A novel approach for the generation of genetically modified mammary epithelial cell cultures yields new insights into TGFβ signaling in ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-10

AUTHORS

Ethan A Kohn, Zhijun Du, Misako Sato, Catherine MH Van Schyndle, Michael A Welsh, Yu-an Yang, Christina H Stuelten, Binwu Tang, Wenjun Ju, Erwin P Bottinger, Lalage M Wakefield

ABSTRACT

INTRODUCTION: Molecular dissection of the signaling pathways that underlie complex biological responses in the mammary epithelium is limited by the difficulty of propagating large numbers of mouse mammary epithelial cells, and by the inability of ribonucleic acid interference (RNAi)-based knockdown approaches to fully ablate gene function. Here we describe a method for the generation of conditionally immortalized mammary epithelial cells with defined genetic defects, and we show how such cells can be used to investigate complex signal transduction processes using the transforming growth factor beta (TGFβ/Smad pathway as an example. METHODS: We intercrossed the previously described H-2Kb-tsA58 transgenic mouse (Immortomouse) which expresses a temperature-sensitive mutant of the simian virus-40 large T-antigen (tsTAg), with mice of differing Smad genotypes. A panel of conditionally immortalized mammary epithelial cell (IMEC) cultures were derived from the virgin mammary glands of offspring of these crosses and used to assess the Smad dependency of different biological responses to TGFβ. RESULTS: IMECs could be propagated indefinitely at permissive temperatures and had a stable epithelial phenotype, resembling primary mammary epithelial cells with respect to several criteria, including responsiveness to TGFβ. Using this panel of cells, we demonstrated that Smad3, but not Smad2, is necessary for TGFβ-induced apoptotic, growth inhibitory and EMT responses, whereas either Smad can support TGFβ-induced invasion as long as a threshold level of total Smad is exceeded. CONCLUSIONS: This work demonstrates the practicality and utility of generating conditionally immortalized mammary epithelial cell lines from genetically modified Immortomice for detailed investigation of complex signaling pathways in the mammary epithelium. More... »

PAGES

r83

References to SciGraph publications

  • 1998-10. Clonal characterization of mouse mammary luminal epithelial and myoepithelial cells separated by fluorescence-activated cell sorting in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2006-07. Strategy for the development of a matched set of transport-competent, angiotensin receptor-deficient proximal tubule cell lines in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 1989-08. Effects of transforming growth factor β on growth of human mammary epithelial cells in culture in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY
  • 2001-01. TGF-β Signaling in Mammary Gland Development and Tumorigenesis in JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA
  • 2007-12. TGFβ–SMAD signal transduction: molecular specificity and functional flexibility in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2004-06. Induction by transforming growth factor-β1 of epithelial to mesenchymal transition is a rare event in vitro in BREAST CANCER RESEARCH
  • 2003-10. Differential regulation of TGF-β signaling through Smad2, Smad3 and Smad4 in ONCOGENE
  • 2009-06. Transforming Growth Factor-βs and Mammary Gland Involution; Functional Roles and Implications for Cancer Progression in JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA
  • 2009-06. Transforming growth factor-β in breast cancer: too much, too late in BREAST CANCER RESEARCH
  • 2009-06. Transforming growth factor-β signaling: emerging stem cell target in metastatic breast cancer? in BREAST CANCER RESEARCH AND TREATMENT
  • 2003-11. Cytostatic and apoptotic actions of TGF-β in homeostasis and cancer in NATURE REVIEWS CANCER
  • 2009-02. TGF-β-induced epithelial to mesenchymal transition in CELL RESEARCH
  • 2000-06. A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland in BREAST CANCER RESEARCH
  • 1996-02. Experimental mammary epithelial morphogenesis in anin vivo model: Evidence for distinct cellular progenitors of the ductal and lobular phenotype in BREAST CANCER RESEARCH AND TREATMENT
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/bcr2728

