Further characterization of autoantibodies to GABAergic neurons in the central nervous system produced by a subset of children with autism View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-04-26

AUTHORS

Sharifia Wills, Christy C Rossi, Jeffrey Bennett, Veronica Martinez-Cerdeño, Paul Ashwood, David G Amaral, Judy Van de Water

ABSTRACT

BACKGROUND: Autism is a neurodevelopmental disorder characterized by impairments in social interaction and deficits in verbal and nonverbal communication, together with the presence of repetitive behaviors or a limited repertoire of activities and interests. The causes of autism are currently unclear. In a previous study, we determined that 21% of children with autism have plasma autoantibodies that are immunoreactive with a population of neurons in the cerebellum that appear to be Golgi cells, which are GABAergic interneurons. METHODS: We have extended this analysis by examining plasma immunoreactivity in the remainder of the brain. To determine cell specificity, double-labeling studies that included one of the calcium-binding proteins that are commonly colocalized in GABAergic neurons (calbindin, parvalbumin or calretinin) were also carried out to determine which GABAergic neurons are immunoreactive. Coronal sections through the rostrocaudal extent of the macaque monkey brain were reacted with plasma from each of seven individuals with autism who had previously demonstrated positive Golgi cell staining, as well as six negative controls. In addition, brain sections from adult male mice were similarly examined. RESULTS: In each case, specific staining was observed for neurons that had the morphological appearance of interneurons. By double-labeling sections with plasma and with antibodies directed against γ-aminobutyric acid (GABA), we determined that all autoantibody-positive neurons were GABAergic. However, not all GABAergic neurons were autoantibody-positive. Calbindin was colabeled in several of the autoantibody-labeled cells, while parvalbumin colabeling was less frequently observed. Autoantibody-positive cells rarely expressed calretinin. Sections from the mouse brain processed similarly to the primate sections also demonstrated immunoreactivity to interneurons distributed throughout the neocortex and many subcortical regions. Some cell populations stained in the primate (such as the Golgi neurons in the cerebellum) were not as robustly immunoreactive in the mouse brain. CONCLUSIONS: These results suggest that the earlier report of autoantibody immunoreactivity to specific cells in the cerebellum extend to other regions of the brain. Further, these findings confirm the autoantibody-targeted cells to be a subpopulation of GABAergic interneurons. The potential impact of these autoantibodies on GABAergic disruption with respect to the etiology of autism is discussed herein. More... »

PAGES

5-5

References to SciGraph publications

  • 2008-09-23. GABAA Receptor Downregulation in Brains of Subjects with Autism in JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS
  • 1996-05-01. Immunogenetic studies in autism and related disorders in JOURNAL OF MOLECULAR NEUROSCIENCE
  • 2001-04. Letters to the Editor: Suppressed GABAergic Inhibition as a Common Factor in Suspected Etiologies of Autism in JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS
  • 1995-03-01. Autoantibodies to Neuronal Glutamate Receptors in Patients with Paraneoplastic Neurodegenerative Syndrome Enhance Receptor Activation in MOLECULAR MEDICINE
  • 2007-06-20. How Relevant are GFAP Autoantibodies in Autism and Tourette Syndrome? in JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS
  • 1997-10. Diagnosing Autism: Analyses of Data from the Autism Diagnostic Interview in JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS
  • 2001-12. Density and Distribution of Hippocampal Neurotransmitter Receptors in Autism: An Autoradiographic Study in JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS
  • 2008-05-16. Sydenham’s chorea in CURRENT TREATMENT OPTIONS IN NEUROLOGY
  • 1995-08. The Pre-Linguistic Autism Diagnostic Observation Schedule in JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS
  • 1991-04. Distribution of GABA and neuropeptides in the human cerebral cortex in BRAIN STRUCTURE AND FUNCTION
  • 2007-06-22. Are There Enhanced MBP Autoantibodies in Autism? in JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS
  • 2007-01-18. Decreased GAD67 mRNA levels in cerebellar Purkinje cells in autism: pathophysiological implications in ACTA NEUROPATHOLOGICA
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/2040-2392-2-5

