Deciphering the differential response of two human fibroblast cell lines following Chikungunya virus infection View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2012-12

AUTHORS

Vincent G Thon-Hon, Melanie Denizot, Ghislaine Li-Pat-Yuen, Claude Giry, Marie-Christine Jaffar-Bandjee, Philippe Gasque

ABSTRACT

BACKGROUND: Chikungunya virus (CHIKV) is an arthritogenic member of the Alphavirus genus (family Togaviridae) transmitted by Aedes mosquitoes. CHIKV is now known to target non hematopoietic cells such as epithelial, endothelial cells, fibroblasts and to less extent monocytes/macrophages. The type I interferon (IFN) response is an early innate immune mechanism that protects cells against viral infection. Cells express different pattern recognition receptors (including TLR7 and RIG-I) to sense viruses and to induce production of type I IFNs which in turn will bind to their receptor. This should result in the phosphorylation and translocation of STAT molecules into the nucleus to promote the transcription of IFN-stimulated antiviral genes (ISGs). We herein tested the capacity of CHIKV clinical isolate to infect two different human fibroblast cell lines HS 633T and HT-1080 and we analyzed the resulting type I IFN innate immune response. METHODS: Indirect immunofluorescence and quantitative RT-PCR were used to test for the susceptibility of both fibroblast cell lines to CHIKV. RESULTS: Interestingly, the two fibroblast cell lines HS 633T and HT-1080 were differently susceptible to CHIKV infection and the former producing at least 30-fold higher viral load at 48 h post-infection (PI). We found that the expression of antiviral genes (RIG-I, IFN-β, ISG54 and ISG56) was more robust in the more susceptible cell line HS 633T at 48 h PI. Moreover, CHIKV was shown to similarly interfere with the nuclear translocation of pSTAT1 in both cell lines. CONCLUSION: Critically, CHIKV can control the IFN response by preventing the nuclear translocation of pSTAT1 in both fibroblast cell lines. Counter-intuitively, the relative resistance of HT-1080 cells to CHIKV infection could not be attributed to more robust innate IFN- and ISG-dependent antiviral responses. These cell lines may prove to be valuable models to screen for novel mechanisms mobilized differentially by fibroblasts to control CHIKV infection, replication and spreading from cell to cell. More... »

