A haplome alignment and reference sequence of the highly polymorphic Ciona savignyi genome View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2007-03-20

AUTHORS

Kerrin S Small, Michael Brudno, Matthew M Hill, Arend Sidow

ABSTRACT

The sequence of Ciona savignyi was determined using a whole-genome shotgun strategy, but a high degree of polymorphism resulted in a fractured assembly wherein allelic sequences from the same genomic region assembled separately. We designed a multistep strategy to generate a nonredundant reference sequence from the original assembly by reconstructing and aligning the two 'haplomes' (haploid genomes). In the resultant 174 megabase reference sequence, each locus is represented once, misassemblies are corrected, and contiguity and continuity are dramatically improved. More... »

PAGES

r41-r41

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/gb-2007-8-3-r41

DOI

http://dx.doi.org/10.1186/gb-2007-8-3-r41

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018789311

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17374142


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