    DOI

    http://dx.doi.org/10.1186/2040-2392-2-5

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1023319737

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21521495


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Neurosciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "NIEHS Center for Children's Environmental Health, University of California, Davis, Davis, CA 95616, USA", 
              "id": "http://www.grid.ac/institutes/grid.27860.3b", 
              "name": [
                "Division of Rheumatology, Allergy and Clinical Immunology, University of California at Davis, 451 Health Sciences Drive, Suite 6510 GBSF, Davis, CA 95616, USA", 
                "NIEHS Center for Children's Environmental Health, University of California, Davis, Davis, CA 95616, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wills", 
            "givenName": "Sharifia", 
            "id": "sg:person.01144647427.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144647427.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA", 
              "id": "http://www.grid.ac/institutes/grid.413079.8", 
              "name": [
                "Department of Psychiatry and Behavioral Sciences, Center for Neuroscience, California National Primate Research Center, University of California, Davis, Davis, CA 95616, USA", 
                "The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rossi", 
            "givenName": "Christy C", 
            "id": "sg:person.01031250534.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031250534.29"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA", 
              "id": "http://www.grid.ac/institutes/grid.413079.8", 
              "name": [
                "Department of Psychiatry and Behavioral Sciences, Center for Neuroscience, California National Primate Research Center, University of California, Davis, Davis, CA 95616, USA", 
                "The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bennett", 
            "givenName": "Jeffrey", 
            "id": "sg:person.012255642435.76", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012255642435.76"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA", 
              "id": "http://www.grid.ac/institutes/grid.413079.8", 
              "name": [
                "Department of Psychiatry and Behavioral Sciences, Center for Neuroscience, California National Primate Research Center, University of California, Davis, Davis, CA 95616, USA", 
                "The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Martinez-Cerde\u00f1o", 
            "givenName": "Veronica", 
            "id": "sg:person.0773501217.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0773501217.19"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "NIEHS Center for Children's Environmental Health, University of California, Davis, Davis, CA 95616, USA", 
              "id": "http://www.grid.ac/institutes/grid.27860.3b", 
              "name": [
                "Department of Medical Microbiology and Immunology, University of California at Davis, Davis, CA 95616, USA", 
                "The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA", 
                "NIEHS Center for Children's Environmental Health, University of California, Davis, Davis, CA 95616, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ashwood", 
            "givenName": "Paul", 
            "id": "sg:person.01232310117.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232310117.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA", 
              "id": "http://www.grid.ac/institutes/grid.413079.8", 
              "name": [
                "Department of Psychiatry and Behavioral Sciences, Center for Neuroscience, California National Primate Research Center, University of California, Davis, Davis, CA 95616, USA", 
                "The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Amaral", 
            "givenName": "David G", 
            "id": "sg:person.01314206025.72", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314206025.72"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "NIEHS Center for Children's Environmental Health, University of California, Davis, Davis, CA 95616, USA", 
              "id": "http://www.grid.ac/institutes/grid.27860.3b", 
              "name": [
                "Division of Rheumatology, Allergy and Clinical Immunology, University of California at Davis, 451 Health Sciences Drive, Suite 6510 GBSF, Davis, CA 95616, USA", 
                "The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA", 
                "NIEHS Center for Children's Environmental Health, University of California, Davis, Davis, CA 95616, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Van de Water", 
