De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-09

AUTHORS

Scott DiGuistini, Nancy Y Liao, Darren Platt, Gordon Robertson, Michael Seidel, Simon K Chan, T Roderick Docking, Inanc Birol, Robert A Holt, Martin Hirst, Elaine Mardis, Marco A Marra, Richard C Hamelin, Jörg Bohlmann, Colette Breuil, Steven JM Jones

ABSTRACT

Sequencing-by-synthesis technologies can reduce the cost of generating de novo genome assemblies. We report a method for assembling draft genome sequences of eukaryotic organisms that integrates sequence information from different sources, and demonstrate its effectiveness by assembling an approximately 32.5 Mb draft genome sequence for the forest pathogen Grosmannia clavigera, an ascomycete fungus. We also developed a method for assessing draft assemblies using Illumina paired end read data and demonstrate how we are using it to guide future sequence finishing. Our results demonstrate that eukaryotic genome sequences can be accurately assembled by combining Illumina, 454 and Sanger sequence data. More... »

PAGES

r94

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/gb-2009-10-9-r94

DOI

http://dx.doi.org/10.1186/gb-2009-10-9-r94

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013301186

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19747388


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