PAGES

213

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1743-422x-9-213

DOI

http://dx.doi.org/10.1186/1743-422x-9-213

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027544786

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22992396


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chikungunya virus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fibroblasts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Antibody Technique, Indirect", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Profiling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Host-Pathogen Interactions", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Interferon Type I", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Real-Time Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Time Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viral Load", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Immunopathology and Infection Research Grouping (IRG), EA4517, University of La Reunion, CHU Felix Guyon and CYROI, St Denis, La Reunion, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thon-Hon", 
        "givenName": "Vincent G", 
        "id": "sg:person.01317052422.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01317052422.46"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Immunopathology and Infection Research Grouping (IRG), EA4517, University of La Reunion, CHU Felix Guyon and CYROI, St Denis, La Reunion, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Denizot", 
        "givenName": "Melanie", 
        "id": "sg:person.0677034403.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677034403.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Microbiology/Virology Laboratory, CHU Felix Guyon of La Reunion, St Denis, La Reunion, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li-Pat-Yuen", 
        "givenName": "Ghislaine", 
        "id": "sg:person.0641561522.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641561522.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Immunopathology and Infection Research Grouping (IRG), EA4517, University of La Reunion, CHU Felix Guyon and CYROI, St Denis, La Reunion, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Giry", 
        "givenName": "Claude", 
        "id": "sg:person.01155620775.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155620775.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Immunopathology and Infection Research Grouping (IRG), EA4517, University of La Reunion, CHU Felix Guyon and CYROI, St Denis, La Reunion, France", 
            "Microbiology/Virology Laboratory, CHU Felix Guyon of La Reunion, St Denis, La Reunion, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jaffar-Bandjee", 
        "givenName": "Marie-Christine", 
        "id": "sg:person.01347224447.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347224447.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Immunopathology and Infection Research Grouping (IRG), EA4517, University of La Reunion, CHU Felix Guyon and CYROI, St Denis, La Reunion, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gasque", 
        "givenName": "Philippe", 
        "id": "sg:person.01067547117.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067547117.00"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.pneurobio.2009.12.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001411961"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.00767-10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002134834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.0030089", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002222766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(88)90292-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002540515"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(91)90112-o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003171640"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.01189-10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009029252"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/17.4.1087", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009467553"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.0040029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010831245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m310616200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014460475"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.0900255", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014482043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.immuni.2005.04.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016125429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.00949-10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016531590"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.20020207", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017925643"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/605496", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018057003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/vir.0.83391-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025501586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1093616", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027055845"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.20090851", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027537782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/29.9.e45", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030834024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni1243", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031134703", 
          "https://doi.org/10.1038/ni1243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni1243", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031134703", 
          "https://doi.org/10.1038/ni1243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/590003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032684425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0952-7915(01)00305-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032699368"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1093620", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035805929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0035-9203(55)90080-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037555364"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0400937101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041795158"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1357-2725(96)00169-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047716613"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.0904181", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048746724"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.mi.42.100188.003301", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049144778"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.02411-06", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051094865"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.10-164178", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051689305"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni1112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052724075", 
          "https://doi.org/10.1038/ni1112"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni1112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052724075", 
          "https://doi.org/10.1038/ni1112"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/107999002753452674", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059207041"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/1079990041689665", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059207192"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/0022-1317-83-12-3075", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060362186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1185068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062461448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1185068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062461448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082587283", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-12", 
    "datePublishedReg": "2012-12-01", 
    "description": "BACKGROUND: Chikungunya virus (CHIKV) is an arthritogenic member of the Alphavirus genus (family Togaviridae) transmitted by Aedes mosquitoes. CHIKV is now known to target non hematopoietic cells such as epithelial, endothelial cells, fibroblasts and to less extent monocytes/macrophages. The type I interferon (IFN) response is an early innate immune mechanism that protects cells against viral infection. Cells express different pattern recognition receptors (including TLR7 and RIG-I) to sense viruses and to induce production of type I IFNs which in turn will bind to their receptor. This should result in the phosphorylation and translocation of STAT molecules into the nucleus to promote the transcription of IFN-stimulated antiviral genes (ISGs). We herein tested the capacity of CHIKV clinical isolate to infect two different human fibroblast cell lines HS 633T and HT-1080 and we analyzed the resulting type I IFN innate immune response.\nMETHODS: Indirect immunofluorescence and quantitative RT-PCR were used to test for the susceptibility of both fibroblast cell lines to CHIKV.\nRESULTS: Interestingly, the two fibroblast cell lines HS 633T and HT-1080 were differently susceptible to CHIKV infection and the former producing at least 30-fold higher viral load at 48 h post-infection (PI). We found that the expression of antiviral genes (RIG-I, IFN-\u03b2, ISG54 and ISG56) was more robust in the more susceptible cell line HS 633T at 48 h PI. Moreover, CHIKV was shown to similarly interfere with the nuclear translocation of pSTAT1 in both cell lines.\nCONCLUSION: Critically, CHIKV can control the IFN response by preventing the nuclear translocation of pSTAT1 in both fibroblast cell lines. Counter-intuitively, the relative resistance of HT-1080 cells to CHIKV infection could not be attributed to more robust innate IFN- and ISG-dependent antiviral responses. These cell lines may prove to be valuable models to screen for novel mechanisms mobilized differentially by fibroblasts to control CHIKV infection, replication and spreading from cell to cell.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1743-422x-9-213", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3781986", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1034449", 
        "issn": [
          "1743-422X"
        ], 
        "name": "Virology Journal", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Deciphering the differential response of two human fibroblast cell lines following Chikungunya virus infection", 
    "pagination": "213", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "83373da898f761a1249460c7a41546447017bfc41b4dc7ec89298630dec11d9b"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22992396"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101231645"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1743-422x-9-213"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1027544786"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1743-422x-9-213", 
      "https://app.dimensions.ai/details/publication/pub.1027544786"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:52", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000513.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1743-422X-9-213"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-9-213'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-9-213'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-9-213'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-9-213'


 

This table displays all metadata directly associated to this object as RDF triples.