            "givenName": "Judy", 
            "id": "sg:person.0634643060.67", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634643060.67"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf02815207", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049113004", 
              "https://doi.org/10.1007/bf02815207"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10803-007-0400-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050612603", 
              "https://doi.org/10.1007/s10803-007-0400-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1013238809666", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014891049", 
              "https://doi.org/10.1023/a:1013238809666"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02179373", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003985272", 
              "https://doi.org/10.1007/bf02179373"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1025873925661", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012148340", 
              "https://doi.org/10.1023/a:1025873925661"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10803-007-0398-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032085358", 
              "https://doi.org/10.1007/s10803-007-0398-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf03401549", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1082846114", 
              "https://doi.org/10.1007/bf03401549"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1010715619091", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003545226", 
              "https://doi.org/10.1023/a:1010715619091"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10803-008-0646-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011875300", 
              "https://doi.org/10.1007/s10803-008-0646-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00401-006-0176-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009065431", 
              "https://doi.org/10.1007/s00401-006-0176-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00196841", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002382068", 
              "https://doi.org/10.1007/bf00196841"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11940-008-0025-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032376643", 
              "https://doi.org/10.1007/s11940-008-0025-x"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2011-04-26", 
        "datePublishedReg": "2011-04-26", 
        "description": "BACKGROUND: Autism is a neurodevelopmental disorder characterized by impairments in social interaction and deficits in verbal and nonverbal communication, together with the presence of repetitive behaviors or a limited repertoire of activities and interests. The causes of autism are currently unclear. In a previous study, we determined that 21% of children with autism have plasma autoantibodies that are immunoreactive with a population of neurons in the cerebellum that appear to be Golgi cells, which are GABAergic interneurons.\nMETHODS: We have extended this analysis by examining plasma immunoreactivity in the remainder of the brain. To determine cell specificity, double-labeling studies that included one of the calcium-binding proteins that are commonly colocalized in GABAergic neurons (calbindin, parvalbumin or calretinin) were also carried out to determine which GABAergic neurons are immunoreactive. Coronal sections through the rostrocaudal extent of the macaque monkey brain were reacted with plasma from each of seven individuals with autism who had previously demonstrated positive Golgi cell staining, as well as six negative controls. In addition, brain sections from adult male mice were similarly examined.\nRESULTS: In each case, specific staining was observed for neurons that had the morphological appearance of interneurons. By double-labeling sections with plasma and with antibodies directed against \u03b3-aminobutyric acid (GABA), we determined that all autoantibody-positive neurons were GABAergic. However, not all GABAergic neurons were autoantibody-positive. Calbindin was colabeled in several of the autoantibody-labeled cells, while parvalbumin colabeling was less frequently observed. Autoantibody-positive cells rarely expressed calretinin. Sections from the mouse brain processed similarly to the primate sections also demonstrated immunoreactivity to interneurons distributed throughout the neocortex and many subcortical regions. Some cell populations stained in the primate (such as the Golgi neurons in the cerebellum) were not as robustly immunoreactive in the mouse brain.\nCONCLUSIONS: These results suggest that the earlier report of autoantibody immunoreactivity to specific cells in the cerebellum extend to other regions of the brain. Further, these findings confirm the autoantibody-targeted cells to be a subpopulation of GABAergic interneurons. The potential impact of these autoantibodies on GABAergic disruption with respect to the etiology of autism is discussed herein.", 