    DOI

    http://dx.doi.org/10.1186/bcr2728

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1009304877

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/20942910


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Apoptosis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Movement", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Epithelial Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Epithelial-Mesenchymal Transition", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Female", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Knockout Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mammary Glands, Animal", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice, Transgenic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Signal Transduction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Smad2 Protein", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Smad3 Protein", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transforming Growth Factor beta", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Center for Cancer Research", 
              "id": "https://www.grid.ac/institutes/grid.417768.b", 
              "name": [
                "Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive MSC 4255, 20892, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kohn", 
            "givenName": "Ethan A", 
            "id": "sg:person.01340717720.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340717720.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center for Cancer Research", 
              "id": "https://www.grid.ac/institutes/grid.417768.b", 
              "name": [
                "Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive MSC 4255, 20892, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Du", 
            "givenName": "Zhijun", 
            "id": "sg:person.0663427020.92", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663427020.92"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center for Cancer Research", 
              "id": "https://www.grid.ac/institutes/grid.417768.b", 
              "name": [
                "Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive MSC 4255, 20892, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sato", 
            "givenName": "Misako", 
            "id": "sg:person.01046431054.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046431054.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center for Cancer Research", 
              "id": "https://www.grid.ac/institutes/grid.417768.b", 
              "name": [
                "Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive MSC 4255, 20892, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Van Schyndle", 
            "givenName": "Catherine MH", 
            "id": "sg:person.0777655420.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777655420.29"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center for Cancer Research", 
              "id": "https://www.grid.ac/institutes/grid.417768.b", 
              "name": [
                "Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive MSC 4255, 20892, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Welsh", 
            "givenName": "Michael A", 
            "id": "sg:person.011273227631.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011273227631.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center for Cancer Research", 
              "id": "https://www.grid.ac/institutes/grid.417768.b", 
              "name": [
                "Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive MSC 4255, 20892, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "Yu-an", 
            "id": "sg:person.0615313620.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615313620.40"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center for Cancer Research", 
              "id": "https://www.grid.ac/institutes/grid.417768.b", 
              "name": [
                "Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive MSC 4255, 20892, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Stuelten", 
            "givenName": "Christina H", 
            "id": "sg:person.01320017611.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320017611.55"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center for Cancer Research", 
              "id": "https://www.grid.ac/institutes/grid.417768.b", 
              "name": [
                "Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive MSC 4255, 20892, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tang", 
            "givenName": "Binwu", 
            "id": "sg:person.01017754027.48", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01017754027.48"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Michigan\u2013Ann Arbor", 
              "id": "https://www.grid.ac/institutes/grid.214458.e", 
              "name": [
                "Department of Internal Medicine, University of Michigan, 1150 West Medical Center Drive, 48109, Ann Arbor, MI, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ju", 
            "givenName": "Wenjun", 
            "id": "sg:person.01277423312.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277423312.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Icahn School of Medicine at Mount Sinai", 
              "id": "https://www.grid.ac/institutes/grid.59734.3c", 
              "name": [
                "Division of Nephrology, Department of Medicine, Charles R Bronfman Institute for Personalized Medicine, Mount Sinai School of Medicine, 1468 Madison Avenue, 10029, New York, NY, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bottinger", 
            "givenName": "Erwin P", 
            "id": "sg:person.01005203363.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005203363.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center for Cancer Research", 
              "id": "https://www.grid.ac/institutes/grid.417768.b", 
              "name": [
                "Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive MSC 4255, 20892, Bethesda, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wakefield", 
            "givenName": "Lalage M", 
            "id": "sg:person.01011225013.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011225013.39"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/s1359-6101(99)00028-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001946883"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2009.5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004224732", 
              "https://doi.org/10.1038/cr.2009.5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10911-009-9122-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004545897", 
              "https://doi.org/10.1007/s10911-009-9122-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10911-009-9122-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004545897", 
              "https://doi.org/10.1007/s10911-009-9122-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1208", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004573220", 
              "https://doi.org/10.1038/nrc1208"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1208", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004573220", 
              "https://doi.org/10.1038/nrc1208"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1097-2765(00)80119-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009995487"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.1243205", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010903955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1009568532177", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011093311", 
              "https://doi.org/10.1023/a:1009568532177"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.01038-08", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016399772"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/1541-7786.mcr-08-0558", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016695106"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10549-008-0184-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019984071", 
              "https://doi.org/10.1007/s10549-008-0184-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10549-008-0184-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019984071", 
              "https://doi.org/10.1007/s10549-008-0184-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1091/mbc.e05-01-0054", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020253007"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1091/mbc.e05-01-0054", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020253007"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m102382200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021585459"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrm2297", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024609722", 
