Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-02

AUTHORS

Daniel Aird, Michael G Ross, Wei-Sheng Chen, Maxwell Danielsson, Timothy Fennell, Carsten Russ, David B Jaffe, Chad Nusbaum, Andreas Gnirke

ABSTRACT

Despite the ever-increasing output of Illumina sequencing data, loci with extreme base compositions are often under-represented or absent. To evaluate sources of base-composition bias, we traced genomic sequences ranging from 6% to 90% GC through the process by quantitative PCR. We identified PCR during library preparation as a principal source of bias and optimized the conditions. Our improved protocol significantly reduces amplification bias and minimizes the previously severe effects of PCR instrument and temperature ramp rate. More... »

PAGES

r18

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/gb-2011-12-2-r18

DOI

http://dx.doi.org/10.1186/gb-2011-12-2-r18

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000268052

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21338519


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Artifacts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Composition", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Loci", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genome, Human", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genomic Library", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genomics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleic Acid Denaturation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmodium falciparum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Real-Time Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Broad Institute", 
          "id": "https://www.grid.ac/institutes/grid.66859.34", 
          "name": [
            "Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 320 Charles Street, 02141, Cambridge, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Aird", 
        "givenName": "Daniel", 
        "id": "sg:person.01064510456.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064510456.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Broad Institute", 
          "id": "https://www.grid.ac/institutes/grid.66859.34", 
          "name": [
            "Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 320 Charles Street, 02141, Cambridge, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ross", 
        "givenName": "Michael G", 
        "id": "sg:person.01235503516.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235503516.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Learning Community C, Cambridge Rindge and Latin School, 459 Broadway, 02138, Cambridge, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Wei-Sheng", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Learning Community C, Cambridge Rindge and Latin School, 459 Broadway, 02138, Cambridge, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Danielsson", 
        "givenName": "Maxwell", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Broad Institute", 
          "id": "https://www.grid.ac/institutes/grid.66859.34", 
          "name": [
            "Genome Sequencing Platform, Broad Institute of MIT and Harvard, 320 Charles Street, 02141, Cambridge, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fennell", 
        "givenName": "Timothy", 
        "id": "sg:person.016012720777.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016012720777.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Broad Institute", 
          "id": "https://www.grid.ac/institutes/grid.66859.34", 
          "name": [
            "Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 320 Charles Street, 02141, Cambridge, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Russ", 
        "givenName": "Carsten", 
        "id": "sg:person.01300612454.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300612454.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Broad Institute", 
          "id": "https://www.grid.ac/institutes/grid.66859.34", 
          "name": [
            "Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 320 Charles Street, 02141, Cambridge, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jaffe", 
        "givenName": "David B", 
        "id": "sg:person.01355660531.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355660531.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Broad Institute", 
          "id": "https://www.grid.ac/institutes/grid.66859.34", 
          "name": [
            "Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 320 Charles Street, 02141, Cambridge, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nusbaum", 
        "givenName": "Chad", 
        "id": "sg:person.01170225154.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170225154.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Broad Institute", 
          "id": "https://www.grid.ac/institutes/grid.66859.34", 
          "name": [
            "Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 320 Charles Street, 02141, Cambridge, MA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gnirke", 
        "givenName": "Andreas", 
        "id": "sg:person.0645200471.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645200471.91"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nmeth.1179", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001773338", 
          "https://doi.org/10.1038/nmeth.1179"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1507", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005850821", 
          "https://doi.org/10.1038/nmeth.1507"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1561", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008087076", 
          "https://doi.org/10.1038/nbt.1561"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1561", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008087076", 
          "https://doi.org/10.1038/nbt.1561"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1561", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008087076", 