        "genre": "article", 
        "id": "sg:pub.10.1186/2040-2392-2-5", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2552497", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1043709", 
            "issn": [
              "2040-2392"
            ], 
            "name": "Molecular Autism", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "2"
          }
        ], 
        "keywords": [
          "GABAergic neurons", 
          "GABAergic interneurons", 
          "mouse brain", 
          "adult male mice", 
          "Double-labeling studies", 
          "central nervous system", 
          "macaque monkey brain", 
          "subset of children", 
          "populations of neurons", 
          "GABAergic disruption", 
          "plasma autoantibodies", 
          "male mice", 
          "calcium-binding proteins", 
          "rostrocaudal extent", 
          "brain sections", 
          "nervous system", 
          "monkey brain", 
          "coronal sections", 
          "subcortical regions", 
          "plasma immunoreactivity", 
          "causes of autism", 
          "interneurons", 
          "autoantibodies", 
          "Golgi cells", 
          "etiology of autism", 
          "neurons", 
          "immunoreactivity", 
          "brain", 
          "neurodevelopmental disorders", 
          "aminobutyric acid", 
          "cell populations", 
          "specific staining", 
          "negative control", 
          "morphological appearance", 
          "cerebellum", 
          "cell specificity", 
          "cell staining", 
          "staining", 
          "repetitive behaviors", 
          "cells", 
          "earlier reports", 
          "children", 
          "limited repertoire", 
          "autism", 
          "specific cells", 
          "further characterization", 
          "previous studies", 
          "calretinin", 
          "calbindin", 
          "population", 
          "etiology", 
          "neocortex", 
          "mice", 
          "impairment", 
          "antibodies", 
          "disorders", 
          "plasma", 
          "subpopulations", 
          "colabeling", 
          "study", 
          "deficits", 
          "cause", 
          "report", 
          "primates", 
          "potential impact", 
          "findings", 
          "specificity", 
          "individuals", 
          "subset", 
          "disruption", 
          "remainder", 
          "activity", 
          "sections", 
          "control", 
          "cases", 
          "protein", 
          "nonverbal communication", 
          "appearance", 
          "repertoire", 
          "social interaction", 
          "presence", 
          "acid", 
          "extent", 
          "addition", 
          "region", 
          "impact", 
          "analysis", 
          "results", 
          "interaction", 
          "interest", 
          "respect", 
          "characterization", 
          "communication", 
          "system", 
          "behavior", 
          "positive Golgi cell staining", 
          "Golgi cell staining", 
          "double-labeling sections", 
          "autoantibody-positive neurons", 
          "autoantibody-labeled cells", 
          "parvalbumin colabeling", 
          "Autoantibody-positive cells", 
          "primate sections", 
          "autoantibody immunoreactivity", 
          "autoantibody-targeted cells"
        ], 
        "name": "Further characterization of autoantibodies to GABAergic neurons in the central nervous system produced by a subset of children with autism", 
        "pagination": "5-5", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1023319737"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/2040-2392-2-5"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "21521495"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/2040-2392-2-5", 
          "https://app.dimensions.ai/details/publication/pub.1023319737"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-01-01T18:25", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_530.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1186/2040-2392-2-5"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/2040-2392-2-5'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/2040-2392-2-5'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/2040-2392-2-5'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/2040-2392-2-5'