              "https://doi.org/10.1038/nrm2297"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.127.6.2021", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025022548"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2008.04.007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025190984"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/bcr2224", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026076149", 
              "https://doi.org/10.1186/bcr2224"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1206791", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028509858", 
              "https://doi.org/10.1038/sj.onc.1206791"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1206791", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028509858", 
              "https://doi.org/10.1038/sj.onc.1206791"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1349-7006.2009.01299.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029249582"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1349-7006.2009.01299.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029249582"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11626-998-0067-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029578453", 
              "https://doi.org/10.1007/s11626-998-0067-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jcp.20948", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031615270"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02623723", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032636003", 
              "https://doi.org/10.1007/bf02623723"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02623723", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032636003", 
              "https://doi.org/10.1007/bf02623723"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.26.2.654-667.2006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035986396"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2008.07.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037108428"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1290/0511076.1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037521640", 
              "https://doi.org/10.1290/0511076.1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01806075", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037799294", 
              "https://doi.org/10.1007/bf01806075"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01806075", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037799294", 
              "https://doi.org/10.1007/bf01806075"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/bcr778", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038103112", 
              "https://doi.org/10.1186/bcr778"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1172/jci33713", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038658278"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1354/vp.41-3-278", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039506343"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1354/vp.41-3-278", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039506343"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1834070100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039858843"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/cbir.1998.0339", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039960244"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0002-9440(10)63153-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040141304"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/bcr57", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041536221", 
              "https://doi.org/10.1186/bcr57"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.88.12.5096", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049675685"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/emboj/18.5.1280", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051719102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jcb.21255", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052052751"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/0008-5472.can-08-3961", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053235165"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1172/jci19270", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1063416394"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075035361", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075917518", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077005612", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1988.tb03048.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079733535"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082994576", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2010-10", 
        "datePublishedReg": "2010-10-01", 
        "description": "INTRODUCTION: Molecular dissection of the signaling pathways that underlie complex biological responses in the mammary epithelium is limited by the difficulty of propagating large numbers of mouse mammary epithelial cells, and by the inability of ribonucleic acid interference (RNAi)-based knockdown approaches to fully ablate gene function. Here we describe a method for the generation of conditionally immortalized mammary epithelial cells with defined genetic defects, and we show how such cells can be used to investigate complex signal transduction processes using the transforming growth factor beta (TGF\u03b2/Smad pathway as an example.\nMETHODS: We intercrossed the previously described H-2Kb-tsA58 transgenic mouse (Immortomouse) which expresses a temperature-sensitive mutant of the simian virus-40 large T-antigen (tsTAg), with mice of differing Smad genotypes. A panel of conditionally immortalized mammary epithelial cell (IMEC) cultures were derived from the virgin mammary glands of offspring of these crosses and used to assess the Smad dependency of different biological responses to TGF\u03b2.\nRESULTS: IMECs could be propagated indefinitely at permissive temperatures and had a stable epithelial phenotype, resembling primary mammary epithelial cells with respect to several criteria, including responsiveness to TGF\u03b2. Using this panel of cells, we demonstrated that Smad3, but not Smad2, is necessary for TGF\u03b2-induced apoptotic, growth inhibitory and EMT responses, whereas either Smad can support TGF\u03b2-induced invasion as long as a threshold level of total Smad is exceeded.\nCONCLUSIONS: This work demonstrates the practicality and utility of generating conditionally immortalized mammary epithelial cell lines from genetically modified Immortomice for detailed investigation of complex signaling pathways in the mammary epithelium.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1186/bcr2728", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2497349", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2710087", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2496651", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2723560", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1022375", 
            "issn": [
              "1465-5411", 
              "1465-542X"
            ], 
            "name": "Breast Cancer Research", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "5", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "12"
          }
        ], 
        "name": "A novel approach for the generation of genetically modified mammary epithelial cell cultures yields new insights into TGF\u03b2 signaling in the mammary gland", 
        "pagination": "r83", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "2c5314d66d7587371dc3d3b937414db542de7d57415eec6d85fa090005e017bf"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "20942910"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "100927353"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/bcr2728"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1009304877"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/bcr2728", 
          "https://app.dimensions.ai/details/publication/pub.1009304877"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T01:12", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000536.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1186%2Fbcr2728"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/bcr2728'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/bcr2728'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/bcr2728'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/bcr2728'


