Use of a highly sensitive strand-specific quantitative PCR to identify abortive replication in the mouse model of respiratory syncytial virus ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-09-22

AUTHORS

Richard Bannister, Deborah Rodrigues, Edward J Murray, Carl Laxton, Mike Westby, Helen Bright

ABSTRACT

BACKGROUND: The BALB/c mouse is commonly used to study RSV infection and disease. However, despite the many advantages of this well-characterised model, the inoculum is large, viral replication is restricted and only a very small amount of virus can be recovered from infected animals. A key question in this model is the fate of the administered virus. Is replication really being measured or is the model measuring the survival of the virus over time? To answer these questions we developed a highly sensitive strand-specific quantitative PCR (QPCR) able to accurately quantify the amount of RSV replication in the BALB/c mouse lung, allowing characterisation of RSV negative and positive strand RNA dynamics. RESULTS: In the mouse lung, no increase in RSV genome was seen above the background of the original inoculum whilst only a limited transient increase (< 1 log) in positive strand, replicative intermediate (RI) RNA occurred. This RNA did however persist at detectable levels for 59 days post infection. As expected, ribavirin therapy reduced levels of infectious virus and RI RNA in the mouse lung. However, whilst Palivizumab therapy was also able to reduce levels of infectious virus, it failed to prevent production of intracellular RI RNA. A comparison of RSV RNA kinetics in human (A549) and mouse (KLN205) cell lines demonstrated that RSV replication was also severely delayed and impaired in vitro in the mouse cells. CONCLUSIONS: This is the first time that such a sensitive strand-specific QPCR technique has been to the RSV mouse system. We have accurately quantified the restricted and abortive nature of RSV replication in the mouse. Further in vitro studies in human and mouse cells suggest this restricted replication is due at least in part to species-specific host cell-viral interactions. More... »

