Ontology type: schema:ScholarlyArticle Open Access: True
2013-12
AUTHORSCyrina D van Beusekom, Jeroen JMW van den Heuvel, Jan B Koenderink, Johannes A Schrickx, Frans GM Russel
ABSTRACTBACKGROUND: The bile salt export pump (BSEP/ABCB11) is the primary transporter for the excretion of bile acids from hepatocytes into bile. In human, inhibition of BSEP by drugs has been related to drug-induced cholestasis and subsequent cytotoxic effects. The role of BSEP in canine and feline liver diseases has not been studied in detail, but the same mechanism of inhibition by drugs as in humans could play a role in veterinary medicine. The aim of this study was to investigate the functional characteristics of feline Bsep in comparison with canine and human Bsep/BSEP with respect to substrate affinities and inhibitory potential of model drugs. Orthologs of all three species were cloned and cell membrane vesicles overexpressing feline, canine and human Bsep/BSEP were prepared for functional analyses. RESULTS: The cDNA sequences of the open reading frames of feline, canine and human Bsep/BSEP showed a high similarity between the species. Functional studies demonstrated for all species a tendency to a higher affinity of BSEP/Bsep for the conjugated bile acid taurocholic acid (TCA) than glycocholic acid (GCA), and a higher affinity for GCA than for the unconjugated cholic acid (CA). The inhibitory potency of the model inhibitors cyclosporine A, troglitazone and ketoconazole was characterized against TCA uptake into BSEP/Bsep containing membrane vesicles. All three substances potently inhibited TCA uptake without significant species differences. CONCLUSION: Structure and functional characteristics of cat, dog and human Bsep/BSEP appeared to be very similar, indicating that the properties of this transporter have been highly preserved among the different species. Therefore, inhibition of BSEP by drugs could also be a mechanism in cholestasis and liver disease in veterinary relevant animal species. This model could be used to predict drug-induced liver injury caused by BSEP inhibition at an early stage in veterinary drug development. More... »
PAGES259
http://scigraph.springernature.com/pub.10.1186/1746-6148-9-259
DOIhttp://dx.doi.org/10.1186/1746-6148-9-259
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1033525168
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/24359682
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1115",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Pharmacology and Pharmaceutical Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "ATP Binding Cassette Subfamily B Member 11",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "ATP-Binding Cassette Transporters",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Amino Acid Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Base Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Bile Acids and Salts",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cats",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cloning, Molecular",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Dogs",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "HEK293 Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Liver",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Liver Diseases",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Molecular Sequence Data",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Utrecht University",
"id": "https://www.grid.ac/institutes/grid.5477.1",
"name": [
"Veterinary Pharmacology, Pharmacotherapy and Toxicology, Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3584 CM, Utrecht, The Netherlands"
],
"type": "Organization"
},
"familyName": "van Beusekom",
"givenName": "Cyrina D",
"id": "sg:person.01011423446.75",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011423446.75"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Radboud University Nijmegen Medical Centre",
"id": "https://www.grid.ac/institutes/grid.10417.33",
"name": [
"Department of Pharmacology and Toxicology, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, Geert Grooteplein 28, 6500 HB, Nijmegen, The Netherlands"
],
"type": "Organization"
},
"familyName": "van den Heuvel",
"givenName": "Jeroen JMW",
"id": "sg:person.012163250164.42",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012163250164.42"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Radboud University Nijmegen Medical Centre",
"id": "https://www.grid.ac/institutes/grid.10417.33",
"name": [
"Department of Pharmacology and Toxicology, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, Geert Grooteplein 28, 6500 HB, Nijmegen, The Netherlands"
],
"type": "Organization"
},
"familyName": "Koenderink",
"givenName": "Jan B",
"id": "sg:person.0622147443.35",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622147443.35"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Utrecht University",
"id": "https://www.grid.ac/institutes/grid.5477.1",
"name": [
"Veterinary Pharmacology, Pharmacotherapy and Toxicology, Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3584 CM, Utrecht, The Netherlands"
],
"type": "Organization"
},
"familyName": "Schrickx",
"givenName": "Johannes A",
"id": "sg:person.01173765246.12",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173765246.12"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Radboud University Nijmegen Medical Centre",
"id": "https://www.grid.ac/institutes/grid.10417.33",
"name": [
"Department of Pharmacology and Toxicology, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, Geert Grooteplein 28, 6500 HB, Nijmegen, The Netherlands"
],
"type": "Organization"
},
"familyName": "Russel",
"givenName": "Frans GM",
"id": "sg:person.014242160457.13",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014242160457.13"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/978-3-642-14541-4_5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000210975",
"https://doi.org/10.1007/978-3-642-14541-4_5"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-3-642-14541-4_5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000210975",