265 TRIPLES      21 PREDICATES      75 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1743-422x-9-213 schema:about N0099fbada4ea4441b1341ce45b4bed25
2 N015b38727941412eb0093278d82d19ba
3 N23ceb0b2b7bf40b5abfc9b10de64b9f9
4 N28db04aeaf544bb6ac3b2cfd26b52a7d
5 N38f89302c9d245a79baa48555d28a382
6 N48b58141fdad45bcbb7b801752b43028
7 Na817bca832e042fba0ab9ce3c3ef5a36
8 Nb1279f57226d44a3ab2457bc9b37eb1c
9 Nc5f5b72ee440436c8294652e5059e94b
10 Nca36d475f0ce4caf83027e03d1e3ed9f
11 Nda4a3bd412264aa98a945b14df8a0d10
12 anzsrc-for:11
13 anzsrc-for:1107
14 schema:author Nef0a6aa993074a569ed8d7b0ffbba6fa
15 schema:citation sg:pub.10.1038/ni1112
16 sg:pub.10.1038/ni1243
17 https://app.dimensions.ai/details/publication/pub.1082587283
18 https://doi.org/10.1016/0035-9203(55)90080-8
19 https://doi.org/10.1016/0042-6822(88)90292-9
20 https://doi.org/10.1016/0042-6822(91)90112-o
21 https://doi.org/10.1016/j.immuni.2005.04.010
22 https://doi.org/10.1016/j.pneurobio.2009.12.006
23 https://doi.org/10.1016/s0952-7915(01)00305-3
24 https://doi.org/10.1016/s1357-2725(96)00169-0
25 https://doi.org/10.1073/pnas.0400937101
26 https://doi.org/10.1074/jbc.m310616200
27 https://doi.org/10.1084/jem.20020207
28 https://doi.org/10.1084/jem.20090851
29 https://doi.org/10.1086/590003
30 https://doi.org/10.1086/605496
31 https://doi.org/10.1089/107999002753452674
32 https://doi.org/10.1089/1079990041689665
33 https://doi.org/10.1093/emboj/17.4.1087
34 https://doi.org/10.1093/nar/29.9.e45
35 https://doi.org/10.1096/fj.10-164178
36 https://doi.org/10.1099/0022-1317-83-12-3075
37 https://doi.org/10.1099/vir.0.83391-0
38 https://doi.org/10.1126/science.1093616
39 https://doi.org/10.1126/science.1093620
40 https://doi.org/10.1126/science.1185068
41 https://doi.org/10.1128/jvi.00767-10
42 https://doi.org/10.1128/jvi.00949-10
43 https://doi.org/10.1128/jvi.01189-10
44 https://doi.org/10.1128/jvi.02411-06
45 https://doi.org/10.1146/annurev.mi.42.100188.003301
46 https://doi.org/10.1371/journal.ppat.0030089
47 https://doi.org/10.1371/journal.ppat.0040029
48 https://doi.org/10.4049/jimmunol.0900255
49 https://doi.org/10.4049/jimmunol.0904181
50 schema:datePublished 2012-12
51 schema:datePublishedReg 2012-12-01
52 schema:description BACKGROUND: Chikungunya virus (CHIKV) is an arthritogenic member of the Alphavirus genus (family Togaviridae) transmitted by Aedes mosquitoes. CHIKV is now known to target non hematopoietic cells such as epithelial, endothelial cells, fibroblasts and to less extent monocytes/macrophages. The type I interferon (IFN) response is an early innate immune mechanism that protects cells against viral infection. Cells express different pattern recognition receptors (including TLR7 and RIG-I) to sense viruses and to induce production of type I IFNs which in turn will bind to their receptor. This should result in the phosphorylation and translocation of STAT molecules into the nucleus to promote the transcription of IFN-stimulated antiviral genes (ISGs). We herein tested the capacity of CHIKV clinical isolate to infect two different human fibroblast cell lines HS 633T and HT-1080 and we analyzed the resulting type I IFN innate immune response. METHODS: Indirect immunofluorescence and quantitative RT-PCR were used to test for the susceptibility of both fibroblast cell lines to CHIKV. RESULTS: Interestingly, the two fibroblast cell lines HS 633T and HT-1080 were differently susceptible to CHIKV infection and the former producing at least 30-fold higher viral load at 48 h post-infection (PI). We found that the expression of antiviral genes (RIG-I, IFN-β, ISG54 and ISG56) was more robust in the more susceptible cell line HS 633T at 48 h PI. Moreover, CHIKV was shown to similarly interfere with the nuclear translocation of pSTAT1 in both cell lines. CONCLUSION: Critically, CHIKV can control the IFN response by preventing the nuclear translocation of pSTAT1 in both fibroblast cell lines. Counter-intuitively, the relative resistance of HT-1080 cells to CHIKV infection could not be attributed to more robust innate IFN- and ISG-dependent antiviral responses. These cell lines may prove to be valuable models to screen for novel mechanisms mobilized differentially by fibroblasts to control CHIKV infection, replication and spreading from cell to cell.
