An efficient flat-surface collar-free grafting method for Arabidopsis thaliana seedlings View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-05-04

AUTHORS

Nayelli Marsch-Martínez, John Franken, Karla L Gonzalez-Aguilera, Stefan de Folter, Gerco Angenent, Elena R Alvarez-Buylla

ABSTRACT

BackgroundGrafting procedures are an excellent tool to study long range signalling processes within a plant. In the last decade, suitable flat-surface grafting procedures for young Arabidopsis seedlings using a collar to support the graft have been developed, allowing the study of long-range signals from a molecular perspective.ResultsIn the modification presented here, scion and stock are put together on the medium without supporting elements, while cotyledons are removed from the scion, resulting in increased grafting success that can reach up to 100%. At the same time, the protocol enables to process as many as 36 seedlings per hour, which combined with the high success percentage represents increased efficiency per time unit.ConclusionsGrowing cotyledons usually push the scion and the rootstock away in the absence of a supporting element. Removing them at the grafting step greatly improved success rate and reduced post-grafting manipulations. More... »

PAGES

14

References to SciGraph publications

  • <error retrieving object. in <ERROR RETRIEVING OBJECT
  • 2010-04-13. Phloem small RNAs, nutrient stress responses, and systemic mobility in BMC PLANT BIOLOGY
  • 2006-03-15. Grafting in ARABIDOPSIS PROTOCOLS
  • 2012-12-19. Inflorescence stem grafting made easy in Arabidopsis in PLANT METHODS
  • 2010-06-28. Grafting as a Research Tool in PLANT DEVELOPMENTAL BIOLOGY
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1186/1746-4811-9-14

    DOI

    http://dx.doi.org/10.1186/1746-4811-9-14

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1016282030

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23641687


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