Caveolin-1 interacts with the Gag precursor of murine leukaemia virus and modulates virus production View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-12

AUTHORS

Zheng Yu, Christiane Beer, Mario Koester, Manfred Wirth

ABSTRACT

BACKGROUND: Retroviral Gag determines virus assembly at the plasma membrane and the formation of virus-like particles in intracellular multivesicular bodies. Thereby, retroviruses exploit by interaction with cellular partners the cellular machineries for vesicular transport in various ways. RESULTS: The retroviral Gag precursor protein drives assembly of murine leukaemia viruses (MLV) at the plasma membrane (PM) and the formation of virus like particles in multivesicular bodies (MVBs). In our study we show that caveolin-1 (Cav-1), a multifunctional membrane-associated protein, co-localizes with Gag in a punctate pattern at the PM of infected NIH 3T3 cells. We provide evidence that Cav-1 interacts with the matrix protein (MA) of the Gag precursor. This interaction is mediated by a Cav-1 binding domain (CBD) within the N-terminus of MA. Interestingly, the CBD motif identified within MA is highly conserved among most other gamma-retroviruses. Furthermore, Cav-1 is incorporated into MLV released from NIH 3T3 cells. Overexpression of a GFP fusion protein containing the putative CBD of the retroviral MA resulted in a considerable decrease in production of infectious retrovirus. Moreover, expression of a dominant-negative Cav-1 mutant affected retroviral titres significantly. CONCLUSION: This study demonstrates that Cav-1 interacts with MLV Gag, co-localizes with Gag at the PM and affects the production of infectious virus. The results strongly suggest a role for Cav-1 in the process of virus assembly. More... »