          "https://doi.org/10.1038/nbt.1561"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2009-10-10-r103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012804464", 
          "https://doi.org/10.1186/gb-2009-10-10-r103"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1181498", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014947319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1181498", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014947319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.091868.109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017676111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2009-10-6-r60", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019838301", 
          "https://doi.org/10.1186/gb-2009-10-6-r60"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1162986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021160342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1270", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021189255", 
          "https://doi.org/10.1038/nmeth.1270"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03959", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021574562", 
          "https://doi.org/10.1038/nature03959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03959", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021574562", 
          "https://doi.org/10.1038/nature03959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1311", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023653905", 
          "https://doi.org/10.1038/nmeth.1311"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1276", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025333243", 
          "https://doi.org/10.1038/nmeth.1276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.1929904", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027815825"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01097", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030736656", 
          "https://doi.org/10.1038/nature01097"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01097", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030736656", 
          "https://doi.org/10.1038/nature01097"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0007778", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033210750"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btp324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038266369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03481", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040319067", 
          "https://doi.org/10.1038/nature03481"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03481", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040319067", 
          "https://doi.org/10.1038/nature03481"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04338", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040548022", 
          "https://doi.org/10.1038/nature04338"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04338", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040548022", 
          "https://doi.org/10.1038/nature04338"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04338", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040548022", 
          "https://doi.org/10.1038/nature04338"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.077776.108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040737496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkn425", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044990606"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1286", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052844738", 
          "https://doi.org/10.1038/nmeth.1286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07517", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052925719", 
          "https://doi.org/10.1038/nature07517"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-02", 
    "datePublishedReg": "2011-02-01", 
    "description": "Despite the ever-increasing output of Illumina sequencing data, loci with extreme base compositions are often under-represented or absent. To evaluate sources of base-composition bias, we traced genomic sequences ranging from 6% to 90% GC through the process by quantitative PCR. We identified PCR during library preparation as a principal source of bias and optimized the conditions. Our improved protocol significantly reduces amplification bias and minimizes the previously severe effects of PCR instrument and temperature ramp rate.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/gb-2011-12-2-r18", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2699326", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2529218", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1023439", 
        "issn": [
          "1474-760X", 
          "1465-6906"
        ], 
        "name": "Genome Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "12"
      }
    ], 
    "name": "Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries", 
    "pagination": "r18", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "01462dea0b177bf9b870cf6db667c17fe0f122743accd3f71ad09784ceebd02f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21338519"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100960660"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/gb-2011-12-2-r18"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1000268052"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/gb-2011-12-2-r18", 
      "https://app.dimensions.ai/details/publication/pub.1000268052"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T01:59", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000509.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2Fgb-2011-12-2-r18"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/gb-2011-12-2-r18'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/gb-2011-12-2-r18'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/gb-2011-12-2-r18'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/gb-2011-12-2-r18'


