Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2003-10

AUTHORS

John Castle, Phil Garrett-Engele, Christopher D Armour, Sven J Duenwald, Patrick M Loerch, Michael R Meyer, Eric E Schadt, Roland Stoughton, Mark L Parrish, Daniel D Shoemaker, Jason M Johnson

ABSTRACT

Microarrays offer a high-resolution means for monitoring pre-mRNA splicing on a genomic scale. We have developed a novel, unbiased amplification protocol that permits labeling of entire transcripts. Also, hybridization conditions, probe characteristics, and analysis algorithms were optimized for detection of exons, exon-intron edges, and exon junctions. These optimized protocols can be used to detect small variations and isoform mixtures, map the tissue specificity of known human alternative isoforms, and provide a robust, scalable platform for high-throughput discovery of alternative splicing. More... »

PAGES

r66

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/gb-2003-4-10-r66

DOI

http://dx.doi.org/10.1186/gb-2003-4-10-r66

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024685309

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/14519201


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