     

    This table displays all metadata directly associated to this object as RDF triples.

    353 TRIPLES      21 PREDICATES      87 URIs      37 LITERALS      25 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/bcr2728 schema:about N0872bb2ecb404c089c988573efd14d50
    2 N0c9f31c2d7634d0bb3e8cdacb73a050b
    3 N0e893159728f44239d75f68d643c836f
    4 N4507cd39eda34ba292782bd4412dcaf4
    5 N45746074a18645669a037d15fd38ec6c
    6 N553781ddc8f24b63a0a8cb5bc38f66c7
    7 N56428573c4d74de3b35a29741690588c
    8 N6ebd9cdfc98f43dda5fd0cf746aeb9af
    9 N789e17cf11b545569b0822a848ac5247
    10 N7b57b8b171ab4d4e95fdd96e95f766c0
    11 N854048f540fd43b2bf2722b678ad03d9
    12 Nb113d774e278452db0f6eb79f237e6ac
    13 Nb2b255f7a82042d9820771d077903e1a
    14 Nbd6a578a460a425bbc6c38481ba5a9f7
    15 Nc9ccda399c6c458a866adffe782f19fc
    16 Ne3ece98e64774c5e9fddd4750297842e
    17 anzsrc-for:06
    18 anzsrc-for:0601
    19 schema:author Nf04b941084354896bf4733cc56c4c21a
    20 schema:citation sg:pub.10.1007/bf01806075
    21 sg:pub.10.1007/bf02623723
    22 sg:pub.10.1007/s10549-008-0184-1
    23 sg:pub.10.1007/s10911-009-9122-z
    24 sg:pub.10.1007/s11626-998-0067-0
    25 sg:pub.10.1023/a:1009568532177
    26 sg:pub.10.1038/cr.2009.5
    27 sg:pub.10.1038/nrc1208
    28 sg:pub.10.1038/nrm2297
    29 sg:pub.10.1038/sj.onc.1206791
    30 sg:pub.10.1186/bcr2224
    31 sg:pub.10.1186/bcr57
    32 sg:pub.10.1186/bcr778
    33 sg:pub.10.1290/0511076.1
    34 https://app.dimensions.ai/details/publication/pub.1075035361
    35 https://app.dimensions.ai/details/publication/pub.1075917518
    36 https://app.dimensions.ai/details/publication/pub.1077005612
    37 https://app.dimensions.ai/details/publication/pub.1082994576
    38 https://doi.org/10.1002/j.1460-2075.1988.tb03048.x
    39 https://doi.org/10.1002/jcb.21255
    40 https://doi.org/10.1002/jcp.20948
    41 https://doi.org/10.1006/cbir.1998.0339
    42 https://doi.org/10.1016/j.cell.2008.04.007
    43 https://doi.org/10.1016/j.cell.2008.07.001
    44 https://doi.org/10.1016/s0002-9440(10)63153-7
    45 https://doi.org/10.1016/s1097-2765(00)80119-7
    46 https://doi.org/10.1016/s1359-6101(99)00028-3
    47 https://doi.org/10.1073/pnas.1834070100
    48 https://doi.org/10.1073/pnas.88.12.5096
    49 https://doi.org/10.1074/jbc.m102382200
    50 https://doi.org/10.1083/jcb.127.6.2021
    51 https://doi.org/10.1091/mbc.e05-01-0054
    52 https://doi.org/10.1093/emboj/18.5.1280
    53 https://doi.org/10.1101/gad.1243205
    54 https://doi.org/10.1111/j.1349-7006.2009.01299.x
    55 https://doi.org/10.1128/mcb.01038-08
    56 https://doi.org/10.1128/mcb.26.2.654-667.2006
    57 https://doi.org/10.1158/0008-5472.can-08-3961
    58 https://doi.org/10.1158/1541-7786.mcr-08-0558
    59 https://doi.org/10.1172/jci19270
    60 https://doi.org/10.1172/jci33713
    61 https://doi.org/10.1354/vp.41-3-278
    62 schema:datePublished 2010-10
    63 schema:datePublishedReg 2010-10-01
    64 schema:description INTRODUCTION: Molecular dissection of the signaling pathways that underlie complex biological responses in the mammary epithelium is limited by the difficulty of propagating large numbers of mouse mammary epithelial cells, and by the inability of ribonucleic acid interference (RNAi)-based knockdown approaches to fully ablate gene function. Here we describe a method for the generation of conditionally immortalized mammary epithelial cells with defined genetic defects, and we show how such cells can be used to investigate complex signal transduction processes using the transforming growth factor beta (TGFβ/Smad pathway as an example. METHODS: We intercrossed the previously described H-2Kb-tsA58 transgenic mouse (Immortomouse) which expresses a temperature-sensitive mutant of the simian virus-40 large T-antigen (tsTAg), with mice of differing Smad genotypes. A panel of conditionally immortalized mammary epithelial cell (IMEC) cultures were derived from the virgin mammary glands of offspring of these crosses and used to assess the Smad dependency of different biological responses to TGFβ. RESULTS: IMECs could be propagated indefinitely at permissive temperatures and had a stable epithelial phenotype, resembling primary mammary epithelial cells with respect to several criteria, including responsiveness to TGFβ. Using this panel of cells, we demonstrated that Smad3, but not Smad2, is necessary for TGFβ-induced apoptotic, growth inhibitory and EMT responses, whereas either Smad can support TGFβ-induced invasion as long as a threshold level of total Smad is exceeded. CONCLUSIONS: This work demonstrates the practicality and utility of generating conditionally immortalized mammary epithelial cell lines from genetically modified Immortomice for detailed investigation of complex signaling pathways in the mammary epithelium.