 

This table displays all metadata directly associated to this object as RDF triples.

264 TRIPLES      21 PREDICATES      64 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/gb-2011-12-2-r18 schema:about N0a1ca3efad4742568570cc1bfe8583a1
2 N0da11f8728c94a108aeaa97637a5b5b1
3 N3efa9a3d73a848ba88aa00eb5374d846
4 N4028d13263694b33a97b632013c787a2
5 N42031ce9feb14d63ae9df8cccfbbbbbd
6 N4b7cc7ffbe894284914fac57f3f0efc3
7 N5d8c5a3b46ab4306be91e12d3014c60e
8 N9e18b57029d144c599b3f05e60e67b37
9 Na62d8fa9d76542da9fdd00e5f6014675
10 Nb836c004bffd4d0aaecce534224d5fad
11 Nc2311c83d9aa43ea84c61786020f9477
12 Ne5ce0fe6e9424d919a4e2508d0e4f701
13 Ne7bd721f0a3f4d679e05ef6f4e82f2e2
14 anzsrc-for:06
15 anzsrc-for:0604
16 schema:author N6bf20cc6f8924f0f9470f30e89a8f50a
17 schema:citation sg:pub.10.1038/nature01097
18 sg:pub.10.1038/nature03481
19 sg:pub.10.1038/nature03959
20 sg:pub.10.1038/nature04338
21 sg:pub.10.1038/nature07517
22 sg:pub.10.1038/nbt.1561
23 sg:pub.10.1038/nmeth.1179
24 sg:pub.10.1038/nmeth.1270
25 sg:pub.10.1038/nmeth.1276
26 sg:pub.10.1038/nmeth.1286
27 sg:pub.10.1038/nmeth.1311
28 sg:pub.10.1038/nmeth.1507
29 sg:pub.10.1186/gb-2009-10-10-r103
30 sg:pub.10.1186/gb-2009-10-6-r60
31 https://doi.org/10.1093/bioinformatics/btp324
32 https://doi.org/10.1093/nar/gkn425
33 https://doi.org/10.1101/gr.077776.108
34 https://doi.org/10.1101/gr.091868.109
35 https://doi.org/10.1101/gr.1929904
36 https://doi.org/10.1126/science.1162986
37 https://doi.org/10.1126/science.1181498
38 https://doi.org/10.1371/journal.pone.0007778
39 schema:datePublished 2011-02
40 schema:datePublishedReg 2011-02-01
41 schema:description Despite the ever-increasing output of Illumina sequencing data, loci with extreme base compositions are often under-represented or absent. To evaluate sources of base-composition bias, we traced genomic sequences ranging from 6% to 90% GC through the process by quantitative PCR. We identified PCR during library preparation as a principal source of bias and optimized the conditions. Our improved protocol significantly reduces amplification bias and minimizes the previously severe effects of PCR instrument and temperature ramp rate.
42 schema:genre research_article
43 schema:inLanguage en
44 schema:isAccessibleForFree true
45 schema:isPartOf N4bc50889967146758e8d8b5618228705
46 N7775e2ae6ac440d0b7b6641013e7cd70
47 sg:journal.1023439
48 schema:name Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
49 schema:pagination r18
50 schema:productId N23548b09898e4b5b8856e22ac5787569
51 N2be603daea5e47de98b76c9a2a1801fa
52 N7e6adde93d154a3aa7b757d108a1a267
53 N9dc643e6482e4c01b2c87f79b86a696e
54 Nd44ef579f36741408c16be1f91ae8365
55 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000268052
56 https://doi.org/10.1186/gb-2011-12-2-r18
57 schema:sdDatePublished 2019-04-11T01:59
58 schema:sdLicense https://scigraph.springernature.com/explorer/license/
59 schema:sdPublisher N1643ba83fd154dcfba343000996faa57
60 schema:url http://link.springer.com/10.1186%2Fgb-2011-12-2-r18
61 sgo:license sg:explorer/license/
62 sgo:sdDataset articles
63 rdf:type schema:ScholarlyArticle
64 N0892fe5c2c744c22a0590803a0ad69ce rdf:first Naa688d9640b84f07a1ea4ec883c6ccae
65 rdf:rest N96282831409c467b9e82929f64db8d71
66 N0a1ca3efad4742568570cc1bfe8583a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
67 schema:name Temperature
68 rdf:type schema:DefinedTerm
69 N0da11f8728c94a108aeaa97637a5b5b1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Humans
71 rdf:type schema:DefinedTerm
72 N1643ba83fd154dcfba343000996faa57 schema:name Springer Nature - SN SciGraph project
73 rdf:type schema:Organization
74 N23548b09898e4b5b8856e22ac5787569 schema:name pubmed_id
75 schema:value 21338519
76 rdf:type schema:PropertyValue
77 N251739ab68204c3cadd23dee05f5b368 schema:name Learning Community C, Cambridge Rindge and Latin School, 459 Broadway, 02138, Cambridge, MA, USA
78 rdf:type schema:Organization
79 N2b39efbee6f94f489425baa8fa3866b8 rdf:first sg:person.01300612454.35
80 rdf:rest Nbf129b6e75964b00b90d1aa769f26a86
81 N2be603daea5e47de98b76c9a2a1801fa schema:name dimensions_id
82 schema:value pub.1000268052
83 rdf:type schema:PropertyValue
84 N3efa9a3d73a848ba88aa00eb5374d846 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Nucleic Acid Denaturation
86 rdf:type schema:DefinedTerm
87 N4028d13263694b33a97b632013c787a2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Escherichia coli
89 rdf:type schema:DefinedTerm
90 N42031ce9feb14d63ae9df8cccfbbbbbd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Genome, Human
92 rdf:type schema:DefinedTerm