PAGES

73

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/1743-422x-3-73

DOI

http://dx.doi.org/10.1186/1743-422x-3-73

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037117787

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16956408


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Caveolin 1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Membrane", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Products, gag", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leukemia Virus, Murine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "NIH 3T3 Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virus Replication", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "GBF German Research Centre for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.418123.d", 
          "name": [
            "Molecular Biotechnology Division, German Research Centre for Biotechnology, Mascheroder Weg 1, Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yu", 
        "givenName": "Zheng", 
        "id": "sg:person.0577656341.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0577656341.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Aarhus University", 
          "id": "https://www.grid.ac/institutes/grid.7048.b", 
          "name": [
            "Molecular Biotechnology Division, German Research Centre for Biotechnology, Mascheroder Weg 1, Braunschweig, Germany", 
            "Department of Molecular Biology,, Aarhus University, C.F. Mollers Alle 130, Aarhus, Denmark"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Beer", 
        "givenName": "Christiane", 
        "id": "sg:person.0777366104.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777366104.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "GBF German Research Centre for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.418123.d", 
          "name": [
            "Molecular Biotechnology Division, German Research Centre for Biotechnology, Mascheroder Weg 1, Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koester", 
        "givenName": "Mario", 
        "id": "sg:person.0576057663.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576057663.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "GBF German Research Centre for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.418123.d", 
          "name": [
            "Molecular Biotechnology Division, German Research Centre for Biotechnology, Mascheroder Weg 1, Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wirth", 
        "givenName": "Manfred", 
        "id": "sg:person.01057172347.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057172347.18"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1074/jbc.m311945200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001638927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.274.6.3910", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002755975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.135.6.1841", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004320595"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.virol.2004.07.029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004388642"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/viro.1994.1650", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006665271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0962-8924(02)02402-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007978068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0962-8924(02)02402-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007978068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.83.19.7246", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009672831"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m413735200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009798273"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m413735200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009798273"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1743-422x-2-36", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010865847", 
          "https://doi.org/10.1186/1743-422x-2-36"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.immuni.2004.08.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013820364"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.77.21.11651-11660.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015897098"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.16.9690", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017046643"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35074539", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017645872", 
          "https://doi.org/10.1038/35074539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35074539", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017645872", 
          "https://doi.org/10.1038/35074539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.74.23.11222-11229.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017732630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.00117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019367905"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.76.23.11845-11852.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020073616"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.12.11.3601", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029448932"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(99)00164-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030649135"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.semcdb.2005.01.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034699827"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.110.3.637", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035495250"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.75.15.7175-7183.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039105271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.78.13.7153-7164.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039916283"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1194/jlr.m200449-jlr200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042604374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.10.6525", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043381192"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.10.6525", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043381192"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1034/j.1600-0854.2003.00135.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044183105"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m003401200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048891572"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.46.29182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049742330"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.46.29182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049742330"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.virusres.2004.08.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050220464"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1210/mend.13.12.0392", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064331759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077300176", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078304225", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079168869", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082392982", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082630437", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082669062", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082769487", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082903933", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083104029", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-12", 
    "datePublishedReg": "2006-12-01", 
    "description": "BACKGROUND: Retroviral Gag determines virus assembly at the plasma membrane and the formation of virus-like particles in intracellular multivesicular bodies. Thereby, retroviruses exploit by interaction with cellular partners the cellular machineries for vesicular transport in various ways.\nRESULTS: The retroviral Gag precursor protein drives assembly of murine leukaemia viruses (MLV) at the plasma membrane (PM) and the formation of virus like particles in multivesicular bodies (MVBs). In our study we show that caveolin-1 (Cav-1), a multifunctional membrane-associated protein, co-localizes with Gag in a punctate pattern at the PM of infected NIH 3T3 cells. We provide evidence that Cav-1 interacts with the matrix protein (MA) of the Gag precursor. This interaction is mediated by a Cav-1 binding domain (CBD) within the N-terminus of MA. Interestingly, the CBD motif identified within MA is highly conserved among most other gamma-retroviruses. Furthermore, Cav-1 is incorporated into MLV released from NIH 3T3 cells. Overexpression of a GFP fusion protein containing the putative CBD of the retroviral MA resulted in a considerable decrease in production of infectious retrovirus. Moreover, expression of a dominant-negative Cav-1 mutant affected retroviral titres significantly.\nCONCLUSION: This study demonstrates that Cav-1 interacts with MLV Gag, co-localizes with Gag at the PM and affects the production of infectious virus. The results strongly suggest a role for Cav-1 in the process of virus assembly.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/1743-422x-3-73", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1034449", 
        "issn": [
          "1743-422X"
        ], 
        "name": "Virology Journal", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "3"
      }
    ], 
    "name": "Caveolin-1 interacts with the Gag precursor of murine leukaemia virus and modulates virus production", 
    "pagination": "73", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b0d11e1d91a5a87adf1f8e749aff6ab6a7b6030e5827145f6c4082838c72e45a"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16956408"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101231645"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/1743-422x-3-73"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1037117787"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/1743-422x-3-73", 
      "https://app.dimensions.ai/details/publication/pub.1037117787"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T16:43", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000514.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2F1743-422X-3-73"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-3-73'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-3-73'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-3-73'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/1743-422x-3-73'


 

This table displays all metadata directly associated to this object as RDF triples.

