Computational identification of developmental enhancers: conservation and function of transcription factor binding-site clusters in Drosophila melanogaster and Drosophila pseudoobscura View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2004-08

AUTHORS

Benjamin P Berman, Barret D Pfeiffer, Todd R Laverty, Steven L Salzberg, Gerald M Rubin, Michael B Eisen, Susan E Celniker

ABSTRACT

BACKGROUND: The identification of sequences that control transcription in metazoans is a major goal of genome analysis. In a previous study, we demonstrated that searching for clusters of predicted transcription factor binding sites could discover active regulatory sequences, and identified 37 regions of the Drosophila melanogaster genome with high densities of predicted binding sites for five transcription factors involved in anterior-posterior embryonic patterning. Nine of these clusters overlapped known enhancers. Here, we report the results of in vivo functional analysis of 27 remaining clusters. RESULTS: We generated transgenic flies carrying each cluster attached to a basal promoter and reporter gene, and assayed embryos for reporter gene expression. Six clusters are enhancers of adjacent genes: giant, fushi tarazu, odd-skipped, nubbin, squeeze and pdm2; three drive expression in patterns unrelated to those of neighboring genes; the remaining 18 do not appear to have enhancer activity. We used the Drosophila pseudoobscura genome to compare patterns of evolution in and around the 15 positive and 18 false-positive predictions. Although conservation of primary sequence cannot distinguish true from false positives, conservation of binding-site clustering accurately discriminates functional binding-site clusters from those with no function. We incorporated conservation of binding-site clustering into a new genome-wide enhancer screen, and predict several hundred new regulatory sequences, including 85 adjacent to genes with embryonic patterns. CONCLUSIONS: Measuring conservation of sequence features closely linked to function--such as binding-site clusterin--makes better use of comparative sequence data than commonly used methods that examine only sequence identity. More... »

PAGES

r61

References to SciGraph publications

  • 2001-02. Initial sequencing and analysis of the human genome in NATURE
  • 1980-10. Mutations affecting segment number and polarity in Drosophila in NATURE
  • 2003-12. Conservation of regulatory elements between two species of Drosophila in BMC BIOINFORMATICS
  • 2002-12. Assessing the impact of comparative genomic sequence data on the functional annotation of the Drosophilagenome in GENOME BIOLOGY
  • 1978-12. A gene complex controlling segmentation in Drosophila in NATURE
  • 2003-07. Transcription regulation and animal diversity in NATURE
  • 1988-01. Regulation of segment polarity genes in the Drosophila blastoderm by fushi tarazu and even skipped in NATURE
  • 2002-12. Initial sequencing and comparative analysis of the mouse genome in NATURE
  • 2002-12. Systematic determination of patterns of gene expression during Drosophila embryogenesis in GENOME BIOLOGY
  • 1986-12. The correct activation of Antennapedia and bithorax complex genes requires the fushi tarazu gene in NATURE
  • 2004-12. Benchmarking tools for the alignment of functional noncoding DNA in BMC BIOINFORMATICS
  • 1988-03. Regulation of the Drosophila segmentation gene hunchback by two maternal morphogenetic centres in NATURE
  • 2002-12. Computational detection of genomic cis- regulatory modules applied to body patterning in the early Drosophila embryo in BMC BIOINFORMATICS
  • 2003-12. Statistical extraction of Drosophila cis-regulatory modules using exhaustive assessment of local word frequency in BMC BIOINFORMATICS
  • 1989-09. Krüppel requirement for knirps enhancement reflects overlapping gap gene activities in the Drosophila embryo in NATURE
  • 2001-02. Genomic strategies to identify mammalian regulatory sequences in NATURE REVIEWS GENETICS
  • 2001-03. Transcriptional regulation in Drosophila: the post-genome challenge in FUNCTIONAL & INTEGRATIVE GENOMICS
  • 2002-08. High-throughput SELEX–SAGE method for quantitative modeling of transcription-factor binding sites in NATURE BIOTECHNOLOGY
  • 2000-02. Evidence for stabilizing selection in a eukaryotic enhancer element in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/gb-2004-5-9-r61

    DOI

    http://dx.doi.org/10.1186/gb-2004-5-9-r61

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1006276884

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15345045


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    443 https://www.grid.ac/institutes/grid.469946.0 schema:alternateName J. Craig Venter Institute
    444 schema:name The Institute for Genomic Research, 9712 Medical Center Drive, 20878, Rockville, MD, USA
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    446 https://www.grid.ac/institutes/grid.47840.3f schema:alternateName University of California, Berkeley
    447 schema:name Berkeley Drosophila Genome Project, Genome Sciences Department, Life Sciences Division, Lawrence Orlando Berkeley National Laboratory, 94720, Berkeley, CA, USA
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    450 Genome Sciences Department, Genomics Division, Lawrence Orlando Berkeley National Laboratory, 94720, Berkeley, CA, USA
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