93 N4b7cc7ffbe894284914fac57f3f0efc3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Genetic Loci
95 rdf:type schema:DefinedTerm
96 N4bc50889967146758e8d8b5618228705 schema:issueNumber 2
97 rdf:type schema:PublicationIssue
98 N5d8c5a3b46ab4306be91e12d3014c60e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Plasmodium falciparum
100 rdf:type schema:DefinedTerm
101 N6bf20cc6f8924f0f9470f30e89a8f50a rdf:first sg:person.01064510456.35
102 rdf:rest Ne30ee93d714047439e985e0e90446e51
103 N7775e2ae6ac440d0b7b6641013e7cd70 schema:volumeNumber 12
104 rdf:type schema:PublicationVolume
105 N7e6adde93d154a3aa7b757d108a1a267 schema:name readcube_id
106 schema:value 01462dea0b177bf9b870cf6db667c17fe0f122743accd3f71ad09784ceebd02f
107 rdf:type schema:PropertyValue
108 N811796a0115742a18162b5eab7069dd1 schema:affiliation N251739ab68204c3cadd23dee05f5b368
109 schema:familyName Chen
110 schema:givenName Wei-Sheng
111 rdf:type schema:Person
112 N859356b6dbb343daa637f1434c553ab6 schema:name Learning Community C, Cambridge Rindge and Latin School, 459 Broadway, 02138, Cambridge, MA, USA
113 rdf:type schema:Organization
114 N96282831409c467b9e82929f64db8d71 rdf:first sg:person.016012720777.47
115 rdf:rest N2b39efbee6f94f489425baa8fa3866b8
116 N9dc643e6482e4c01b2c87f79b86a696e schema:name nlm_unique_id
117 schema:value 100960660
118 rdf:type schema:PropertyValue
119 N9e18b57029d144c599b3f05e60e67b37 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Genomics
121 rdf:type schema:DefinedTerm
122 Na62d8fa9d76542da9fdd00e5f6014675 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Base Composition
124 rdf:type schema:DefinedTerm
125 Naa688d9640b84f07a1ea4ec883c6ccae schema:affiliation N859356b6dbb343daa637f1434c553ab6
126 schema:familyName Danielsson
127 schema:givenName Maxwell
128 rdf:type schema:Person
129 Nb836c004bffd4d0aaecce534224d5fad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Sequence Analysis, DNA
131 rdf:type schema:DefinedTerm
132 Nbf129b6e75964b00b90d1aa769f26a86 rdf:first sg:person.01355660531.12
133 rdf:rest Nc5ccbe1c73b4489ba993d0499be3ac0c
134 Nc17cf89f229a4d3a8527b92ae61122ac rdf:first sg:person.0645200471.91
135 rdf:rest rdf:nil
136 Nc2311c83d9aa43ea84c61786020f9477 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Real-Time Polymerase Chain Reaction
138 rdf:type schema:DefinedTerm
139 Nc5ccbe1c73b4489ba993d0499be3ac0c rdf:first sg:person.01170225154.35
140 rdf:rest Nc17cf89f229a4d3a8527b92ae61122ac
141 Nd44ef579f36741408c16be1f91ae8365 schema:name doi
142 schema:value 10.1186/gb-2011-12-2-r18
143 rdf:type schema:PropertyValue
144 Ndffc780971894520ac0d20d8f0881806 rdf:first N811796a0115742a18162b5eab7069dd1
145 rdf:rest N0892fe5c2c744c22a0590803a0ad69ce
146 Ne30ee93d714047439e985e0e90446e51 rdf:first sg:person.01235503516.60
147 rdf:rest Ndffc780971894520ac0d20d8f0881806
148 Ne5ce0fe6e9424d919a4e2508d0e4f701 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Artifacts
150 rdf:type schema:DefinedTerm
151 Ne7bd721f0a3f4d679e05ef6f4e82f2e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Genomic Library
153 rdf:type schema:DefinedTerm
154 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
155 schema:name Biological Sciences
156 rdf:type schema:DefinedTerm
157 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
158 schema:name Genetics
159 rdf:type schema:DefinedTerm
160 sg:grant.2529218 http://pending.schema.org/fundedItem sg:pub.10.1186/gb-2011-12-2-r18
161 rdf:type schema:MonetaryGrant
162 sg:grant.2699326 http://pending.schema.org/fundedItem sg:pub.10.1186/gb-2011-12-2-r18
163 rdf:type schema:MonetaryGrant
164 sg:journal.1023439 schema:issn 1465-6906
165 1474-760X
166 schema:name Genome Biology
167 rdf:type schema:Periodical
168 sg:person.01064510456.35 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
169 schema:familyName Aird
170 schema:givenName Daniel
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064510456.35
172 rdf:type schema:Person
173 sg:person.01170225154.35 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
174 schema:familyName Nusbaum
175 schema:givenName Chad
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170225154.35
177 rdf:type schema:Person
178 sg:person.01235503516.60 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
179 schema:familyName Ross
180 schema:givenName Michael G
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235503516.60
182 rdf:type schema:Person
183 sg:person.01300612454.35 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
184 schema:familyName Russ
185 schema:givenName Carsten