    65 schema:genre research_article
    66 schema:inLanguage en
    67 schema:isAccessibleForFree true
    68 schema:isPartOf Nadad312e28de440291efcb9d8866e44b
    69 Nc19f72904f2d4245870cdbbccc5ca6b0
    70 sg:journal.1022375
    71 schema:name A novel approach for the generation of genetically modified mammary epithelial cell cultures yields new insights into TGFβ signaling in the mammary gland
    72 schema:pagination r83
    73 schema:productId N1fa53d22cfb141a99b09e4d19866c34a
    74 N44e68cdc4c9a412c8fb80e5b4ce505c5
    75 Nccb599591bca4cd08b6e9fa00dcea6f8
    76 Ne92b09378eb341d988788fe93c67755e
    77 Nec34dee5148c48b4b1db8a570dc60dc3
    78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009304877
    79 https://doi.org/10.1186/bcr2728
    80 schema:sdDatePublished 2019-04-11T01:12
    81 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    82 schema:sdPublisher N4c967eda17d748f9a2f160fb6804ee5d
    83 schema:url http://link.springer.com/10.1186%2Fbcr2728
    84 sgo:license sg:explorer/license/
    85 sgo:sdDataset articles
    86 rdf:type schema:ScholarlyArticle
    87 N076853f5d286493dac86c22a6b971352 rdf:first sg:person.011273227631.45
    88 rdf:rest N77813926ea8b40a88baacca9d859f821
    89 N0872bb2ecb404c089c988573efd14d50 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Epithelial Cells
    91 rdf:type schema:DefinedTerm
    92 N0bd592a1f657495fa5751b44e71f8d5b rdf:first sg:person.0663427020.92
    93 rdf:rest Na5f0338f21b542eba6e266e442499ae5
    94 N0c9f31c2d7634d0bb3e8cdacb73a050b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name Smad2 Protein
    96 rdf:type schema:DefinedTerm
    97 N0e893159728f44239d75f68d643c836f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    98 schema:name Signal Transduction
    99 rdf:type schema:DefinedTerm
    100 N1a372573087141ab9599d0478e694644 rdf:first sg:person.0777655420.29
    101 rdf:rest N076853f5d286493dac86c22a6b971352
    102 N1b9f397456934673a140d074e0e8ca7e rdf:first sg:person.01320017611.55
    103 rdf:rest N910bf0a206df4c03943f948884763a75
    104 N1fa53d22cfb141a99b09e4d19866c34a schema:name dimensions_id
    105 schema:value pub.1009304877
    106 rdf:type schema:PropertyValue
    107 N44e68cdc4c9a412c8fb80e5b4ce505c5 schema:name pubmed_id
    108 schema:value 20942910
    109 rdf:type schema:PropertyValue
    110 N4507cd39eda34ba292782bd4412dcaf4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Female
    112 rdf:type schema:DefinedTerm
    113 N45746074a18645669a037d15fd38ec6c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Gene Knockout Techniques
    115 rdf:type schema:DefinedTerm
    116 N4c967eda17d748f9a2f160fb6804ee5d schema:name Springer Nature - SN SciGraph project
    117 rdf:type schema:Organization
    118 N553781ddc8f24b63a0a8cb5bc38f66c7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Smad3 Protein
    120 rdf:type schema:DefinedTerm
    121 N56428573c4d74de3b35a29741690588c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Mice, Transgenic
    123 rdf:type schema:DefinedTerm
    124 N6ebd9cdfc98f43dda5fd0cf746aeb9af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    125 schema:name Cell Differentiation
    126 rdf:type schema:DefinedTerm
    127 N77813926ea8b40a88baacca9d859f821 rdf:first sg:person.0615313620.40
    128 rdf:rest N1b9f397456934673a140d074e0e8ca7e
    129 N789e17cf11b545569b0822a848ac5247 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Mammary Glands, Animal
    131 rdf:type schema:DefinedTerm
    132 N7b57b8b171ab4d4e95fdd96e95f766c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Epithelial-Mesenchymal Transition
    134 rdf:type schema:DefinedTerm
    135 N854048f540fd43b2bf2722b678ad03d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    136 schema:name Cell Movement
    137 rdf:type schema:DefinedTerm
    138 N88792db4fdb54ec28998d8b5f9beb093 rdf:first sg:person.01011225013.39
    139 rdf:rest rdf:nil
