Ontology type: schema:ScholarlyArticle Open Access: True
2003-10
AUTHORSJohn Castle, Phil Garrett-Engele, Christopher D Armour, Sven J Duenwald, Patrick M Loerch, Michael R Meyer, Eric E Schadt, Roland Stoughton, Mark L Parrish, Daniel D Shoemaker, Jason M Johnson
ABSTRACTMicroarrays offer a high-resolution means for monitoring pre-mRNA splicing on a genomic scale. We have developed a novel, unbiased amplification protocol that permits labeling of entire transcripts. Also, hybridization conditions, probe characteristics, and analysis algorithms were optimized for detection of exons, exon-intron edges, and exon junctions. These optimized protocols can be used to detect small variations and isoform mixtures, map the tissue specificity of known human alternative isoforms, and provide a robust, scalable platform for high-throughput discovery of alternative splicing. More... »
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http://scigraph.springernature.com/pub.10.1186/gb-2003-4-10-r66
DOIhttp://dx.doi.org/10.1186/gb-2003-4-10-r66
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