Quake: quality-aware detection and correction of sequencing errors View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-11-29

AUTHORS

David R Kelley, Michael C Schatz, Steven L Salzberg

ABSTRACT

We introduce Quake, a program to detect and correct errors in DNA sequencing reads. Using a maximum likelihood approach incorporating quality values and nucleotide specific miscall rates, Quake achieves the highest accuracy on realistically simulated reads. We further demonstrate substantial improvements in de novo assembly and SNP detection after using Quake. Quake can be used for any size project, including more than one billion human reads, and is freely available as open source software from http://www.cbcb.umd.edu/software/quake. More... »

PAGES

r116

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/gb-2010-11-11-r116

DOI

http://dx.doi.org/10.1186/gb-2010-11-11-r116

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042567408

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21114842


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