     

    This table displays all metadata directly associated to this object as RDF triples.

    265 TRIPLES      22 PREDICATES      143 URIs      123 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/2040-2392-2-5 schema:about anzsrc-for:11
    2 anzsrc-for:1109
    3 schema:author Naa21c84c76384c4fbce4bf1f9fbcb87b
    4 schema:citation sg:pub.10.1007/bf00196841
    5 sg:pub.10.1007/bf02179373
    6 sg:pub.10.1007/bf02815207
    7 sg:pub.10.1007/bf03401549
    8 sg:pub.10.1007/s00401-006-0176-3
    9 sg:pub.10.1007/s10803-007-0398-9
    10 sg:pub.10.1007/s10803-007-0400-6
    11 sg:pub.10.1007/s10803-008-0646-7
    12 sg:pub.10.1007/s11940-008-0025-x
    13 sg:pub.10.1023/a:1010715619091
    14 sg:pub.10.1023/a:1013238809666
    15 sg:pub.10.1023/a:1025873925661
    16 schema:datePublished 2011-04-26
    17 schema:datePublishedReg 2011-04-26
    18 schema:description BACKGROUND: Autism is a neurodevelopmental disorder characterized by impairments in social interaction and deficits in verbal and nonverbal communication, together with the presence of repetitive behaviors or a limited repertoire of activities and interests. The causes of autism are currently unclear. In a previous study, we determined that 21% of children with autism have plasma autoantibodies that are immunoreactive with a population of neurons in the cerebellum that appear to be Golgi cells, which are GABAergic interneurons. METHODS: We have extended this analysis by examining plasma immunoreactivity in the remainder of the brain. To determine cell specificity, double-labeling studies that included one of the calcium-binding proteins that are commonly colocalized in GABAergic neurons (calbindin, parvalbumin or calretinin) were also carried out to determine which GABAergic neurons are immunoreactive. Coronal sections through the rostrocaudal extent of the macaque monkey brain were reacted with plasma from each of seven individuals with autism who had previously demonstrated positive Golgi cell staining, as well as six negative controls. In addition, brain sections from adult male mice were similarly examined. RESULTS: In each case, specific staining was observed for neurons that had the morphological appearance of interneurons. By double-labeling sections with plasma and with antibodies directed against γ-aminobutyric acid (GABA), we determined that all autoantibody-positive neurons were GABAergic. However, not all GABAergic neurons were autoantibody-positive. Calbindin was colabeled in several of the autoantibody-labeled cells, while parvalbumin colabeling was less frequently observed. Autoantibody-positive cells rarely expressed calretinin. Sections from the mouse brain processed similarly to the primate sections also demonstrated immunoreactivity to interneurons distributed throughout the neocortex and many subcortical regions. Some cell populations stained in the primate (such as the Golgi neurons in the cerebellum) were not as robustly immunoreactive in the mouse brain. CONCLUSIONS: These results suggest that the earlier report of autoantibody immunoreactivity to specific cells in the cerebellum extend to other regions of the brain. Further, these findings confirm the autoantibody-targeted cells to be a subpopulation of GABAergic interneurons. The potential impact of these autoantibodies on GABAergic disruption with respect to the etiology of autism is discussed herein.
    19 schema:genre article
    20 schema:inLanguage en
    21 schema:isAccessibleForFree true
    22 schema:isPartOf N2e5e180ab8b04b3698945fe6c042887d
    23 N68599041e6b34efc9cf4d45603092c25
    24 sg:journal.1043709
    25 schema:keywords Autoantibody-positive cells
    26 Double-labeling studies
    27 GABAergic disruption
    28 GABAergic interneurons
    29 GABAergic neurons
    30 Golgi cell staining
    31 Golgi cells
    32 acid
    33 activity
    34 addition
    35 adult male mice
    36 aminobutyric acid
    37 analysis
    38 antibodies
    39 appearance
    40 autism
    41 autoantibodies
    42 autoantibody immunoreactivity
    43 autoantibody-labeled cells
    44 autoantibody-positive neurons
    45 autoantibody-targeted cells
    46 behavior
    47 brain
    48 brain sections
    49 calbindin
    50 calcium-binding proteins
    51 calretinin
    52 cases
    53 cause
    54 causes of autism
    55 cell populations
    56 cell specificity
    57 cell staining
    58 cells
    59 central nervous system
    60 cerebellum
    61 characterization
    62 children
    63 colabeling
    64 communication
    65 control
    66 coronal sections
    67 deficits
    68 disorders
    69 disruption
    70 double-labeling sections
    71 earlier reports