    140 N910bf0a206df4c03943f948884763a75 rdf:first sg:person.01017754027.48
    141 rdf:rest Nf1b75a43ee884dee8c72a03f7f775010
    142 Na5f0338f21b542eba6e266e442499ae5 rdf:first sg:person.01046431054.37
    143 rdf:rest N1a372573087141ab9599d0478e694644
    144 Nadad312e28de440291efcb9d8866e44b schema:issueNumber 5
    145 rdf:type schema:PublicationIssue
    146 Nb113d774e278452db0f6eb79f237e6ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name Cells, Cultured
    148 rdf:type schema:DefinedTerm
    149 Nb2b255f7a82042d9820771d077903e1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Transforming Growth Factor beta
    151 rdf:type schema:DefinedTerm
    152 Nbd6a578a460a425bbc6c38481ba5a9f7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Animals
    154 rdf:type schema:DefinedTerm
    155 Nc19f72904f2d4245870cdbbccc5ca6b0 schema:volumeNumber 12
    156 rdf:type schema:PublicationVolume
    157 Nc9ccda399c6c458a866adffe782f19fc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    158 schema:name Mice
    159 rdf:type schema:DefinedTerm
    160 Nccb599591bca4cd08b6e9fa00dcea6f8 schema:name readcube_id
    161 schema:value 2c5314d66d7587371dc3d3b937414db542de7d57415eec6d85fa090005e017bf
    162 rdf:type schema:PropertyValue
    163 Ne3ece98e64774c5e9fddd4750297842e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    164 schema:name Apoptosis
    165 rdf:type schema:DefinedTerm
    166 Ne92b09378eb341d988788fe93c67755e schema:name doi
    167 schema:value 10.1186/bcr2728
    168 rdf:type schema:PropertyValue
    169 Nec34dee5148c48b4b1db8a570dc60dc3 schema:name nlm_unique_id
    170 schema:value 100927353
    171 rdf:type schema:PropertyValue
    172 Nf04b941084354896bf4733cc56c4c21a rdf:first sg:person.01340717720.45
    173 rdf:rest N0bd592a1f657495fa5751b44e71f8d5b
    174 Nf1b75a43ee884dee8c72a03f7f775010 rdf:first sg:person.01277423312.31
    175 rdf:rest Nf6efc8e0f1c44b55be80561b98acc12c
    176 Nf6efc8e0f1c44b55be80561b98acc12c rdf:first sg:person.01005203363.26
    177 rdf:rest N88792db4fdb54ec28998d8b5f9beb093
    178 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    179 schema:name Biological Sciences
    180 rdf:type schema:DefinedTerm
    181 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    182 schema:name Biochemistry and Cell Biology
    183 rdf:type schema:DefinedTerm
    184 sg:grant.2496651 http://pending.schema.org/fundedItem sg:pub.10.1186/bcr2728
    185 rdf:type schema:MonetaryGrant
    186 sg:grant.2497349 http://pending.schema.org/fundedItem sg:pub.10.1186/bcr2728
    187 rdf:type schema:MonetaryGrant
    188 sg:grant.2710087 http://pending.schema.org/fundedItem sg:pub.10.1186/bcr2728
    189 rdf:type schema:MonetaryGrant
    190 sg:grant.2723560 http://pending.schema.org/fundedItem sg:pub.10.1186/bcr2728
    191 rdf:type schema:MonetaryGrant
    192 sg:journal.1022375 schema:issn 1465-5411
    193 1465-542X
    194 schema:name Breast Cancer Research
    195 rdf:type schema:Periodical
    196 sg:person.01005203363.26 schema:affiliation https://www.grid.ac/institutes/grid.59734.3c
    197 schema:familyName Bottinger
    198 schema:givenName Erwin P
    199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005203363.26
    200 rdf:type schema:Person
    201 sg:person.01011225013.39 schema:affiliation https://www.grid.ac/institutes/grid.417768.b
    202 schema:familyName Wakefield
    203 schema:givenName Lalage M
    204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011225013.39
    205 rdf:type schema:Person
    206 sg:person.01017754027.48 schema:affiliation https://www.grid.ac/institutes/grid.417768.b
    207 schema:familyName Tang
    208 schema:givenName Binwu
    209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01017754027.48
    210 rdf:type schema:Person
    211 sg:person.01046431054.37 schema:affiliation https://www.grid.ac/institutes/grid.417768.b
    212 schema:familyName Sato
    213 schema:givenName Misako
    214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046431054.37
    215 rdf:type schema:Person
    216 sg:person.011273227631.45 schema:affiliation https://www.grid.ac/institutes/grid.417768.b
    217 schema:familyName Welsh
    218 schema:givenName Michael A
    219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011273227631.45
    220 rdf:type schema:Person
    221 sg:person.01277423312.31 schema:affiliation https://www.grid.ac/institutes/grid.214458.e