53 schema:genre research_article
54 schema:inLanguage en
55 schema:isAccessibleForFree true
56 schema:isPartOf Nad1370a9f1574be8a8924de66a2808d6
57 Nb0e883aedf494bedb84c71101d2cf39c
58 sg:journal.1034449
59 schema:name Deciphering the differential response of two human fibroblast cell lines following Chikungunya virus infection
60 schema:pagination 213
61 schema:productId N2149b8edf66d4d20ba3f716e01501ba7
62 N3b5daf6a4dbd42659202a2f8d0e5aaea
63 N8548610909e447f0b7a9ce76cfa1b621
64 Nbb305d7e452648aeb5725bdf910b769e
65 Nc5b4aa7cdfb148a6868cb7e46d92fb47
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027544786
67 https://doi.org/10.1186/1743-422x-9-213
68 schema:sdDatePublished 2019-04-10T15:52
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher N76feea95bc9f4eaeb02f0e7f502c4a62
71 schema:url http://link.springer.com/10.1186%2F1743-422X-9-213
72 sgo:license sg:explorer/license/
73 sgo:sdDataset articles
74 rdf:type schema:ScholarlyArticle
75 N0099fbada4ea4441b1341ce45b4bed25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Humans
77 rdf:type schema:DefinedTerm
78 N015b38727941412eb0093278d82d19ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Host-Pathogen Interactions
80 rdf:type schema:DefinedTerm
81 N2149b8edf66d4d20ba3f716e01501ba7 schema:name readcube_id
82 schema:value 83373da898f761a1249460c7a41546447017bfc41b4dc7ec89298630dec11d9b
83 rdf:type schema:PropertyValue
84 N23ceb0b2b7bf40b5abfc9b10de64b9f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Viral Load
86 rdf:type schema:DefinedTerm
87 N28db04aeaf544bb6ac3b2cfd26b52a7d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Interferon Type I
89 rdf:type schema:DefinedTerm
90 N333e5dcb8d574365bc60ffe40f6a41f5 schema:name Immunopathology and Infection Research Grouping (IRG), EA4517, University of La Reunion, CHU Felix Guyon and CYROI, St Denis, La Reunion, France
91 rdf:type schema:Organization
92 N38d01a51196346e7b32bcbcd68276771 schema:name Immunopathology and Infection Research Grouping (IRG), EA4517, University of La Reunion, CHU Felix Guyon and CYROI, St Denis, La Reunion, France
93 rdf:type schema:Organization
94 N38f89302c9d245a79baa48555d28a382 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Cell Line
96 rdf:type schema:DefinedTerm
97 N3b5daf6a4dbd42659202a2f8d0e5aaea schema:name nlm_unique_id
98 schema:value 101231645
99 rdf:type schema:PropertyValue
100 N406aa9888917401f86d93a259ed724e9 schema:name Immunopathology and Infection Research Grouping (IRG), EA4517, University of La Reunion, CHU Felix Guyon and CYROI, St Denis, La Reunion, France
101 Microbiology/Virology Laboratory, CHU Felix Guyon of La Reunion, St Denis, La Reunion, France
102 rdf:type schema:Organization
103 N48b58141fdad45bcbb7b801752b43028 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Real-Time Polymerase Chain Reaction
105 rdf:type schema:DefinedTerm
106 N59f00ab124c8470aa273784d42815ff2 schema:name Microbiology/Virology Laboratory, CHU Felix Guyon of La Reunion, St Denis, La Reunion, France
107 rdf:type schema:Organization
108 N702287eed98c455c83197d67ab94711b rdf:first sg:person.0641561522.60
109 rdf:rest Nbb2a19f16bf44dcab3e90f7567c51463
110 N76feea95bc9f4eaeb02f0e7f502c4a62 schema:name Springer Nature - SN SciGraph project
111 rdf:type schema:Organization
112 N8548610909e447f0b7a9ce76cfa1b621 schema:name dimensions_id
113 schema:value pub.1027544786
114 rdf:type schema:PropertyValue
115 Na398045a99604ee7b3b864f2c52ce2c4 schema:name Immunopathology and Infection Research Grouping (IRG), EA4517, University of La Reunion, CHU Felix Guyon and CYROI, St Denis, La Reunion, France
116 rdf:type schema:Organization
117 Na817bca832e042fba0ab9ce3c3ef5a36 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Chikungunya virus
119 rdf:type schema:DefinedTerm
120 Nad1370a9f1574be8a8924de66a2808d6 schema:issueNumber 1
121 rdf:type schema:PublicationIssue
122 Nb0e883aedf494bedb84c71101d2cf39c schema:volumeNumber 9
123 rdf:type schema:PublicationVolume