    72 etiology
    73 etiology of autism
    74 extent
    75 findings
    76 further characterization
    77 immunoreactivity
    78 impact
    79 impairment
    80 individuals
    81 interaction
    82 interest
    83 interneurons
    84 limited repertoire
    85 macaque monkey brain
    86 male mice
    87 mice
    88 monkey brain
    89 morphological appearance
    90 mouse brain
    91 negative control
    92 neocortex
    93 nervous system
    94 neurodevelopmental disorders
    95 neurons
    96 nonverbal communication
    97 parvalbumin colabeling
    98 plasma
    99 plasma autoantibodies
    100 plasma immunoreactivity
    101 population
    102 populations of neurons
    103 positive Golgi cell staining
    104 potential impact
    105 presence
    106 previous studies
    107 primate sections
    108 primates
    109 protein
    110 region
    111 remainder
    112 repertoire
    113 repetitive behaviors
    114 report
    115 respect
    116 results
    117 rostrocaudal extent
    118 sections
    119 social interaction
    120 specific cells
    121 specific staining
    122 specificity
    123 staining
    124 study
    125 subcortical regions
    126 subpopulations
    127 subset
    128 subset of children
    129 system
    130 schema:name Further characterization of autoantibodies to GABAergic neurons in the central nervous system produced by a subset of children with autism
    131 schema:pagination 5-5
    132 schema:productId N11e29e6965584797b2315eb8b512708d
    133 Nf90fed713be64e2e943cd9fd29ed18eb
    134 Nfbfe5b1424e2439a80ba3aad3e3a571a
    135 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023319737
    136 https://doi.org/10.1186/2040-2392-2-5
    137 schema:sdDatePublished 2022-01-01T18:25
    138 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    139 schema:sdPublisher Nf25f36c8845342b1b77173e3f95f3cc5
    140 schema:url https://doi.org/10.1186/2040-2392-2-5
    141 sgo:license sg:explorer/license/
    142 sgo:sdDataset articles
    143 rdf:type schema:ScholarlyArticle
    144 N11e29e6965584797b2315eb8b512708d schema:name dimensions_id
    145 schema:value pub.1023319737
    146 rdf:type schema:PropertyValue
    147 N2d2dec5c80754e969b9cda36e94235f8 rdf:first sg:person.0634643060.67
    148 rdf:rest rdf:nil
    149 N2e5e180ab8b04b3698945fe6c042887d schema:volumeNumber 2
    150 rdf:type schema:PublicationVolume
    151 N68599041e6b34efc9cf4d45603092c25 schema:issueNumber 1
    152 rdf:type schema:PublicationIssue
    153 Na0a964b573c74d35ac5d2c6dec4fd167 rdf:first sg:person.01314206025.72
    154 rdf:rest N2d2dec5c80754e969b9cda36e94235f8
    155 Naa21c84c76384c4fbce4bf1f9fbcb87b rdf:first sg:person.01144647427.50
    156 rdf:rest Nd1a3d8737e7d4adc951b277597077b44
    157 Naa2d44ff2a1243cb9cba63442b3d0bc8 rdf:first sg:person.01232310117.01
    158 rdf:rest Na0a964b573c74d35ac5d2c6dec4fd167
    159 Nd1a3d8737e7d4adc951b277597077b44 rdf:first sg:person.01031250534.29
    160 rdf:rest Nd4f5c5f5569346b79ad49e5d65526e4f
    161 Nd4f5c5f5569346b79ad49e5d65526e4f rdf:first sg:person.012255642435.76
    162 rdf:rest Ne7ad3d4612da405895e6d3405150c601
    163 Ne7ad3d4612da405895e6d3405150c601 rdf:first sg:person.0773501217.19
    164 rdf:rest Naa2d44ff2a1243cb9cba63442b3d0bc8
    165 Nf25f36c8845342b1b77173e3f95f3cc5 schema:name Springer Nature - SN SciGraph project
    166 rdf:type schema:Organization
    167 Nf90fed713be64e2e943cd9fd29ed18eb schema:name doi
    168 schema:value 10.1186/2040-2392-2-5
    169 rdf:type schema:PropertyValue
    170 Nfbfe5b1424e2439a80ba3aad3e3a571a schema:name pubmed_id
    171 schema:value 21521495
    172 rdf:type schema:PropertyValue
    173 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    174 schema:name Medical and Health Sciences
    175 rdf:type schema:DefinedTerm
    176 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
    177 schema:name Neurosciences
    178 rdf:type schema:DefinedTerm
    179 sg:grant.2552497 http://pending.schema.org/fundedItem sg:pub.10.1186/2040-2392-2-5
    180 rdf:type schema:MonetaryGrant
    181 sg:journal.1043709 schema:issn 2040-2392
    182 schema:name Molecular Autism
    183 schema:publisher Springer Nature
    184 rdf:type schema:Periodical
    185 sg:person.01031250534.29 schema:affiliation grid-institutes:grid.413079.8
    186 schema:familyName Rossi
    187 schema:givenName Christy C
    188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031250534.29
    189 rdf:type schema:Person
    190 sg:person.01144647427.50 schema:affiliation grid-institutes:grid.27860.3b
    191 schema:familyName Wills
    192 schema:givenName Sharifia
    193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144647427.50