PAGES

250-250

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1743-422x-7-250

DOI

http://dx.doi.org/10.1186/1743-422x-7-250

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034253108

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20860795


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lung", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred BALB C", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Respiratory Syncytial Virus Infections", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Respiratory Syncytial Virus, Human", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virus Replication", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK", 
          "id": "http://www.grid.ac/institutes/grid.418566.8", 
          "name": [
            "Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bannister", 
        "givenName": "Richard", 
        "id": "sg:person.01131425603.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131425603.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK", 
          "id": "http://www.grid.ac/institutes/grid.418566.8", 
          "name": [
            "Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rodrigues", 
        "givenName": "Deborah", 
        "id": "sg:person.0675315375.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675315375.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK", 
          "id": "http://www.grid.ac/institutes/grid.418566.8", 
          "name": [
            "Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Murray", 
        "givenName": "Edward J", 
        "id": "sg:person.01042207620.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042207620.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK", 
          "id": "http://www.grid.ac/institutes/grid.418566.8", 
          "name": [
            "Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Laxton", 
        "givenName": "Carl", 
        "id": "sg:person.01304473075.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304473075.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK", 
          "id": "http://www.grid.ac/institutes/grid.418566.8", 
          "name": [
            "Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Westby", 
        "givenName": "Mike", 
        "id": "sg:person.01315031425.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315031425.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK", 
          "id": "http://www.grid.ac/institutes/grid.418566.8", 
          "name": [
            "Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bright", 
        "givenName": "Helen", 
        "id": "sg:person.01174105575.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174105575.32"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/sj.bmt.1702078", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051935693", 
          "https://doi.org/10.1038/sj.bmt.1702078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1743-422x-2-46", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035068607", 
          "https://doi.org/10.1186/1743-422x-2-46"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2010-09-22", 
    "datePublishedReg": "2010-09-22", 
    "description": "BACKGROUND: The BALB/c mouse is commonly used to study RSV infection and disease. However, despite the many advantages of this well-characterised model, the inoculum is large, viral replication is restricted and only a very small amount of virus can be recovered from infected animals. A key question in this model is the fate of the administered virus. Is replication really being measured or is the model measuring the survival of the virus over time? To answer these questions we developed a highly sensitive strand-specific quantitative PCR (QPCR) able to accurately quantify the amount of RSV replication in the BALB/c mouse lung, allowing characterisation of RSV negative and positive strand RNA dynamics.\nRESULTS: In the mouse lung, no increase in RSV genome was seen above the background of the original inoculum whilst only a limited transient increase (< 1 log) in positive strand, replicative intermediate (RI) RNA occurred. This RNA did however persist at detectable levels for 59 days post infection. As expected, ribavirin therapy reduced levels of infectious virus and RI RNA in the mouse lung. However, whilst Palivizumab therapy was also able to reduce levels of infectious virus, it failed to prevent production of intracellular RI RNA. A comparison of RSV RNA kinetics in human (A549) and mouse (KLN205) cell lines demonstrated that RSV replication was also severely delayed and impaired in vitro in the mouse cells.\nCONCLUSIONS: This is the first time that such a sensitive strand-specific QPCR technique has been to the RSV mouse system. We have accurately quantified the restricted and abortive nature of RSV replication in the mouse. Further in vitro studies in human and mouse cells suggest this restricted replication is due at least in part to species-specific host cell-viral interactions.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/1743-422x-7-250", 
    "inLanguage": "en", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1034449", 
        "issn": [
          "1743-422X"
        ], 
        "name": "Virology Journal", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "keywords": [
      "RSV replication", 
      "mouse lung", 
      "infectious virus", 
      "respiratory syncytial virus disease", 
      "BALB/c mouse lung", 
      "BALB/c mice", 
      "quantitative PCR", 
      "days post infection", 
      "ribavirin therapy", 
      "palivizumab therapy", 
      "RSV infection", 
      "mouse model", 
      "post infection", 
      "viral replication", 
      "infected animals", 
      "RNA kinetics", 
      "transient increase", 
      "lung", 
      "abortive replication", 
      "mouse system", 
      "mouse cells", 
      "virus", 
      "RSV genome", 
      "RI RNA", 
      "virus disease", 
      "cell lines", 
      "therapy", 
      "detectable levels", 
      "infection", 
      "disease", 
      "mice", 
      "abortive nature", 
      "original inoculum", 
      "mouse cell lines", 
      "PCR", 
      "qPCR technique", 
      "replicative intermediate RNA", 
      "RNA", 
      "cells", 
      "humans", 
      "replication", 
      "levels", 
      "RSV", 
      "positive strand", 
      "survival", 
      "animals", 
      "increase", 
      "intermediate RNA", 
      "inoculum", 
      "study", 
      "background", 
      "time", 
      "first time", 
      "key questions", 
      "use", 
      "small amount", 
      "amount", 
      "questions", 
      "lines", 
      "model", 
      "comparison", 
      "production", 
      "fate", 
      "part", 
      "technique", 
      "interaction", 
      "genome", 
      "system", 
      "kinetics", 
      "advantages", 
      "nature", 
      "RNA dynamics", 
      "characterisation", 
      "strands", 
      "dynamics", 
      "c mouse", 
      "sensitive strand-specific quantitative PCR", 
      "strand-specific quantitative PCR", 
      "c mouse lung", 
      "characterisation of RSV", 
      "positive strand RNA dynamics", 
      "strand RNA dynamics", 
      "limited transient increase", 
      "intracellular RI RNA", 
      "RSV RNA kinetics", 
      "sensitive strand-specific QPCR technique", 
      "strand-specific QPCR technique", 
      "RSV mouse system", 
      "species-specific host cell-viral interactions", 
      "host cell-viral interactions", 
      "cell-viral interactions", 
      "syncytial virus disease"
    ], 
    "name": "Use of a highly sensitive strand-specific quantitative PCR to identify abortive replication in the mouse model of respiratory syncytial virus disease", 
    "pagination": "250-250", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1034253108"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1743-422x-7-250"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "20860795"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1743-422x-7-250", 
      "https://app.dimensions.ai/details/publication/pub.1034253108"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:21", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_504.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/1743-422x-7-250"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-7-250'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-7-250'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-7-250'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-7-250'


 

This table displays all metadata directly associated to this object as RDF triples.

