Ontology type: schema:ScholarlyArticle Open Access: True
2011-04
AUTHORSMauro De Santi, Luca Galluzzi, Simone Lucarini, Maria Filomena Paoletti, Alessandra Fraternale, Andrea Duranti, Cinzia De Marco, Mirco Fanelli, Nadia Zaffaroni, Giorgio Brandi, Mauro Magnani
ABSTRACTINTRODUCTION: Indole-3-carbinol (I3C), an autolysis product of glucosinolates present in cruciferous vegetables, and its dimeric derivative (3,3'-DIM) have been indicated as promising agents in preventing the development and progression of breast cancer. We have recently shown that I3C cyclic tetrameric derivative CTet formulated in γ-cyclodextrin (γ-CD) efficiently inhibited cellular proliferation in breast cancer cell lines. This study aims to analyze the mechanisms involved in the in vitro inhibition of cell proliferation and to evaluate the in vivo antitumor activity of CTet in a xenograft study. METHODS: Estrogen receptor-positive MCF-7 and triple-negative MDA-MB-231 breast cancer cell lines were exposed to CTet to evaluate cell cycle perturbation (propidium iodide staining and cytofluorimetric acquisition), induction of autophagic morphological features (co-localization of LC3b autophagosome marker and LAMP2a lysosome marker by immunofluorescence) and changes in protein expression (immunoblot and microarray-based gene expression analyses). To test the in vivo efficacy of CTet, female athymic nude mice inoculated with MCF-7 cells were i.p. treated with 5 mg/kg/day of CTet for five days/week for two weeks and the tumor mass was externally monitored. RESULTS: CTet induced accumulation in G2/M phase without evidence of apoptotic response induction in both cell lines tested. In triple-negative MDA-MB-231 the autophagic lysosomal activity was significantly up-regulated after exposure to 4 μM of CTet for 8 hours, while the highest CTet concentration was necessary to observe autophagic features in MCF-7 cells. The inhibition of Akt activity and p53-independent p21/CDKN1A and GADD45A overexpression were identified as the main molecular events responsible for CTet activity in MCF-7 and p53-mutant MDA-MB-231 cells. In vivo, CTet administration was able to significantly inhibit the growth of MCF-7 xenotransplanted into nude mice, without adverse effect on body weight or on haematological parameters. CONCLUSIONS: Our data support CTet formulated with γ-CD as a promising and injectable anticancer agent for both hormone-responsive and triple-negative breast tumors. More... »
PAGESr33
http://scigraph.springernature.com/pub.10.1186/bcr2855
DOIhttp://dx.doi.org/10.1186/bcr2855
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1050288369
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/21435243
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Oncology and Carcinogenesis",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Antineoplastic Agents",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Breast Neoplasms",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Cycle",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Cycle Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Line, Tumor",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Proliferation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cyclin-Dependent Kinase Inhibitor p21",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Female",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression Regulation, Neoplastic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genes, p53",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Indoles",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Lysosomal-Associated Membrane Protein 2",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Lysosome-Associated Membrane Glycoproteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice, Nude",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Nuclear Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Receptor, ErbB-2",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Receptors, Estrogen",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Receptors, Progesterone",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "gamma-Cyclodextrins",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Urbino",
"id": "https://www.grid.ac/institutes/grid.12711.34",
"name": [
"Department of Biomolecular Sciences, University of Urbino 'Carlo Bo', Via Saffi 2, 61029, Urbino, Italy"
],
"type": "Organization"
},
"familyName": "De Santi",
"givenName": "Mauro",
"id": "sg:person.0733304611.17",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733304611.17"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Urbino",
"id": "https://www.grid.ac/institutes/grid.12711.34",
"name": [
"Department of Biomolecular Sciences, University of Urbino 'Carlo Bo', Via Saffi 2, 61029, Urbino, Italy"
],
"type": "Organization"
},
"familyName": "Galluzzi",
"givenName": "Luca",
"id": "sg:person.0617701361.11",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617701361.11"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Urbino",
"id": "https://www.grid.ac/institutes/grid.12711.34",
"name": [
"Department of Health and Drug Sciences, University of Urbino 'Carlo Bo', Via Saffi 2, 61029, Urbino, Italy"
],
"type": "Organization"
},
"familyName": "Lucarini",
"givenName": "Simone",
"id": "sg:person.0770713405.61",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770713405.61"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Urbino",
"id": "https://www.grid.ac/institutes/grid.12711.34",
"name": [
"Department of Biomolecular Sciences, University of Urbino 'Carlo Bo', Via Saffi 2, 61029, Urbino, Italy"
],
"type": "Organization"
},
"familyName": "Paoletti",
"givenName": "Maria Filomena",
"id": "sg:person.01232075011.44",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232075011.44"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Urbino",
"id": "https://www.grid.ac/institutes/grid.12711.34",
"name": [
"Department of Biomolecular Sciences, University of Urbino 'Carlo Bo', Via Saffi 2, 61029, Urbino, Italy"
],
"type": "Organization"
},
"familyName": "Fraternale",
"givenName": "Alessandra",
"id": "sg:person.0700661112.98",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700661112.98"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Urbino",
"id": "https://www.grid.ac/institutes/grid.12711.34",
"name": [
"Department of Health and Drug Sciences, University of Urbino 'Carlo Bo', Via Saffi 2, 61029, Urbino, Italy"
],
"type": "Organization"
},
"familyName": "Duranti",
"givenName": "Andrea",
"id": "sg:person.0762755402.53",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762755402.53"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Istituto Nazionale dei Tumori",
"id": "https://www.grid.ac/institutes/grid.417893.0",
"name": [
"Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Via G. Venezian 1, 20133, Milano, Italy"