    194 rdf:type schema:Person
    195 sg:person.012255642435.76 schema:affiliation grid-institutes:grid.413079.8
    196 schema:familyName Bennett
    197 schema:givenName Jeffrey
    198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012255642435.76
    199 rdf:type schema:Person
    200 sg:person.01232310117.01 schema:affiliation grid-institutes:grid.27860.3b
    201 schema:familyName Ashwood
    202 schema:givenName Paul
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232310117.01
    204 rdf:type schema:Person
    205 sg:person.01314206025.72 schema:affiliation grid-institutes:grid.413079.8
    206 schema:familyName Amaral
    207 schema:givenName David G
    208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314206025.72
    209 rdf:type schema:Person
    210 sg:person.0634643060.67 schema:affiliation grid-institutes:grid.27860.3b
    211 schema:familyName Van de Water
    212 schema:givenName Judy
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634643060.67
    214 rdf:type schema:Person
    215 sg:person.0773501217.19 schema:affiliation grid-institutes:grid.413079.8
    216 schema:familyName Martinez-Cerdeño
    217 schema:givenName Veronica
    218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0773501217.19
    219 rdf:type schema:Person
    220 sg:pub.10.1007/bf00196841 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002382068
    221 https://doi.org/10.1007/bf00196841
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1007/bf02179373 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003985272
    224 https://doi.org/10.1007/bf02179373
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1007/bf02815207 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049113004
    227 https://doi.org/10.1007/bf02815207
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1007/bf03401549 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082846114
    230 https://doi.org/10.1007/bf03401549
    231 rdf:type schema:CreativeWork
    232 sg:pub.10.1007/s00401-006-0176-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009065431
    233 https://doi.org/10.1007/s00401-006-0176-3
    234 rdf:type schema:CreativeWork
    235 sg:pub.10.1007/s10803-007-0398-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032085358
    236 https://doi.org/10.1007/s10803-007-0398-9
    237 rdf:type schema:CreativeWork
    238 sg:pub.10.1007/s10803-007-0400-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050612603
    239 https://doi.org/10.1007/s10803-007-0400-6
    240 rdf:type schema:CreativeWork
    241 sg:pub.10.1007/s10803-008-0646-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011875300
    242 https://doi.org/10.1007/s10803-008-0646-7
    243 rdf:type schema:CreativeWork
    244 sg:pub.10.1007/s11940-008-0025-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1032376643
    245 https://doi.org/10.1007/s11940-008-0025-x
    246 rdf:type schema:CreativeWork
    247 sg:pub.10.1023/a:1010715619091 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003545226
    248 https://doi.org/10.1023/a:1010715619091
    249 rdf:type schema:CreativeWork
    250 sg:pub.10.1023/a:1013238809666 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014891049
    251 https://doi.org/10.1023/a:1013238809666
    252 rdf:type schema:CreativeWork
    253 sg:pub.10.1023/a:1025873925661 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012148340
    254 https://doi.org/10.1023/a:1025873925661
    255 rdf:type schema:CreativeWork
    256 grid-institutes:grid.27860.3b schema:alternateName NIEHS Center for Children's Environmental Health, University of California, Davis, Davis, CA 95616, USA
    257 schema:name Department of Medical Microbiology and Immunology, University of California at Davis, Davis, CA 95616, USA
    258 Division of Rheumatology, Allergy and Clinical Immunology, University of California at Davis, 451 Health Sciences Drive, Suite 6510 GBSF, Davis, CA 95616, USA
    259 NIEHS Center for Children's Environmental Health, University of California, Davis, Davis, CA 95616, USA
    260 The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA
    261 rdf:type schema:Organization
    262 grid-institutes:grid.413079.8 schema:alternateName The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA
    263 schema:name Department of Psychiatry and Behavioral Sciences, Center for Neuroscience, California National Primate Research Center, University of California, Davis, Davis, CA 95616, USA
    264 The M.I.N.D. Institute, University of California Davis Medical Center, Sacramento, CA 95817 USA
    265 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...