232 TRIPLES      21 PREDICATES      75 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/1743-422x-3-73 schema:about N5b99c8fb496f462ebec612970dc12e58
2 N6f4dae7c69714d078ef42de6aa9792fe
3 N712eb42edc1c4b41b51f58152f997eac
4 N7ac4cad65b534175a007023e07ff6cb8
5 N7ba30acce0f24b77946b5d715ae9b0f4
6 N7e6ad0535f4245ffb5dbefdb9cea4616
7 Na5825e3436ab436780f9157eccacdcb1
8 Nf796605409074ad790709329fec191d3
9 anzsrc-for:06
10 anzsrc-for:0601
11 schema:author N2955de3a493345da8e3b5f34bfd4a7ab
12 schema:citation sg:pub.10.1038/35074539
13 sg:pub.10.1186/1743-422x-2-36
14 https://app.dimensions.ai/details/publication/pub.1077300176
15 https://app.dimensions.ai/details/publication/pub.1078304225
16 https://app.dimensions.ai/details/publication/pub.1079168869
17 https://app.dimensions.ai/details/publication/pub.1082392982
18 https://app.dimensions.ai/details/publication/pub.1082630437
19 https://app.dimensions.ai/details/publication/pub.1082669062
20 https://app.dimensions.ai/details/publication/pub.1082769487
21 https://app.dimensions.ai/details/publication/pub.1082903933
22 https://app.dimensions.ai/details/publication/pub.1083104029
23 https://doi.org/10.1006/viro.1994.1650
24 https://doi.org/10.1016/j.immuni.2004.08.015
25 https://doi.org/10.1016/j.semcdb.2005.01.007
26 https://doi.org/10.1016/j.virol.2004.07.029
27 https://doi.org/10.1016/j.virusres.2004.08.007
28 https://doi.org/10.1016/s0014-5793(99)00164-7
29 https://doi.org/10.1016/s0962-8924(02)02402-9
30 https://doi.org/10.1034/j.1600-0854.2003.00135.x
31 https://doi.org/10.1073/pnas.83.19.7246
32 https://doi.org/10.1074/jbc.271.16.9690
33 https://doi.org/10.1074/jbc.271.46.29182
34 https://doi.org/10.1074/jbc.272.10.6525
35 https://doi.org/10.1074/jbc.274.6.3910
36 https://doi.org/10.1074/jbc.m003401200
37 https://doi.org/10.1074/jbc.m311945200
38 https://doi.org/10.1074/jbc.m413735200
39 https://doi.org/10.1083/jcb.110.3.637
40 https://doi.org/10.1083/jcb.135.6.1841
41 https://doi.org/10.1091/mbc.12.11.3601
42 https://doi.org/10.1128/jvi.74.23.11222-11229.2000
43 https://doi.org/10.1128/jvi.75.15.7175-7183.2001
44 https://doi.org/10.1128/jvi.76.23.11845-11852.2002
45 https://doi.org/10.1128/jvi.77.21.11651-11660.2003
46 https://doi.org/10.1128/jvi.78.13.7153-7164.2004
47 https://doi.org/10.1194/jlr.m200449-jlr200
48 https://doi.org/10.1210/mend.13.12.0392
49 https://doi.org/10.1242/jcs.00117
50 schema:datePublished 2006-12
51 schema:datePublishedReg 2006-12-01
52 schema:description BACKGROUND: Retroviral Gag determines virus assembly at the plasma membrane and the formation of virus-like particles in intracellular multivesicular bodies. Thereby, retroviruses exploit by interaction with cellular partners the cellular machineries for vesicular transport in various ways. RESULTS: The retroviral Gag precursor protein drives assembly of murine leukaemia viruses (MLV) at the plasma membrane (PM) and the formation of virus like particles in multivesicular bodies (MVBs). In our study we show that caveolin-1 (Cav-1), a multifunctional membrane-associated protein, co-localizes with Gag in a punctate pattern at the PM of infected NIH 3T3 cells. We provide evidence that Cav-1 interacts with the matrix protein (MA) of the Gag precursor. This interaction is mediated by a Cav-1 binding domain (CBD) within the N-terminus of MA. Interestingly, the CBD motif identified within MA is highly conserved among most other gamma-retroviruses. Furthermore, Cav-1 is incorporated into MLV released from NIH 3T3 cells. Overexpression of a GFP fusion protein containing the putative CBD of the retroviral MA resulted in a considerable decrease in production of infectious retrovirus. Moreover, expression of a dominant-negative Cav-1 mutant affected retroviral titres significantly. CONCLUSION: This study demonstrates that Cav-1 interacts with MLV Gag, co-localizes with Gag at the PM and affects the production of infectious virus. The results strongly suggest a role for Cav-1 in the process of virus assembly.
53 schema:genre research_article
54 schema:inLanguage en
55 schema:isAccessibleForFree true
56 schema:isPartOf N34b438f132e743d4b80b09d80baf1f83
57 Ne1c4160e2acc4c69939a6606678e6d6f
58 sg:journal.1034449
59 schema:name Caveolin-1 interacts with the Gag precursor of murine leukaemia virus and modulates virus production
60 schema:pagination 73
61 schema:productId N3334c5cf26bb469bad9a159e1ec9ad3b
62 N750524ce379e4893b5b779db6016cb3c
63 N97ec63d056b34a99b45700f850bfd5af
64 Nadb17336d2b44bffb15db6d4fd647ec5
65 Nf7cb07727b1d427a8451b6a89d4a8322
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037117787
67 https://doi.org/10.1186/1743-422x-3-73
68 schema:sdDatePublished 2019-04-10T16:43
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher N03a9b5bd868c4a35857bcb07a4e0d530
