Kinetic study of batch and fed-batch enzymatic saccharification of pretreated substrate and subsequent fermentation to ethanol View Full Text


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Article Info

DATE

2012-03-20

AUTHORS

Rishi Gupta, Sanjay Kumar, James Gomes, Ramesh Chander Kuhad

ABSTRACT

BackgroundEnzymatic hydrolysis, the rate limiting step in the process development for biofuel, is always hampered by its low sugar concentration. High solid enzymatic saccharification could solve this problem but has several other drawbacks such as low rate of reaction. In the present study we have attempted to enhance the concentration of sugars in enzymatic hydrolysate of delignified Prosopis juliflora, using a fed-batch enzymatic hydrolysis approach.ResultsThe enzymatic hydrolysis was carried out at elevated solid loading up to 20% (w/v) and a comparison kinetics of batch and fed-batch enzymatic hydrolysis was carried out using kinetic regimes. Under batch mode, the actual sugar concentration values at 20% initial substrate consistency were found deviated from the predicted values and the maximum sugar concentration obtained was 80.78 g/L. Fed-batch strategy was implemented to enhance the final sugar concentration to 127 g/L. The batch and fed-batch enzymatic hydrolysates were fermented with Saccharomyces cerevisiae and ethanol production of 34.78 g/L and 52.83 g/L, respectively, were achieved. Furthermore, model simulations showed that higher insoluble solids in the feed resulted in both smaller reactor volume and shorter residence time.ConclusionFed-batch enzymatic hydrolysis is an efficient procedure for enhancing the sugar concentration in the hydrolysate. Restricting the process to suitable kinetic regimes could result in higher conversion rates. More... »

PAGES

16

References to SciGraph publications

  • 2009-06-08. Yield-determining factors in high-solids enzymatic hydrolysis of lignocellulose in BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
  • 2002-07-17. A review of the production of ethanol from softwood in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2008-02. How biotech can transform biofuels in NATURE BIOTECHNOLOGY
  • 2008-05-30. Model-Based Fed-Batch for High-Solids Enzymatic Cellulose Hydrolysis in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2003-03. Dilute-sulfuric acid pretreatment of corn stover in pilot-scale reactor in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1186/1754-6834-5-16

    DOI

    http://dx.doi.org/10.1186/1754-6834-5-16

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1043873897

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22433563


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