"https://doi.org/10.1007/978-3-642-14541-4_5"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s1089-3261(05)70114-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000325099"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s1089-3261(05)70114-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000325099"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1124/dmd.110.037812",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003707330"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2133/dmpk.18.16",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007045215"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.273.16.10046",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007094330"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s1090-0233(03)00118-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009738759"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s1090-0233(03)00118-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009738759"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1292/jvms.53.81",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018283365"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1365-2885.2005.00647.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019603334"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-2697(76)90527-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025529346"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0195-5616(93)50311-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028858019"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0021-9673(01)87090-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028943736"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.clinre.2012.06.006",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029415668"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/path.3019",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029670240"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/bdd.629",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034844444"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1053/gast.2002.36587",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037431578"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1053/gast.2002.36591",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039768328"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1742-4658.2009.07072.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046052833"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1742-4658.2009.07072.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046052833"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/emboj/16.9.2251",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049946449"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2174/18723128112066660018",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069225818"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074751534",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1124/mol.59.3.627",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1074754429"
],
"type": "CreativeWork"
}
],
"datePublished": "2013-12",
"datePublishedReg": "2013-12-01",
"description": "BACKGROUND: The bile salt export pump (BSEP/ABCB11) is the primary transporter for the excretion of bile acids from hepatocytes into bile. In human, inhibition of BSEP by drugs has been related to drug-induced cholestasis and subsequent cytotoxic effects. The role of BSEP in canine and feline liver diseases has not been studied in detail, but the same mechanism of inhibition by drugs as in humans could play a role in veterinary medicine. The aim of this study was to investigate the functional characteristics of feline Bsep in comparison with canine and human Bsep/BSEP with respect to substrate affinities and inhibitory potential of model drugs. Orthologs of all three species were cloned and cell membrane vesicles overexpressing feline, canine and human Bsep/BSEP were prepared for functional analyses.\nRESULTS: The cDNA sequences of the open reading frames of feline, canine and human Bsep/BSEP showed a high similarity between the species. Functional studies demonstrated for all species a tendency to a higher affinity of BSEP/Bsep for the conjugated bile acid taurocholic acid (TCA) than glycocholic acid (GCA), and a higher affinity for GCA than for the unconjugated cholic acid (CA). The inhibitory potency of the model inhibitors cyclosporine A, troglitazone and ketoconazole was characterized against TCA uptake into BSEP/Bsep containing membrane vesicles. All three substances potently inhibited TCA uptake without significant species differences.\nCONCLUSION: Structure and functional characteristics of cat, dog and human Bsep/BSEP appeared to be very similar, indicating that the properties of this transporter have been highly preserved among the different species. Therefore, inhibition of BSEP by drugs could also be a mechanism in cholestasis and liver disease in veterinary relevant animal species. This model could be used to predict drug-induced liver injury caused by BSEP inhibition at an early stage in veterinary drug development.",
"genre": "research_article",
"id": "sg:pub.10.1186/1746-6148-9-259",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1035490",
"issn": [
"1746-6148"
],
"name": "BMC Veterinary Research",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "9"
}
],
"name": "The feline bile salt export pump: a structural and functional comparison with canine and human Bsep/BSEP",
"pagination": "259",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"9fb4988ca4d0f1fca89192cc3933ba5afd3d05508c5a56d6f27cea60998c43f3"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"24359682"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"101249759"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1186/1746-6148-9-259"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1033525168"
]
}
],
"sameAs": [
"https://doi.org/10.1186/1746-6148-9-259",
"https://app.dimensions.ai/details/publication/pub.1033525168"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T01:07",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000514.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1186%2F1746-6148-9-259"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1746-6148-9-259'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1746-6148-9-259'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1746-6148-9-259'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1746-6148-9-259'
This table displays all metadata directly associated to this object as RDF triples.
218 TRIPLES
21 PREDICATES
64 URIs
35 LITERALS
23 BLANK NODES