    222 schema:familyName Ju
    223 schema:givenName Wenjun
    224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277423312.31
    225 rdf:type schema:Person
    226 sg:person.01320017611.55 schema:affiliation https://www.grid.ac/institutes/grid.417768.b
    227 schema:familyName Stuelten
    228 schema:givenName Christina H
    229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320017611.55
    230 rdf:type schema:Person
    231 sg:person.01340717720.45 schema:affiliation https://www.grid.ac/institutes/grid.417768.b
    232 schema:familyName Kohn
    233 schema:givenName Ethan A
    234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340717720.45
    235 rdf:type schema:Person
    236 sg:person.0615313620.40 schema:affiliation https://www.grid.ac/institutes/grid.417768.b
    237 schema:familyName Yang
    238 schema:givenName Yu-an
    239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615313620.40
    240 rdf:type schema:Person
    241 sg:person.0663427020.92 schema:affiliation https://www.grid.ac/institutes/grid.417768.b
    242 schema:familyName Du
    243 schema:givenName Zhijun
    244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663427020.92
    245 rdf:type schema:Person
    246 sg:person.0777655420.29 schema:affiliation https://www.grid.ac/institutes/grid.417768.b
    247 schema:familyName Van Schyndle
    248 schema:givenName Catherine MH
    249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777655420.29
    250 rdf:type schema:Person
    251 sg:pub.10.1007/bf01806075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037799294
    252 https://doi.org/10.1007/bf01806075
    253 rdf:type schema:CreativeWork
    254 sg:pub.10.1007/bf02623723 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032636003
    255 https://doi.org/10.1007/bf02623723
    256 rdf:type schema:CreativeWork
    257 sg:pub.10.1007/s10549-008-0184-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019984071
    258 https://doi.org/10.1007/s10549-008-0184-1
    259 rdf:type schema:CreativeWork
    260 sg:pub.10.1007/s10911-009-9122-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1004545897
    261 https://doi.org/10.1007/s10911-009-9122-z
    262 rdf:type schema:CreativeWork
    263 sg:pub.10.1007/s11626-998-0067-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029578453
    264 https://doi.org/10.1007/s11626-998-0067-0
    265 rdf:type schema:CreativeWork
    266 sg:pub.10.1023/a:1009568532177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011093311
    267 https://doi.org/10.1023/a:1009568532177
    268 rdf:type schema:CreativeWork
    269 sg:pub.10.1038/cr.2009.5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004224732
    270 https://doi.org/10.1038/cr.2009.5
    271 rdf:type schema:CreativeWork
    272 sg:pub.10.1038/nrc1208 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004573220
    273 https://doi.org/10.1038/nrc1208
    274 rdf:type schema:CreativeWork
    275 sg:pub.10.1038/nrm2297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024609722
    276 https://doi.org/10.1038/nrm2297
    277 rdf:type schema:CreativeWork
    278 sg:pub.10.1038/sj.onc.1206791 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028509858
    279 https://doi.org/10.1038/sj.onc.1206791
    280 rdf:type schema:CreativeWork
    281 sg:pub.10.1186/bcr2224 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026076149
    282 https://doi.org/10.1186/bcr2224
    283 rdf:type schema:CreativeWork
    284 sg:pub.10.1186/bcr57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041536221
    285 https://doi.org/10.1186/bcr57
    286 rdf:type schema:CreativeWork
    287 sg:pub.10.1186/bcr778 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038103112
    288 https://doi.org/10.1186/bcr778
    289 rdf:type schema:CreativeWork
    290 sg:pub.10.1290/0511076.1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037521640
    291 https://doi.org/10.1290/0511076.1
    292 rdf:type schema:CreativeWork
    293 https://app.dimensions.ai/details/publication/pub.1075035361 schema:CreativeWork
    294 https://app.dimensions.ai/details/publication/pub.1075917518 schema:CreativeWork
    295 https://app.dimensions.ai/details/publication/pub.1077005612 schema:CreativeWork
    296 https://app.dimensions.ai/details/publication/pub.1082994576 schema:CreativeWork
    297 https://doi.org/10.1002/j.1460-2075.1988.tb03048.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079733535
