Detection of dengue virus in saliva and urine by real time RT-PCR View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-12

AUTHORS

Telma R Poloni, Anibal S Oliveira, Helda L Alfonso, Larissa R Galvão, Alberto A Amarilla, Dimair F Poloni, Luiz T Figueiredo, Victor H Aquino

ABSTRACT

Early diagnosis of dengue virus (DENV) infection is important for patient management and control of dengue outbreaks. The objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of DENV infection by real time RT-PCR. Two febrile patients, who have been attended at the General Hospital of the School of Medicine of Ribeirao Preto, Sao Paulo University were included in the study. Serum, urine and saliva samples collected from both patients were subjected to real time RT-PCR for DENV detection and quantification. Dengue RNA was detected in serum, urine and saliva samples of both patients. Patient 1 was infected with DENV-2 and patient 2 with DENV-3. Data presented in this study suggest that urine and saliva could be used as alternative samples for early diagnosis of dengue virus infection when blood samples are difficult to obtain, e.g., in newborns and patients with hemorrhagic syndromes. More... »

PAGES

22

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1743-422x-7-22

DOI

http://dx.doi.org/10.1186/1743-422x-7-22

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029351058

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20105295


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