124 Nb1279f57226d44a3ab2457bc9b37eb1c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Fibroblasts
126 rdf:type schema:DefinedTerm
127 Nb15950ab05824ac682fef05880e29761 rdf:first sg:person.01347224447.67
128 rdf:rest Nd0c339b1a52543f1b290189095383a2e
129 Nba29fb4aad4b4d42a84490da3e6d7c4c rdf:first sg:person.0677034403.53
130 rdf:rest N702287eed98c455c83197d67ab94711b
131 Nbb2a19f16bf44dcab3e90f7567c51463 rdf:first sg:person.01155620775.48
132 rdf:rest Nb15950ab05824ac682fef05880e29761
133 Nbb305d7e452648aeb5725bdf910b769e schema:name pubmed_id
134 schema:value 22992396
135 rdf:type schema:PropertyValue
136 Nc5b4aa7cdfb148a6868cb7e46d92fb47 schema:name doi
137 schema:value 10.1186/1743-422x-9-213
138 rdf:type schema:PropertyValue
139 Nc5f5b72ee440436c8294652e5059e94b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Time Factors
141 rdf:type schema:DefinedTerm
142 Nca36d475f0ce4caf83027e03d1e3ed9f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Fluorescent Antibody Technique, Indirect
144 rdf:type schema:DefinedTerm
145 Nd0c339b1a52543f1b290189095383a2e rdf:first sg:person.01067547117.00
146 rdf:rest rdf:nil
147 Nd7a2a19570b24b2eb3ba6966ce59b2fc schema:name Immunopathology and Infection Research Grouping (IRG), EA4517, University of La Reunion, CHU Felix Guyon and CYROI, St Denis, La Reunion, France
148 rdf:type schema:Organization
149 Nda4a3bd412264aa98a945b14df8a0d10 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Gene Expression Profiling
151 rdf:type schema:DefinedTerm
152 Nef0a6aa993074a569ed8d7b0ffbba6fa rdf:first sg:person.01317052422.46
153 rdf:rest Nba29fb4aad4b4d42a84490da3e6d7c4c
154 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
155 schema:name Medical and Health Sciences
156 rdf:type schema:DefinedTerm
157 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
158 schema:name Immunology
159 rdf:type schema:DefinedTerm
160 sg:grant.3781986 http://pending.schema.org/fundedItem sg:pub.10.1186/1743-422x-9-213
161 rdf:type schema:MonetaryGrant
162 sg:journal.1034449 schema:issn 1743-422X
163 schema:name Virology Journal
164 rdf:type schema:Periodical
165 sg:person.01067547117.00 schema:affiliation N38d01a51196346e7b32bcbcd68276771
166 schema:familyName Gasque
167 schema:givenName Philippe
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067547117.00
169 rdf:type schema:Person
170 sg:person.01155620775.48 schema:affiliation Nd7a2a19570b24b2eb3ba6966ce59b2fc
171 schema:familyName Giry
172 schema:givenName Claude
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155620775.48
174 rdf:type schema:Person
175 sg:person.01317052422.46 schema:affiliation Na398045a99604ee7b3b864f2c52ce2c4
176 schema:familyName Thon-Hon
177 schema:givenName Vincent G
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01317052422.46
179 rdf:type schema:Person
180 sg:person.01347224447.67 schema:affiliation N406aa9888917401f86d93a259ed724e9
181 schema:familyName Jaffar-Bandjee
182 schema:givenName Marie-Christine
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347224447.67
184 rdf:type schema:Person
185 sg:person.0641561522.60 schema:affiliation N59f00ab124c8470aa273784d42815ff2
186 schema:familyName Li-Pat-Yuen
187 schema:givenName Ghislaine
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641561522.60
189 rdf:type schema:Person
190 sg:person.0677034403.53 schema:affiliation N333e5dcb8d574365bc60ffe40f6a41f5
191 schema:familyName Denizot
192 schema:givenName Melanie
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677034403.53
194 rdf:type schema:Person
195 sg:pub.10.1038/ni1112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052724075
196 https://doi.org/10.1038/ni1112
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/ni1243 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031134703
199 https://doi.org/10.1038/ni1243
200 rdf:type schema:CreativeWork
201 https://app.dimensions.ai/details/publication/pub.1082587283 schema:CreativeWork
202 https://doi.org/10.1016/0035-9203(55)90080-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037555364
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/0042-6822(88)90292-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002540515
