In vivo activity of plant-based interleukin-12 in the lung of Balb/c mouse View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-05-27

AUTHORS

Carla Sánchez-Hernández, Abel Gutiérrez-Ortega, Diana Aguilar-León, Rogelio Hernández-Pando, Miguel Gómez-Lim, Beatriz Gómez-García

ABSTRACT

BACKGROUND: In the last years, plants are being used for the production of a wide variety of biopharmaceuticals, including cytokines, and have the potential to serve as vehicles for mucosal administration of these molecules. We had previously reported the expression of a cytokine, interleukin-12 (IL-12), in transgenic tomato plants and had demonstrated that it retained its biologic activity in vitro. FINDINGS: In this work, we administered crude extracts of IL-12-containing tomato fruits to mice through the intratracheal route, measuring endogenous IL-12 and determining biologic activity by quantification of interferon-gamma (IFN-gamma) in lungs and by histological analysis. IFN-gamma expression in lungs, as well as histological analysis, indicate that tomato-expressed IL-12 retains its biologic activity and, most importantly, its effects are restricted to the site of administration. CONCLUSION: Our results indicate that the functional activity of tomato-expressed IL-12 is comparable to that of commercial recombinant IL-12 when given via the mucosal route. This opens the possibility of using crude extracts prepared from tomatoes expressing IL-12 for certain immunotherapies. More... »

PAGES

151-151

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1756-0500-3-151

DOI

http://dx.doi.org/10.1186/1756-0500-3-151

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031483805

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20507618


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