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300612454.35
187 rdf:type schema:Person
188 sg:person.01355660531.12 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
189 schema:familyName Jaffe
190 schema:givenName David B
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355660531.12
192 rdf:type schema:Person
193 sg:person.016012720777.47 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
194 schema:familyName Fennell
195 schema:givenName Timothy
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016012720777.47
197 rdf:type schema:Person
198 sg:person.0645200471.91 schema:affiliation https://www.grid.ac/institutes/grid.66859.34
199 schema:familyName Gnirke
200 schema:givenName Andreas
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645200471.91
202 rdf:type schema:Person
203 sg:pub.10.1038/nature01097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030736656
204 https://doi.org/10.1038/nature01097
205 rdf:type schema:CreativeWork
206 sg:pub.10.1038/nature03481 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040319067
207 https://doi.org/10.1038/nature03481
208 rdf:type schema:CreativeWork
209 sg:pub.10.1038/nature03959 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021574562
210 https://doi.org/10.1038/nature03959
211 rdf:type schema:CreativeWork
212 sg:pub.10.1038/nature04338 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040548022
213 https://doi.org/10.1038/nature04338
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/nature07517 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052925719
216 https://doi.org/10.1038/nature07517
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/nbt.1561 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008087076
219 https://doi.org/10.1038/nbt.1561
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/nmeth.1179 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001773338
222 https://doi.org/10.1038/nmeth.1179
223 rdf:type schema:CreativeWork
224 sg:pub.10.1038/nmeth.1270 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021189255
225 https://doi.org/10.1038/nmeth.1270
226 rdf:type schema:CreativeWork
227 sg:pub.10.1038/nmeth.1276 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025333243
228 https://doi.org/10.1038/nmeth.1276
229 rdf:type schema:CreativeWork
230 sg:pub.10.1038/nmeth.1286 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052844738
231 https://doi.org/10.1038/nmeth.1286
232 rdf:type schema:CreativeWork
233 sg:pub.10.1038/nmeth.1311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023653905
234 https://doi.org/10.1038/nmeth.1311
235 rdf:type schema:CreativeWork
236 sg:pub.10.1038/nmeth.1507 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005850821
237 https://doi.org/10.1038/nmeth.1507
238 rdf:type schema:CreativeWork
239 sg:pub.10.1186/gb-2009-10-10-r103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012804464
240 https://doi.org/10.1186/gb-2009-10-10-r103
241 rdf:type schema:CreativeWork
242 sg:pub.10.1186/gb-2009-10-6-r60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019838301
243 https://doi.org/10.1186/gb-2009-10-6-r60
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1093/bioinformatics/btp324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038266369
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1093/nar/gkn425 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044990606
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1101/gr.077776.108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040737496
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1101/gr.091868.109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017676111
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1101/gr.1929904 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027815825
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1126/science.1162986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021160342
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1126/science.1181498 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014947319
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1371/journal.pone.0007778 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033210750
260 rdf:type schema:CreativeWork
261 https://www.grid.ac/institutes/grid.66859.34 schema:alternateName Broad Institute
262 schema:name Genome Sequencing Platform, Broad Institute of MIT and Harvard, 320 Charles Street, 02141, Cambridge, MA, USA
263 Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 320 Charles Street, 02141, Cambridge, MA, USA
264 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...