71 schema:url http://link.springer.com/10.1186%2F1743-422X-3-73
72 sgo:license sg:explorer/license/
73 sgo:sdDataset articles
74 rdf:type schema:ScholarlyArticle
75 N03a9b5bd868c4a35857bcb07a4e0d530 schema:name Springer Nature - SN SciGraph project
76 rdf:type schema:Organization
77 N2955de3a493345da8e3b5f34bfd4a7ab rdf:first sg:person.0577656341.54
78 rdf:rest N6dba03591f70417587738f9bbe80ccf6
79 N3334c5cf26bb469bad9a159e1ec9ad3b schema:name readcube_id
80 schema:value b0d11e1d91a5a87adf1f8e749aff6ab6a7b6030e5827145f6c4082838c72e45a
81 rdf:type schema:PropertyValue
82 N34b438f132e743d4b80b09d80baf1f83 schema:volumeNumber 3
83 rdf:type schema:PublicationVolume
84 N5b99c8fb496f462ebec612970dc12e58 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Mice
86 rdf:type schema:DefinedTerm
87 N6dba03591f70417587738f9bbe80ccf6 rdf:first sg:person.0777366104.54
88 rdf:rest N839cf95746774422bbb4d05e2df6d3b7
89 N6f4dae7c69714d078ef42de6aa9792fe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name NIH 3T3 Cells
91 rdf:type schema:DefinedTerm
92 N712eb42edc1c4b41b51f58152f997eac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Gene Products, gag
94 rdf:type schema:DefinedTerm
95 N750524ce379e4893b5b779db6016cb3c schema:name pubmed_id
96 schema:value 16956408
97 rdf:type schema:PropertyValue
98 N7ac4cad65b534175a007023e07ff6cb8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Virus Replication
100 rdf:type schema:DefinedTerm
101 N7ba30acce0f24b77946b5d715ae9b0f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Animals
103 rdf:type schema:DefinedTerm
104 N7e6ad0535f4245ffb5dbefdb9cea4616 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Caveolin 1
106 rdf:type schema:DefinedTerm
107 N839cf95746774422bbb4d05e2df6d3b7 rdf:first sg:person.0576057663.54
108 rdf:rest Na6be09590389428ca9ed4a60d87fe1b0
109 N97ec63d056b34a99b45700f850bfd5af schema:name doi
110 schema:value 10.1186/1743-422x-3-73
111 rdf:type schema:PropertyValue
112 Na5825e3436ab436780f9157eccacdcb1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Leukemia Virus, Murine
114 rdf:type schema:DefinedTerm
115 Na6be09590389428ca9ed4a60d87fe1b0 rdf:first sg:person.01057172347.18
116 rdf:rest rdf:nil
117 Nadb17336d2b44bffb15db6d4fd647ec5 schema:name nlm_unique_id
118 schema:value 101231645
119 rdf:type schema:PropertyValue
120 Ne1c4160e2acc4c69939a6606678e6d6f schema:issueNumber 1
121 rdf:type schema:PublicationIssue
122 Nf796605409074ad790709329fec191d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Cell Membrane
124 rdf:type schema:DefinedTerm
125 Nf7cb07727b1d427a8451b6a89d4a8322 schema:name dimensions_id
126 schema:value pub.1037117787
127 rdf:type schema:PropertyValue
128 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
129 schema:name Biological Sciences
130 rdf:type schema:DefinedTerm
131 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
132 schema:name Biochemistry and Cell Biology
133 rdf:type schema:DefinedTerm
134 sg:journal.1034449 schema:issn 1743-422X
135 schema:name Virology Journal
136 rdf:type schema:Periodical
137 sg:person.01057172347.18 schema:affiliation https://www.grid.ac/institutes/grid.418123.d
138 schema:familyName Wirth
139 schema:givenName Manfred
140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057172347.18
141 rdf:type schema:Person
142 sg:person.0576057663.54 schema:affiliation https://www.grid.ac/institutes/grid.418123.d
143 schema:familyName Koester
144 schema:givenName Mario
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576057663.54
146 rdf:type schema:Person
147 sg:person.0577656341.54 schema:affiliation https://www.grid.ac/institutes/grid.418123.d
148 schema:familyName Yu
149 schema:givenName Zheng
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0577656341.54
151 rdf:type schema:Person
152 sg:person.0777366104.54 schema:affiliation https://www.grid.ac/institutes/grid.7048.b
153 schema:familyName Beer
154 schema:givenName Christiane
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777366104.54
156 rdf:type schema:Person
157 sg:pub.10.1038/35074539 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017645872
158 https://doi.org/10.1038/35074539
159 rdf:type schema:CreativeWork
160 sg:pub.10.1186/1743-422x-2-36 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010865847
161 https://doi.org/10.1186/1743-422x-2-36
162 rdf:type schema:CreativeWork
163 https://app.dimensions.ai/details/publication/pub.1077300176 schema:CreativeWork
164 https://app.dimensions.ai/details/publication/pub.1078304225 schema:CreativeWork
165 https://app.dimensions.ai/details/publication/pub.1079168869 schema:CreativeWork
166 https://app.dimensions.ai/details/publication/pub.1082392982 schema:CreativeWork
167 https://app.dimensions.ai/details/publication/pub.1082630437 schema:CreativeWork