244 TRIPLES      22 PREDICATES      132 URIs      122 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1743-422x-7-250 schema:about N02c353f49ef242c3990036e3131eebb3
2 N1895d79c8728416fb77abba55f0c547a
3 N1cebbc80cc464813bf14dbb74bfafeb4
4 N3279a380d5e54e568cdd4348f7f518b9
5 N6019dc7647ec44f0ae23750c1534bb0b
6 N8255df65aad74d3794b0184e9b3e3e8b
7 Na0c0b94f76294b25b5a5770555350993
8 Na1597c94a57047418450b82715653229
9 Na61e378bfad2483c93c7947ffa4483a5
10 Na65232050ef6446fbfc35cc2b13f9cd5
11 Nc854af652ea443a7a64891572f36290a
12 Nf6108cd377da4466a68d393baa3af88c
13 anzsrc-for:11
14 anzsrc-for:1108
15 schema:author N9bdf898f906640ce8dd8e2b3856efff7
16 schema:citation sg:pub.10.1038/sj.bmt.1702078
17 sg:pub.10.1186/1743-422x-2-46
18 schema:datePublished 2010-09-22
19 schema:datePublishedReg 2010-09-22
20 schema:description BACKGROUND: The BALB/c mouse is commonly used to study RSV infection and disease. However, despite the many advantages of this well-characterised model, the inoculum is large, viral replication is restricted and only a very small amount of virus can be recovered from infected animals. A key question in this model is the fate of the administered virus. Is replication really being measured or is the model measuring the survival of the virus over time? To answer these questions we developed a highly sensitive strand-specific quantitative PCR (QPCR) able to accurately quantify the amount of RSV replication in the BALB/c mouse lung, allowing characterisation of RSV negative and positive strand RNA dynamics. RESULTS: In the mouse lung, no increase in RSV genome was seen above the background of the original inoculum whilst only a limited transient increase (< 1 log) in positive strand, replicative intermediate (RI) RNA occurred. This RNA did however persist at detectable levels for 59 days post infection. As expected, ribavirin therapy reduced levels of infectious virus and RI RNA in the mouse lung. However, whilst Palivizumab therapy was also able to reduce levels of infectious virus, it failed to prevent production of intracellular RI RNA. A comparison of RSV RNA kinetics in human (A549) and mouse (KLN205) cell lines demonstrated that RSV replication was also severely delayed and impaired in vitro in the mouse cells. CONCLUSIONS: This is the first time that such a sensitive strand-specific QPCR technique has been to the RSV mouse system. We have accurately quantified the restricted and abortive nature of RSV replication in the mouse. Further in vitro studies in human and mouse cells suggest this restricted replication is due at least in part to species-specific host cell-viral interactions.
21 schema:genre article
22 schema:inLanguage en
23 schema:isAccessibleForFree true
24 schema:isPartOf N2251f26a375046a8b4307dd78e179aa6
25 N60846ebc284243549a9dd6a4b247f5bd
26 sg:journal.1034449
27 schema:keywords BALB/c mice
28 BALB/c mouse lung
29 PCR
30 RI RNA
31 RNA
32 RNA dynamics
33 RNA kinetics
34 RSV
35 RSV RNA kinetics
36 RSV genome
37 RSV infection
38 RSV mouse system
39 RSV replication
40 abortive nature
41 abortive replication
42 advantages
43 amount
44 animals
45 background
46 c mouse
47 c mouse lung
48 cell lines
49 cell-viral interactions
50 cells
51 characterisation
52 characterisation of RSV
53 comparison
54 days post infection
55 detectable levels
56 disease
57 dynamics
58 fate
59 first time
60 genome
61 host cell-viral interactions
62 humans
63 increase
64 infected animals
65 infection
66 infectious virus
67 inoculum
68 interaction
69 intermediate RNA
70 intracellular RI RNA
71 key questions
72 kinetics
73 levels