],
"type": "Organization"
},
"familyName": "De Marco",
"givenName": "Cinzia",
"id": "sg:person.01303374226.65",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303374226.65"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Urbino",
"id": "https://www.grid.ac/institutes/grid.12711.34",
"name": [
"Department of Biomolecular Sciences, University of Urbino 'Carlo Bo', Via Saffi 2, 61029, Urbino, Italy"
],
"type": "Organization"
},
"familyName": "Fanelli",
"givenName": "Mirco",
"id": "sg:person.01007647077.29",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007647077.29"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Istituto Nazionale dei Tumori",
"id": "https://www.grid.ac/institutes/grid.417893.0",
"name": [
"Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Via G. Venezian 1, 20133, Milano, Italy"
],
"type": "Organization"
},
"familyName": "Zaffaroni",
"givenName": "Nadia",
"id": "sg:person.0740745123.93",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0740745123.93"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Urbino",
"id": "https://www.grid.ac/institutes/grid.12711.34",
"name": [
"Department of Biomolecular Sciences, University of Urbino 'Carlo Bo', Via Saffi 2, 61029, Urbino, Italy"
],
"type": "Organization"
},
"familyName": "Brandi",
"givenName": "Giorgio",
"id": "sg:person.01052036725.31",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01052036725.31"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Urbino",
"id": "https://www.grid.ac/institutes/grid.12711.34",
"name": [
"Department of Biomolecular Sciences, University of Urbino 'Carlo Bo', Via Saffi 2, 61029, Urbino, Italy"
],
"type": "Organization"
},
"familyName": "Magnani",
"givenName": "Mauro",
"id": "sg:person.0752252542.98",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752252542.98"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1016/j.cell.2007.06.009",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001474067"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1158/0008-5472.can-07-6268",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001533456"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0140-6736(05)66544-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001690029"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0140-6736(05)66544-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001690029"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0040-4020(01)92912-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003549013"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3390/molecules15064085",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004425667"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3390/molecules15064085",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004425667"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gkn300",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005932629"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1207/s15327914nc4801_12",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008062928"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/00008469-200512000-00002",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014235051"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/00008469-200512000-00002",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014235051"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/00008469-200512000-00002",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014235051"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.bjc.6605745",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016311010",
"https://doi.org/10.1038/sj.bjc.6605745"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.canlet.2008.01.033",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017358060"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m010539200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018084977"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/366701a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020218634",
"https://doi.org/10.1038/366701a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0092-8674(04)00298-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020397235"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1056/nejmoa050518",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022130089"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1056/nejmoa050518",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022130089"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.canlet.2008.04.021",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022916876"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1039/p19870002543",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024076369"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.canlet.2010.02.014",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024098672"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.273.7.3838",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024520382"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1209031",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026918115",
"https://doi.org/10.1038/sj.onc.1209031"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1209031",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026918115",
"https://doi.org/10.1038/sj.onc.1209031"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cbi.2010.05.015",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027654786"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bj3350001",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028930368"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bj3350001",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028930368"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/bcr2574",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031566986",
"https://doi.org/10.1186/bcr2574"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m000284200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031801076"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/path.2697",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032233754"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1202650",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033669155",
"https://doi.org/10.1038/sj.onc.1202650"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.onc.1202650",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033669155",
"https://doi.org/10.1038/sj.onc.1202650"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0092-8674(93)90499-g",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034836400"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.bcp.2007.09.024",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036136621"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/bcr2634",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036776439",