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/0042-6822(91)90112-o schema:sameAs https://app.dimensions.ai/details/publication/pub.1003171640
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/j.immuni.2005.04.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016125429
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/j.pneurobio.2009.12.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001411961
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/s0952-7915(01)00305-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032699368
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/s1357-2725(96)00169-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047716613
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1073/pnas.0400937101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041795158
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1074/jbc.m310616200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014460475
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1084/jem.20020207 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017925643
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1084/jem.20090851 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027537782
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1086/590003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032684425
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1086/605496 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018057003
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1089/107999002753452674 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059207041
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1089/1079990041689665 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059207192
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1093/emboj/17.4.1087 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009467553
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1093/nar/29.9.e45 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030834024
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1096/fj.10-164178 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051689305
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1099/0022-1317-83-12-3075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060362186
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1099/vir.0.83391-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025501586
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1126/science.1093616 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027055845
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1126/science.1093620 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035805929
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1126/science.1185068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062461448
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1128/jvi.00767-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002134834
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1128/jvi.00949-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016531590
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1128/jvi.01189-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009029252
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1128/jvi.02411-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051094865
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1146/annurev.mi.42.100188.003301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049144778
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1371/journal.ppat.0030089 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002222766
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1371/journal.ppat.0040029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010831245
261 rdf:type schema:CreativeWork
262 https://doi.org/10.4049/jimmunol.0900255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014482043
263 rdf:type schema:CreativeWork
264 https://doi.org/10.4049/jimmunol.0904181 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048746724
265 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...