Ontology type: schema:ScholarlyArticle Open Access: True
2012-12
AUTHORSJulie M Belanger, Yossef Raviv, Mathias Viard, Ulrich Baxa, Robert Blumenthal
ABSTRACTBACKGROUND: It has been previously shown that enveloped viruses can be inactivated using aryl azides, such as 1-iodo-5-azidonaphthalene (INA), plus UVA irradiation with preservation of surface epitopes in the inactivated virus preparations. Prolonged UVA irradiation in the presence of INA results in ROS-species formation, which in turn results in detergent resistant viral protein fractions. RESULTS: Herein, we characterize the applicability of this technique to inactivate influenza. It is shown that influenza virus + INA (100 micromolar) + UVA irradiation for 30 minutes results in a significant (p < 0.05) increase in pelletablehemagglutinin after Triton X-100 treatment followed by ultracentrifugation. Additionally, characterization of the virus suspension by immunogold labeling in cryo-EM, and viral pellet characterization via immunoprecipitation with a neutralizing antibody, shows preservation of neutralization epitopes after this treatment. CONCLUSION: These orthogonally inactivated viral preparations with detergent resistant fractions are being explored as a novel route for safe, effective inactivated vaccines generated from a variety of enveloped viruses. More... »
PAGES72
http://scigraph.springernature.com/pub.10.1186/1743-422x-9-72
DOIhttp://dx.doi.org/10.1186/1743-422x-9-72
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1023021965
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/22449007
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical Microbiology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Antiviral Agents",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Detergents",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Influenza Vaccines",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Orthomyxoviridae",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Ultraviolet Rays",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Virus Inactivation",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "King's College",
"id": "https://www.grid.ac/institutes/grid.419785.6",
"name": [
"Center for Cancer Research Nanobiology Program, National Cancer Institute Frederick, Frederick, USA",
"Department of Chemistry and Physics, King's College, Wilkes-Barre, PA, USA"
],
"type": "Organization"
},
"familyName": "Belanger",
"givenName": "Julie M",
"id": "sg:person.01142331501.69",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142331501.69"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Leidos (United States)",
"id": "https://www.grid.ac/institutes/grid.419407.f",
"name": [
"Basic Research Program, SAIC-Frederick, Inc., NCI-Frederick, 21702, Frederick, Maryland, USA"
],
"type": "Organization"
},
"familyName": "Raviv",
"givenName": "Yossef",
"id": "sg:person.01251671127.06",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251671127.06"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Leidos (United States)",
"id": "https://www.grid.ac/institutes/grid.419407.f",
"name": [
"Basic Research Program, SAIC-Frederick, Inc., NCI-Frederick, 21702, Frederick, Maryland, USA"
],
"type": "Organization"
},
"familyName": "Viard",
"givenName": "Mathias",
"id": "sg:person.01047331332.06",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047331332.06"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Leidos (United States)",
"id": "https://www.grid.ac/institutes/grid.419407.f",
"name": [
"Advanced Technology Program, SAIC-Frederick, Inc., NCI-Frederick, 21702, Frederick, Maryland, USA"
],
"type": "Organization"
},
"familyName": "Baxa",
"givenName": "Ulrich",
"id": "sg:person.01116315441.38",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01116315441.38"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Frederick National Laboratory for Cancer Research",
"id": "https://www.grid.ac/institutes/grid.418021.e",
"name": [
"Center for Cancer Research Nanobiology Program, National Cancer Institute Frederick, Frederick, USA"
],
"type": "Organization"
},
"familyName": "Blumenthal",
"givenName": "Robert",
"id": "sg:person.0622515432.21",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622515432.21"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1016/j.virol.2011.06.007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000876185"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1743-422x-3-63",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000911842",
"https://doi.org/10.1186/1743-422x-3-63"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0264-410x(90)90133-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001306219"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0264-410x(90)90133-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001306219"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jss.400010308",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001378662"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jss.400010308",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001378662"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jviromet.2005.01.016",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003264997"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jcm.02045-09",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003930238"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1600-0854.2004.00237.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006755141"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1751-1097.2010.00780.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012053892"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1751-1097.2010.00780.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012053892"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jvi.79.19.12394-12400.2005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014547247"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/0471729256.mc15g01s3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021702937"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0005-2736(74)90254-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024671459"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0005-2736(74)90254-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024671459"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1750-2659.2008.00038.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026345459"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1201/9780203911594.ch11",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030399594"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrmicro1819",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031201834",
"https://doi.org/10.1038/nrmicro1819"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jvi.02233-07",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034612310"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.bbrc.2007.04.115",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048069924"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1086/520605",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053714396"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00601a020",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055182261"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/0022-1317-66-6-1327",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060355769"
],
"type": "CreativeWork"
}
],
"datePublished": "2012-12",
"datePublishedReg": "2012-12-01",
"description": "BACKGROUND: It has been previously shown that enveloped viruses can be inactivated using aryl azides, such as 1-iodo-5-azidonaphthalene (INA), plus UVA irradiation with preservation of surface epitopes in the inactivated virus preparations. Prolonged UVA irradiation in the presence of INA results in ROS-species formation, which in turn results in detergent resistant viral protein fractions.\nRESULTS: Herein, we characterize the applicability of this technique to inactivate influenza. It is shown that influenza virus + INA (100 micromolar) + UVA irradiation for 30 minutes results in a significant (p < 0.05) increase in pelletablehemagglutinin after Triton X-100 treatment followed by ultracentrifugation. Additionally, characterization of the virus suspension by immunogold labeling in cryo-EM, and viral pellet characterization via immunoprecipitation with a neutralizing antibody, shows preservation of neutralization epitopes after this treatment.\nCONCLUSION: These orthogonally inactivated viral preparations with detergent resistant fractions are being explored as a novel route for safe, effective inactivated vaccines generated from a variety of enveloped viruses.",
"genre": "research_article",
"id": "sg:pub.10.1186/1743-422x-9-72",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.2343593",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2723574",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1034449",
"issn": [
"1743-422X"
],
"name": "Virology Journal",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "9"
}
],
"name": "Orthogonal inactivation of influenza and the creation of detergent resistant viral aggregates: towards a novel vaccine strategy",
"pagination": "72",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"6daea17b0cbb91865425a73b2f58e47120cdcc8526927ea9601703ff1d502097"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"22449007"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"101231645"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1186/1743-422x-9-72"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1023021965"
]
}
],
"sameAs": [
"https://doi.org/10.1186/1743-422x-9-72",
"https://app.dimensions.ai/details/publication/pub.1023021965"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T15:51",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000512.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1186%2F1743-422X-9-72"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-9-72'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-9-72'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-9-72'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-9-72'
This table displays all metadata directly associated to this object as RDF triples.
195 TRIPLES
21 PREDICATES
55 URIs
28 LITERALS
16 BLANK NODES