"https://doi.org/10.1186/bcr2634"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/bcr2634",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036776439",
"https://doi.org/10.1186/bcr2634"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1091/mbc.6.4.387",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040584864"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1158/1078-0432.ccr-07-5023",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041626084"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3322/caac.20073",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042069193"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s1525-1578(10)60455-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042111550"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m412720200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044527684"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.phrs.2007.01.009",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046775342"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s1470-2045(07)70074-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047206353"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1753-4887.2007.tb00303.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047855829"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1753-4887.2007.tb00303.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047855829"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0140-6736(98)09201-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048838308"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/annonc/mdl498",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051931730"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/jf101806t",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055908372"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/jf101806t",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055908372"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/jo020415y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1056017043"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/jo020415y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1056017043"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/tx00026a007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1056296731"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4161/cc.4.9.1993",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1072298395"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1075203353",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/jn/133.7.2448s",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1075309944"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1075319103",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1076997388",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082984473",
"type": "CreativeWork"
}
],
"datePublished": "2011-04",
"datePublishedReg": "2011-04-01",
"description": "INTRODUCTION: Indole-3-carbinol (I3C), an autolysis product of glucosinolates present in cruciferous vegetables, and its dimeric derivative (3,3'-DIM) have been indicated as promising agents in preventing the development and progression of breast cancer. We have recently shown that I3C cyclic tetrameric derivative CTet formulated in \u03b3-cyclodextrin (\u03b3-CD) efficiently inhibited cellular proliferation in breast cancer cell lines. This study aims to analyze the mechanisms involved in the in vitro inhibition of cell proliferation and to evaluate the in vivo antitumor activity of CTet in a xenograft study.\nMETHODS: Estrogen receptor-positive MCF-7 and triple-negative MDA-MB-231 breast cancer cell lines were exposed to CTet to evaluate cell cycle perturbation (propidium iodide staining and cytofluorimetric acquisition), induction of autophagic morphological features (co-localization of LC3b autophagosome marker and LAMP2a lysosome marker by immunofluorescence) and changes in protein expression (immunoblot and microarray-based gene expression analyses). To test the in vivo efficacy of CTet, female athymic nude mice inoculated with MCF-7 cells were i.p. treated with 5 mg/kg/day of CTet for five days/week for two weeks and the tumor mass was externally monitored.\nRESULTS: CTet induced accumulation in G2/M phase without evidence of apoptotic response induction in both cell lines tested. In triple-negative MDA-MB-231 the autophagic lysosomal activity was significantly up-regulated after exposure to 4 \u03bcM of CTet for 8 hours, while the highest CTet concentration was necessary to observe autophagic features in MCF-7 cells. The inhibition of Akt activity and p53-independent p21/CDKN1A and GADD45A overexpression were identified as the main molecular events responsible for CTet activity in MCF-7 and p53-mutant MDA-MB-231 cells. In vivo, CTet administration was able to significantly inhibit the growth of MCF-7 xenotransplanted into nude mice, without adverse effect on body weight or on haematological parameters.\nCONCLUSIONS: Our data support CTet formulated with \u03b3-CD as a promising and injectable anticancer agent for both hormone-responsive and triple-negative breast tumors.",
"genre": "research_article",
"id": "sg:pub.10.1186/bcr2855",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1022375",
"issn": [
"1465-5411",
"1465-542X"
],
"name": "Breast Cancer Research",
"type": "Periodical"
},
{
"issueNumber": "2",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "13"
}
],
"name": "The indole-3-carbinol cyclic tetrameric derivative CTet inhibits cell proliferation via overexpression of p21/CDKN1A in both estrogen receptor-positive and triple-negative breast cancer cell lines",
"pagination": "r33",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"ef8f2fd602433baaf91899ddfcdfd4f89d4380abb2e74ec619d1c3e8a71302df"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"21435243"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"100927353"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1186/bcr2855"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1050288369"
]
}
],
"sameAs": [
"https://doi.org/10.1186/bcr2855",
"https://app.dimensions.ai/details/publication/pub.1050288369"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T18:21",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000516.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1186%2Fbcr2855"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/bcr2855'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/bcr2855'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/bcr2855'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/bcr2855'
This table displays all metadata directly associated to this object as RDF triples.
374 TRIPLES
21 PREDICATES
98 URIs
43 LITERALS
31 BLANK NODES