168 https://app.dimensions.ai/details/publication/pub.1082669062 schema:CreativeWork
169 https://app.dimensions.ai/details/publication/pub.1082769487 schema:CreativeWork
170 https://app.dimensions.ai/details/publication/pub.1082903933 schema:CreativeWork
171 https://app.dimensions.ai/details/publication/pub.1083104029 schema:CreativeWork
172 https://doi.org/10.1006/viro.1994.1650 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006665271
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1016/j.immuni.2004.08.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013820364
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1016/j.semcdb.2005.01.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034699827
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1016/j.virol.2004.07.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004388642
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1016/j.virusres.2004.08.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050220464
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1016/s0014-5793(99)00164-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030649135
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1016/s0962-8924(02)02402-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007978068
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1034/j.1600-0854.2003.00135.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044183105
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1073/pnas.83.19.7246 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009672831
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1074/jbc.271.16.9690 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017046643
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1074/jbc.271.46.29182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049742330
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1074/jbc.272.10.6525 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043381192
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1074/jbc.274.6.3910 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002755975
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1074/jbc.m003401200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048891572
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1074/jbc.m311945200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001638927
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1074/jbc.m413735200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009798273
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1083/jcb.110.3.637 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035495250
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1083/jcb.135.6.1841 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004320595
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1091/mbc.12.11.3601 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029448932
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1128/jvi.74.23.11222-11229.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017732630
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1128/jvi.75.15.7175-7183.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039105271
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1128/jvi.76.23.11845-11852.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020073616
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1128/jvi.77.21.11651-11660.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015897098
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1128/jvi.78.13.7153-7164.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039916283
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1194/jlr.m200449-jlr200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042604374
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1210/mend.13.12.0392 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064331759
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1242/jcs.00117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019367905
225 rdf:type schema:CreativeWork
226 https://www.grid.ac/institutes/grid.418123.d schema:alternateName GBF German Research Centre for Biotechnology
227 schema:name Molecular Biotechnology Division, German Research Centre for Biotechnology, Mascheroder Weg 1, Braunschweig, Germany
228 rdf:type schema:Organization
229 https://www.grid.ac/institutes/grid.7048.b schema:alternateName Aarhus University
230 schema:name Department of Molecular Biology,, Aarhus University, C.F. Mollers Alle 130, Aarhus, Denmark
231 Molecular Biotechnology Division, German Research Centre for Biotechnology, Mascheroder Weg 1, Braunschweig, Germany
232 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...