Cloning, overexpression and biocatalytic exploration of a novel Baeyer-Villiger monooxygenase from Aspergillus fumigatus Af293 View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-12

AUTHORS

Maria Laura Mascotti, Maximiliano Juri Ayub, Hanna Dudek, Marcela Kurina Sanz, Marco W Fraaije

ABSTRACT

The presence of several putative Baeyer-Villiger Monooxygenases (BVMOs) encoding genes in Aspergillus fumigatus Af293 was demonstrated for the first time. One of the identified BVMO-encoding genes was cloned and successfully overexpressed fused to the cofactor regenerating enzyme phosphite dehydrogenase (PTDH). The enzyme named BVMOAf1 was extensively characterized in terms of its substrate scope and essential kinetic features. It showed high chemo-, regio- and stereoselectivity not only in the oxidation of asymmetric sulfides, (S)-sulfoxides were obtained with 99% ee, but also in the kinetic resolution of bicyclo[3.2.0]hept-2-en-6-one. This kinetic resolution process led to the production of (1S,5R) normal lactone and (1R,5S) abnormal lactone with a regioisomeric ratio of 1:1 and 99% ee each. Besides, different reaction conditions, such as pH, temperature and the presence of organic solvents, have been tested, revealing that BVMOAf1 is a relatively robust biocatalyst. More... »

PAGES

33

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/2191-0855-3-33

DOI

http://dx.doi.org/10.1186/2191-0855-3-33

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016784959

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23767684


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