    298 rdf:type schema:CreativeWork
    299 https://doi.org/10.1002/jcb.21255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052052751
    300 rdf:type schema:CreativeWork
    301 https://doi.org/10.1002/jcp.20948 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031615270
    302 rdf:type schema:CreativeWork
    303 https://doi.org/10.1006/cbir.1998.0339 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039960244
    304 rdf:type schema:CreativeWork
    305 https://doi.org/10.1016/j.cell.2008.04.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025190984
    306 rdf:type schema:CreativeWork
    307 https://doi.org/10.1016/j.cell.2008.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037108428
    308 rdf:type schema:CreativeWork
    309 https://doi.org/10.1016/s0002-9440(10)63153-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040141304
    310 rdf:type schema:CreativeWork
    311 https://doi.org/10.1016/s1097-2765(00)80119-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009995487
    312 rdf:type schema:CreativeWork
    313 https://doi.org/10.1016/s1359-6101(99)00028-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001946883
    314 rdf:type schema:CreativeWork
    315 https://doi.org/10.1073/pnas.1834070100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039858843
    316 rdf:type schema:CreativeWork
    317 https://doi.org/10.1073/pnas.88.12.5096 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049675685
    318 rdf:type schema:CreativeWork
    319 https://doi.org/10.1074/jbc.m102382200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021585459
    320 rdf:type schema:CreativeWork
    321 https://doi.org/10.1083/jcb.127.6.2021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025022548
    322 rdf:type schema:CreativeWork
    323 https://doi.org/10.1091/mbc.e05-01-0054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020253007
    324 rdf:type schema:CreativeWork
    325 https://doi.org/10.1093/emboj/18.5.1280 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051719102
    326 rdf:type schema:CreativeWork
    327 https://doi.org/10.1101/gad.1243205 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010903955
    328 rdf:type schema:CreativeWork
    329 https://doi.org/10.1111/j.1349-7006.2009.01299.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1029249582
    330 rdf:type schema:CreativeWork
    331 https://doi.org/10.1128/mcb.01038-08 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016399772
    332 rdf:type schema:CreativeWork
    333 https://doi.org/10.1128/mcb.26.2.654-667.2006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035986396
    334 rdf:type schema:CreativeWork
    335 https://doi.org/10.1158/0008-5472.can-08-3961 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053235165
    336 rdf:type schema:CreativeWork
    337 https://doi.org/10.1158/1541-7786.mcr-08-0558 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016695106
    338 rdf:type schema:CreativeWork
    339 https://doi.org/10.1172/jci19270 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063416394
    340 rdf:type schema:CreativeWork
    341 https://doi.org/10.1172/jci33713 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038658278
    342 rdf:type schema:CreativeWork
    343 https://doi.org/10.1354/vp.41-3-278 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039506343
    344 rdf:type schema:CreativeWork
    345 https://www.grid.ac/institutes/grid.214458.e schema:alternateName University of Michigan–Ann Arbor
    346 schema:name Department of Internal Medicine, University of Michigan, 1150 West Medical Center Drive, 48109, Ann Arbor, MI, USA
    347 rdf:type schema:Organization
    348 https://www.grid.ac/institutes/grid.417768.b schema:alternateName Center for Cancer Research
    349 schema:name Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, 37 Convent Drive MSC 4255, 20892, Bethesda, MD, USA
    350 rdf:type schema:Organization
    351 https://www.grid.ac/institutes/grid.59734.3c schema:alternateName Icahn School of Medicine at Mount Sinai
    352 schema:name Division of Nephrology, Department of Medicine, Charles R Bronfman Institute for Personalized Medicine, Mount Sinai School of Medicine, 1468 Madison Avenue, 10029, New York, NY, USA
    353 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...