An RNA isolation system for plant tissues rich in secondary metabolites View Full Text


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Article Info

DATE

2011-12

AUTHORS

Sanjay Ghawana, Asosii Paul, Hitesh Kumar, Arun Kumar, Harsharan Singh, Pardeep K Bhardwaj, Arti Rani, Ravi S Singh, Jyoti Raizada, Kashmir Singh, Sanjay Kumar

ABSTRACT

BACKGROUND: Secondary metabolites are reported to interfere with the isolation of RNA particularly with the recipes that use guanidinium-based salt. Such interference was observed in isolation of RNA with medicinal plants rheum (Rheum australe) and arnebia (Arnebia euchroma). A rapid and less cumbersome system for isolation of RNA was essential to facilitate any study related to gene expression. FINDINGS: An RNA isolation system free of guanidinium salt was developed that successfully isolated RNA from rheum and arnebia. The method took about 45 min and was successfully evaluated on twenty one tissues with varied secondary metabolites. The A260/280 ratio ranged between 1.8 - 2.0 with distinct 28 S and 18 S rRNA bands visible on a formaldehyde-agarose gel. CONCLUSIONS: The present manuscript describes a rapid protocol for isolation of RNA, which works well with all the tissues examined so far. The remarkable feature was the success in isolation of RNA with those tissues, wherein the most commonly used methods failed. Isolated RNA was amenable to downstream applications such as reverse transcription-polymerase chain reaction (RT-PCR), differential display (DD), suppression subtractive hybridization (SSH) library construction, and northern hybridization. More... »

PAGES

85

References to SciGraph publications

  • 2010-08. Light and temperature regulated terpene biosynthesis: hepatoprotective monoterpene picroside accumulation in Picrorhiza kurrooa in FUNCTIONAL & INTEGRATIVE GENOMICS
  • 2010-12. Expression of 3-hydroxy-3-methylglutaryl-CoA reductase, p-hydroxybenzoate-m-geranyltransferase and genes of phenylpropanoid pathway exhibits positive correlation with shikonins content in arnebia [Arnebia euchroma (Royle) Johnston] in BMC MOLECULAR BIOLOGY
  • 1998-03. ISPMB News 6th International Congress of Plant Molecular Biology, Quebec City, June 2000 in PLANT MOLECULAR BIOLOGY REPORTER
  • 2000-03. A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites in PLANT MOLECULAR BIOLOGY REPORTER
  • 1993-06. A simple and efficient method for isolating RNA from pine trees in PLANT MOLECULAR BIOLOGY REPORTER
  • 2010-02. Characterization of gene expression of QM from Caragana jubata, a plant species that grows under extreme cold in MOLECULAR BIOLOGY REPORTS
  • 2009-02. Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea in FUNCTIONAL & INTEGRATIVE GENOMICS
  • 2001-06. RNA isolation from high-phenolic tea leaves and apical buds in PLANT MOLECULAR BIOLOGY REPORTER
  • 1999-03. An Improved RNA Isolation Method for Plant Tissues Containing High Levels of Phenolic Compounds or Carbohydrates in PLANT MOLECULAR BIOLOGY REPORTER
  • 2008-01. Development of an Efficient Protocol of RNA Isolation from Recalcitrant Tree Tissues in MOLECULAR BIOTECHNOLOGY
  • 2009-05. p-Coumarate:CoA ligase as a key gene in the yield of catechins in tea [Camellia sinensis (L.) O. Kuntze] in FUNCTIONAL & INTEGRATIVE GENOMICS
  • 2010-02. Early low-temperature responsive mitogen activated protein kinases RaMPK1 and RaMPK2 from Rheum australe D. Don respond differentially to diverse stresses in MOLECULAR BIOLOGY REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/1756-0500-4-85

    DOI

    http://dx.doi.org/10.1186/1756-0500-4-85

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1031794416

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21443767


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