74 limited transient increase
75 lines
76 lung
77 mice
78 model
79 mouse cell lines
80 mouse cells
81 mouse lung
82 mouse model
83 mouse system
84 nature
85 original inoculum
86 palivizumab therapy
87 part
88 positive strand
89 positive strand RNA dynamics
90 post infection
91 production
92 qPCR technique
93 quantitative PCR
94 questions
95 replication
96 replicative intermediate RNA
97 respiratory syncytial virus disease
98 ribavirin therapy
99 sensitive strand-specific QPCR technique
100 sensitive strand-specific quantitative PCR
101 small amount
102 species-specific host cell-viral interactions
103 strand RNA dynamics
104 strand-specific QPCR technique
105 strand-specific quantitative PCR
106 strands
107 study
108 survival
109 syncytial virus disease
110 system
111 technique
112 therapy
113 time
114 transient increase
115 use
116 viral replication
117 virus
118 virus disease
119 schema:name Use of a highly sensitive strand-specific quantitative PCR to identify abortive replication in the mouse model of respiratory syncytial virus disease
120 schema:pagination 250-250
121 schema:productId N07563b1556af401e9856f79e99ddc51e
122 N31503e8321dd46359b6795fc809417f9
123 Nf771df6a92fd4ac489556653095b86ab
124 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034253108
125 https://doi.org/10.1186/1743-422x-7-250
126 schema:sdDatePublished 2022-01-01T18:21
127 schema:sdLicense https://scigraph.springernature.com/explorer/license/
128 schema:sdPublisher Nfc6107f75ce840eabdedba020a9c4642
129 schema:url https://doi.org/10.1186/1743-422x-7-250
130 sgo:license sg:explorer/license/
131 sgo:sdDataset articles
132 rdf:type schema:ScholarlyArticle
133 N02c353f49ef242c3990036e3131eebb3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Mice
135 rdf:type schema:DefinedTerm
136 N05301caa49704eb39b8eff6caf642059 rdf:first sg:person.01315031425.05
137 rdf:rest N724014eb922348c7ab65a392c3e834a8
138 N07563b1556af401e9856f79e99ddc51e schema:name dimensions_id
139 schema:value pub.1034253108
140 rdf:type schema:PropertyValue
141 N1895d79c8728416fb77abba55f0c547a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Lung
143 rdf:type schema:DefinedTerm
144 N1cebbc80cc464813bf14dbb74bfafeb4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Humans
146 rdf:type schema:DefinedTerm
147 N2251f26a375046a8b4307dd78e179aa6 schema:volumeNumber 7
148 rdf:type schema:PublicationVolume
149 N31503e8321dd46359b6795fc809417f9 schema:name doi
150 schema:value 10.1186/1743-422x-7-250
151 rdf:type schema:PropertyValue
152 N3279a380d5e54e568cdd4348f7f518b9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Polymerase Chain Reaction
154 rdf:type schema:DefinedTerm
155 N6019dc7647ec44f0ae23750c1534bb0b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Disease Models, Animal
157 rdf:type schema:DefinedTerm
158 N60846ebc284243549a9dd6a4b247f5bd schema:issueNumber 1
159 rdf:type schema:PublicationIssue
160 N724014eb922348c7ab65a392c3e834a8 rdf:first sg:person.01174105575.32
161 rdf:rest rdf:nil
162 N8255df65aad74d3794b0184e9b3e3e8b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Respiratory Syncytial Virus Infections
164 rdf:type schema:DefinedTerm
165 N9bdf898f906640ce8dd8e2b3856efff7 rdf:first sg:person.01131425603.56
166 rdf:rest Nf5a1c2db3cfa4f89994cf3503ca2c822
167 Na0c0b94f76294b25b5a5770555350993 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name RNA, Viral
169 rdf:type schema:DefinedTerm
170 Na1597c94a57047418450b82715653229 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Female
172 rdf:type schema:DefinedTerm
