Increased expression of Ero1L-alpha in healing fetal wounds View Full Text


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Article Info

DATE

2011-06-06

AUTHORS

Phillip H Gallo, Latha Satish, Sandra Johnson, Sandeep Kathju

ABSTRACT

BackgroundAdult mammalian tissues heal injury to the skin with formation of scar; this process quickly seals an injured area, however, excessive scar formation can become a source of persistent pathology, interfering with multiple vital functions. In contrast, mammalian fetal tissue can heal without scar formation. We previously sought to model scarless healing in a rabbit fetal skin wound and identified gene products differentially expressed during fetal wound healing through PCR suppression subtractive hybridization (PCR SSH). One of these transcripts, previously identified simply as clone 11, showed putative increased expression in wounded fetal skin. This study establishes its identity as Ero1L-alpha and confirms its elevated expression in healing fetal wounds.FindingsAfter obtaining further sequence by 5' rapid amplification of cloned ends (RACE) we find that clone 11 is Ero1L-alpha. We determined that clone 11, a differentially expressed transcript in fetal wound healing, comprises the 3' untranslated region (UTR) of an approximately 4 kb transcript in rabbit tissues that corresponds to Ero1L-alpha. We showed that Ero1L-alpha is expressed predominantly as two transcripts in rabbit skin, namely a 1.6 kb transcript and the 4.0 kb transcript recovered in our PCR SSH screen via its 3' UTR sequence. However, a third transcript of 2.9 kb was also detected in Northern blots and was subsequently cloned and confirmed by 3' RACE. Knockdown of the clone 11 sequence in rabbit adult fibroblasts via siRNA resulted in significantly decreased Ero1L-alpha message expression. Increased expression of clone 11 (Ero1L-alpha) in a variety of cell types during the wound healing response was also confirmed by in situ hybridization.ConclusionsEro1L-alpha is one of the previously unknown clones identified in a PCR SSH screen for genes differentially expressed in fetal wounded tissue. In situ hybridization confirms that Ero1L-alpha shows increased expression in multiple cell types after wounding of the fetal integument. More... »

PAGES

175

References to SciGraph publications

  • 2003-01. Fetal Wound Healing: Current Biology in WORLD JOURNAL OF SURGERY
  • 2006. NCBI in ENCYCLOPEDIC REFERENCE OF GENOMICS AND PROTEOMICS IN MOLECULAR MEDICINE
  • 1979-01. Intrauterine wound healing in a 20 week human fetus in VIRCHOWS ARCHIV A PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/1756-0500-4-175

    DOI

    http://dx.doi.org/10.1186/1756-0500-4-175

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014872872

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21645405


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