Increased H3K4me3 methylation and decreased miR-7113-5p expression lead to enhanced Wnt/β-catenin signaling in immune cells from PTSD patients leading to ... View Full Text


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Article Info

DATE

2020-11-14

AUTHORS

Marpe Bam, Xiaoming Yang, Brandon P. Busbee, Allison E. Aiello, Monica Uddin, Jay P. Ginsberg, Sandro Galea, Prakash S. Nagarkatti, Mitzi Nagarkatti

ABSTRACT

BackgroundPosttraumatic stress disorder (PTSD) is a psychiatric disorder accompanied by chronic peripheral inflammation. What triggers inflammation in PTSD is currently unclear. In the present study, we identified potential defects in signaling pathways in peripheral blood mononuclear cells (PBMCs) from individuals with PTSD.MethodsRNAseq (5 samples each for controls and PTSD), ChIPseq (5 samples each) and miRNA array (6 samples each) were used in combination with bioinformatics tools to identify dysregulated genes in PBMCs. Real time qRT-PCR (24 samples each) and in vitro assays were employed to validate our primary findings and hypothesis.ResultsBy RNA-seq analysis of PBMCs, we found that Wnt signaling pathway was upregulated in PTSD when compared to normal controls. Specifically, we found increased expression of WNT10B in the PTSD group when compared to controls. Our findings were confirmed using NCBI’s GEO database involving a larger sample size. Additionally, in vitro activation studies revealed that activated but not naïve PBMCs from control individuals expressed more IFNγ in the presence of recombinant WNT10B suggesting that Wnt signaling played a crucial role in exacerbating inflammation. Next, we investigated the mechanism of induction of WNT10B and found that increased expression of WNT10B may result from epigenetic modulation involving downregulation of hsa-miR-7113-5p which targeted WNT10B. Furthermore, we also observed that WNT10B overexpression was linked to higher expression of H3K4me3 histone modification around the promotor of WNT10B. Additionally, knockdown of histone demethylase specific to H3K4me3, using siRNA, led to increased expression of WNT10B providing conclusive evidence that H3K4me3 indeed controlled WNT10B expression.ConclusionsIn summary, our data demonstrate for the first time that Wnt signaling pathway is upregulated in PBMCs of PTSD patients resulting from epigenetic changes involving microRNA dysregulation and histone modifications, which in turn may promote the inflammatory phenotype in such cells. More... »

PAGES

110

References to SciGraph publications

  • 2006-05-21. A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling in NATURE
  • 2005-01. Wnt signaling in disease and in development in CELL RESEARCH
  • 2010-05-02. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation in NATURE BIOTECHNOLOGY
  • 2011-10-22. Evaluation of Histone-Modifying Enzymes in Stem Cell Populations in TRANSCRIPTIONAL REGULATION
  • 2013-04-25. TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions in GENOME BIOLOGY
  • 2005-02-01. Peripheral blood mononuclear cell gene expression profiles identify emergent post-traumatic stress disorder among trauma survivors in MOLECULAR PSYCHIATRY
  • 2014-11-18. Neural stem cells in Parkinson’s disease: a role for neurogenesis defects in onset and progression in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 2009-01-04. Small molecule–mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer in NATURE CHEMICAL BIOLOGY
  • 2013-01-29. Posttraumatic stress disorder is associated with an enhanced spontaneous production of pro-inflammatory cytokines by peripheral blood mononuclear cells in BMC PSYCHIATRY
  • 2012-11-01. Wnt/β-catenin and its Diverse Physiological Cell Signaling Pathways in Neurodegenerative and Neuropsychiatric Disorders in JOURNAL OF NEUROIMMUNE PHARMACOLOGY
  • 2016-08-11. Dysregulated immune system networks in war veterans with PTSD is an outcome of altered miRNA expression and DNA methylation in SCIENTIFIC REPORTS
  • 2017-08-29. Decreased AGO2 and DCR1 in PBMCs from War Veterans with PTSD leads to diminished miRNA resulting in elevated inflammation in TRANSLATIONAL PSYCHIATRY
  • 2015-11-20. Evidence for Epigenetic Regulation of Pro-Inflammatory Cytokines, Interleukin-12 and Interferon Gamma, in Peripheral Blood Mononuclear Cells from PTSD Patients in JOURNAL OF NEUROIMMUNE PHARMACOLOGY
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    http://scigraph.springernature.com/pub.10.1186/s10020-020-00238-3

    DOI

    http://dx.doi.org/10.1186/s10020-020-00238-3

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    https://app.dimensions.ai/details/publication/pub.1132645816

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/33189141


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