173 Na61e378bfad2483c93c7947ffa4483a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
174 schema:name Animals
175 rdf:type schema:DefinedTerm
176 Na65232050ef6446fbfc35cc2b13f9cd5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
177 schema:name Respiratory Syncytial Virus, Human
178 rdf:type schema:DefinedTerm
179 Nc4d1e5467e8140c79bcc682aac1becac rdf:first sg:person.01042207620.34
180 rdf:rest Nf177544ec738499ba9819546e255bfeb
181 Nc854af652ea443a7a64891572f36290a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
182 schema:name Mice, Inbred BALB C
183 rdf:type schema:DefinedTerm
184 Nf177544ec738499ba9819546e255bfeb rdf:first sg:person.01304473075.24
185 rdf:rest N05301caa49704eb39b8eff6caf642059
186 Nf5a1c2db3cfa4f89994cf3503ca2c822 rdf:first sg:person.0675315375.13
187 rdf:rest Nc4d1e5467e8140c79bcc682aac1becac
188 Nf6108cd377da4466a68d393baa3af88c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Virus Replication
190 rdf:type schema:DefinedTerm
191 Nf771df6a92fd4ac489556653095b86ab schema:name pubmed_id
192 schema:value 20860795
193 rdf:type schema:PropertyValue
194 Nfc6107f75ce840eabdedba020a9c4642 schema:name Springer Nature - SN SciGraph project
195 rdf:type schema:Organization
196 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
197 schema:name Medical and Health Sciences
198 rdf:type schema:DefinedTerm
199 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
200 schema:name Medical Microbiology
201 rdf:type schema:DefinedTerm
202 sg:journal.1034449 schema:issn 1743-422X
203 schema:name Virology Journal
204 schema:publisher Springer Nature
205 rdf:type schema:Periodical
206 sg:person.01042207620.34 schema:affiliation grid-institutes:grid.418566.8
207 schema:familyName Murray
208 schema:givenName Edward J
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042207620.34
210 rdf:type schema:Person
211 sg:person.01131425603.56 schema:affiliation grid-institutes:grid.418566.8
212 schema:familyName Bannister
213 schema:givenName Richard
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131425603.56
215 rdf:type schema:Person
216 sg:person.01174105575.32 schema:affiliation grid-institutes:grid.418566.8
217 schema:familyName Bright
218 schema:givenName Helen
219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174105575.32
220 rdf:type schema:Person
221 sg:person.01304473075.24 schema:affiliation grid-institutes:grid.418566.8
222 schema:familyName Laxton
223 schema:givenName Carl
224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304473075.24
225 rdf:type schema:Person
226 sg:person.01315031425.05 schema:affiliation grid-institutes:grid.418566.8
227 schema:familyName Westby
228 schema:givenName Mike
229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315031425.05
230 rdf:type schema:Person
231 sg:person.0675315375.13 schema:affiliation grid-institutes:grid.418566.8
232 schema:familyName Rodrigues
233 schema:givenName Deborah
234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675315375.13
235 rdf:type schema:Person
236 sg:pub.10.1038/sj.bmt.1702078 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051935693
237 https://doi.org/10.1038/sj.bmt.1702078
238 rdf:type schema:CreativeWork
239 sg:pub.10.1186/1743-422x-2-46 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035068607
240 https://doi.org/10.1186/1743-422x-2-46
241 rdf:type schema:CreativeWork
242 grid-institutes:grid.418566.8 schema:alternateName Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK
243 schema:name Infectious Diseases Group, Pfizer Global Research and Development, Sandwich, Kent, CT13 9NJ, UK
244 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...