612f45b4a
51 N74c282985e564d8cb0a0eec98d2885ed
52 sg:journal.1037280
53 schema:name Fullerene C60 Penetration into Leukemic Cells and Its Photoinduced Cytotoxic Effects
54 schema:pagination 40
55 schema:productId N2fce7acc81124e19956fc457e0d6302e
56 N6dfa012fc2dc4c8789fb34a1c74c4fa6
57 N6f3d3e8ae3cd4f0a9acb26b20eaeaa08
58 N83103a111d994aba8b0ec13f7eaa025d
59 Nee7c71e96d3e42b7944971d7ce76d3a2
60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023566555
61 https://doi.org/10.1186/s11671-016-1819-5
62 schema:sdDatePublished 2019-04-11T09:35
63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
64 schema:sdPublisher Ne325cbcee8d54317a92f66874e85b0fd
65 schema:url https://link.springer.com/10.1186%2Fs11671-016-1819-5
66 sgo:license sg:explorer/license/
67 sgo:sdDataset articles
68 rdf:type schema:ScholarlyArticle
69 N007a16cda16c4fee8ca8eccd0cff8f15 rdf:first sg:person.01272701061.38
70 rdf:rest N91862e48bf6e4e1ab408062fc0e8727e
71 N07734227408d40fcadc5856612f45b4a schema:issueNumber 1
72 rdf:type schema:PublicationIssue
73 N0c562aebb9794858ad868c971978dc9a rdf:first sg:person.01325113314.62
74 rdf:rest Ne56ffcc302d14e0cb5f92a3b3a525b84
75 N0ed168bc79b2462e8295fedb80e27fbe rdf:first sg:person.01265014537.58
76 rdf:rest Nb78b2bd0f2ff495db24ce70ed654586b
77 N1b7149ec3aab48d29667d69a7da6131b rdf:first sg:person.016462647251.02
78 rdf:rest N0ed168bc79b2462e8295fedb80e27fbe
79 N2fce7acc81124e19956fc457e0d6302e schema:name dimensions_id
80 schema:value pub.1023566555
81 rdf:type schema:PropertyValue
82 N6dfa012fc2dc4c8789fb34a1c74c4fa6 schema:name pubmed_id
83 schema:value 28091953
84 rdf:type schema:PropertyValue
85 N6f3d3e8ae3cd4f0a9acb26b20eaeaa08 schema:name doi
86 schema:value 10.1186/s11671-016-1819-5
87 rdf:type schema:PropertyValue
88 N74c282985e564d8cb0a0eec98d2885ed schema:volumeNumber 12
89 rdf:type schema:PublicationVolume
90 N83103a111d994aba8b0ec13f7eaa025d schema:name readcube_id
91 schema:value 70473dc4f9fda2994fb41294dd300582c92980e79083e56ca4c97fe73b94b717
92 rdf:type schema:PropertyValue
93 N91862e48bf6e4e1ab408062fc0e8727e rdf:first sg:person.01267003642.50
94 rdf:rest N1b7149ec3aab48d29667d69a7da6131b
95 Nb78b2bd0f2ff495db24ce70ed654586b rdf:first sg:person.01042167742.25
96 rdf:rest Nc191f84acfc642b0b73f733412763f06
97 Nbe95444cd54c444b9762b6871374a797 rdf:first sg:person.0664526675.56
98 rdf:rest rdf:nil
99 Nc191f84acfc642b0b73f733412763f06 rdf:first sg:person.016275322123.11
100 rdf:rest N0c562aebb9794858ad868c971978dc9a
101 Ne325cbcee8d54317a92f66874e85b0fd schema:name Springer Nature - SN SciGraph project
102 rdf:type schema:Organization
103 Ne56ffcc302d14e0cb5f92a3b3a525b84 rdf:first sg:person.01037270327.44
104 rdf:rest Nbe95444cd54c444b9762b6871374a797
105 Nee7c71e96d3e42b7944971d7ce76d3a2 schema:name nlm_unique_id
106 schema:value 101279750
107 rdf:type schema:PropertyValue
108 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
109 schema:name Biological Sciences
110 rdf:type schema:DefinedTerm
111 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
112 schema:name Biochemistry and Cell Biology
113 rdf:type schema:DefinedTerm
114 sg:journal.1037280 schema:issn 1556-276X
115 1931-7573
116 schema:name Nanoscale Research Letters
117 rdf:type schema:Periodical
118 sg:person.01037270327.44 schema:affiliation https://www.grid.ac/institutes/grid.34555.32
119 schema:familyName Matyshevska
120 schema:givenName O.
121 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037270327.44
122 rdf:type schema:Person
123 sg:person.01042167742.25 schema:affiliation https://www.grid.ac/institutes/grid.34555.32
124 schema:familyName Grynyuk
125 schema:givenName I.
126 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042167742.25
127 rdf:type schema:Person
128 sg:person.01265014537.58 schema:affiliation https://www.grid.ac/institutes/grid.34555.32
129 schema:familyName Prylutska
130 schema:givenName S.
131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265014537.58
132 rdf:type schema:Person
133 sg:person.01267003642.50 schema:affiliation https://www.grid.ac/institutes/grid.34555.32
134 schema:familyName Palyvoda
135 schema:givenName K.
136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01267003642.50
137 rdf:type schema:Person
138 sg:person.01272701061.38 schema:affiliation https://www.grid.ac/institutes/grid.34555.32
139 schema:familyName Franskevych
140 schema:givenName D.
141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272701061.38
142 rdf:type schema:Person
143 sg:person.01325113314.62 schema:affiliation https://www.grid.ac/institutes/grid.419966.5
144 schema:familyName Drobot
145 schema:givenName L.
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01325113314.62
147 rdf:type schema:Person
148 sg:person.016275322123.11 schema:affiliation https://www.grid.ac/institutes/grid.6553.5
149 schema:familyName Schuetze
150 schema:givenName C.
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016275322123.11
152 rdf:type schema:Person
153 sg:person.016462647251.02 schema:affiliation https://www.grid.ac/institutes/grid.419966.5
154 schema:familyName Petukhov
155 schema:givenName D.
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016462647251.02
157 rdf:type schema:Person
158 sg:person.0664526675.56 schema:affiliation https://www.grid.ac/institutes/grid.6553.5
159 schema:familyName Ritter
160 schema:givenName U.
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664526675.56
162 rdf:type schema:Person
163 sg:pub.10.1007/s00280-003-0726-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047060975
164 https://doi.org/10.1007/s00280-003-0726-5
165 rdf:type schema:CreativeWork
166 sg:pub.10.1007/s11010-014-1968-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018646485
167 https://doi.org/10.1007/s11010-014-1968-8
168 rdf:type schema:CreativeWork
169 sg:pub.10.1038/nnano.2008.130 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024119561
170 https://doi.org/10.1038/nnano.2008.130
171 rdf:type schema:CreativeWork
172 sg:pub.10.1186/1477-3155-4-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032470725
173 https://doi.org/10.1186/1477-3155-4-14
174 rdf:type schema:CreativeWork
175 https://app.dimensions.ai/details/publication/pub.1076347700 schema:CreativeWork
176 https://app.dimensions.ai/details/publication/pub.1078386695 schema:CreativeWork
177 https://app.dimensions.ai/details/publication/pub.1078605291 schema:CreativeWork
178 https://app.dimensions.ai/details/publication/pub.1079959685 schema:CreativeWork
179 https://doi.org/10.1002/jps.23458 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026573990
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1002/mawe.201300105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022310712
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1006/jmcc.1997.0614 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004815866
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/j.carbon.2003.12.053 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040008358
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/j.cbi.2011.11.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008429937
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/j.freeradbiomed.2007.05.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036260810
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/j.freeradbiomed.2010.05.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024866287
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/j.msec.2011.02.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003595989
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/j.nano.2013.11.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015434811
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/j.progpolymsci.2004.08.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009913583
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/s0006-3495(99)77214-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040951256
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/s0300-483x(00)00277-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006608708
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/s1011-1344(02)00320-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022839571
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1021/acs.est.5b00100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055087369
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1021/es702905g schema:sameAs https://app.dimensions.ai/details/publication/pub.1055514526
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1021/ja00074a056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055704532
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1021/jm3000664 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055952866
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1039/c2sm06811g schema:sameAs https://app.dimensions.ai/details/publication/pub.1023445754
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1039/c5nr07003a schema:sameAs https://app.dimensions.ai/details/publication/pub.1047924515
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1073/pnas.0909707107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033630097
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1074/jbc.m301546200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022892664
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1093/jb/mvq068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059746290
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1093/toxsci/kft327 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051513572
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1101/025197 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085104597
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1111/j.1349-7006.1997.tb00336.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006749290
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1124/pr.111.005637 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006476564
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1152/ajpgi.00467.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063193264
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1166/jbn.2014.1963 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035492014
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1177/030089160809400221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077663475
236 rdf:type schema:CreativeWork
237 https://doi.org/10.15407/biotech6.06.071 schema:sameAs https://app.dimensions.ai/details/publication/pub.1067791123
238 rdf:type schema:CreativeWork
239 https://doi.org/10.3727/096504011x12970940207805 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036304713
240 rdf:type schema:CreativeWork
241 https://doi.org/10.4236/jct.2016.73024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072385779
242 rdf:type schema:CreativeWork
243 https://www.grid.ac/institutes/grid.34555.32 schema:alternateName Taras Shevchenko National University of Kyiv
244 schema:name National Taras Shevchenko University of Kyiv, 64/13 Volodymyrska Street, 01601, Kyiv, Ukraine
245 rdf:type schema:Organization
246 https://www.grid.ac/institutes/grid.419966.5 schema:alternateName Palladin Institute of Biochemistry
247 schema:name Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 9 Leontovicha Street, 01030, Kyiv, Ukraine
248 rdf:type schema:Organization
249 https://www.grid.ac/institutes/grid.6553.5 schema:alternateName Ilmenau University of Technology
250 schema:name Ilmenau University of Technology, 29 Ehrenbergstrasse, 98693, Ilmenau, Germany
251 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...